79 results about "Cell aging" patented technology

The invention relates to the cross technical field of biological medicine and image processing, and discloses a mesenchymal stem cell aging detection method based on image processing. The method comprises the following steps: automatically collecting bright field and multi-channel fluorescence images in an integrated cell culture monitoring device; after background correction and illumination homogenization, segmenting the cells by using a U-Net network and extracting single cell contours; calculating characteristics such as cell area, roundness, cytoplasmic ratio, nuclear form irregularity index, lysosome fluorescence intensity mean value and distribution entropy; and inputting a gradient boosting decision tree model to judge the unicellular aging state, and evaluating population aging with a 20% positive rate threshold. According to the invention, label-free, non-invasive and high-flux accurate detection is realized, and the method is superior to manual interpretation.

The invention relates to the technical field of battery cell capacity fault prediction, and particularly discloses a battery fault prediction method and system based on a hierarchical health assessment model, and the method comprises the steps: obtaining an operation historical data set and an initial capacity; performing aging stage division on the operation historical data; constructing a three-level prediction model; outputting a capacity prediction result set; constructing a dynamic residual sequence in combination with the stage sensing information and the long-short-term memory network; and outputting an alarm result. Compared with the prior art that the difference of the aging stages of the battery cell is ignored, and particularly, the technical problems that the model prediction error is large and early warning is not timely under the condition that the battery cell enters the later stage of nonlinear capacity degradation are solved. The prediction adaptability and sensitivity in each stage are effectively improved, and the accuracy and timeliness of battery cell fault early warning are enhanced.

The invention provides a new energy automobile battery rapid detection method and device, and the method comprises the steps: extracting the discrete characteristics of the battery capacity in a to-be-detected battery pack from electrical parameter data, determining the charge state difference of each single battery in a charging process, and extracting the temperature rise characteristics of each single battery from temperature data; determining a monomer aging index of the corresponding battery monomer according to the charge state difference and the temperature rise characteristic of each battery monomer, and further determining the overall aging characteristic of the battery pack to be tested; determining the relative aging gradient of each battery monomer based on the charging and discharging duration of the battery pack to be detected and all monomer aging indexes; and determining a health state detection value of the to-be-detected battery pack through all the relative aging gradients and the overall aging characteristics, and calibrating the health state of the to-be-detected battery pack based on the health state detection value. By adopting the scheme of the invention, the battery pack can be rapidly detected based on the aging of the monomers in the battery pack and the consistency in the battery pack.

The invention discloses an oral hard capsule health-care product with an anti-aging function as well as a preparation method and application of the oral hard capsule health-care product. The health-care product is mainly prepared from eight active ingredients including EGT (Ergothioneine), Ca-AKG (alpha-ketoglutarate), PQQ (pyrroloquinoline quinone), fisetin, salidroside, urolithin A, spermidine and an apple stem cell extract. Through scientific compatibility of the various components, the composition disclosed by the invention can comprehensively intervene in a cell aging process through a synergistic effect of a plurality of pathways of enhancing the cell antioxidant capacity, improving the mitochondrial function, activating cell autophagy, promoting cell regeneration and repair and the like. The invention also provides a preferred content range of the health-care product, a preparation method of the health-care product and application of the health-care product in preparation of health-care foods for delaying senescence, improving cell viability or improving mitochondrial functions. The product provided by the invention has the characteristics of multiple targets and synergistic interaction, and provides a new effective scheme for anti-aging intervention.

The invention discloses a method for inducing fibroblasts to be reprogrammed into mesenchymal matrix-like cells (MLCs) by using small chemical molecules and application of the mesenchymal matrix-like cells (MLCs) in the anti-aging field. The invention discloses a set of specific chemical small molecule composition and an induction scheme thereof, which can be used for efficiently reprogramming fibroblasts from somatic cells into MLCs. Furthermore, the huge potential of the MLCs obtained by the method in anti-aging research is deeply explored, and the effect of the MLCs is proved to be obviously superior to that of umbilical cord mesenchymal stem cells in the aspects of reversing cell aging phenotypes and recovering tissue functions. The invention not only provides a new stem cell source strategy for avoiding the ethical and safety problems of the traditional reprogramming technology, but also lays a solid cell foundation for developing a novel, safe and effective anti-aging therapy based on cell transplantation or secreted factors.

The application discloses a lithium battery protection plate cell voltage equalization and protection threshold adjustment method, and relates to the technical field of lithium battery protection plates.The application synchronously completes online estimation of a state of charge SOC and a state of health SOH of a cell and analysis of cell dispersion by collecting electrical parameters and working condition data of each single cell of a battery pack in real time;based on charging, discharging, and standing full working condition division, differential dynamic equalization control is executed, and cell equalization state, SOH dispersion, real-time working condition, and temperature data are linked to adaptively and dynamically adjust overcharge, overdischarge, overcurrent, and other protection thresholds;finally, through a whole-process closed-loop control of collection-estimation-equalization-threshold adjustment-closed-loop verification, the problems that cell equalization and protection threshold control are independent of each other and cannot adapt to cell aging and consistency differences in the prior art are avoided, protection mis-triggering and cell over-stress damage are avoided, and the cycle service life and operation safety of a lithium battery pack are significantly prolonged.

The invention belongs to the field of food science and engineering, and discloses a preparation method of an active medicinal and edible composite nutrient AMCN with an anti-aging effect, and the preparation method comprises the following steps: putting a ganoderma lucidum monoclonal strain into a liquid culture medium for controllable culture to obtain supernate with beta-glucosidase activity of over 100mU / mL; and adding rhizoma polygonati powder, lucid ganoderma powder and mulberry leaf powder, carrying out enzymatic biotransformation, carrying out lactobacillus plantarum fermentation, adding a freeze-drying protective additive, carrying out freeze-drying, grinding to obtain the active medicinal and edible composite nutrient AMCN, and storing the finished product in a dry and dark place. The active medicinal and edible composite nutrient can be used for preparing anti-aging functional foods or nutritional health-care products. A D-galactose induced zebrafish aging model verifies that the active medicinal and edible composite nutrient prepared by the application plays an anti-aging role by relieving oxidative stress, inhibiting cell aging and maintaining telomere stability, can reduce beta-galactosidase activity and intracellular ROS (reactive oxygen species) level, improves telomerase protein concentration, and has a good anti-aging effect. And the telomere length can be effectively recovered by medium and high concentrations.

The curcumin-coupled copper-gold bimetallic nanoszyme AuCu-Cur prepared by the application can remove ROS and relieve oxidative stress, prevent further damage to DNA, and introduce human umbilical cord mesenchymal stem cell-derived extracellular vesicles (hUCMSC-EVs) to improve the bioavailability. In the system, curcumin can repair damaged DNA by promoting the expression of NEIL3. In addition, the vesicles themselves are rich in various tissue repair-related proteins and RNAs, which can promote DNA replication of notochordal cells (NPCs), reverse the aging state and stimulate extracellular matrix synthesis. In the IVDD model induced by rat tail vertebra puncture, we confirmed that the material has strong antioxidant and DNA repair capacity and can effectively delay cell aging, thereby relieving the progression of IVDD. Compared with existing intervertebral disc biomaterial treatments, the present scheme can realize continuous DNA repair, which is an active, multi-target repair program that not only interrupts the vicious cycle of intervertebral disc degeneration but also promotes tissue transformation to regeneration.

The invention provides an SOC-OCV model construction and test system and method under multi-temperature static charging and discharging, and relates to the technical field of battery state-of-charge estimation, and the method comprises the steps: obtaining the operation data of a single cell in the static charging and discharging process at different temperatures, and carrying out the preprocessing of the operation data; calculating a dynamic SOC based on an ampere-hour integral method by using the preprocessed operation data, eliminating an integral error through standing OCV calibration, fitting SOC-OCV data at each temperature by using a fourth-order polynomial based on a standing SOC-OCV data pair, and constructing a multi-temperature OCV model; setting a target temperature, calculating an OCV difference value between the target temperature and a reference temperature, realizing SOC rapid correction based on the reference temperature, and updating OCV model parameters according to the cell aging data; and testing the multi-temperature OCV model at each temperature, calculating fitting OCV of SOC points, outputting SOC and OCV predicted values, and storing test data. According to the invention, accurate estimation of the state of charge (SOC) of the battery in different temperature environments is realized.

The invention discloses an application of an engineered mRNA (messenger Ribonucleic Acid) medicine for coding COL3A1 protein in resisting skin photoaging. According to the method, accurate rational design and optimization are carried out on the 5'untranslated region (UTR) of the hCOL3A1 mRNA, so that the translation efficiency and the protein yield of the hCOL3A1 mRNA are greatly improved, the expression level of the hCOL3A1 target protein far beyond the conventional sequence in vivo and in vitro is realized, and the type III collagen is efficiently and endogenously synthesized in skin cells. Experimental results show that the engineered mRNA can effectively reduce the skin oxidative stress level, inhibit cell aging and promote endogenous synthesis and deposition of corium layer collagen, so that the barrier function of photoaged skin is remarkably improved, the dermis thickness is increased, the tissue structure is repaired, and systemic toxicity or immune side effects are not observed. The invention provides a novel strategy with high efficiency and safety for resisting skin photoaging.

This invention provides a method for constructing a human endometrial organoid aging model, belonging to the field of biological model technology. Based on human endometrial organoids, this invention utilizes hydrogen peroxide (H2O2) stimulation to induce a human endometrial organoid aging model. This human endometrial organoid aging model clearly exhibits multiple cellular aging phenotypes, including slowed organoid growth rate, decreased cell proliferation index (Ki67), enhanced SA-β-gal activity, upregulated expression of cell cycle inhibitors P16 and P21, and significantly upregulated gene expression levels of aging-associated secretory phenotype (SASP) components. It can provide an effective tool for screening anti-aging drugs for women, high-throughput and high-content screening, personalized treatment strategy development, and mechanistic research.

The invention relates to plant source dry-state mitochondria for cell repair and aging resistance as well as a preparation method and application of the plant source dry-state mitochondria. The plant source dry-state mitochondria can effectively improve functions of mitochondria, reduce the damage degree of cells, inhibit cell aging and effectively repair skin wounds. According to the method, the mitochondria is extracted from healthy plant tissues, so that the production cost is reduced, and a harsh quarantine step is not needed. According to the present invention, the dry-state processing enables the mitochondria storage condition to be loose, the transportation and storage difficulty and the transportation and storage cost can be further reduced, the plant source mitochondria subjected to the dry-state processing has characteristics of flexible dosage form, wide auxiliary material application range, and meeting of the design requirements of various products, and the dry-state processing can further remove the nucleic acid in the extract and eliminate the inflammation of the product; in the research process, the inventor also finds that after the plant source mitochondria is subjected to dry-state processing, the mitochondria function can be more effectively improved, the cell damage degree is reduced, cell aging is inhibited, and the skin wound surface is effectively repaired.

The invention belongs to the technical field of crystal engineering, and discloses a lutein-glutamic acid eutectic crystal and a preparation method and application thereof, Cu-k alpha radiation is used, and the lutein-glutamic acid eutectic crystal has characteristic peaks at 21.75 + / -0.2 degrees, 25.95 + / -0.2 degrees, 31.34 + / -0.2 degrees and 35.96 + / -0.2 degrees in an X-ray powder diffraction pattern represented by a diffraction angle 2 theta. According to the lutein-glutamic acid eutectic crystal provided by the invention, on the premise of not changing the pharmacological activity of the lutein, the solubility and the dissolution rate of the lutein are remarkably improved, and the stability of the lutein-glutamic acid eutectic crystal under the conditions of high temperature, high humidity and strong light is greatly enhanced; in-vitro cell experiments further prove that compared with pure xanthophyll, the xanthophyll-glutamic acid eutectic has more excellent cell aging resistance, oxidation resistance and vasodilation promoting activity, and an ideal dosage form is provided for efficient application of xanthophyll in medicines and functional foods.

The invention provides an application of a component A as or used for preparing an SIRT5 inducer, the component A at least comprises one or more than two of ergothioneine, ectoine and hyaluronic acid or salt thereof, and the component A can be used for the SIRT5 inducer or used for preparing an SIRT5 expression inducer, so that cell aging can be inhibited, and the SIRT5 inducer can be used for preparing an SIRT5 expression inducer. The traditional Chinese medicine composition is used for improving natural skin aging, cardiac hypertrophy and / or hyperammonemia.

The invention discloses an automatic internal resistance and voltage detection device and method for lithium ion cell aging treatment, and belongs to the technical field of lithium battery production. The turntable is horizontally arranged and is driven by an intermittent rotating mechanism to rotate, a plurality of battery cell fixing grooves are fixed on the upper surface of the turntable, a vertically arranged probe bracket is arranged on the outer side of the turntable, the lower end of the probe bracket is fixedly connected with the rack, and the upper end of the probe bracket is fixedly connected with a cylinder body of a vertically arranged air cylinder; a piston rod of the air cylinder is fixedly connected with a test probe, and the test probe is connected with a corresponding acquisition sensing line and is correspondingly matched with the battery cell fixing groove. According to the invention, manual operation is replaced, human resource configuration is optimized, accuracy and repeatability of detection results are improved, programmed execution of detection standards is realized, the problem of inconsistent standard execution caused by human factors is avoided, cell grouping and pairing precision is improved, and cell performance consistency control is guaranteed.

The application discloses an ALKBH5-promoted cell aging mechanism and an RNA epigenetic regulation cell aging preparation, relates to the technical field of biological medicine, and aims to solve the technical problem of lack of ALKBH5-regulated cell aging mechanism in the prior art. The mechanism is that retention of ALKBH5 in the cytoplasm promotes cell aging; retention of ALKBH5 in the cytoplasm leads to m6A disorder, and further causes cell aging; aggregation of ALKBH5 in the cytoplasm causes cytoplasm retention, leads to m6A disorder and m6A hypermethylation of Cdk2, and further causes Cdk2 RNA instability, and further causes cell aging; a key mediating factor for ALKBH5 entering the nucleus is Nup62; by transfecting an RNA probe containing m6A modification into cells, it is found that m6A-labeled RNA can effectively reverse cytoplasmic aggregation of ALKBH5; and an adeno-associated virus of NLS-labeled ALKBH5 is used for supplementing ALKBH5 to the nucleus in vivo, so as to inhibit cell aging.

The application provides a composition and application thereof in preparation of a mitochondrial function regulator, and relates to the technical field of hyaluronic acid grafts. It is found for the first time in the application that N-sodium hyaluronoyl glutamate can effectively improve or restore mitochondrial function, improve cell energy metabolism, and achieve the effects of improving cell aging and / or improving cell activity. A new efficacy component is provided for developing a mitochondrial function regulator, a product for preventing and / or improving aging; further, N-sodium hyaluronoyl glutamate can break the plateau period of the action of ergothioneine, and provides a more effective scheme for further reversing mitochondrial function.

The application discloses an engineered exosome targeting human urokinase-type plasminogen activator receptor uPAR and a preparation method and application thereof. Firstly, a high-affinity anti-human uPAR single-domain antibody VHH is prepared; then, a human umbilical cord mesenchymal stem cell engineering cell line expressing a VHH-PTGFRN fusion protein is constructed, and the engineered exosome is prepared and purified by combining three-dimensional hypoxic culture, chemical induction and size-exclusion chromatography technology. The engineered exosome comprises four comparative samples, wherein the core sample Exo^(VHH / miR) can simultaneously display the anti-uPAR VHH on the membrane surface to realize targeted delivery and enrich miR-193b-3p in the chamber to realize gene silencing, and the synergistic effect of the two can efficiently remove the uPAR high-expression senescent cells and inhibit the senescence-associated secretory phenotype SASP. The engineered exosome has the advantages of standardized preparation process, high yield and high purity, strong targeting, excellent curative effect and good safety, and can be used for preparing a treatment preparation for anti-aging and aging-related diseases, and has important clinical application value.

The invention belongs to the technical field of cell culture, and particularly relates to a bone marrow mesenchymal stem cell culture medium and a preparation method and application thereof. The mesenchymal stem cell culture medium comprises a basic culture medium, and betaine, glycyrrhizin, insulin, EGF (Epidermal Growth Factor), L-propyl dipeptide and naloxicol which are added into the basic culture medium. The bone marrow mesenchymal stem cell culture medium can effectively improve the multiplication capacity of bone marrow mesenchymal stem cells, delay cell senescence and improve the osteogenic differentiation capacity of the bone marrow mesenchymal stem cells, fetal calf serum is not added, the risk of introducing heterologous serum to carry viruses is avoided, and the safety of clinical application is improved.

The application is based on the pathology of vascular injury, and uses cryptochlorogenic acid as a raw material in vascular endothelial cell aging, migration and tube formation for the treatment of Alzheimer's disease; the application of cryptochlorogenic acid in the preparation of a drug for treating or preventing vascular endothelial cell barrier dysfunction caused by Alzheimer's disease, wherein the only active ingredient in the drug is cryptochlorogenic acid, and the drug is used for: (i) inhibiting vascular endothelial cell aging, restoring the migration ability of vascular endothelial cells, and restoring the tube formation ability of vascular endothelial cells; (ii) inhibiting the damage of Alzheimer's disease serum to the vascular endothelial cell barrier.

The application discloses application of serine or a pharmaceutically acceptable salt thereof in preparation of a medicine for preventing or treating intervertebral disc degeneration, and a medicine composition containing the active ingredient, and belongs to the technical field of biological medicine. The application takes serine as the active ingredient, provides application of the serine in preparation of the medicine for treating intervertebral disc degeneration, the medicine can supplement serine exogenously, corrects redox imbalance of nucleus pulposus cells, reverses cell aging, and thus can improve the intervertebral disc degeneration process. The application further provides a corresponding medicine composition, which can be prepared into a local injection preparation or an oral preparation, wherein the local injection preparation can be combined with a biomaterial to construct a slow-release delivery system, so that long-acting targeted drug delivery is achieved.

The invention relates to the field of biological detection, in particular to a multi-omics combined screening cell senescence marker detection method and application thereof. According to the method, data of genomics, transcriptomics, proteomics and metabonomics are combined, and multi-dimensional data in the cell senescence process are subjected to high-throughput screening and integration by means of whole genome sequencing, RNA-Seq, a liquid chromatography-mass spectrometry technology and a gas chromatography-mass spectrometry technology. And adopting machine learning algorithms such as random forest, a support vector machine and deep learning to screen out key markers related to aging. The markers comprise genes, proteins and metabolites, and have relatively high sensitivity and specificity. Verification of different aging models proves that the screened markers change significantly in the aging process and can effectively reflect the biological characteristics of cell aging. The method provides new technical support and theoretical basis for research of an aging mechanism, early diagnosis of aging-related diseases and anti-aging treatment.

The invention relates to the technical field of biological medicines, and particularly discloses application of formononin in preparation of a medicine for regulating and controlling BNIP3-mediated excessive mitochondrial autophagy. The invention discloses that ononin can be used as a direct small molecule inhibitor of BNIP3 protein, specific binding of ononin with BNIP3 is verified through molecular docking, surface plasmon resonance and molecular dynamics simulation in multiple dimensions, and in an iron overload induced ovarian granulosa cell injury model, the ononin can be used as an iron overload induced ovarian granulosa cell injury model. The formononin effectively inhibits mitochondrial excessive autophagy mediated by a BNIP3-PINK1 / Parkin axis through targeting BNIP3, remarkably recovers mitochondrial membrane potential, reduces mitochondrial active oxygen level and improves cell oxygen consumption rate (OCR), so that stressful high-flux and low-efficiency breathing states are corrected, cell senescence phenotypes are delayed, and cell apoptosis is inhibited. And a new small molecule drug strategy with a clear structure and a clear action mechanism is provided for iron overload related ovarian function impairment.

A prophylactic or ameliorating agent for cell aging, comprising 2'-fucosyllactose.

The application belongs to the technical field of genetic engineering and cell modification, and discloses a genetic engineering construction method and application of an anti-aging human mesenchymal stem cell. Based on eliminating R-loop formed in the process of cell aging from the source, relieving the adverse effects of genome instability caused by R-loop, the application develops RNH1 overexpression virus transfection MSC aiming at eliminating cell R-loop. The RNH1 overexpression MSC provided by the application exhibits significant anti-aging activity, has strong environmental adaptability and excellent safety characteristics, can effectively resist harsh in-vivo inflammation and aging microenvironment, and avoid the tumorigenic risk after cell transplantation, and the MSCs with stable anti-aging ability can be applied to significantly improve the curative effect of MSCs infusion in-vivo disease treatment.

The invention relates to the technical field of cosmetics, in particular to a skin care composition with a skin aging improving function, which comprises mogroside V and a golden camellia extract. The composition can significantly reduce the expression level of aging-related SA-beta-galactosidase in human skin fibroblasts, thereby effectively inhibiting skin cell aging. Mogroside V and the camellia nitidissima extract are compounded to achieve a synergistic effect, and the anti-aging effect of the composition is remarkably superior to that of a single component. The composition is safe and non-irritant, the raw materials are natural, and the composition is suitable for various cosmetic dosage forms such as essence, face cream and facial masks.

The application discloses a NAND flash memory cell aging state evolution prediction method and system based on a graph neural network, comprising the following steps: first, obtaining the state attributes and physical adjacency relationship of each memory cell in a NAND flash memory array, and mapping the flash memory array into a graph data structure, wherein the memory cell is a node, the physical adjacency relationship is an edge, and the state attribute is a node feature; then, inputting the graph data structure into a pre-trained space-time evolution graph neural network model, the model being internally provided with a space information aggregation module and a time state updating module, so as to learn and infer the aging state prediction result of each memory cell at a future time point; finally, a storage controller executes a forward-looking reliability management operation according to the prediction result. The application overcomes the defect that the traditional method cannot accurately model the space-time evolution rule, and can realize cell-level and high-precision aging state prediction, thereby effectively prolonging the service life of the flash memory.

The invention provides a whole-plant purple corn anthocyanin substitutional tea formula and a preparation process thereof, and relates to the technical field of substitutional tea processing, and the whole-plant purple corn anthocyanin substitutional tea formula is prepared from the following raw materials: a whole-plant purple corn raw material, purple corn stigma, medicinal and edible auxiliary materials, a natural sweetening agent, an anthocyanin protective agent, citric acid and beta-cyclodextrin. The beverage has strong antioxidant ability, can effectively remove free radicals in the body, slow down the cell aging process and prevent various chronic diseases, and meanwhile, anthocyanin also has the effects of resisting inflammation and protecting eyesight, can relieve eye fatigue and prevent eye diseases, and meets the requirements of consumers on health and health preservation; the anthocyanin protective agent and the beta-cyclodextrin are added, so that the substitute tea can still keep higher anthocyanin content after being stored for a long time, and the nutritional value and the efficacy of the product are maintained.

The technical field of the application is a device for medical, oral or dental hygiene. The application discloses a hydrogel material and a preparation method and application thereof. The preparation method of the hydrogel material comprises: preparing a ZnSe / rGO heterojunction by a hydrothermal method through ZnSe and rGO, wherein the ZnSe is a semiconductor material zinc selenide, and the rGO is a conductor material reduced graphene oxide; and uniformly dispersing the ZnSe / rGO heterojunction in a GelMA matrix, and loading and fixing the ZnSe / rGO heterojunction on the GelMA to obtain a hydrogel material Z / r@G, wherein Z refers to ZnSe, r refers to rGO, and G refers to GelMA, and the GelMA is methacrylated gelatin. The application develops a preventive strategy capable of delaying or reducing cell aging, which can not only avoid the influence of excessive removal of aged cells on later bone repair, but also widely exert an anti-aging effect.

The invention relates to a method and a system for designing a battery module. The method for designing the optimal battery module comprises the following steps: receiving target design information about a target battery module, wherein the target battery module comprises a target battery monomer; predicting an aging of the target battery cell based on the target design information by using a cell aging prediction model associating a design of a battery module including the battery cell with the aging of the battery cell; and determining whether the target design information is feasible based on the target design information and the predicted aging of the target battery cell.