13 results about "Redox responsive" patented technology

The invention discloses a redox sensitive cationic polymer gene vector as well as preparation and application thereof. According to the invention, hydrophobic small molecules with oxidation / reduction responsive S-S bonds are adopted to carry out side chain modification on polyglycidyl amine, and the cationic polymer gene delivery carrier material with specific tumor microenvironment response capability is prepared. The material has good biocompatibility, and can effectively load siRNA molecules and form siRNA compound nanoparticles with good stability and excellent gene transfection capacity. The compound nanoparticles can be effectively absorbed by cells, and can effectively respond to an oxidation / reduction tumor microenvironment to realize targeted release of siRNA. The oxidation / reduction response cationic polymer gene vector provided by the invention has a relatively great clinical application prospect.

The invention discloses a pH-responsive HG type pectin hydrogel based on Schiff base reaction and a preparation method and application thereof, the pH-responsive HG type pectin hydrogel has a structural formula as shown in the following formula I. According to the pH-responsive HG type pectin hydrogel, functional characteristics of HG type pectin, biological activity of oleanolic acid, 3, 4, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3-trimethyl-1, 3-trimethyl-1, 3-trimethyl-1, 3- The intelligent hydrogel system with ROS / pH dual response characteristics is successfully constructed through the redox responsiveness of oleanolic acid, 3, 3 '-dithiodipropionic acid and the pH responsiveness of Schiff base reaction, and the system can effectively protect the activity of oleanolic acid and can be used for preparing drugs for improving alcoholic liver injury.

The invention discloses a redox response type yeast microcapsule drug loading and a preparation method and application thereof, and belongs to the technical field of yeast microcapsule drug loading, and the preparation method comprises the following steps: dissolving a yeast microcapsule in an abamectin solution, carrying out pretreatment to obtain a precipitate, and carrying out freeze drying treatment on the precipitate to obtain a drug-loaded yeast microcapsule; putting the drug-loaded yeast microcapsule into a polyethyleneimine solution, and stirring to obtain a drug-loaded microcapsule solution adsorbed with polyethyleneimine; a photoinitiator and allicin are added into a drug-loaded microcapsule solution adsorbing polyethyleneimine for a polymerization reaction, the drug-loaded redox response type yeast microcapsule is obtained, and the technical problem that in the prior art, due to the fact that a pesticide preparation prepared through a preparation method cannot respond to environmental signals, the targeted transmission efficiency is low is solved.

This application provides a polymer for encapsulating protein drugs, redox-responsive nanoparticles made from the polymer, and their applications. The structure of the polymer is shown in the following general formula I. Protein drug-encapsulated nanoparticles can be prepared by encapsulating protein drugs with the polymer. These nanoparticles can efficiently load protein drugs, disassemble in the redox microenvironment of tumors, and release the drug, thus providing a new technical means for the targeted delivery of protein drugs.

The application discloses a kind of oxidation-reduction response films and preparation method and application thereof, it is related to membrane separation technical field.The method includes that Fc is dissolved in organic solvent, GO is added, and mixed solution A is obtained;GO and CD are added in water, and mixed solution B is obtained;After mixed solution A and mixed solution B are ultrasonic dispersion treatment, respectively under heating condition reaction, then by dialysis purification, freeze-drying treatment, respectively obtain GO-Fc and GO-CD;GO-Fc and GO-CD are dissolved in water, and they are mixed uniformly by ultrasonic dispersion, and the GO-Fc / GO-CD film, i.e. oxidation-reduction response film is prepared by vacuum assisted self-assembly method on substrate membrane and is extracted and is prepared.GO sheet layer nanometer channel size can be adjusted by changing the oxidation-reduction state of GO-Fc / GO-CD film, to realize the reversible regulation of membrane water permeability and molecular separation performance.In addition, the prepared GO-Fc / GO-CD film has excellent cycle response performance and stability, and shows wide application prospect in intelligent response gate and pollution removal separation.

The application discloses a redox response carrier protein and application thereof in preparation of vaccines, wherein at least one hapten structure is chemically coupled and modified on the carrier protein through a connecting arm containing a disulfide bond structure, and the hapten structure is selected from one of dinitrobenzene, galactose-alpha-(1,3)-galactose, rhamnose and phosphorylcholine. The redox response disulfide bond connecting arm can modify the hapten to the carrier protein, and can be recognized and combined by the naturally existing antibody in the body to form a complex, promote phagocytosis, processing and presentation of antigen presenting cells, thereby effectively improving the immunogenicity of the vaccine molecule and improving the antigen related immune response. After the redox disulfide bond connecting arm enters the lysosome, the disulfide bond is broken through response to the reducing property in the cell, lysosome escape occurs, cross-layer delivery of the antigen is realized, and the immunogenicity of the antigen is greatly improved.

Disclosed herein are compositions and methods of making and use thereof. For example, disclosed herein are pharmaceutical compositions comprising: a plurality of redox-responsive disulfide nanoparticles; and a tyrosine kinase inhibitor encapsulated within each of the redox-responsive disulfide nanoparticles. Also disclosed herein are methods of treating or preventing an ocular injury, disease, or disorder in a subject in need thereof, the methods comprising administering a therapeutically effective amount of a plurality of redox-responsive disulfide nanoparticles and / or any of the pharmaceutical compositions disclosed herein to the subject.

The invention provides an HE4siRNA (Hepatitis E 4 Small Interference Ribonucleic Acid) loaded nano particle with redox response. The nano particle is prepared from a tumor targeting carrier material with redox response and HE4siRNA. The tumor targeting carrier material with redox response is poly (ethyleneimine)-diselenide bond-poly (ethylene glycol) (PEI-SeSe-PEG) modified by c (RGDfK) peptide. According to the nanoparticle, a carrier material and siRNA are mixed according to the N / P ratio of 10: 1 and are self-assembled into the nanoparticle, the particle size is about 116 nm, in-vitro release has oxidation-reduction responsiveness, release of siRNA is achieved in a tumor microenvironment of high-concentration glutathione (GSH), and the nanoparticle is beneficial to play a role in a tumor site. The HE4siRNA loaded nanoparticles with redox response prepared by the invention can realize lysosome escape, have tumor targeting property, can inhibit proliferation, migration and invasion of cis-platinum drug-resistant ovarian cancer cells, and have good tumor targeting property, antitumor activity and biocompatibility in animal bodies. The nanoparticle is simple in preparation method and remarkable in anti-cancer effect, and has good application value and market prospect.

This invention relates to the synergistic anticancer application of redox-responsive condensates (FFSSFF) for delivering jujube extract and 3-methyladenine. Specifically, this invention relates to the application of redox-responsive condensates in the preparation of targeted drug delivery products, wherein the targeted drug delivery products release drugs in response to glutathione. The active ingredients in the targeted drug delivery products are jujube extract and 3-methyladenine. This invention utilizes an FFSSFF carrier designed based on the LLPS principle, integrating redox-sensitive disulfide bond linkage modules to achieve spatiotemporally controlled drug delivery within a tumor tissue-specific microenvironment with high glutathione concentrations.

This invention provides oleanolic acid-modified HG-type pectin nanomicelles, their preparation method, and applications. The preparation method of the HG-type pectin nanomicelles includes: esterifying 3,3'-dithiodipropionic acid and oleanolic acid to obtain SS-OA; esterifying SS-OA and HG-type pectin to obtain oleanolic acid-modified HG-type pectin; dissolving the oleanolic acid-modified HG-type pectin and dispersing it ultrasonically to obtain oleanolic acid-modified HG-type pectin nanomicelles. The oleanolic acid-modified HG-type pectin nanomicelles of this invention combine the functional activities of HG-type pectin and oleanolic acid, as well as the redox responsiveness of 3,3'-dithiodipropionic acid, constructing nanomicelles that respond to ROS generated during alcohol metabolism. They exhibit amphiphilicity and good biocompatibility, and can be used to prepare drugs to alleviate chronic alcoholic liver injury.

The invention discloses a PVA (Polyvinyl Alcohol) material based on redox-pH (Potential of Hydrogen) dual response as well as a preparation method and application thereof, and relates to the technical field of PVA materials. Through selective oxidation of periodate on PVA, an aldehyde group functional group is introduced to a PVA main chain, and subsequently modifiable active sites are constructed. A pharmaceutically acceptable salt of cystamine is used as a difunctional cross-linking agent, and is efficiently subjected to a Schiff base reaction with an aldehyde group in PVA to form a dynamic imine bond network, so that the PVA material is endowed with a pH response characteristic; meanwhile, a central disulfide bond in a cystamine molecule is introduced into a three-dimensional cross-linked network in situ, so that the material is endowed with excellent redox responsiveness on the premise of keeping the completeness of the cystamine molecule. The process is mild in condition, low in potential toxicity and high in reaction speed, the whole crosslinking process can be completed within several hours, the preparation period is greatly shortened, and the production efficiency is improved.

The invention relates to the technical field of novel organic silicon materials and fine chemical engineering, in particular to redox response type cross-linked silicon paste containing fullerene and ferrocene groups as well as a preparation method and application of the redox response type cross-linked silicon paste. The preparation method of the cross-linked silicon paste comprises the following steps: adding a vinyl modified filler, a fullerene cross-linking agent and a resin cross-linking agent into carrier oil under a stirring condition, mixing, adding a catalyst, and carrying out a hydrosilylation reaction to form primary cross-linked silicon paste particles with an interpenetrating network structure; and adding a functional end-capping reagent and a catalyst, continuously reacting, and adding carrier oil after the reaction is finished. The cross-linking density is controllable, the mechanical strength is high, the surface hydrophilic and hydrophobic intelligent switching function is achieved, and intelligent conversion of hydrophilic easy emulsification during processing and hydrophobic strong foam inhibition during use is achieved. The organic silicon defoaming agent is applied to a defoaming agent, hydrophilic in an oxidation state assists emulsification, hydrophobic in a reduction state assists foam inhibition, and the contradiction that the emulsification stability and the foam inhibition durability of a traditional organic silicon defoaming agent are difficult to consider at the same time is solved.

The invention discloses preparation of a tea oil triterpene alcohol redox response molecularly imprinted polymer and application of the molecularly imprinted polymer in skin care. The preparation method comprises the following steps: dissolving triterpene alcohol and acrylamide in absolute ethyl alcohol, and then adding N, N '-bis (acryloyl) cystamine, a cross-linking agent and an initiator for polymerization reaction to prepare the triterpene alcohol redox response molecularly imprinted polymer. The molecularly imprinted polymer is suitable for directional enrichment of triterpene alcohol in tea oil and targeted release of triterpene alcohol at an inflammation part, has the effects of rapidly eliminating inflammation and recovering healthy skin, and can be applied to the field of cosmetics.