198 results about "Cell aging" patented technology

The invention discloses a high-sensitivity detection method for the safety of a battery cell, and the method comprises the following steps: S1, collecting the data of the battery cell in real time through a tunnel magnetoresistive sensor, and outputting a preliminary detection data set; s2, generating a micro-short circuit risk identification signal based on the preliminary detection data set; s3, generating a temperature anomaly distribution map by using the preliminary detection data set; s4, generating a thermal runaway early warning signal based on the temperature anomaly distribution map; s5, performing magnetic field scanning on the pole piece to generate a magnetic distortion data atlas; s6, constructing battery cell multi-source detection data, inputting the data into the physical information neural network, and training to obtain a final battery cell aging evolution model; s7, outputting a charging and discharging strategy optimization instruction based on the final cell aging evolution model; and S8, uniformly fusing the micro short circuit risk identification signal, the thermal runaway early warning signal and the charging and discharging strategy optimization instruction to generate a comprehensive response control instruction. According to the method, magnetic resistance sensing and physical modeling are fused, and intelligent prediction and response control optimization of the risk of the battery cell are realized.

The invention provides a strain of lactobacillus plantarum YS-AG23 with high antioxidant activity, and a rhizoma polygonati and radix angelicae sinensis fermented product obtained by fermenting rhizoma polygonati and radix angelicae sinensis with the strain of lactobacillus plantarum YS-AG23. In a D-galactose induced premature ovarian failure mouse model, the fermentation product can regulate up the levels of antioxidant and stress protection genes SOD2 and HO-1 and an anti-aging gene SIRT1 by regulating down the expression of a key gene AGER of an inflammation and oxidative stress pathway, so that the oxidative stress and inflammatory response of model mouse ovarian tissues are remarkably reduced; meanwhile, the serum hormone level can be specifically adjusted, the serum estradiol (E2) level can be increased, the follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels can be reduced to be close to the normal range, the expression of anti-mullerian hormone (AMH) and hormone regulatory gene LHR of ovarian reserve markers can be up-regulated, the proliferative activity and estrogen secretion function of follicular granulosa cells can be improved, and the effect of preventing ovarian reserve is achieved. The wet weight of atrophic uterus and ovary of the model mouse is remarkably increased, uterine and ovary atrophy is effectively reversed, and the ovarian reserve function of the D-galactose induced premature ovarian failure model mouse is recovered. Besides, in an H2O2-induced human dermal fibroblast aging model, the yeast can improve the activity of aging cells in a concentration-dependent manner and reduce the level of reactive oxygen species (ROS) in the cells; in addition, by up-regulating the expression of a collagen synthesis key gene COL1A1 and down-regulating the expression of a cell senescence marker gene p16, skin cell senescence caused by oxidative stress can be effectively improved. In conclusion, the polygonatum sibiricum and angelica sinensis leavening can synchronously improve premature ovarian failure and skin cell senescence caused by oxidative stress by accurately regulating and controlling oxidative stress, inflammatory response and tissue function related genes, and has wide application potential.

The invention belongs to the technical field of cell biology, and particularly relates to a method for detecting cell stability, a cell sample is detected in three aspects of cell performance, cell mutation and cell stability, and when the cell proliferation effect is good, the cell does not migrate, and the cell activity is gt; the cell of which the cell concentration is 80%, the cell early-stage and late-stage apoptosis ratio is less than or equal to 10%, the cell autophagy ratio is less than 5%, no proto-oncogene is remarkably expressed, the cell aging ratio is less than or equal to 20% and the cell multiplication time is less than 48 hours is a stable cell; if one of the indexes does not reach the standard, it is determined that cell passage is unstable, and the cell culture process is stopped. The method provided by the invention can comprehensively judge the life cycle of the cell, so that the cell is applied to the optimal economic / efficient service life, the cell state can be effectively and accurately judged, and the method has good reliability.

The invention relates to the technical field of biological medicine. The invention aims to provide an application of Mn3O4 nanoparticles in relieving intervertebral disc degeneration. The manganous-manganic oxide nanoparticles Mn3O4NPs are added into an interventional drug for treating intervertebral disc degeneration as a nano-enzyme. According to the invention, Mn3O4 nanoparticles are used as a nano active catalytic enzyme, have multienzyme catalytic activity similar to superoxide dismutase, catalase and glutathione peroxidase, can effectively remove reactive oxygen species (ROS) and regulate ECM metabolism, significantly reduce cell apoptosis, delay cell senescence, inhibit inflammation and enhance autophagy, and can be used for preparing a medicine for treating hyperplasia. Therefore, annulus fibrosus AF repair in intervertebral disc degeneration treatment is promoted. The Mn3O4 nano particles have good biocompatibility, metabolites of the Mn3O4 nano particles can be used for other physiological processes, and no toxic or side effect is generated in in-vivo circulation. Meanwhile, the enzyme is stable in low-oxygen and low-pH adverse microenvironment of the intervertebral disc, and has in-vivo stability which is not possessed by other active catalytic enzymes.

The invention provides a composition for delaying senescence by activating telomerase activity. The composition is prepared from the following raw materials in parts by weight: 12.5 parts of wheat germ concentrated powder, 0.25 to 5 parts of ergothioneine, 0.25 to 5 parts of pyrroloquinoline quinone disodium salt and 0.25 to 5 parts of alpha-ketoglutaric acid. The invention further provides a preparation method and application of the composition for delaying senescence by activating telomerase activity. Through the synergistic effect of the ergothioneine and the pyrroloquinoline quinone disodium salt, the telomerase activity can be activated, the telomerase expression can be improved, the telomere length can be maintained, and the effect of delaying senescence can be achieved, so that the functional metabolism of a human body can be promoted, and the health state of the body can be improved; through the synergistic effect of the wheat germ concentrated powder and alpha-ketoglutaric acid, cell aging can be delayed; through the multi-target synergistic effect of'telomerase activation + mitochondrial repair + autophagy enhancement ', the anti-aging effect is delayed, the metabolic capability is high, the anti-aging effect is good, and safety and high efficiency are achieved; belongs to the technical field of food and health care product processing.

The invention relates to the cross technical field of biological medicine and image processing, and discloses a mesenchymal stem cell aging detection method based on image processing. The method comprises the following steps: automatically collecting bright field and multi-channel fluorescence images in an integrated cell culture monitoring device; after background correction and illumination homogenization, segmenting the cells by using a U-Net network and extracting single cell contours; calculating characteristics such as cell area, roundness, cytoplasmic ratio, nuclear form irregularity index, lysosome fluorescence intensity mean value and distribution entropy; and inputting a gradient boosting decision tree model to judge the unicellular aging state, and evaluating population aging with a 20% positive rate threshold. According to the invention, label-free, non-invasive and high-flux accurate detection is realized, and the method is superior to manual interpretation.

The invention belongs to the technical field of biology, and particularly relates to application of ginsenoside Rh1 in preparation of a product for relieving skin photoaging. It is found for the first time that the rare ginsenoside Rh1 efficiently removes ROS caused by UV and inhibits cell aging by activating a Sirt3 / Nrf2 / HO-1 pathway, and a brand new strategy is provided for developing a natural anti-photoaging preparation with repair and protection functions.

The invention discloses an application of a circRNA molecule as a diagnostic marker in a cell senescence diagnostic kit and an application of the circRNA molecule as a cell senescence treatment target. According to the application disclosed by the invention, the circHERC1 is found to be low in expression in natural aging and aging-inducing cell models, and the circHERC1 is over-expressed in endothelial cells, so that the multiplication capacity of the endothelial cells can be improved, the telomere length of the cells can be effectively prolonged, telomerase can be activated, the positive rate of beta-galactosidase of the cells can be reduced, and the expression quantity of aging-related proteins can be reduced.

The invention discloses a ganoderma lucidum lipid exosome containing a liver protection component and a preparation method and application thereof.The preparation method comprises the steps that the liver protection component is wrapped with a coarsely extracted ganoderma lucidum exosome, then the liver protection component is wrapped with lipidosome, and therefore a lipidosome-exosome-liver protection component three-layer structure is formed from outside to inside; in the three-layer structure, the outer layer liposome can protect the storage of the liver protection component and can improve the transmembrane transport efficiency, the middle layer exosome serves as a carrier and can reach a designated position to be released, and the inner layer contains the liver protection component and can improve the transmembrane transport efficiency. Cell cycle arrest can be prevented, antioxidant enzyme activity can be improved, cell membranes can be protected from oxidative damage, and redox steady state can be maintained to relieve cell aging. The prepared ganoderma lucidum lipid exosome containing the liver protection component realizes slow release synergy through a three-layer structure, and the action time of the active component is remarkably prolonged.

The invention relates to the technical field of amino acid compositions, and discloses a compound amino acid composition for enhancing telomerase activity, which comprises the following components (by weight): 20-25 parts of L-arginine; 15 to 20 parts of L-lysine; 25 to 30 parts of L-glutamine; 10 to 15 parts of L-serine; 5 to 8 parts of L-glycine; 3 to 5 parts of L-proline; 2 to 4 parts of N-acetyl-L-cysteine; 1 to 2 parts of L-tryptophan; 1 to 2 parts of beta-alanine; 2 to 3 parts of L-carnitine; 1-2 parts of taurine; through the synergistic effect of multiple mechanisms, the compound amino acid composition not only significantly enhances the telomerase activity (up to 195%), but also can maintain the telomere length, inhibit cell aging and provide antioxidant protection. The comprehensive regulation effect is derived from a unique metabolic network formed among the components, and mutual promotion and regulation are realized. Experimental results show that the long telomere length (8.3 kb vs control group 7.1 kb) can be maintained in 30-day long-term culture.

The invention relates to the technical field of biological processing, in particular to a production process and application of bovine bone collagen peptide for preventing osteoporosis and delaying cell senescence, and the production process comprises the following steps: placing yak bone into a bone placing cylinder, and plugging a rubber plug; a first motor and a second motor are started to drive a shifting block and a pushing block to quickly move from one end of a bone placing cylinder to the other end of the bone placing cylinder in a reciprocating manner, so that a plurality of bone striking groups inserted outside the bone placing cylinder are triggered to be compressed, rebounded and released, yak bones in the bone placing cylinder are struck, and concave holes and cracks are formed in the bone surface; and then the servo motor is started to drive the bone unloading belt to unload the yak bones, and the yak bones fall into the bone crushing machine body to be crushed. The spring steel conical head is driven by the cambered surface and planar force storage group to pre-split yak bones, directional impact on the surfaces of the yak bones is completed, continuous compression and release are achieved by linkage of the two-way lead screw and the shifting block and the pushing block, full-circumferential dead-corner-free impact on the yak bones is completed, the effect of increasing the crack coverage rate is achieved, the extrusion force of a subsequent bone crusher is reduced, and the production efficiency is improved. The service life of the double-tooth roller is prolonged.

The invention relates to the technical field of battery cell capacity fault prediction, and particularly discloses a battery fault prediction method and system based on a hierarchical health assessment model, and the method comprises the steps: obtaining an operation historical data set and an initial capacity; performing aging stage division on the operation historical data; constructing a three-level prediction model; outputting a capacity prediction result set; constructing a dynamic residual sequence in combination with the stage sensing information and the long-short-term memory network; and outputting an alarm result. Compared with the prior art that the difference of the aging stages of the battery cell is ignored, and particularly, the technical problems that the model prediction error is large and early warning is not timely under the condition that the battery cell enters the later stage of nonlinear capacity degradation are solved. The prediction adaptability and sensitivity in each stage are effectively improved, and the accuracy and timeliness of battery cell fault early warning are enhanced.

The invention provides a bifidobacterium animalis subspecies HX-BA21, and the preservation number of the bifidobacterium animalis subspecies HX-BA21 is CGMCC (China General Microbiological Culture Collection Center) NO.32958. The invention discloses bifidobacterium animalis subspecies HX-BA21 containing exosome metabiotics and an exosome-containing fermentation compound obtained by fermenting gastrodia elata through the HX-BA21. The fermentation compound comprises a probiotic protective layer (a core layer), an exosome delivery layer (a shell layer) and a prebiotics sustained release layer (a cocoon layer) from inside to outside. The probiotic protection layer comprises a sodium alginate-milk fat globule membrane compound and animal bifidobacterium subsp.animalis; the exosome delivery layer comprises a gelatin-chitosan compound and a gastrodia elata and probiotic mixed exosome; the prebiotic sustained-release layer comprises an octenyl succinic anhydride starch / polyvinyl alcohol compound and inulin. The compound can start cell repair by improving the cell migration rate, up-regulate DNA helicase and telomere related gene expression, delay premature senescence and delay cell senescence caused by D-galactose by adjusting the oxidative stress level.

The invention discloses an anti-aging composition and application thereof. According to the anti-aging composition disclosed by the invention, the anti-aging composition is prepared by compounding dimethyl silanol hyaluronate, radix pseudostellariae cyclic peptide B and a medicago sativa extract. The medicago sativa extract disclosed by the invention is obtained by adopting a pulsed ultrasound-assisted subcritical extraction technology, and can play a better anti-aging effect. Experiments verify that the composition can significantly increase the content of retinoic acid binding receptor RAR alpha and RXR gamma proteins and type I collagen, inhibit cell aging and achieve an anti-aging effect similar to that of retinol, but compared with retinol, the composition is mild and non-irritant, can significantly improve the preservative efficacy of a product when being used in skin care products, and can reduce the dosage of preservatives.

The invention provides a new energy automobile battery rapid detection method and device, and the method comprises the steps: extracting the discrete characteristics of the battery capacity in a to-be-detected battery pack from electrical parameter data, determining the charge state difference of each single battery in a charging process, and extracting the temperature rise characteristics of each single battery from temperature data; determining a monomer aging index of the corresponding battery monomer according to the charge state difference and the temperature rise characteristic of each battery monomer, and further determining the overall aging characteristic of the battery pack to be tested; determining the relative aging gradient of each battery monomer based on the charging and discharging duration of the battery pack to be detected and all monomer aging indexes; and determining a health state detection value of the to-be-detected battery pack through all the relative aging gradients and the overall aging characteristics, and calibrating the health state of the to-be-detected battery pack based on the health state detection value. By adopting the scheme of the invention, the battery pack can be rapidly detected based on the aging of the monomers in the battery pack and the consistency in the battery pack.

Machine learning techniques of characterizing senescence in an immobilized patient cell are disclosed. The techniques include: obtaining a patient cell image; passing the patient cell image to a trained machine learning system, where the trained machine learning system is trained using a training corpus that includes a plurality of training cell images, where the plurality of training cell images include a plurality of images of normal cells and a plurality of images of cells with senescence; obtaining an output from the trained machine learning model in response to the passing, where the output is indicative of a senescence property of the patient cell, and where the senescence property is non-binary; and providing an indication of the senescence property of the patient cell based on the output.

The present application relates to the technical field of battery safety, and particularly relates to a battery safety residual pressure evaluation method and device, electronic equipment and a storage medium, wherein the method comprises: obtaining reference data and a state of a battery cell to be evaluated; constructing a first optimization factor by using the difference between the state of the battery cell to be evaluated and the internal impedance of a brand-new battery cell in the reference data; calculating a second optimization factor by using the change acceleration characteristics of a reference temperature curve and the reference gas generation rate at the current time based on the first optimization factor; constructing a final quality source curve based on the first optimization factor and the second optimization factor; and performing safety evaluation on the battery cell to be evaluated by using the final quality source curve to obtain an evaluation result. Through two-step correction of "pre-explosion" and "dynamic instability pulse", the earlier, more intense and intermittent gas generation behavior caused by cell aging is truly mapped into the CFD model, thereby significantly improving the simulation accuracy and avoiding systematic underestimation of the pressure peak value.

The invention discloses a preparation method of an anti-aging body-building nutritional product. The preparation method comprises the following steps: step 1, weighing raw materials in parts by weight; 2, pretreating the tea polyphenol and the tea micromolecules; 3, mixing the pretreated tea polyphenol and tea micromolecules with other raw materials; step 4, adding maltodextrin and erythritol into the mixed solution, and stirring and mixing; and step 5, removing agglomerated particles, and tabletting or filling into capsules. According to the present invention, by adding the collagen peptide, the oxidation resistance of the product can be increased, the oxidative damage after exercise can be effectively relieved, the cell aging can be delayed, the traditional sucrose is cancelled, the maltodextrin and the erythritol are adopted to replace the sucrose, and the product is suitable for the long-term consumption of the body-building crowd; the preparation method solves the problems of easy oxidation and activity loss of core components in a traditional preparation method, so that substances such as tea polyphenols and tea micromolecules are more easily absorbed and utilized by human bodies.

The invention discloses an oral hard capsule health-care product with an anti-aging function as well as a preparation method and application of the oral hard capsule health-care product. The health-care product is mainly prepared from eight active ingredients including EGT (Ergothioneine), Ca-AKG (alpha-ketoglutarate), PQQ (pyrroloquinoline quinone), fisetin, salidroside, urolithin A, spermidine and an apple stem cell extract. Through scientific compatibility of the various components, the composition disclosed by the invention can comprehensively intervene in a cell aging process through a synergistic effect of a plurality of pathways of enhancing the cell antioxidant capacity, improving the mitochondrial function, activating cell autophagy, promoting cell regeneration and repair and the like. The invention also provides a preferred content range of the health-care product, a preparation method of the health-care product and application of the health-care product in preparation of health-care foods for delaying senescence, improving cell viability or improving mitochondrial functions. The product provided by the invention has the characteristics of multiple targets and synergistic interaction, and provides a new effective scheme for anti-aging intervention.

The invention discloses a medicinal and edible composition formula capable of resisting aging and improving sexual function. The invention belongs to the technical field of anti-aging. The medicinal and edible composition formula disclosed by the invention has remarkable anti-aging and sexual function improving effects; cell aging can be effectively delayed, the metabolic capability of a body is enhanced, and an endocrine system is regulated, so that a young state and a good sexual function are kept; according to the formula, natural medicinal and edible food materials are adopted, so that the safety is extremely high, and the side effect is extremely small; the formula is beneficial to improving the psychological state and life quality, enhancing the immunity of the body, improving the resistance of the body and reducing the occurrence of diseases.

The invention discloses a method for inducing fibroblasts to be reprogrammed into mesenchymal matrix-like cells (MLCs) by using small chemical molecules and application of the mesenchymal matrix-like cells (MLCs) in the anti-aging field. The invention discloses a set of specific chemical small molecule composition and an induction scheme thereof, which can be used for efficiently reprogramming fibroblasts from somatic cells into MLCs. Furthermore, the huge potential of the MLCs obtained by the method in anti-aging research is deeply explored, and the effect of the MLCs is proved to be obviously superior to that of umbilical cord mesenchymal stem cells in the aspects of reversing cell aging phenotypes and recovering tissue functions. The invention not only provides a new stem cell source strategy for avoiding the ethical and safety problems of the traditional reprogramming technology, but also lays a solid cell foundation for developing a novel, safe and effective anti-aging therapy based on cell transplantation or secreted factors.

The invention relates to the technical field of rice vinegar, in particular to a preparation method of compound flavor nutritional rice vinegar, which comprises the following steps: step 1, preparing materials; step 2, pretreatment; step 3, pre-fermentation; step 4, medium-term fermentation; step 5, acetic fermentation; the rice vinegar prepared by the preparation method disclosed by the invention contains more antioxidant substances including vitamin C, vitamin E and the like, and free radicals in a body can be removed through the excellent antioxidant performance of the antioxidant substances, so that cell aging can be prevented; the organic acid contained in the prepared rice vinegar can be beneficial for the body to absorb sugar, so that the sugar content in the blood can be finely reduced, the blood sugar concentration can be reduced, and diabetes can be prevented.

The application discloses a lithium battery protection plate cell voltage equalization and protection threshold adjustment method, and relates to the technical field of lithium battery protection plates.The application synchronously completes online estimation of a state of charge SOC and a state of health SOH of a cell and analysis of cell dispersion by collecting electrical parameters and working condition data of each single cell of a battery pack in real time;based on charging, discharging, and standing full working condition division, differential dynamic equalization control is executed, and cell equalization state, SOH dispersion, real-time working condition, and temperature data are linked to adaptively and dynamically adjust overcharge, overdischarge, overcurrent, and other protection thresholds;finally, through a whole-process closed-loop control of collection-estimation-equalization-threshold adjustment-closed-loop verification, the problems that cell equalization and protection threshold control are independent of each other and cannot adapt to cell aging and consistency differences in the prior art are avoided, protection mis-triggering and cell over-stress damage are avoided, and the cycle service life and operation safety of a lithium battery pack are significantly prolonged.

The invention belongs to the field of food science and engineering, and discloses a preparation method of an active medicinal and edible composite nutrient AMCN with an anti-aging effect, and the preparation method comprises the following steps: putting a ganoderma lucidum monoclonal strain into a liquid culture medium for controllable culture to obtain supernate with beta-glucosidase activity of over 100mU / mL; and adding rhizoma polygonati powder, lucid ganoderma powder and mulberry leaf powder, carrying out enzymatic biotransformation, carrying out lactobacillus plantarum fermentation, adding a freeze-drying protective additive, carrying out freeze-drying, grinding to obtain the active medicinal and edible composite nutrient AMCN, and storing the finished product in a dry and dark place. The active medicinal and edible composite nutrient can be used for preparing anti-aging functional foods or nutritional health-care products. A D-galactose induced zebrafish aging model verifies that the active medicinal and edible composite nutrient prepared by the application plays an anti-aging role by relieving oxidative stress, inhibiting cell aging and maintaining telomere stability, can reduce beta-galactosidase activity and intracellular ROS (reactive oxygen species) level, improves telomerase protein concentration, and has a good anti-aging effect. And the telomere length can be effectively recovered by medium and high concentrations.

The curcumin-coupled copper-gold bimetallic nanoszyme AuCu-Cur prepared by the application can remove ROS and relieve oxidative stress, prevent further damage to DNA, and introduce human umbilical cord mesenchymal stem cell-derived extracellular vesicles (hUCMSC-EVs) to improve the bioavailability. In the system, curcumin can repair damaged DNA by promoting the expression of NEIL3. In addition, the vesicles themselves are rich in various tissue repair-related proteins and RNAs, which can promote DNA replication of notochordal cells (NPCs), reverse the aging state and stimulate extracellular matrix synthesis. In the IVDD model induced by rat tail vertebra puncture, we confirmed that the material has strong antioxidant and DNA repair capacity and can effectively delay cell aging, thereby relieving the progression of IVDD. Compared with existing intervertebral disc biomaterial treatments, the present scheme can realize continuous DNA repair, which is an active, multi-target repair program that not only interrupts the vicious cycle of intervertebral disc degeneration but also promotes tissue transformation to regeneration.

The invention provides an SOC-OCV model construction and test system and method under multi-temperature static charging and discharging, and relates to the technical field of battery state-of-charge estimation, and the method comprises the steps: obtaining the operation data of a single cell in the static charging and discharging process at different temperatures, and carrying out the preprocessing of the operation data; calculating a dynamic SOC based on an ampere-hour integral method by using the preprocessed operation data, eliminating an integral error through standing OCV calibration, fitting SOC-OCV data at each temperature by using a fourth-order polynomial based on a standing SOC-OCV data pair, and constructing a multi-temperature OCV model; setting a target temperature, calculating an OCV difference value between the target temperature and a reference temperature, realizing SOC rapid correction based on the reference temperature, and updating OCV model parameters according to the cell aging data; and testing the multi-temperature OCV model at each temperature, calculating fitting OCV of SOC points, outputting SOC and OCV predicted values, and storing test data. According to the invention, accurate estimation of the state of charge (SOC) of the battery in different temperature environments is realized.

The invention relates to hair-blacking essence and a preparation method thereof. Screening a symbiotic bacterium metabolism extract with small molecule peptide metabolism regulation capability; by combining low-concentration azelaic acid and zinc lactate components, scalp grease oxidation and abnormal proliferation of propionibacterium are inhibited; the expression of IL-10 anti-inflammatory factors is induced, the expression of TNF-alpha and IL-6 is reduced, and the hair follicle microinflammation state is reduced; a Wnt / beta-catenin pathway activator is used for inducing the melanin stem cells to be in a static state; the FGF-2 and the EGF are combined in a micro-dose manner to promote the amplification into precursor cells; mITF regulation peptides and a small amount of trace copper ions are supplemented to induce directional differentiation into mature melanocytes; a microcapsule carrier system with a three-layer structure; the penetration efficiency is improved; antioxidative peptide rich in reduced glutathione, NAD and a promoting factor are used, so that melanocytes are protected from oxidative damage; a coenzyme Q10 type mitochondrial homeostasis regulator is introduced, so that the energy metabolism is improved, the cell aging is delayed, the targeting property is strong, and the effect is accurate.

The invention provides an application of an ELABELA derived peptide in preparation of a medicine for treating renal fibrosis. The ELABELA derived peptide is an ELABELA polypeptide of which the C terminal is amidated. Through screening, it is found that the amidated Elabela derived peptide has the effect of inhibiting renal fibrosis on the cellular level and the animal level, endoplasmic reticulum stress and cell aging in the renal fibrosis development process can be relieved, the amidated Elabela derived peptide can be used for preparing drugs for treating or relieving renal fibrosis diseases, and the amidated Elabela derived peptide has a large clinical transformation application prospect.

The invention discloses an NK cell amplification culture solution and application thereof, the amplification culture solution comprises a basic culture medium, and the basic culture medium comprises polysaccharide with the final concentration of 1-500 [mu] g / mL, IL-2 with the final concentration of 50-2000 U / mL, platelet lysate with the final concentration of 0.5-5% v / v, a concentrated extract of macaque rhizomes with the final concentration of 0.1-1% v / v and a concentrated extract of gentian rhizomes with the final concentration of 0.1-1% v / v. According to the NK cell amplification culture solution, the concentrated extract components of the macaque rhizomes and the gentian rhizomes can maintain the activity of NK cells, slow down cell aging, promote secretion of cell factors and maintain long-period (24 days or more) cell proliferation; meanwhile, the NK cell culture process is simple, coating is not needed, and operation is convenient.

The invention discloses an application of an engineered mRNA (messenger Ribonucleic Acid) medicine for coding COL3A1 protein in resisting skin photoaging. According to the method, accurate rational design and optimization are carried out on the 5'untranslated region (UTR) of the hCOL3A1 mRNA, so that the translation efficiency and the protein yield of the hCOL3A1 mRNA are greatly improved, the expression level of the hCOL3A1 target protein far beyond the conventional sequence in vivo and in vitro is realized, and the type III collagen is efficiently and endogenously synthesized in skin cells. Experimental results show that the engineered mRNA can effectively reduce the skin oxidative stress level, inhibit cell aging and promote endogenous synthesis and deposition of corium layer collagen, so that the barrier function of photoaged skin is remarkably improved, the dermis thickness is increased, the tissue structure is repaired, and systemic toxicity or immune side effects are not observed. The invention provides a novel strategy with high efficiency and safety for resisting skin photoaging.