78 results about "Redox sensitive" patented technology

This invention relates to an irreversible indicator for detecting oxidising agents, or in particular an oxygen indicator, comprising at least one redox-sensitive dyestuff, at least one semiconductor material and at least one electron donor. This indicator is activated by exposure to light of about 200-400 nm. The invention also relates to UV light detector.

A medium for isolating or releasing an electrostatically charged component from or into an aqueous composition. The medium has a polyelectrolyte film on at least one surface of an article wherein the polyelectrolyte film is characterized by an interpenetrating network of a predominantly positively charged polymer and a predominantly negatively charged polymer. The predominantly positively charged polymer, the predominantly negatively charged polymer or both contain (i) a pH sensitive imidazole repeat unit having a pKa between 3 and 9, or (ii) a redox sensitive repeat unit selected from the group consisting of quaternized bipyridine repeat units, coordinated metal repeat units, pyrrole repeat units, aniline repeat units, thiophene repeat units and combinations thereof having a redox potential between +1.2 volts and −1.2 volts versus a standard hydrogen electrode.

The invention relates to a dual sensitive amphiphilic triblock copolymer containing disulfide bond and acylhydrazone bond and a preparation method and an application of the dual sensitive amphiphilic triblock copolymer. A BAB type amphiphilic triblock copolymer consists of a hydrophobic polyester block A and a hydrophilia polyethylene glycol block B, wherein the block A is polyether containing active terminal amino group and disulfide bond; the block B is benzaldehyde ester of an ethylene glycol monomethyl ether or single terminated polyoxyethylene polypropylene oxide copolymer; the block A and the block B are connected through acid sensitive acylhydrazone bond generated from reaction of active terminal amino group and aldehyde group. The dual sensitive amphiphilic triblock copolymer is good in biocompatibility and biodegradability, can be formed into nanoparticles with oxidation reduction sensitivity and acid sensitivity in a water medium in a self-assembling manner, can wrap hydrophobic medicines to form nano medicine preparations, and can promote rapid release of medicines in tumor cells due to fracture of disulfide bond and acylhydrazone bond which are sensitive to oxidation reduction in a high concentration glutathione and acid micro environment in the tumor cells.

The invention relates to a preparation method and application of hyaluronic acid modified by vitamin E succinate and is capable of effectively solving the problems of preparation of hyaluronic acid modified by vitamin E succinate and the application in antitumor drugs. The preparation method comprises the following steps: with alkylidene diamine or dithio alkylidene diamine as a linking arm, hyaluronic acid is covalently linked to vitamin E succinate according to the weight ratio of 1:(1-100) so as to form a nano-micelle in a water medium, wherein hyaluronic acid is low-molecular-weight hyaluronic acid with the molecular weight no less than 600 daltons and no larger than 400kd; the linking arm is the alkylidene diamine or the dithio alkylidene diamine with the amount of carbon atoms being 2-12, and a disulfide bond in the dithio alkylidene diamine has the oxidation-reduction sensitivity in vivo and is capable of releasing the antitumor drugs in a targeting mode in tumor cells. With HA as a skeleton, hyaluronic acid modified by vitamin E succinate is high in drug loading capacity and easy in releasing of the drugs, is effectively used for preparing the antitumor drugs, is capable of solving the problem of medication for treating tumors and is an innovation of the drugs for treating the tumors.

The disclosure provides proteins that can be used to determine the redox status of an environment (such as the environment within a cell or subcellular compartment). These proteins are green fluorescent protein (GFP) variants (also referred to as redox sensitive GFP (rosGFP) mutants), which have been engineering to have two cysteine amino acids near the chromophore and within disulfide bonding distance of each other. Also provided are nucleic acid molecules that encode rosGFPs, vectors containing such encoding molecules, and cells transformed therewith. The disclosure further provides methods of using the rosGFPs (and encoding molecules) to analyze the redox status of an environment, such as a cell, or a subcellular compartment within a cell. In certain embodiments, both redox status and pH are analyzed concurrently.

The invention belongs to the technical field of medicines, and relates to a redox sensitive albumin binding prodrug with a series of tumor tissue specific responses. The albumin binding prodrug is ananti-tumor drug-maleimide molecule prodrug particularly including three docetaxel-maleimide small molecule prodrugs; by utilizing a maleimide ring in a prodrug structure as a target head of plasma albumin 34-bit free sulfydryl in a binding body, so that after a drug enters into a body through intravenous injection, the drug is quickly and specifically bound with albumin to form an albumin-drug composition; therefore, the drug stability is enhanced, the circulation time of the drug in the boy is obviously prolonged, and accumulation of the drug on tumor tissues is realized by utilizing an EPR (Enhanced Permeability and Retention) effect. In addition, in the prodrug structure, by using a redox sensitive key as a binding key, quick release of the drug in tumor cells is promoted, a toxic effect of the drug on normal cells also can be alleviated, the prodrug has high selectivity, the aims of increasing an effect and reducing the toxicity of docetaxel are fulfilled, and a greater market application prospect is achieved.

The invention relates to a pH-redox double sensitive amphiphilic polymer of which the structural formula is disclosed in the specification, wherein X=15-35, Y=1-2, PEG is a polyethyleneglycol monomethyl ether chain segment, and the molecular weight is 9500-25000. The preparation method comprises the following steps: mixing raw materials, reacting in an oil bath in a nitrogen atmosphere, eluting, dissolving in dichloromethane, adding petroleum ether to precipitate under ice bath conditions, standing, centrifugating to remove solvent, dissolving in dichloromethane, flushing, carrying out centrifugal drying, and drying in an oven. By introducing the pH-sensitive monomer and redox-sensitive crosslinking agent into the polymer structure, the pH-redox double sensitive amphiphilic polymer has favorable pH sensitivity and redox sensitivity, can be used as a drug carrier material, has environment response capacity, can make a response according to the environmental change when arriving at the target spot position, and effectively implements controlled release of drugs.

The invention discloses a multifunctional supramolecular gene delivery system based on polyethyleneimine-cyclodextrin and a preparation method thereof. A polyethyleneimine-cyclodextrin polycationic compound serves as a framework, adamantane-disulfide bond-polyethylene glycol having oxidation reduction reactivity and adamantane-polyethylene glycol-targeting peptide having targeting performance serve as modified groups, polyethyleneimine-cyclodextrin is combined with adamantane-disulfide bond-polyethylene glycol and adamantane-polyethylene glycol-targeting peptide through a self-assembling principle, and a subjective body and an objective body act to form a supramolecular gene carrier. The delivery system is multifunctional, has oxidation reduction sensitivity, specificity for targeting liver cancer and excellent gene transfection efficiency, and can carry functional genes to efficiently treat liver malignant tumor.

The invention provides a sampling and pretreatment device of redox sensitive element containing water. The device comprises a tank, a pull rod, a cover and a piston, a center hole is formed in the center of the top of the tank, a water filter pipe is arranged outside the center of the bottom of the tank, a filter membrane is arranged on the inner side of the center of the bottom of the tank, a shutoff valve is arranged on the water filter pipe, and a pipe opening of the water filter pipe is covered with the filter membrane. A sampling pipe is arranged at the bottom of the side wall of the tank, a water outlet pipe is arranged at the bottom of the sampling pipe, a three-way valve is arranged at the intersection portion of the water outlet pipe and the sampling pipe, the pull rod is sleeved with the tank, the top of the pull rod stretches out of the center hole, and a through shaft core is arranged in the axial center of the pull rod. The piston is connected with the bottom of the pull rod, a through hole is formed in the axial center of the piston, the through hole corresponds to the shaft core, and the cover and the top of the pull rod are connected in a configured mode. According to the sampling and pretreatment device, it can be avoided that redox sensitive elements are in contact with air to generate chemical changes in the water sample collecting process, the authenticity of water samples is guaranteed, the sampling device is directly utilized for suction filtration, cost is reduced, time is saved, efficiency is improved, dividing sampling is achieved, and flexibility and convenience are achieved.

The invention discloses a redox-sensitive hyaluronic acid-docetaxel conjugate and a preparation method thereof. The redox-sensitive hyaluronic acid-docetaxel conjugate is prepared by the following steps: mixing a raw material medicine with a connection body, dissolving the mixture into an organic solvent, adding an acid-binding agent, stirring under room temperature, and performing vacuum spinning steaming to remove the organic solvent; after purification is executed, dissolving a product into a proper amount of the solvent, adding an activating agent and a dewatering agent for activation under room temperature to generate anhydride activation ester serving as the raw material medicine; dissolving a carrier into a solution, and adding the activating agent and the dewatering agent for stirring activation; after activation is executed, adding a disulfide bond crosslinking agent, performing dialysis, dry-freezing to obtain a product; dissolving a carrier crosslinking product into the organic solvent; dripping the anhydride activation ester serving as the raw material medicine into a carrier solution, incubating for certain time, performing dialysis, and dry-freezing to obtain the target product. The conjugate prepared by the invention can effectively improve the solubility of docetaxel; the redox-sensitive hyaluronic acid-docetaxel conjugate is higher in targeting property and has the advantages of simple preparation method, low cost and the like.

The invention discloses a cross-linked nano-micelle with redox sensitive performance and a preparation method of the cross-linked nano-micelle. The preparation method comprises the following steps: a reversible addition-fragmentation chain transfer polymerization method is adopted, a macromolecular chain transfer agent is synthesized firstly and polymerized with poly(ethylene glycol) methacrylate to synthesize a block polymer which can be further modified, on the basis of self-assembly, a cross-linking agent having chemical linking capacity and containing a disulfide bond is introduced, and one stably cross-linked nano-micelle with the redox response performance is constructed in a mode of assembly and later cross-linking. The method is easy to control, the reaction conditions are mild, a metal catalyst is avoided, a product is easy to purify, and the obtained nano-micelle not only can package a hydrophobic drug but also can prevent the drug from being released in advance in blood circulation.

The invention discloses a Fe3O4 grafted copolymer heterozygote with multiple stimulus responses, and a preparation method and an application thereof. The heterozygote provided by the invention is composed of magnet-responsive Fe3O4, pH-sensitive polyacrylic acid, and redox-sensitive polymethacrylate ferrocenylformyloxyethyl ester. The preparation method comprises the following preparation process:introducing a Fe3O4 surface chain transfer agent, and grafting polyacrylic acid and polymethacrylate ferrocenylformyloxyethyl ester onto the surface of Fe3O4 through two continuous steps of reversible addition-fragmentation chain transfer polymerization so as to obtain a Fe3O4 grafted acrylic acid-methacrylate ferrocenylformyloxyethyl ester segmented copolymer heterozygote. The heterozygote provided by the invention can be self-assembled into a spherical core-shell micelle in water, shows stimulus responsiveness to magnet, pH and redox, has the characteristic of releasing multiple stimulus responses to an anti-cancer drug namely paclitaxel, and can be used as a carrier of a hydrophobic drug and applied to targeted therapy of cancer.

Embodiments of the present invention provide for a transgenic plan, methods of making and DNA constructs for use in the transgenic plant which transgenic plant is capable of modulating its photosynthetic antenna complex composition in response to increases or decreases in light intensity by modulation of the ratio of chlorophyll a to chlorophyll b such that there is an increase in the Chl a/b ratio at high light intensity and a decrease in the Chl a/b ratio at low light intensity versus wild-type plants grown in the same conditions.

The invention relates to a preparation method of a nano delivery system between targeted redox-sensitive co-load chemotherapeutic drugs and P-gp resistance reversal agents. The redox-sensitive amphiphilic copolymers PCL7500-ss-PEG7500-ss-PCL7500 is utilized to build redox-sensitive polymer vesicle, intermolecular hydrophobic force is used to load hydrophobic taxol and Tarigquidar, adriamycin is loaded inside the hydrophilic chamber of the polymer vesicle via pH gradient method, folate targeting group is decorated on the surface of the polymer vesicle via covalent bond, thereby the polymer vesicle with synergistic activity with targeting, reduction and response and chemotherapy is built. P-glycoprotein(P-gp) of small molecules inhibitor Tariguidar reduces the drug resistance of drug resistance cells by blocking the efflux function of P-gp to the base drug, the picking up of drugs of drug resistance cells is increased, thereby the multiple drug resistance is reversed. The nano delivery system is capable of loading hydrophobic drugs and hydrophilic drugs, tumor targeting and having reducing and response to the tumor micro environment and realizing killing tumors by reserving the multiple drug resistance.

The invention discloses an enzyme-sensitive and redox-sensitive dual response type copolymer as well as a preparation method and application thereof and belongs to the fields of high polymer chemistryand pharmaceutical preparations. The preparation method in the invention comprises the following steps: binding polyacrylic acid and vitamin E succinate by utilizing valine-citrulline (Val-Cit, VC) fragments capable of being specifically hydrolyzed by cathepsin B and disulfide bonds capable of being reduced by glutathione, thereby obtaining the dual sensitive type copolymer polyacrylic acid-VC-SS-vitamin E succinate (PAA-VC-SS-VES). The copolymer is excellent in biocompatibility and high in sensitivity, can be self-assembled to form nano-micelles in water, can be coated with hydrophobic drugs, is mainly applied to treating tumor related diseases and has an effect of intelligently releasing the drugs.

The invention discloses a triple stimulation responsive core cross-linked polymeric micelle as well as a preparation method and application thereof. The structural formula of the micelle is as shown in the description, wherein x is an integer of 30 to 70, y is an integer of 20 to 50 and the value of x1 is 20% to 50% of that of the x. According to the preparation method, an amphipathic diblock polymer is obtained through atom transfer radical polymerization (ATRP), and is modified by utilizing an azidation reaction; an azidation product can self-assemble to form a core-shell structure in water;and the light, oxidation and reduction triple stimulation responsive core cross-linked polymeric micelle is obtained through click chemistry under the action of a cross-linking agent with redox sensitiveness, anhydrous cupric sulfate and sodium ascorbate. The triple stimulation responsive core cross-linked polymeric micelle provided by the invention has favorable in-vitro stability, can be used for realizing a high-efficiency controlled release behavior of a hydrophobic drug under the double stimulation of light and oxidation or reduction, and has favorable application prospects in the aspects of the delivery and the controlled release of a hydrophobic anti-cancer drug.

The invention belongs to the technical field of pharmacy, and particularly relates to a preparation method and application of polyethylene glycol-deoxycholic acid and derivatives of polyethylene glycol-deoxycholic acid. A parent nucleus of the polyethylene glycol-deoxycholic acid derivative is polyethylene glycol-deoxycholic acid, a joint key is an oxidation and reduction sensitive key, a pH sensitive key or an enzymatic hydrolytic key, and a ligand is a hydrophobic amino acid compound, a bio-compatible polymer or an insoluble anticancer drug. The preparation method of the polyethylene glycol-deoxycholic acid comprises the following steps: dissolving deoxycholic acid and 4-dimethylaminopyridine in anhydrous dichloromethane to obtain a deoxycholic acid solution, then dissolving polyethyleneglycol in dichloromethane to obtain a polyethylene glycol solution, then gradually dropwise adding the deoxycholic acid solution and the polyethylene glycol solution into a reaction bottle, adding acatalyst, and obtaining polyethylene glycol-deoxycholic acid by virtue of reaction. The polyethylene glycol-deoxycholic acid and derivatives of polyethylene glycol-deoxycholic acid prepared by the invention have good anti-multidrug resistance and a good application prospect.

The invention discloses an oxidoreduction sensitive sericin derivative with the anti-tumor activity and preparation and application thereof, and belongs to the technical field of biological medicine materials. The derivative is composed of hydrophilic sericin and hydrophobic vitamin E succinate which are connected through a disulfide bond; the mass ratio of the sericin to the vitamin E succinate is 20:1-100:1, and the content of the disulfide bond in every 100 amino acid units accounts for 10-25% on average. According to the oxidoreduction sensitive sericin derivative with the anti-tumor activity, under the environment of a water solution, self-assembling can be performed to form a nano-micelle with an internal hydrophobic cavity, a hydrophobic anti-cancer drug can be loaded, bonds can bebroken under the high reduction type glutathione content, and the drug can be rapidly released under the tumor microenvironment. The micelle system can effectively enhance the cancer cell killing effect of drugs, and the good research value and application prospect are achieved in the cancer treatment field.

The invention belongs to the technical field of medicines, and relates to a chemotherapeutic drug-hypoxic activation prodrug integrated prodrug. A chemotherapeutic drug and a hypoxic activation prodrug are connected through redox sensitive bonds or non-sensitive bonds to realize efficient co-loading and synchronous delivery of the chemotherapeutic drug and the hypoxic activation prodrug. Accordingto the invention, camptothecin is used as the chemotherapeutic drug, PR104A is used as the hypoxic activation prodrug, disulfide bonds or non-sensitive carbonate bonds are used for connection to synthesize the integrated prodrug, and a self-assembled nano drug delivery system is prepared. The preparation process is simple, and the drug loading capacity of nanoparticles is high; the particle sizeis small and uniform, so that the nanoparticles can be enriched at a tumor part through an EPR effect; under the action of high-level glutathione in tumor, disulfide bond fission is triggered to realize quick release of the two kinds of drugs; and the hypoxic activation prodrug can effectively overcome the limitation that the chemotherapeutic drug permeates into the tumor and kill the tumor through an ortho effect, and effective tumor deep permeation and synergistic anti-tumor effects are achieved.