127 results about "Cell aging" patented technology

The invention provides a strain of lactobacillus plantarum YS-AG23 with high antioxidant activity, and a rhizoma polygonati and radix angelicae sinensis fermented product obtained by fermenting rhizoma polygonati and radix angelicae sinensis with the strain of lactobacillus plantarum YS-AG23. In a D-galactose induced premature ovarian failure mouse model, the fermentation product can regulate up the levels of antioxidant and stress protection genes SOD2 and HO-1 and an anti-aging gene SIRT1 by regulating down the expression of a key gene AGER of an inflammation and oxidative stress pathway, so that the oxidative stress and inflammatory response of model mouse ovarian tissues are remarkably reduced; meanwhile, the serum hormone level can be specifically adjusted, the serum estradiol (E2) level can be increased, the follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels can be reduced to be close to the normal range, the expression of anti-mullerian hormone (AMH) and hormone regulatory gene LHR of ovarian reserve markers can be up-regulated, the proliferative activity and estrogen secretion function of follicular granulosa cells can be improved, and the effect of preventing ovarian reserve is achieved. The wet weight of atrophic uterus and ovary of the model mouse is remarkably increased, uterine and ovary atrophy is effectively reversed, and the ovarian reserve function of the D-galactose induced premature ovarian failure model mouse is recovered. Besides, in an H2O2-induced human dermal fibroblast aging model, the yeast can improve the activity of aging cells in a concentration-dependent manner and reduce the level of reactive oxygen species (ROS) in the cells; in addition, by up-regulating the expression of a collagen synthesis key gene COL1A1 and down-regulating the expression of a cell senescence marker gene p16, skin cell senescence caused by oxidative stress can be effectively improved. In conclusion, the polygonatum sibiricum and angelica sinensis leavening can synchronously improve premature ovarian failure and skin cell senescence caused by oxidative stress by accurately regulating and controlling oxidative stress, inflammatory response and tissue function related genes, and has wide application potential.

The invention provides a composition for delaying senescence by activating telomerase activity. The composition is prepared from the following raw materials in parts by weight: 12.5 parts of wheat germ concentrated powder, 0.25 to 5 parts of ergothioneine, 0.25 to 5 parts of pyrroloquinoline quinone disodium salt and 0.25 to 5 parts of alpha-ketoglutaric acid. The invention further provides a preparation method and application of the composition for delaying senescence by activating telomerase activity. Through the synergistic effect of the ergothioneine and the pyrroloquinoline quinone disodium salt, the telomerase activity can be activated, the telomerase expression can be improved, the telomere length can be maintained, and the effect of delaying senescence can be achieved, so that the functional metabolism of a human body can be promoted, and the health state of the body can be improved; through the synergistic effect of the wheat germ concentrated powder and alpha-ketoglutaric acid, cell aging can be delayed; through the multi-target synergistic effect of'telomerase activation + mitochondrial repair + autophagy enhancement ', the anti-aging effect is delayed, the metabolic capability is high, the anti-aging effect is good, and safety and high efficiency are achieved; belongs to the technical field of food and health care product processing.

The invention relates to the cross technical field of biological medicine and image processing, and discloses a mesenchymal stem cell aging detection method based on image processing. The method comprises the following steps: automatically collecting bright field and multi-channel fluorescence images in an integrated cell culture monitoring device; after background correction and illumination homogenization, segmenting the cells by using a U-Net network and extracting single cell contours; calculating characteristics such as cell area, roundness, cytoplasmic ratio, nuclear form irregularity index, lysosome fluorescence intensity mean value and distribution entropy; and inputting a gradient boosting decision tree model to judge the unicellular aging state, and evaluating population aging with a 20% positive rate threshold. According to the invention, label-free, non-invasive and high-flux accurate detection is realized, and the method is superior to manual interpretation.

The invention belongs to the technical field of biology, and particularly relates to application of ginsenoside Rh1 in preparation of a product for relieving skin photoaging. It is found for the first time that the rare ginsenoside Rh1 efficiently removes ROS caused by UV and inhibits cell aging by activating a Sirt3 / Nrf2 / HO-1 pathway, and a brand new strategy is provided for developing a natural anti-photoaging preparation with repair and protection functions.

The invention discloses a ganoderma lucidum lipid exosome containing a liver protection component and a preparation method and application thereof.The preparation method comprises the steps that the liver protection component is wrapped with a coarsely extracted ganoderma lucidum exosome, then the liver protection component is wrapped with lipidosome, and therefore a lipidosome-exosome-liver protection component three-layer structure is formed from outside to inside; in the three-layer structure, the outer layer liposome can protect the storage of the liver protection component and can improve the transmembrane transport efficiency, the middle layer exosome serves as a carrier and can reach a designated position to be released, and the inner layer contains the liver protection component and can improve the transmembrane transport efficiency. Cell cycle arrest can be prevented, antioxidant enzyme activity can be improved, cell membranes can be protected from oxidative damage, and redox steady state can be maintained to relieve cell aging. The prepared ganoderma lucidum lipid exosome containing the liver protection component realizes slow release synergy through a three-layer structure, and the action time of the active component is remarkably prolonged.

The invention relates to the technical field of biological processing, in particular to a production process and application of bovine bone collagen peptide for preventing osteoporosis and delaying cell senescence, and the production process comprises the following steps: placing yak bone into a bone placing cylinder, and plugging a rubber plug; a first motor and a second motor are started to drive a shifting block and a pushing block to quickly move from one end of a bone placing cylinder to the other end of the bone placing cylinder in a reciprocating manner, so that a plurality of bone striking groups inserted outside the bone placing cylinder are triggered to be compressed, rebounded and released, yak bones in the bone placing cylinder are struck, and concave holes and cracks are formed in the bone surface; and then the servo motor is started to drive the bone unloading belt to unload the yak bones, and the yak bones fall into the bone crushing machine body to be crushed. The spring steel conical head is driven by the cambered surface and planar force storage group to pre-split yak bones, directional impact on the surfaces of the yak bones is completed, continuous compression and release are achieved by linkage of the two-way lead screw and the shifting block and the pushing block, full-circumferential dead-corner-free impact on the yak bones is completed, the effect of increasing the crack coverage rate is achieved, the extrusion force of a subsequent bone crusher is reduced, and the production efficiency is improved. The service life of the double-tooth roller is prolonged.

The invention relates to the technical field of battery cell capacity fault prediction, and particularly discloses a battery fault prediction method and system based on a hierarchical health assessment model, and the method comprises the steps: obtaining an operation historical data set and an initial capacity; performing aging stage division on the operation historical data; constructing a three-level prediction model; outputting a capacity prediction result set; constructing a dynamic residual sequence in combination with the stage sensing information and the long-short-term memory network; and outputting an alarm result. Compared with the prior art that the difference of the aging stages of the battery cell is ignored, and particularly, the technical problems that the model prediction error is large and early warning is not timely under the condition that the battery cell enters the later stage of nonlinear capacity degradation are solved. The prediction adaptability and sensitivity in each stage are effectively improved, and the accuracy and timeliness of battery cell fault early warning are enhanced.

The invention provides a bifidobacterium animalis subspecies HX-BA21, and the preservation number of the bifidobacterium animalis subspecies HX-BA21 is CGMCC (China General Microbiological Culture Collection Center) NO.32958. The invention discloses bifidobacterium animalis subspecies HX-BA21 containing exosome metabiotics and an exosome-containing fermentation compound obtained by fermenting gastrodia elata through the HX-BA21. The fermentation compound comprises a probiotic protective layer (a core layer), an exosome delivery layer (a shell layer) and a prebiotics sustained release layer (a cocoon layer) from inside to outside. The probiotic protection layer comprises a sodium alginate-milk fat globule membrane compound and animal bifidobacterium subsp.animalis; the exosome delivery layer comprises a gelatin-chitosan compound and a gastrodia elata and probiotic mixed exosome; the prebiotic sustained-release layer comprises an octenyl succinic anhydride starch / polyvinyl alcohol compound and inulin. The compound can start cell repair by improving the cell migration rate, up-regulate DNA helicase and telomere related gene expression, delay premature senescence and delay cell senescence caused by D-galactose by adjusting the oxidative stress level.

The invention provides a new energy automobile battery rapid detection method and device, and the method comprises the steps: extracting the discrete characteristics of the battery capacity in a to-be-detected battery pack from electrical parameter data, determining the charge state difference of each single battery in a charging process, and extracting the temperature rise characteristics of each single battery from temperature data; determining a monomer aging index of the corresponding battery monomer according to the charge state difference and the temperature rise characteristic of each battery monomer, and further determining the overall aging characteristic of the battery pack to be tested; determining the relative aging gradient of each battery monomer based on the charging and discharging duration of the battery pack to be detected and all monomer aging indexes; and determining a health state detection value of the to-be-detected battery pack through all the relative aging gradients and the overall aging characteristics, and calibrating the health state of the to-be-detected battery pack based on the health state detection value. By adopting the scheme of the invention, the battery pack can be rapidly detected based on the aging of the monomers in the battery pack and the consistency in the battery pack.

The present application relates to the technical field of battery safety, and particularly relates to a battery safety residual pressure evaluation method and device, electronic equipment and a storage medium, wherein the method comprises: obtaining reference data and a state of a battery cell to be evaluated; constructing a first optimization factor by using the difference between the state of the battery cell to be evaluated and the internal impedance of a brand-new battery cell in the reference data; calculating a second optimization factor by using the change acceleration characteristics of a reference temperature curve and the reference gas generation rate at the current time based on the first optimization factor; constructing a final quality source curve based on the first optimization factor and the second optimization factor; and performing safety evaluation on the battery cell to be evaluated by using the final quality source curve to obtain an evaluation result. Through two-step correction of "pre-explosion" and "dynamic instability pulse", the earlier, more intense and intermittent gas generation behavior caused by cell aging is truly mapped into the CFD model, thereby significantly improving the simulation accuracy and avoiding systematic underestimation of the pressure peak value.

The invention discloses a preparation method of an anti-aging body-building nutritional product. The preparation method comprises the following steps: step 1, weighing raw materials in parts by weight; 2, pretreating the tea polyphenol and the tea micromolecules; 3, mixing the pretreated tea polyphenol and tea micromolecules with other raw materials; step 4, adding maltodextrin and erythritol into the mixed solution, and stirring and mixing; and step 5, removing agglomerated particles, and tabletting or filling into capsules. According to the present invention, by adding the collagen peptide, the oxidation resistance of the product can be increased, the oxidative damage after exercise can be effectively relieved, the cell aging can be delayed, the traditional sucrose is cancelled, the maltodextrin and the erythritol are adopted to replace the sucrose, and the product is suitable for the long-term consumption of the body-building crowd; the preparation method solves the problems of easy oxidation and activity loss of core components in a traditional preparation method, so that substances such as tea polyphenols and tea micromolecules are more easily absorbed and utilized by human bodies.

The invention discloses an oral hard capsule health-care product with an anti-aging function as well as a preparation method and application of the oral hard capsule health-care product. The health-care product is mainly prepared from eight active ingredients including EGT (Ergothioneine), Ca-AKG (alpha-ketoglutarate), PQQ (pyrroloquinoline quinone), fisetin, salidroside, urolithin A, spermidine and an apple stem cell extract. Through scientific compatibility of the various components, the composition disclosed by the invention can comprehensively intervene in a cell aging process through a synergistic effect of a plurality of pathways of enhancing the cell antioxidant capacity, improving the mitochondrial function, activating cell autophagy, promoting cell regeneration and repair and the like. The invention also provides a preferred content range of the health-care product, a preparation method of the health-care product and application of the health-care product in preparation of health-care foods for delaying senescence, improving cell viability or improving mitochondrial functions. The product provided by the invention has the characteristics of multiple targets and synergistic interaction, and provides a new effective scheme for anti-aging intervention.

The invention discloses a method for inducing fibroblasts to be reprogrammed into mesenchymal matrix-like cells (MLCs) by using small chemical molecules and application of the mesenchymal matrix-like cells (MLCs) in the anti-aging field. The invention discloses a set of specific chemical small molecule composition and an induction scheme thereof, which can be used for efficiently reprogramming fibroblasts from somatic cells into MLCs. Furthermore, the huge potential of the MLCs obtained by the method in anti-aging research is deeply explored, and the effect of the MLCs is proved to be obviously superior to that of umbilical cord mesenchymal stem cells in the aspects of reversing cell aging phenotypes and recovering tissue functions. The invention not only provides a new stem cell source strategy for avoiding the ethical and safety problems of the traditional reprogramming technology, but also lays a solid cell foundation for developing a novel, safe and effective anti-aging therapy based on cell transplantation or secreted factors.

The application discloses a lithium battery protection plate cell voltage equalization and protection threshold adjustment method, and relates to the technical field of lithium battery protection plates.The application synchronously completes online estimation of a state of charge SOC and a state of health SOH of a cell and analysis of cell dispersion by collecting electrical parameters and working condition data of each single cell of a battery pack in real time;based on charging, discharging, and standing full working condition division, differential dynamic equalization control is executed, and cell equalization state, SOH dispersion, real-time working condition, and temperature data are linked to adaptively and dynamically adjust overcharge, overdischarge, overcurrent, and other protection thresholds;finally, through a whole-process closed-loop control of collection-estimation-equalization-threshold adjustment-closed-loop verification, the problems that cell equalization and protection threshold control are independent of each other and cannot adapt to cell aging and consistency differences in the prior art are avoided, protection mis-triggering and cell over-stress damage are avoided, and the cycle service life and operation safety of a lithium battery pack are significantly prolonged.

The invention belongs to the field of food science and engineering, and discloses a preparation method of an active medicinal and edible composite nutrient AMCN with an anti-aging effect, and the preparation method comprises the following steps: putting a ganoderma lucidum monoclonal strain into a liquid culture medium for controllable culture to obtain supernate with beta-glucosidase activity of over 100mU / mL; and adding rhizoma polygonati powder, lucid ganoderma powder and mulberry leaf powder, carrying out enzymatic biotransformation, carrying out lactobacillus plantarum fermentation, adding a freeze-drying protective additive, carrying out freeze-drying, grinding to obtain the active medicinal and edible composite nutrient AMCN, and storing the finished product in a dry and dark place. The active medicinal and edible composite nutrient can be used for preparing anti-aging functional foods or nutritional health-care products. A D-galactose induced zebrafish aging model verifies that the active medicinal and edible composite nutrient prepared by the application plays an anti-aging role by relieving oxidative stress, inhibiting cell aging and maintaining telomere stability, can reduce beta-galactosidase activity and intracellular ROS (reactive oxygen species) level, improves telomerase protein concentration, and has a good anti-aging effect. And the telomere length can be effectively recovered by medium and high concentrations.

The curcumin-coupled copper-gold bimetallic nanoszyme AuCu-Cur prepared by the application can remove ROS and relieve oxidative stress, prevent further damage to DNA, and introduce human umbilical cord mesenchymal stem cell-derived extracellular vesicles (hUCMSC-EVs) to improve the bioavailability. In the system, curcumin can repair damaged DNA by promoting the expression of NEIL3. In addition, the vesicles themselves are rich in various tissue repair-related proteins and RNAs, which can promote DNA replication of notochordal cells (NPCs), reverse the aging state and stimulate extracellular matrix synthesis. In the IVDD model induced by rat tail vertebra puncture, we confirmed that the material has strong antioxidant and DNA repair capacity and can effectively delay cell aging, thereby relieving the progression of IVDD. Compared with existing intervertebral disc biomaterial treatments, the present scheme can realize continuous DNA repair, which is an active, multi-target repair program that not only interrupts the vicious cycle of intervertebral disc degeneration but also promotes tissue transformation to regeneration.

The invention provides an SOC-OCV model construction and test system and method under multi-temperature static charging and discharging, and relates to the technical field of battery state-of-charge estimation, and the method comprises the steps: obtaining the operation data of a single cell in the static charging and discharging process at different temperatures, and carrying out the preprocessing of the operation data; calculating a dynamic SOC based on an ampere-hour integral method by using the preprocessed operation data, eliminating an integral error through standing OCV calibration, fitting SOC-OCV data at each temperature by using a fourth-order polynomial based on a standing SOC-OCV data pair, and constructing a multi-temperature OCV model; setting a target temperature, calculating an OCV difference value between the target temperature and a reference temperature, realizing SOC rapid correction based on the reference temperature, and updating OCV model parameters according to the cell aging data; and testing the multi-temperature OCV model at each temperature, calculating fitting OCV of SOC points, outputting SOC and OCV predicted values, and storing test data. According to the invention, accurate estimation of the state of charge (SOC) of the battery in different temperature environments is realized.

The invention relates to hair-blacking essence and a preparation method thereof. Screening a symbiotic bacterium metabolism extract with small molecule peptide metabolism regulation capability; by combining low-concentration azelaic acid and zinc lactate components, scalp grease oxidation and abnormal proliferation of propionibacterium are inhibited; the expression of IL-10 anti-inflammatory factors is induced, the expression of TNF-alpha and IL-6 is reduced, and the hair follicle microinflammation state is reduced; a Wnt / beta-catenin pathway activator is used for inducing the melanin stem cells to be in a static state; the FGF-2 and the EGF are combined in a micro-dose manner to promote the amplification into precursor cells; mITF regulation peptides and a small amount of trace copper ions are supplemented to induce directional differentiation into mature melanocytes; a microcapsule carrier system with a three-layer structure; the penetration efficiency is improved; antioxidative peptide rich in reduced glutathione, NAD and a promoting factor are used, so that melanocytes are protected from oxidative damage; a coenzyme Q10 type mitochondrial homeostasis regulator is introduced, so that the energy metabolism is improved, the cell aging is delayed, the targeting property is strong, and the effect is accurate.

The invention provides an application of an ELABELA derived peptide in preparation of a medicine for treating renal fibrosis. The ELABELA derived peptide is an ELABELA polypeptide of which the C terminal is amidated. Through screening, it is found that the amidated Elabela derived peptide has the effect of inhibiting renal fibrosis on the cellular level and the animal level, endoplasmic reticulum stress and cell aging in the renal fibrosis development process can be relieved, the amidated Elabela derived peptide can be used for preparing drugs for treating or relieving renal fibrosis diseases, and the amidated Elabela derived peptide has a large clinical transformation application prospect.

The invention discloses an application of an engineered mRNA (messenger Ribonucleic Acid) medicine for coding COL3A1 protein in resisting skin photoaging. According to the method, accurate rational design and optimization are carried out on the 5'untranslated region (UTR) of the hCOL3A1 mRNA, so that the translation efficiency and the protein yield of the hCOL3A1 mRNA are greatly improved, the expression level of the hCOL3A1 target protein far beyond the conventional sequence in vivo and in vitro is realized, and the type III collagen is efficiently and endogenously synthesized in skin cells. Experimental results show that the engineered mRNA can effectively reduce the skin oxidative stress level, inhibit cell aging and promote endogenous synthesis and deposition of corium layer collagen, so that the barrier function of photoaged skin is remarkably improved, the dermis thickness is increased, the tissue structure is repaired, and systemic toxicity or immune side effects are not observed. The invention provides a novel strategy with high efficiency and safety for resisting skin photoaging.

This invention provides a method for constructing a human endometrial organoid aging model, belonging to the field of biological model technology. Based on human endometrial organoids, this invention utilizes hydrogen peroxide (H2O2) stimulation to induce a human endometrial organoid aging model. This human endometrial organoid aging model clearly exhibits multiple cellular aging phenotypes, including slowed organoid growth rate, decreased cell proliferation index (Ki67), enhanced SA-β-gal activity, upregulated expression of cell cycle inhibitors P16 and P21, and significantly upregulated gene expression levels of aging-associated secretory phenotype (SASP) components. It can provide an effective tool for screening anti-aging drugs for women, high-throughput and high-content screening, personalized treatment strategy development, and mechanistic research.

The invention discloses an application of a substance for reducing the content or activity of H2AZ1 in senescence prevention. It is found that replicative aging and pathological aging of mesenchymal stem cells can be delayed by knocking out the H2AZ1 gene, and cell aging can be promoted by increasing the expression quantity of H2AZ1. The invention provides a new thought for developing gene therapy for delaying senescence, expands the selectable range of clinical gene therapy, and has a good application prospect.

The invention relates to plant source dry-state mitochondria for cell repair and aging resistance as well as a preparation method and application of the plant source dry-state mitochondria. The plant source dry-state mitochondria can effectively improve functions of mitochondria, reduce the damage degree of cells, inhibit cell aging and effectively repair skin wounds. According to the method, the mitochondria is extracted from healthy plant tissues, so that the production cost is reduced, and a harsh quarantine step is not needed. According to the present invention, the dry-state processing enables the mitochondria storage condition to be loose, the transportation and storage difficulty and the transportation and storage cost can be further reduced, the plant source mitochondria subjected to the dry-state processing has characteristics of flexible dosage form, wide auxiliary material application range, and meeting of the design requirements of various products, and the dry-state processing can further remove the nucleic acid in the extract and eliminate the inflammation of the product; in the research process, the inventor also finds that after the plant source mitochondria is subjected to dry-state processing, the mitochondria function can be more effectively improved, the cell damage degree is reduced, cell aging is inhibited, and the skin wound surface is effectively repaired.

The invention belongs to the technical field of crystal engineering, and discloses a lutein-glutamic acid eutectic crystal and a preparation method and application thereof, Cu-k alpha radiation is used, and the lutein-glutamic acid eutectic crystal has characteristic peaks at 21.75 + / -0.2 degrees, 25.95 + / -0.2 degrees, 31.34 + / -0.2 degrees and 35.96 + / -0.2 degrees in an X-ray powder diffraction pattern represented by a diffraction angle 2 theta. According to the lutein-glutamic acid eutectic crystal provided by the invention, on the premise of not changing the pharmacological activity of the lutein, the solubility and the dissolution rate of the lutein are remarkably improved, and the stability of the lutein-glutamic acid eutectic crystal under the conditions of high temperature, high humidity and strong light is greatly enhanced; in-vitro cell experiments further prove that compared with pure xanthophyll, the xanthophyll-glutamic acid eutectic has more excellent cell aging resistance, oxidation resistance and vasodilation promoting activity, and an ideal dosage form is provided for efficient application of xanthophyll in medicines and functional foods.

The invention provides an application of a component A as or used for preparing an SIRT5 inducer, the component A at least comprises one or more than two of ergothioneine, ectoine and hyaluronic acid or salt thereof, and the component A can be used for the SIRT5 inducer or used for preparing an SIRT5 expression inducer, so that cell aging can be inhibited, and the SIRT5 inducer can be used for preparing an SIRT5 expression inducer. The traditional Chinese medicine composition is used for improving natural skin aging, cardiac hypertrophy and / or hyperammonemia.

The invention discloses an automatic internal resistance and voltage detection device and method for lithium ion cell aging treatment, and belongs to the technical field of lithium battery production. The turntable is horizontally arranged and is driven by an intermittent rotating mechanism to rotate, a plurality of battery cell fixing grooves are fixed on the upper surface of the turntable, a vertically arranged probe bracket is arranged on the outer side of the turntable, the lower end of the probe bracket is fixedly connected with the rack, and the upper end of the probe bracket is fixedly connected with a cylinder body of a vertically arranged air cylinder; a piston rod of the air cylinder is fixedly connected with a test probe, and the test probe is connected with a corresponding acquisition sensing line and is correspondingly matched with the battery cell fixing groove. According to the invention, manual operation is replaced, human resource configuration is optimized, accuracy and repeatability of detection results are improved, programmed execution of detection standards is realized, the problem of inconsistent standard execution caused by human factors is avoided, cell grouping and pairing precision is improved, and cell performance consistency control is guaranteed.

The application discloses an ALKBH5-promoted cell aging mechanism and an RNA epigenetic regulation cell aging preparation, relates to the technical field of biological medicine, and aims to solve the technical problem of lack of ALKBH5-regulated cell aging mechanism in the prior art. The mechanism is that retention of ALKBH5 in the cytoplasm promotes cell aging; retention of ALKBH5 in the cytoplasm leads to m6A disorder, and further causes cell aging; aggregation of ALKBH5 in the cytoplasm causes cytoplasm retention, leads to m6A disorder and m6A hypermethylation of Cdk2, and further causes Cdk2 RNA instability, and further causes cell aging; a key mediating factor for ALKBH5 entering the nucleus is Nup62; by transfecting an RNA probe containing m6A modification into cells, it is found that m6A-labeled RNA can effectively reverse cytoplasmic aggregation of ALKBH5; and an adeno-associated virus of NLS-labeled ALKBH5 is used for supplementing ALKBH5 to the nucleus in vivo, so as to inhibit cell aging.

The application provides a composition and application thereof in preparation of a mitochondrial function regulator, and relates to the technical field of hyaluronic acid grafts. It is found for the first time in the application that N-sodium hyaluronoyl glutamate can effectively improve or restore mitochondrial function, improve cell energy metabolism, and achieve the effects of improving cell aging and / or improving cell activity. A new efficacy component is provided for developing a mitochondrial function regulator, a product for preventing and / or improving aging; further, N-sodium hyaluronoyl glutamate can break the plateau period of the action of ergothioneine, and provides a more effective scheme for further reversing mitochondrial function.

The application discloses an engineered exosome targeting human urokinase-type plasminogen activator receptor uPAR and a preparation method and application thereof. Firstly, a high-affinity anti-human uPAR single-domain antibody VHH is prepared; then, a human umbilical cord mesenchymal stem cell engineering cell line expressing a VHH-PTGFRN fusion protein is constructed, and the engineered exosome is prepared and purified by combining three-dimensional hypoxic culture, chemical induction and size-exclusion chromatography technology. The engineered exosome comprises four comparative samples, wherein the core sample Exo^(VHH / miR) can simultaneously display the anti-uPAR VHH on the membrane surface to realize targeted delivery and enrich miR-193b-3p in the chamber to realize gene silencing, and the synergistic effect of the two can efficiently remove the uPAR high-expression senescent cells and inhibit the senescence-associated secretory phenotype SASP. The engineered exosome has the advantages of standardized preparation process, high yield and high purity, strong targeting, excellent curative effect and good safety, and can be used for preparing a treatment preparation for anti-aging and aging-related diseases, and has important clinical application value.