197 results about "Cell aging" patented technology

The process of the invention relates to a kit of two cosmetic, pharmaceutical, veterinary and food compositions, designed for slimming or for preventing and / or repairing inflammatory mechanisms, one of the compositions comprising at least one sirtuin activator and at least one HSP activator, and the other composition comprising at least one sirtuin inhibitor and at least one HSP inhibitor.The said compositions are intended to be delivered in a chronomodulated pattern. They are particularly effective in combination with one another for controlling fat tissues, adipose, fibrous or aqueous cellulite, cell aging and inflammatory processes.

Human pluripotent stem cells which are isolated from cut human umbilical cord or placenta and characteristic of cell surface marker CD151+, OCT4+ and CD184−, can adhere to tissue culture plastic and have the potential to differentiate into three germ layers: endoderm, mesoderm and ectoderm. Methods of isolating, purifying and culturally expanding of a population of human pluripotent stem cells and uses for treating diseases caused by cell damage or cell aging, and as a kind of carrier cells in gene therapy are provided.

The invention discloses a method for carrying out in-vitro efficient amplification on natural killer (NK) cells, and application of the method, aiming at solving the problems that the NK cells amplified by the traditional local method are small in amplification times, have cytokine dependency and cell aging, and the like. The method is characterized by comprising the following steps: 1, separating peripheral blood mononuclear cells (PBMCs) from human peripheral blood by using the traditional Ficoll-Paque density gradient centrifugation method; 2, carrying out 100 Grays irradiation treatment on artificial antigen presenting cells, and then storing by means of liquid nitrogen cryopreservation; 3, carrying out co-culture on the PBMCs and the artificial antigen presenting cells, subjected to the irradiation treatment, in a cell culture flask T75 of an RPMI 1640 medium, wherein the mass ratio of the PBMCs to the artificial antigen presenting cells is equal to 1: 2, and 50IU / mL of interleukin 2 is added into the RPMI 1640 medium; replacing the medium with a fresh medium every 2 to 3 days; 4, calculating the number of total cells in the cell culture flask T75 every 7 days, adding the same number of artificial antigen presenting cells subjected to the irradiation treatment into the cell culture flask T75 for stimulating again, and continuously culturing for 21 days to obtain the amplified NK cells. Therefore, after the method is adopted, a great deal of high-purity and high-quality NK cells can be obtained.

The invention discloses a method for monitoring micro-ecologic maturity of extracted stool sample 16s rRNA in intestinal tract of the infant. The method comprises the processes of sample collecting, 16s-rRNA gene sequencing, sequencing data analyzing, intestinal tract microorganism maturity and intestinal tract cell age computing, and use report pushing; the monitoring index of the intestinal tract microorganism maturity is proposed at the first time, the intestinal tract microorganism maturity is the similarity of the infant intestinal tract microorganism and the mother intestinal tract microorganism on the constitution, and the higher the maturity is, the better the flora development is; and the index can reflect the immunity level of the infant. Through the experimental research and thedata analysis, the infant intestinal tract microorganism evaluation can be realized through a method for detecting the hyper-variable region of the stool sample 16s rRNA V3-V4, the method is convenient for sampling, fast in analysis speed, and low in cost; the evaluation result is consistent with the existing research, and the consumption-level intestinal tract microorganism detection becomes possible.

The invention discloses a sub-totipotent stem cell from cut human umbilical cord or placenta, wherein the cell marker is CD151<+>OCT4<+>CD184<->; the cell grows adhering to the wall in a culture container and can differentiate to the body entoderm, mesoblast and ectoderm tissues. The invention also discloses a preparation method of the sub-totipotent stem cell and an application thereof for preparing drug for treating cell injuries or cell aging diseases, and an application thereof as a carrier cell of a gene treatment drug.

The invention discloses an anti-liver cell aging co-fixation electric biomaterial and an application thereof. The anti-liver cell aging co-fixation electric biomaterial is prepared by the following steps of grafting a charged electric material on a polymer culture plate by a photochemical fixation process to obtain a photo-fixation electric material; co-grafting TNF-a and IGF on the photo-fixation electric material, and performing ultraviolet irradiation to obtain a co-fixation electric biomaterial, wherein the charged electric material refers to polyacrylic acid (PAAc) with negative charges, electroneutral polyethylene glycol (PEG) and polyacrylamide (PAAm) with positive charges. The co-fixation electric biomaterial disclosed by the invention can improve the survival rate of human hepatocytes (HSC) and can effectively suppress the aging of the HSC.

The invention discloses an extraction and culture method of human umbilical cord mesenchymal stem cells. The umbilical cord tissue is cut up and digested by collagenase I and then transferred into a culture bottle containing a culture medium for continuous culture. The culture medium is a low-sugar DMEM (Dulbecco modified eagle medium) and is added with fetal bovine serum, fibroblast growth factor, epithelial cell growth factor, cell transcription factor and cholesterol. The extraction and culture method disclosed by the invention can culture human umbilical cord mesenchymal stem cells for a long time while maintaining the activity of the stem cells. Through the invention, the problems of excessively fast cell aging and differentiation in current culture of human umbilical cord mesenchymal stem cells are solved, and the human umbilical cord mesenchymal stem cells with the characteristics of stem cells can be obtained for a long time.

The invention discloses a composite anti-aging dendrobium officinale preparation capable of promoting skin regeneration. The preparation is prepared from, by weight, 10-15 parts of dendrobium officinale freeze-dried powder, 5-8 parts of an usnea extract, 3-5 parts of truffle micropowder, 15-25 parts of gold foil, 1-3 parts of glycerol glycoside, 1-3 parts of cassava polysaccharides, 10-20 parts of a helix aspersa secreted fluid, 5-8 parts of epidermal growth factors, 1-3 parts of amygdalic acid, 1-3 parts of beta glucan, 3-6 parts of a ginkgo leaf extract, 10-20 parts of levorotatory Vc, 35-45 parts of deionized water, 10-18 parts of hyaluronic acid, 3-5 parts of negative oxygen ions, 5-10 parts of ginseng and 5-10 parts of milkvetch roots. The prepared preparation has the very significant effects of promoting skin regeneration and delaying cell senescence.

The invention provides a skin whitening and freckle removing cosmetic containing a rhodiola rosea extract and an active polypeptide combination. The skin whitening and freckle removing cosmetic comprises the rhodiola rosea extract and the active polypeptide combination as well as a matrix auxiliary material, wherein the active polypeptide combination comprises water, glycerol, 1,2-pentanediol, caprylyl glycol, sodium pyroglutamate, nonapeptide-1 and carnosine. The skin whitening and freckle removing cosmetic provided by the invention has the advantages that the rhodiola rosea extract can delay cell aging and inhibit formation of lipofuscins and active oxygen in cells, nonapeptide-1 can stop tyrosinase from being further activated and no lipofuscin is formed, and carnosine can prevent free radicals produced due to smoking from appearing, so that the cosmetic provided by the invention has the effects of preventing senility of skin and whitening the skin; and active prevention and recuperation of traditional Chinese medicines are combined with passive prevention and treatment of western medicines, so that skin immunity is enhanced while prevention is carried out, irritability is low in a treatment process, and effect is obvious.

The present invention relates to a method for minimizing cell aging of a battery (1) and / or a battery (1) comprising an apparatus for minimizing cell aging of the battery (1). For this the following is performed: detection of battery parameters which describe the present state of the battery (1); checking, on the basis of a preset aging model and the battery parameters, whether the state of the battery (1) is an aging state in which the cell aging of the battery (1) is less pronounced in the case of cyclic charging or discharging than in a quiescent state in which there is no charging or discharging of the battery (1); and cyclic partial discharging and partial charging of the battery (1) if the battery (1) is in such an aging state. The method according to the invention and / or the battery (1) according to the invention are characterized by reduced cell aging. This means that such a battery (1) and / or a battery (1) to which the method is applied has an extended life. This results in particular in cost advantages, ecological advantages and improved user friendliness.

The invention provides a preparation reducing wrinkles. The preparation comprises, by weight, 1.5-2.5 parts of platelet rich plasma, 0.1-1 part of sodium hyaluronate and 2-4 parts of stem cell growth factors. According to the preparation reducing the wrinkles, the stem cell growth factors, the sodium hyaluronate and the platelet rich plasma are combined together, effects of promoting cell metabolism and delaying cell aging of the stem cell growth factors and the mechanism of promoting wound healing of the platelet rich plasma are utilized, by means of the mutual matching effect of the stem cell growth factors, the sodium hyaluronate and the platelet rich plasma, texture, collagen and elastic fibers of skin are comprehensively repaired and recombined, the facial wrinkles fade away and are relieved, and therefore the purposes of delaying skin aging, eliminating the wrinkles and resisting aging are achieved. Accordingly, the preparation reducing the wrinkles is high in wrinkle eliminating efficiency and good in wrinkle eliminating effect. The invention further provides a preparing method for the preparation reducing the wrinkles.

The invention discloses a preparation method and an application of human umbilical cord mesenchymal stem cells. The preparation method includes the steps of: performing asepsis treatment to umbilical cord of a full-term caesarean delivered healthy fetus, mechanically cutting the umbilical cord, digesting the umbilical cord through collagenase and performing separation cultivation. In about the third generation, cell shape becomes flat and broad and the cells grow well. The method can culture the cells till the 15th generation, wherein cell division phase is reduced and cytoplasm is loose, there are vacuoles in the cells, and cell aging and proliferation are significantly slowed, so that the third-generation cells have high activity and can be applied in subsequent researching and treatment. The human umbilical cord mesenchymal stem cells are used in therapy of cerebral injury, and improve function recovery after the cerebral injury.

The invention discloses an estimation method and device of a cell aging states and cell a management system. The method comprises steps of acquiring the current parameter value of a preset cell parameter and acquiring current load voltage of the cell; according to the current parameter value, searching corresponding relation data of experiment load voltage of each aging stage and experiment parameters so as to obtain multiple experiment load voltage; comparing the current load voltage with the experiment load voltage, and determining the aging section where the cell is located currently; carrying out counting processing on the aging section where the cell is located currently so as to obtain counting values and judging whether the sum of the counting values of the aging sections reach a preset threshold; if yes, according to the counting value of each aging section, calculating the aging increasing rate of each aging section; and according to the aging increasing rate of each aging section, estimating the cell aging states. According to the invention, a simple and reliable estimation scheme of the cell aging states based on voltage drop rule statistics of the load voltage of the cell is provided and a problem of complex and unreliable estimation of the cell aging states in the prior art are solved.

An in-vitro skill model and keratinocyte cell long-acting culture solution is composed of a basic culture solution, embryonic stem cell extracts and glycolic acid. The basic culture solution is any of K-SFM or MCDB153 or a mixture of F12 and DMEM with the volume ratio being 1:3, wherein BPE, EGF, insulin and CaCl2 are added. By means of the culture solution, the proliferation and differentiation speed of an in-vitro skill model and keratinocyte cells can be effectively balanced, the retention time of the in-vitro skill model which is successfully established can be effectively prolonged to be 7 days, and the in-vitro skill model is used for culturing the keratinocyte cells to the twelfth generation from the original generation. The time requirement of different detection conditions is greatly met, the application field of the in-vitro skill model is expanded, and meanwhile a basic condition is provided for epidermal cell aging and differentiation and other relevant mechanism research. The operating process is simple and convenient, use is convenient, and cost is low.

The invention provides a total synthesis method for natural products such as rape pollen alkali A and caper alkali B with the activity of delaying cell aging. The method comprises the following steps: taking fructose as an initial raw material, performing tosylate formation, pyrrole substitution, pyrrole bi-hydroxymethylation, oxidation and selective deprotection, reducing and performing acid catalysis on intramolecular ketalation, carrying out a Barton-McCombie deoxidation reaction and a benzyl removal reaction, thereby obtaining the final products such as the rape pollen alkali A and caper alkali B. According to the total synthesis method disclosed by the invention, cheap and readily available raw materials are adopted, the environmental pollution caused by the reaction solvents and reagents is slight, the yield is high, and the synthesized alkaloids and analogues thereof can be used for developing novel anti-inflammatory agents and anti-aging drugs.

The invention discloses a composite western medicine formula for treating gout. The composite western medicine is prepared from, by weight per granule, 0.2-0.5 g of sucralfate, 0.01-0.2 g of diclofenac sodium, 0.01-0.1 g of magnesium oxide, 0.1-0.3 g of benzbromarone and 0.2-1.5 g of chitin. According to the composite western medicine formula for treating the gout, the good gout treatment using effect is achieved; the activity of xanthine oxidase can be inhibited by using the benzbromarone, and therefore the concentration of uric acid in human blood is effectively lowered; by using the sucralfate, a layer of a protective film is formed to cover the ulcer surface, the effect of protecting the gastric mucosa is achieved, the using side effects of medicine on patients suffering from gastric ulcer and the like are effectively reduced, and meanwhile the effects of eliminating inflammation and relieving pain are obvious; by using the chitin, the effects of activating cells in a human body, preventing cell aging, promoting a newly-born cell band, adsorbing harmful heavy metal ions in the body, promoting human body detoxifying and the like can be achieved; gout patients can be effectively treated, and use is very convenient.

The invention relates to the field of cosmetic apparatuses, and provides a micro-current cosmetic instrument and a skin care apparatus. The micro-current cosmetic instrument comprises a switch body, amask mold, wires and a plurality of metal particles, the plurality of metal particles are separately embedded in the mask mold, one ends of the wires are connected with the switch body, and the otherends of the wires are connected with the plurality of metal particles in sequence. According to the micro-current cosmetic instrument and the skin care apparatus, the plurality of metal particles areconnected through the wires, when the metal particles and the user skin are in contact, a channel is formed, micro-current is further formed, the micro-current can promote the absorption capability of the skin, the absorption of various skin care products for the skin is improved, the functions of the skin care products can reach the optimal, the metabolism of cells can be promoted, the blood circulation is improved, and the cell aging is delayed; a certain temperature is generated by electrification of the metal particles so that the user skin is massaged, and the absorption capability of the skin is further improved; besides, the skin care apparatus can be used with the cooperation of other devices or other skin care products so that the skin care effect is better.

The invention discloses probiotic fermented germinated acid soybean biscuits and a production process thereof. The probiotic fermented germinated acid soybean biscuits consist of the following components in part by weight: 1.5 to 3 parts of germinated acid soybean dregs, 2.5 to 5 parts of flour, 1 to 1.5 parts of sugar, 1.5 to 3 parts of fermentation liquor, 1 to 1.5 parts of water, 0.5 to 1 partof edible oil, 0.25 to 0.5 part of egg liquid, 0.2 to 0.5 part of milk powder, and 0.05 to 0.1 part of baking soda. The production process comprises the following steps of: 1, soaking for germination; 2, fermenting germinated soybean; 3, obtaining germinated acid soybean dregs and fermentation liquor; 4, blending for the germinated acid soybean biscuits; and 5, molding and baking. By the invention, the content of toxic, harmful or anti-nutrient substances in the soybean can be reduced or removed, the protein digestibility is improved, the content of nutrients such as limiting amino acid and vitamins in the soybean is improved, various amino acids are generated through probiotic fermentation, the digestibility, absorption and nutritional value of the probiotic fermented germinated acid soybean biscuits can be improved, and the probiotic fermented germinated acid soybean biscuits have the effects of regulating the intestines, resisting bacteria, preventing diseases, improving immunity and cancer resistance, and slowing cell aging to prolong life, and have moderate sour and sweet taste, rich bean flavor and mellow fermentation aroma.

The invention discloses a zero-calorie health care type siraitia grosvenorii candy and a preparation method thereof. The preparation method comprises the following steps: according to the weight parts of used materials, weighing 5 parts of fresh siraitia grosvenorii aspartame and 95 parts of erythritol particles at first, and then uniformly mixing the fresh siraitia grosvenorii aspartame and the erythritol particles; spreading the mixture into a 3cm thin layer, and putting the thin layer into a condition with the relative humidity of 70-80% to ensure that the fresh siraitia grosvenorii aspartame absorbs moisture and is adhered to the erythritol particles; then putting the mixture into an environment with the relative humidity of 50%, and mixing again according to a ratio of the fresh siraitia grosvenorii aspartame to the erythritol particles adhered with the fresh siraitia grosvenorii aspartame of 5:100; screening by using a sieve of 100 meshes to remove free fresh siraitia grosvenorii aspartame; then dehumidifying by using an electrostatic dehydration device, and drying under a condition of 45-55 DEG C for 5-8 minutes; and packaging in an environment with the relative humidity of within 50%. According to the preparation method disclosed by the invention, the fresh siraitia grosvenorii aspartame is wrapped on the erythritol particles, and the prepared product is zero-calorie, good in color, luster and mouthfeel and strong in health care effect, and has the effects of improving human immunity, protecting the liver, reducing aminotransferase, delaying cell aging and the like.

The invention relates to cat foods, in particular to a cat food with the skin and hair growth promoting function. The cat food is prepared from, by mass, 2%-4% of Kang factor peptide, 2%-5% of avocado extract, 1%-6% of black bean powder, 6%-10% of egg white powder, 1%-3% of bovine-derived power, 1%-3% of dried small shrimps, 1%-6% of mouse internal organs, 0.5%-1.5% of lysine, 10%-50% of chicken, 1%-3% of deep sea fish oil, 0.5%-1.5% of animal oil, 0.5%-1.5% of folic acid, 1%-2% of fish flesh, 1%-5% of vitamin C, 1%-5% of food additive and the balance wheat meal. By means of the cat food, metabolism of skin cells can be promoted, cell aging can be delayed, skin elasticity can be enhanced, hair drying-off can be avoided, growth of new hair can be promoted, the morbidity of skin diseases can be reduced, and skin allergy and pruritus can be relieved.

Disclosed is a cell aging method. The cell aging method comprises the following steps of taking an electrolyte-injected cell and horizontally putting the cell on a rotatable bearing platform, and enabling an airbag of the cell to face to the center of the bearing platform in a radial direction; enabling the bearing platform to continuously rotate at a first rotary speed v1; enabling the bearing platform to continuously rotate at a second rotary speed v2, wherein v2 is greater than v1; enabling the bearing platform to continuously rotate at the first rotary speed v1; and gradually decreasing the rotary speed of the bearing platform until the bearing platform stops rotating, performing standing on the cell, and taking off the cell after aging is finished. According to the cell aging method, the cell is rotated at a low speed and then at a high speed in the aging process, so that an electrode material can be fully wetted by an electrolyte, a sufficient reaction occasion for the electrochemical reaction of the cell is provided, and the electrolyte retaining amount of the cell is improved.

The invention discloses an eye patch composition, which is prepared from the following components by weight: 40-110 parts of baikal skullcap roots, 40-110 parts of herb of fineleaf schizonepeta, 40-110 parts of Chinese angelica roots, 50-150 parts of phellodendron bark, 50-150 parts of chuanxiong rhizomes, 10-30 parts of wild chrysanthemums, 10-30 parts of pipewort, 50-150 parts of ptunella spike,10-30 parts of dendrobium stems, 40-110 parts of seeds, 10-30 parts of wolferry fruits, 10-30 parts of blueberry extract, 50-150 parts of red sange roots, 5-8 parts of borneol, 1-4 parts of hyaluronic acid, 50-150 parts of peony bark, 40-110 parts of menthol, 10-30 parts of hydrolyzed pearl liquid, 5-10 parts of vitamin B12, 2-6 parts of methylparaben and 10-30 parts of snail secretion filtrate.The invention provides the eye patch composition and a preparation method thereof. The composition adopts components with strong solubility, and the components can activate human cells after absorption, and solve the problem of cell aging and cell viability caused by long-term use of the eyes, improve the vitality of the human eye cells and reduce the fatigue of the human eyes.

The invention discloses a composition containing stem cell components and application thereof. Based a total mass fraction of 100%, the composition comprises 20-100% of a stem cell concentrated solution and 0-80% of a complete medium, and vitamin E and ginseng extract are added into the composition. Oxidation resistance of the product is determined by an anti-oxidation experiment, an oxidative stress cell aging model is established, and the stem cell composition is subjected to anti-aging function study, so that the stem cell composition with multiple effects, small toxic and side effects andan anti-aging effect is found.

The invention relates to a manufacture and a detection method of lucid ganoderma liquid strains. The method comprises the following steps: firstly, purifying a lucid ganoderma mother strain in a mother strain purification culture medium, inoculating the purified mother strain into a shake flask to be cultured in a liquid culture medium, taking out the cultured liquid culture medium, and inoculating the cultured liquid culture medium into a fermentation tank for continuous culture, and using the obtained liquid strain after the obtained liquid strain is qualified through detection. The mother strain purification culture medium, the liquid culture medium and a fermentation tank culture medium all have various formulas and can be flexibly selected according to situations. The liquid strain prepared from the method has the characteristics of consistent cell age, high permeability, short production period, early sprouting and high material absorbing speed.

The invention relates to a daily cleaning product, and particularly relates to a handmade soap. The handmade soap comprises the following raw material components: olive oil, sunflower seed oil, coconut oil, palm oil, sodium hydroxide, water and lavender essential oil, wherein the olive oil contains a large amount of oleic acid and polyphenol; the polyphenol is an antioxidant substance and is capable of eliminating facial wrinkles, inhibiting cell aging, preventing skin aging, resisting carcinogenic substances and protecting skin; and the lavender essential oil is capable of equilibrating grease secretion and conditioning the skin and has the effects of protecting the skin, relieving nervous tension, decompressing and calming. Thus, the handmade soap disclosed by the invention not only enables the skin to be moisturized, glossy and full of elasticity, but also has the effects of eliminating wrinkles, whitening, moisturizing and preserving moisture, is low in cost, does not contain chemical components, namely a preservative agent and a surface active agent, is a very safe and effective skin cleaning and beautifying product and is very suitable for children and pregnant women who have poor immunity.

The invention discloses an anti-light aging preparation for the skin, wherein the preparation comprises royal jelly acid (10-HAD) and AA-2G. Meanwhile, the invention further discloses application of royal jelly acid and AA-2G in preparation of skin care products, cosmetics and cosmeceuticals. The anti-light aging preparation can substantially improve activity of cells irradiated by UVA, inhibits UVA-induced ROS generation and UVA-induced cell aging and has a substantial anti-light aging effect. According to research on action mechanism of the anti-light aging preparation, 10-HDA included in the anti-light aging preparation can inhibit activation of p-JNK and p38 MAPK signaling pathways and reduce expression of UVA-induced MMP-1 and MMP-3, so the content of collagen in cells can be increased; thus, the preparation is an effective anti-light aging preparation for the skin.