80 results about "Redox sensitive" patented technology

The disclosure provides proteins that can be used to determine the redox status of an environment (such as the environment within a cell or subcellular compartment). These proteins are green fluorescent protein (GFP) variants (also referred to as redox sensitive GFP (rosGFP) mutants), which have been engineered to have two cysteine amino acids near the chromophore and within disulfide bonding distance of each other. Also provided are nucleic acid molecules that encode rosGFPs, vectors containing such encoding molecules, and cells transformed therewith. The disclosure further provides methods of using the rosGFPs (and encoding molecules) to analyze the redox status of an environment, such as a cell, or a subcellular compartment within a cell. In certain embodiments, both redox status and pH are analyzed concurrently.

This invention relates to an irreversible indicator for detecting oxidising agents, or in particular an oxygen indicator, comprising at least one redox-sensitive dyestuff, at least one semiconductor material and at least one electron donor. This indicator is activated by exposure to light of about 200-400 nm. The invention also relates to UV light detector.

A medium for isolating or releasing an electrostatically charged component from or into an aqueous composition. The medium has a polyelectrolyte film on at least one surface of an article wherein the polyelectrolyte film is characterized by an interpenetrating network of a predominantly positively charged polymer and a predominantly negatively charged polymer. The predominantly positively charged polymer, the predominantly negatively charged polymer or both contain (i) a pH sensitive imidazole repeat unit having a pKa between 3 and 9, or (ii) a redox sensitive repeat unit selected from the group consisting of quaternized bipyridine repeat units, coordinated metal repeat units, pyrrole repeat units, aniline repeat units, thiophene repeat units and combinations thereof having a redox potential between +1.2 volts and −1.2 volts versus a standard hydrogen electrode.

This invention relates to an indicator for detecting a photocatalyst wherein the indicator comprises at least one redox-sensitive material and either at least one electron donor or at least one electron acceptor. The invention also relates to a method for detecting a photocatalyst using an indicator. In particular, the photocatalyst may be a semiconductor such as titanium dioxide on a glass substrate.

The invention relates to a dual sensitive amphiphilic triblock copolymer containing disulfide bond and acylhydrazone bond and a preparation method and an application of the dual sensitive amphiphilic triblock copolymer. A BAB type amphiphilic triblock copolymer consists of a hydrophobic polyester block A and a hydrophilia polyethylene glycol block B, wherein the block A is polyether containing active terminal amino group and disulfide bond; the block B is benzaldehyde ester of an ethylene glycol monomethyl ether or single terminated polyoxyethylene polypropylene oxide copolymer; the block A and the block B are connected through acid sensitive acylhydrazone bond generated from reaction of active terminal amino group and aldehyde group. The dual sensitive amphiphilic triblock copolymer is good in biocompatibility and biodegradability, can be formed into nanoparticles with oxidation reduction sensitivity and acid sensitivity in a water medium in a self-assembling manner, can wrap hydrophobic medicines to form nano medicine preparations, and can promote rapid release of medicines in tumor cells due to fracture of disulfide bond and acylhydrazone bond which are sensitive to oxidation reduction in a high concentration glutathione and acid micro environment in the tumor cells.

The invention discloses a gene vector system of a redox sensitive shielding system having targeting function, its preparation and application in the field of gene therapy. The gene vector system disclosed herein comprises a redox sensitive shielding system having targeting function, a cationic high-molecular material and plasmid DNA; wherein the cationic high-molecular material and plasmid DNA form compound particles, the redox sensitive shielding system having targeting function shields the compound surface by electrostatic action, thus the toxicity of the vector can be reduced, the loaded genetic material is transferred into the cell successfully, the expression of the genetic material is realized, the transfection process is completed, the targeting of gene transfection can be raised, and simultaneously the gene transfection efficiency is raised.

The invention relates to a preparation method and application of hyaluronic acid modified by vitamin E succinate and is capable of effectively solving the problems of preparation of hyaluronic acid modified by vitamin E succinate and the application in antitumor drugs. The preparation method comprises the following steps: with alkylidene diamine or dithio alkylidene diamine as a linking arm, hyaluronic acid is covalently linked to vitamin E succinate according to the weight ratio of 1:(1-100) so as to form a nano-micelle in a water medium, wherein hyaluronic acid is low-molecular-weight hyaluronic acid with the molecular weight no less than 600 daltons and no larger than 400kd; the linking arm is the alkylidene diamine or the dithio alkylidene diamine with the amount of carbon atoms being 2-12, and a disulfide bond in the dithio alkylidene diamine has the oxidation-reduction sensitivity in vivo and is capable of releasing the antitumor drugs in a targeting mode in tumor cells. With HA as a skeleton, hyaluronic acid modified by vitamin E succinate is high in drug loading capacity and easy in releasing of the drugs, is effectively used for preparing the antitumor drugs, is capable of solving the problem of medication for treating tumors and is an innovation of the drugs for treating the tumors.

The disclosure provides proteins that can be used to determine the redox status of an environment (such as the environment within a cell or subcellular compartment). These proteins are green fluorescent protein (GFP) variants (also referred to as redox sensitive GFP (rosGFP) mutants), which have been engineering to have two cysteine amino acids near the chromophore and within disulfide bonding distance of each other. Also provided are nucleic acid molecules that encode rosGFPs, vectors containing such encoding molecules, and cells transformed therewith. The disclosure further provides methods of using the rosGFPs (and encoding molecules) to analyze the redox status of an environment, such as a cell, or a subcellular compartment within a cell. In certain embodiments, both redox status and pH are analyzed concurrently.

The invention belongs to the technical field of medicines, and relates to a redox sensitive albumin binding prodrug with a series of tumor tissue specific responses. The albumin binding prodrug is ananti-tumor drug-maleimide molecule prodrug particularly including three docetaxel-maleimide small molecule prodrugs; by utilizing a maleimide ring in a prodrug structure as a target head of plasma albumin 34-bit free sulfydryl in a binding body, so that after a drug enters into a body through intravenous injection, the drug is quickly and specifically bound with albumin to form an albumin-drug composition; therefore, the drug stability is enhanced, the circulation time of the drug in the boy is obviously prolonged, and accumulation of the drug on tumor tissues is realized by utilizing an EPR (Enhanced Permeability and Retention) effect. In addition, in the prodrug structure, by using a redox sensitive key as a binding key, quick release of the drug in tumor cells is promoted, a toxic effect of the drug on normal cells also can be alleviated, the prodrug has high selectivity, the aims of increasing an effect and reducing the toxicity of docetaxel are fulfilled, and a greater market application prospect is achieved.

The invention relates to a pH-redox double sensitive amphiphilic polymer of which the structural formula is disclosed in the specification, wherein X=15-35, Y=1-2, PEG is a polyethyleneglycol monomethyl ether chain segment, and the molecular weight is 9500-25000. The preparation method comprises the following steps: mixing raw materials, reacting in an oil bath in a nitrogen atmosphere, eluting, dissolving in dichloromethane, adding petroleum ether to precipitate under ice bath conditions, standing, centrifugating to remove solvent, dissolving in dichloromethane, flushing, carrying out centrifugal drying, and drying in an oven. By introducing the pH-sensitive monomer and redox-sensitive crosslinking agent into the polymer structure, the pH-redox double sensitive amphiphilic polymer has favorable pH sensitivity and redox sensitivity, can be used as a drug carrier material, has environment response capacity, can make a response according to the environmental change when arriving at the target spot position, and effectively implements controlled release of drugs.

The invention relates to curcumin prodrug micelle with oxidation and reduction sensitivity, a micellar monomer and a preparation method of the micellar monomer. The invention aims at providing a synthesis design method of the curcumin prodrug micelle with oxidation and reduction sensitivity. A molecular formula of the curcumin prodrug micelle monomer is as follows: MPEG-PLA-SS-Cur. According to the preparation method of the curcumin prodrug micelle monomer, raw materials for reaction comprise (methoxyl poly(ethylene glycol)-polylactic acid (MPEG-PLA), and curcumin Cur-SS-COOH modified by dithiodipropionic acid. The curcumin prodrug micelle with oxidation and reduction sensitivity, the micellar monomer and the preparation method provided by the invention have the following advantages that the micellar monomer product obtained by the preparation method has reduction responsiveness on the curcumin prodrug micelle, breaks through a conventional drug administration mode of encapsulating a drug by using a carrier, i.e., the drug is a part of the carrier; by controlling the use of inert carrier materials, the drug loading capacity, particle size and stability of a micellar system are improved significantly, and the EPR (Ethylene Propylene Rubber) effect is enhanced.

The invention discloses a multifunctional supramolecular gene delivery system based on polyethyleneimine-cyclodextrin and a preparation method thereof. A polyethyleneimine-cyclodextrin polycationic compound serves as a framework, adamantane-disulfide bond-polyethylene glycol having oxidation reduction reactivity and adamantane-polyethylene glycol-targeting peptide having targeting performance serve as modified groups, polyethyleneimine-cyclodextrin is combined with adamantane-disulfide bond-polyethylene glycol and adamantane-polyethylene glycol-targeting peptide through a self-assembling principle, and a subjective body and an objective body act to form a supramolecular gene carrier. The delivery system is multifunctional, has oxidation reduction sensitivity, specificity for targeting liver cancer and excellent gene transfection efficiency, and can carry functional genes to efficiently treat liver malignant tumor.

The invention discloses double-targeting and pH / oxidation reduction double-sensitive core cross-linking nanoparticles as well as a preparation method and application. Hydrophilic layers with folic acid targeting ligand and poly 6O-methyl acryloyl chloride-D-galactopyranose (PMApGP) are arranged on the surfaces of nanoparticles, hydrophobic cores with pH / oxidation reduction double-sensitive polymerunits are arranged inside the nanoparticles, and the hydrophobic cores are cross-linked under the action of dithiothreitol, therefore, stable and reversible double-targeting and pH / oxidation reduction double-sensitive core cross-linking nanoparticles are prepared. Medicine-carrying nanoparticles are prepared from adriamycin as a model medicine, and the stability and the pH / reduction double-sensitivity of medicine-carrying cross-linking nanoparticles can be observed through in-vitro medicine release experiments. Results show that the double-targeting and double-sensitive core cross-linking nanoparticles are simple and convenient in preparation method and high in medicine carrying rate, and the nanoparticles have the properties that the nanoparticles are stable in vitro and low in pH valueinside tumor cells and have hydrophilic-hydrophobic transfer with pH / oxidation reduction sensitive polymer units, and medicines can be rapidly released from a cross-linking structure.

The invention provides a sampling and pretreatment device of redox sensitive element containing water. The device comprises a tank, a pull rod, a cover and a piston, a center hole is formed in the center of the top of the tank, a water filter pipe is arranged outside the center of the bottom of the tank, a filter membrane is arranged on the inner side of the center of the bottom of the tank, a shutoff valve is arranged on the water filter pipe, and a pipe opening of the water filter pipe is covered with the filter membrane. A sampling pipe is arranged at the bottom of the side wall of the tank, a water outlet pipe is arranged at the bottom of the sampling pipe, a three-way valve is arranged at the intersection portion of the water outlet pipe and the sampling pipe, the pull rod is sleeved with the tank, the top of the pull rod stretches out of the center hole, and a through shaft core is arranged in the axial center of the pull rod. The piston is connected with the bottom of the pull rod, a through hole is formed in the axial center of the piston, the through hole corresponds to the shaft core, and the cover and the top of the pull rod are connected in a configured mode. According to the sampling and pretreatment device, it can be avoided that redox sensitive elements are in contact with air to generate chemical changes in the water sample collecting process, the authenticity of water samples is guaranteed, the sampling device is directly utilized for suction filtration, cost is reduced, time is saved, efficiency is improved, dividing sampling is achieved, and flexibility and convenience are achieved.

The invention relates to a double-sensitive amphiphilic copolymer containing a Schiff base and a mercapto group on a same side group, as well as a preparation method and application of the double-sensitive amphiphilic copolymer. The copolymer is the amphiphilic copolymer which introduces an acid-sensitive bond, namely the Schiff base and a redox-sensitive group, namely the mercapto group on the same side group on the basis of a polyethylene glycol / polyester amphiphilic copolymer containing a side carboxyl group in a polyester segment. The copolymer can form acid-sensitive and redox-sensitive reversible cross-linked nanoparticles with physical entrapment of a medicament by self-assembly, namely that a disulfide bond is formed by oxidation of the mercapto group for cross-linking, and the fracture of the mercapto group or the Schiff base is produced by reduction of the disulfide bond, thereby disintegrating a cross-linked structure. The fracture of any bond of the Schiff base and the disulfide bond can promote the disintegration of the double-sensitive polymer nanoparticles and the release of the medicament and further improve the bioavailability of the medicament in an acidic micro-environment of tumor cells and high concentration of glutathione (GSH).

The invention discloses a redox-sensitive hyaluronic acid-docetaxel conjugate and a preparation method thereof. The redox-sensitive hyaluronic acid-docetaxel conjugate is prepared by the following steps: mixing a raw material medicine with a connection body, dissolving the mixture into an organic solvent, adding an acid-binding agent, stirring under room temperature, and performing vacuum spinning steaming to remove the organic solvent; after purification is executed, dissolving a product into a proper amount of the solvent, adding an activating agent and a dewatering agent for activation under room temperature to generate anhydride activation ester serving as the raw material medicine; dissolving a carrier into a solution, and adding the activating agent and the dewatering agent for stirring activation; after activation is executed, adding a disulfide bond crosslinking agent, performing dialysis, dry-freezing to obtain a product; dissolving a carrier crosslinking product into the organic solvent; dripping the anhydride activation ester serving as the raw material medicine into a carrier solution, incubating for certain time, performing dialysis, and dry-freezing to obtain the target product. The conjugate prepared by the invention can effectively improve the solubility of docetaxel; the redox-sensitive hyaluronic acid-docetaxel conjugate is higher in targeting property and has the advantages of simple preparation method, low cost and the like.

The invention discloses a cross-linked nano-micelle with redox sensitive performance and a preparation method of the cross-linked nano-micelle. The preparation method comprises the following steps: a reversible addition-fragmentation chain transfer polymerization method is adopted, a macromolecular chain transfer agent is synthesized firstly and polymerized with poly(ethylene glycol) methacrylate to synthesize a block polymer which can be further modified, on the basis of self-assembly, a cross-linking agent having chemical linking capacity and containing a disulfide bond is introduced, and one stably cross-linked nano-micelle with the redox response performance is constructed in a mode of assembly and later cross-linking. The method is easy to control, the reaction conditions are mild, a metal catalyst is avoided, a product is easy to purify, and the obtained nano-micelle not only can package a hydrophobic drug but also can prevent the drug from being released in advance in blood circulation.

The invention discloses a Fe3O4 grafted copolymer heterozygote with multiple stimulus responses, and a preparation method and an application thereof. The heterozygote provided by the invention is composed of magnet-responsive Fe3O4, pH-sensitive polyacrylic acid, and redox-sensitive polymethacrylate ferrocenylformyloxyethyl ester. The preparation method comprises the following preparation process:introducing a Fe3O4 surface chain transfer agent, and grafting polyacrylic acid and polymethacrylate ferrocenylformyloxyethyl ester onto the surface of Fe3O4 through two continuous steps of reversible addition-fragmentation chain transfer polymerization so as to obtain a Fe3O4 grafted acrylic acid-methacrylate ferrocenylformyloxyethyl ester segmented copolymer heterozygote. The heterozygote provided by the invention can be self-assembled into a spherical core-shell micelle in water, shows stimulus responsiveness to magnet, pH and redox, has the characteristic of releasing multiple stimulus responses to an anti-cancer drug namely paclitaxel, and can be used as a carrier of a hydrophobic drug and applied to targeted therapy of cancer.

The invention discloses a preparation method and an application of a redox sensitive induced pH-responsive nano drug carrier. The carrier is obtained through self-assembly of a PEDE (PEG (polyethyleneglycol)-poly-AMA-DNBSE (2-((2,4-dinitro-N-ethyl)phenyl sulfonamide)ethyl methacrylate)) amphiphilic polymer in an aqueous solution. Polymer nanoparticles have clear core-shell structures, carry hydrophobic drugs according to a similarity and intermiscibility principle and have stable properties and a passive targeted transfer function. Hydrophobic chain segments of the polymer nanoparticles contain strong electron withdrawing groups 2,4-dinitro-phenyl sulfonamide structures and can be subjected to a nucleophilic substitution reaction with sulfydryl quickly to produce secondary amine groups, and the secondary amine groups have protonation to cause disturbance to assembly stability of the nano carrier, so that the redox sensitive induced pH-responsive drug release is realized.

Embodiments of the present invention provide for a transgenic plan, methods of making and DNA constructs for use in the transgenic plant which transgenic plant is capable of modulating its photosynthetic antenna complex composition in response to increases or decreases in light intensity by modulation of the ratio of chlorophyll a to chlorophyll b such that there is an increase in the Chl a / b ratio at high light intensity and a decrease in the Chl a / b ratio at low light intensity versus wild-type plants grown in the same conditions.

The invention relates to the technical field of biomedical materials. The method comprises the steps of firstly synthesizing a yellow gelatinous compound thiocinamide from lipoic acid and ethylenediamine under an N,N'-carbonyl diimidazole catalyst; carrying out polymerization by using thiocinamide, ethylene glycol diglycidyl ether and lysine through a nucleophilic addition mechanism to prepare a poly(lysine-co-ethylene glycol diglycidyl ether-co-thiocinamide) terpolymer, and then forming a zwitter-ion nano micelle in a selective solvent through self-assembly. The micelle can be endowed with excellent anti-protein nonspecific adsorption and enhanced cell uptake property through protonation / deprotonation action under different pH conditions; a disulfide bond in lipoyl can form a linear polydisulfide structure under the action of 1,4-dithiothreitol, so that a micelle core is crosslinked and a cross-linked structure is destroyed in the cell, and controlled release of a drug can be achieved. The nano micelle is expected to be a drug carrier for treating cancers.

The invention relates to a preparation method of a nano delivery system between targeted redox-sensitive co-load chemotherapeutic drugs and P-gp resistance reversal agents. The redox-sensitive amphiphilic copolymers PCL7500-ss-PEG7500-ss-PCL7500 is utilized to build redox-sensitive polymer vesicle, intermolecular hydrophobic force is used to load hydrophobic taxol and Tarigquidar, adriamycin is loaded inside the hydrophilic chamber of the polymer vesicle via pH gradient method, folate targeting group is decorated on the surface of the polymer vesicle via covalent bond, thereby the polymer vesicle with synergistic activity with targeting, reduction and response and chemotherapy is built. P-glycoprotein(P-gp) of small molecules inhibitor Tariguidar reduces the drug resistance of drug resistance cells by blocking the efflux function of P-gp to the base drug, the picking up of drugs of drug resistance cells is increased, thereby the multiple drug resistance is reversed. The nano delivery system is capable of loading hydrophobic drugs and hydrophilic drugs, tumor targeting and having reducing and response to the tumor micro environment and realizing killing tumors by reserving the multiple drug resistance.

The invention discloses an enzyme-sensitive and redox-sensitive dual response type copolymer as well as a preparation method and application thereof and belongs to the fields of high polymer chemistryand pharmaceutical preparations. The preparation method in the invention comprises the following steps: binding polyacrylic acid and vitamin E succinate by utilizing valine-citrulline (Val-Cit, VC) fragments capable of being specifically hydrolyzed by cathepsin B and disulfide bonds capable of being reduced by glutathione, thereby obtaining the dual sensitive type copolymer polyacrylic acid-VC-SS-vitamin E succinate (PAA-VC-SS-VES). The copolymer is excellent in biocompatibility and high in sensitivity, can be self-assembled to form nano-micelles in water, can be coated with hydrophobic drugs, is mainly applied to treating tumor related diseases and has an effect of intelligently releasing the drugs.

The invention provides a dual-sensitive targeted nanoparticle preparation for loading chemotherapeutic drugs and a preparation method. The nanoparticle preparation comprises an amphiphilic polymer. The amphiphilic polymer comprises gelatin and at least one chemotherapeutic drug molecule. Each chemotherapeutic drug molecule is chemically bonded to the gelatin via a first chemical group, and the backbone of each first chemical group comprises a disulfide bond. The disulfide bonds with redox sensitivity can be broken to release drugs when the concentration of glutathione around tumor tissues is high. The gelatin, as a substrate of an MMP-2 enzyme, can be degraded into small particles around the tumor tissues by the overexpressed MMP-2 enzyme, so that the permeability in the tumor tissues is improved, and the dual-sensitive drugs are released.

The invention discloses a triple stimulation responsive core cross-linked polymeric micelle as well as a preparation method and application thereof. The structural formula of the micelle is as shown in the description, wherein x is an integer of 30 to 70, y is an integer of 20 to 50 and the value of x1 is 20% to 50% of that of the x. According to the preparation method, an amphipathic diblock polymer is obtained through atom transfer radical polymerization (ATRP), and is modified by utilizing an azidation reaction; an azidation product can self-assemble to form a core-shell structure in water;and the light, oxidation and reduction triple stimulation responsive core cross-linked polymeric micelle is obtained through click chemistry under the action of a cross-linking agent with redox sensitiveness, anhydrous cupric sulfate and sodium ascorbate. The triple stimulation responsive core cross-linked polymeric micelle provided by the invention has favorable in-vitro stability, can be used for realizing a high-efficiency controlled release behavior of a hydrophobic drug under the double stimulation of light and oxidation or reduction, and has favorable application prospects in the aspects of the delivery and the controlled release of a hydrophobic anti-cancer drug.

The invention belongs to the technical field of pharmacy, and particularly relates to a preparation method and application of polyethylene glycol-deoxycholic acid and derivatives of polyethylene glycol-deoxycholic acid. A parent nucleus of the polyethylene glycol-deoxycholic acid derivative is polyethylene glycol-deoxycholic acid, a joint key is an oxidation and reduction sensitive key, a pH sensitive key or an enzymatic hydrolytic key, and a ligand is a hydrophobic amino acid compound, a bio-compatible polymer or an insoluble anticancer drug. The preparation method of the polyethylene glycol-deoxycholic acid comprises the following steps: dissolving deoxycholic acid and 4-dimethylaminopyridine in anhydrous dichloromethane to obtain a deoxycholic acid solution, then dissolving polyethyleneglycol in dichloromethane to obtain a polyethylene glycol solution, then gradually dropwise adding the deoxycholic acid solution and the polyethylene glycol solution into a reaction bottle, adding acatalyst, and obtaining polyethylene glycol-deoxycholic acid by virtue of reaction. The polyethylene glycol-deoxycholic acid and derivatives of polyethylene glycol-deoxycholic acid prepared by the invention have good anti-multidrug resistance and a good application prospect.

The invention discloses a polyacrylic acid-S-S-drug copolymer and a preparation method thereof, and belongs to the fields of polymer chemistry and medicinal preparations. A drug derivative is preparedfrom a drug and cystamine dihydrochloride; and polyacrylic acid and the drug derivative are condensed to form the polyacrylic acid-S-S-drug copolymer. The polyacrylic acid-S-S-drug copolymer can spontaneously form an amphiphilic polymer micelle in an aqueous solution, the linkage bond is a disulfide bond, and the linkage bond can responsively rupture at the lesion to release the drug; and additionally, the polyacrylic acid can well improve the water solubility of the drug, and can be used to prepare a redox-sensitive polymer prodrug for improving the solubility of the poorly soluble drug. Theinvention also discloses a preparation method of the PAA-S-S-GA copolymer, and a use of the PAA-S-S-GA copolymer as an anticancer drug carrier.

The invention discloses an oxidoreduction sensitive sericin derivative with the anti-tumor activity and preparation and application thereof, and belongs to the technical field of biological medicine materials. The derivative is composed of hydrophilic sericin and hydrophobic vitamin E succinate which are connected through a disulfide bond; the mass ratio of the sericin to the vitamin E succinate is 20:1-100:1, and the content of the disulfide bond in every 100 amino acid units accounts for 10-25% on average. According to the oxidoreduction sensitive sericin derivative with the anti-tumor activity, under the environment of a water solution, self-assembling can be performed to form a nano-micelle with an internal hydrophobic cavity, a hydrophobic anti-cancer drug can be loaded, bonds can bebroken under the high reduction type glutathione content, and the drug can be rapidly released under the tumor microenvironment. The micelle system can effectively enhance the cancer cell killing effect of drugs, and the good research value and application prospect are achieved in the cancer treatment field.