Fluorescent protein, fusion protein, isolated nucleic acid, vector and application thereof

Abstract

The invention discloses a fluorescent protein, a fusion protein, an isolated nucleic acid, a vector and application thereof, belonging to the technical field of biomedical optics and molecular imaging. The fluorescent protein disclosed in the invention further comprises the amino acid mutation site C158D compared with the conventional fluorescent protein mCrimson. The mutation site enables the fluorescence spectral properties of the fluorescent protein of the invention to be changed, and the fluorescent protein has substantial Stokes shift characteristics, is applicable to single-photon or multi-photon microscopic multicolor imaging, can effectively reduce crosstalk between the fluorescence spectra of different fluorescent proteins, enhances the sensitivity of imaging, ensures simultaneousfluorescent imaging of the fluorescent proteins with different spectra and reduces high requirements on optical devices.

Description

technical field [0001] The invention relates to the technical field of biomedical optics and molecular imaging, in particular to a fluorescent protein, fusion protein, isolated nucleic acid, carrier and application. Background technique [0002] When using fluorescent proteins for in vivo imaging, it is often difficult to achieve multi-channel simultaneous imaging. If this limitation can be broken through, the detection of multiple biological rapid response events can be realized, which will greatly promote the development of neuroscience. [0003] In single-photon imaging, multiple fluorescent proteins can be excited by light with different excitation wavelengths to achieve multi-channel simultaneous imaging. However, in multiphoton imaging, the cost of adding a secondary Ti:sapphire laser or optical parametric oscillator is high, and it is difficult to tune them together, so simultaneous imaging of multiple fluorescent proteins is difficult to achieve. [0004] Current mi...

AI Technical Summary

Problems solved by technology

However, in multiphoton imaging, adding a secondary Ti:sapphire laser or optical parametric oscillator is expensive and difficult to tune together, so simultaneous imaging of multiple fluorescent proteins is difficult to achieve

[0004] Current microscopy methods for simultaneous imaging of multiple biomolecular events mainly rely on dynamic modulation of laser light, requiring multiple sets of repetitive optical devices, which are expensive and difficult to achieve precise synchronization control

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