10 results about "Redox status" patented technology

This method provides in situ quantification of the overall mitochondrial redox state, the redox state of individual cytochromes such as complex III and IV, and methods for treatment should the redox state be identified as hyperoxidized. Mitochondrial rescue from IRI-mediated injury can be achieved using methylene blue, which acts as an alternate electron donor to bypass complex III.

The invention discloses an in-vitro prediction method for cell DNA damage caused by simulated radiation. According to the invention, by introducing the bionic oxidative stress regulation and control module, the simulation precision of the in-vitro model on the in-vivo physiological microenvironment is improved. The dynamic microenvironment intervention system can respond to oxidative stress changes in the radiation process in real time, the redox state of cells is closer to an in-vivo real radiation exposure scene by precisely regulating and controlling the perfusion rate of the active oxygen scavenger, experimental deviation caused by out-of-control oxidative stress level in traditional static culture is avoided, and the experimental accuracy is improved. A more reliable physiological basis is provided for subsequent damage signal detection, a dynamic regulation and control mechanism of the micro-fluidic chip can effectively correct baseline drift caused by oxidative stress fluctuation, and it is ensured that damage site space distribution data captured by the nano biosensor array has a higher signal-to-noise ratio; the influence of external environment interference on experimental data is reduced, and high-quality original data support is provided for follow-up repair trajectory tracking and neural network prediction.

[Problem] To provide a method and a device for obtaining the redox state of an aqueous solution as a hydrogen electrode potential. [Solution] The hydrogen electrode potential of an aqueous solution is defined using the Nernst equation. Comprises: a step for measuring the hydrogen partial pressure and temperature of a gas phase in an equilibrium state with an aqueous solution, and the pH of the aqueous solution; and a step of analyzing the hydrogen electrode potential on the basis of the measured antilogarithm pH 2 of the hydrogen partial pressure and the temperature and pH. For example, at room temperature of 25 DEG C (T = 298.15 K) and pH = 7.0, the hydrogen electrode potential can be determined from [number 32] and an approximation equation. When the pH is constant, the change in the hydrogen electrode potential of the aqueous solution can be determined from [number 33] and an approximation equation.

The invention discloses a reclaimed water total chlorine control system and method. The reclaimed water total chlorine control system comprises: an oxidation-reduction state detection module for detecting whether a first oxidation-reduction potential of to-be-treated reclaimed water is lower than a first preset potential threshold, and sending a chlorine adding request signal to a control module when detecting that the first oxidation-reduction potential is lower than the first preset potential threshold; the storage tank liquid level detection module is used for detecting whether the liquid level of the storage tank filled with the sodium hypochlorite is higher than a preset liquid level threshold value or not, and sending a liquid level qualified signal to the control module when detecting that the liquid level of the storage tank filled with the sodium hypochlorite is higher than the preset liquid level threshold value; the control module is used for controlling the sodium hypochlorite metering pump module to be in a starting state when receiving the liquid level qualification signal and the chlorination request signal, so that sodium hypochlorite is added to the to-be-treated reclaimed water based on the sodium hypochlorite metering pump module, the accuracy and timeliness of sodium hypochlorite feeding are guaranteed, and the total chlorine standard reaching rate of the to-be-treated reclaimed water is increased.

The present invention relates to a bifunctional, reversible, quantitative and Golgi-targeted fluorescent probe for real-time monitoring of the dynamics of intracellular redox homeostasis, belonging to the field of fluorescent probes. Its molecular structure is as follows: The probe molecule exhibits red fluorescence. After reacting with ClO-, selenium (Se) is oxidized to selenium oxide (Se=O) and emits green fluorescence. After reacting with GSH, selenium oxide (Se=O) is reduced to selenium (Se) and the red fluorescence is restored, with Golgi targeting. The probe molecule of the present invention can realize the real-time monitoring of the dynamic changes of the ClO- / GSH-mediated redox state in Golgi oxidative stress, and has important application value in the field of biochemical molecular analysis.

The invention provides a composition for improving inflammatory aging and application thereof. The composition is formed by compounding ergothioneine and verbascoside according to the mass ratio of 1: 10-10: 1, the interaction of a binary mixture is calculated by adopting a joint index (CI) method, and the synergistic anti-inflammatory effect of ergothioneine and verbascoside is determined to relieve inflammatory aging. The composition can improve inflammation by inhibiting the levels of cell inflammatory factors IL-1beta, IL-6 and TNF-alpha; the influence on inflammation is weakened by inhibiting the antioxidant activity (ROS, MDA and GSSG) of cells. Furthermore, the composition can inhibit cell ferroptosis in a targeted manner, reduce the lipid peroxidation level and the cell ferrous iron content, reduce cell aging and maintain the redox state of cells. The composition provided by the invention has a remarkable targeted inhibition effect on cell ferroptosis, and has important significance on inhibition of senescence related to ferroptosis and product development.

The present invention relates to a PTEN oxidative inactivation inducer comprising 1,1-diethoxyethane as an active ingredient and use thereof. More specifically, the present invention relates to: a PTEN oxidative inactivation inducer comprising 1,1-diethoxyethane (1,1-DEE) as an active ingredient; and use thereof, for example, a pharmaceutical composition, a cosmetic composition, a food composition, or a feed composition, as a composition for preventing, treating or ameliorating diseases requiring the oxidative inactivation of PTEN, and a method for detecting PTEN redox state.

The invention discloses a reversible near-infrared small-molecule probe and a preparation method and application thereof in the aspect of monitoring the redox steady state of hemangioma, and the probe is prepared by selecting boron dipyrromethene (BODIPY) of a donor-acceptor (D-A) structure, introducing 3, 4-dihydroxy benzaldehyde to the 3-site of a BODIPY skeleton through a vinyl bridge by virtue of a Knoevenagel condensation reaction, and then introducing 3, 4-dihydroxy benzaldehyde to the 3-site of the BODIPY skeleton by virtue of a vinyl bridge, so as to obtain the reversible near-infrared small-molecule probe. And a small molecule probe with reversible response is designed and synthesized. The probe images the redox state in hemangioma by monitoring the dynamic change of superoxide anion (O2 <.->) and glutathione (GSH). The redox probe provided by the invention has the characteristics of specificity and sensitive response, and provides a potential promising tool for researching the treatment mechanism of anti-cancer drugs and illuminating various redox-related pathological processes.

The present invention relates to methods of sensitizing a subject suffering from cancer or a precancerous stage to a treatment with an immune checkpoint inhibitor, and agents for use in sensitizing a subject to such a treatment.

Disclosed herein are compositions and methods for regulating redox status and / or reducing oxidative stress in a subject, the methods and compositions comprising TLR agonists comprising bacterial lysates and / or lysate fractions. Also disclosed are compositions and methods comprising bacterial lysates and / or lysate fractions formulated or administered in combination with one or more other therapeutic or pharmaceutical agents.