Method for preparing stable albumin nanoparticle

A technology of albumin nano and albumin, which is applied in the field of preparation of albumin nanoparticles, and can solve the problems of difficult delivery of pharmacologically active substances in vivo

Inactive Publication Date: 2013-03-27
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for preparing stable albumin nanoparticles, which are stabilized by cross-linking intermolecular disulfide bonds, so as to solve the problem that certain pharmacologically active substances are difficult to deliver in vivo

Method used

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  • Method for preparing stable albumin nanoparticle
  • Method for preparing stable albumin nanoparticle
  • Method for preparing stable albumin nanoparticle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1, preparation of disulfide bond-stabilized human serum albumin nanoparticles.

[0038] Using human serum albumin and reduced glutathione as raw materials, disulfide bond-stabilized human serum albumin nanoparticles were prepared.

[0039] The specific preparation method is as follows:

[0040]Dissolve 20 mg of human serum albumin in 1 mL of aqueous solution, add 20 mM reduced glutathione, react at room temperature for 1 hour, then add 4 mL of absolute ethanol to react for 30 minutes, and then dialyze the prepared suspension bag, dialyzed in deionized water at 4°C for 24 hours to obtain disulfide bond-stabilized human serum albumin nanoparticles, and the average particle size of albumin nanoparticles is 100-300nm (ZetaPALS, Zeta Potential Analyzer ).

[0041] The stability of the prepared nanoparticles in different solvent environments can be obtained from figure 1 seen in. The stepwise dissolution process of the as-prepared nanoparticles under reducing envi...

Embodiment 2

[0042] Example 2, preparation of disulfide bond-stabilized human serum albumin nanoparticles.

[0043] Using human serum albumin and reduced glutathione as raw materials, disulfide bond-stabilized human serum albumin nanoparticles were prepared.

[0044] The specific preparation method is as follows:

[0045] Dissolve 200 mg of human serum albumin in 1 mL of aqueous solution, add 500 mM reduced glutathione, react at 50°C for 10 minutes, then add 1 mL of absolute ethanol for 5 minutes, and then dialyze the obtained suspension The bag is dialyzed in deionized water at 10° C. for 24 hours to obtain disulfide bond-stabilized human serum albumin nanoparticles, and the average particle size of the albumin nanoparticles is 100-400 nm.

Embodiment 3

[0046] Example 3, preparing a disulfide bond-stabilized curcumin-human serum albumin nanoparticle drug-loading system.

[0047] Human serum albumin, reduced glutathione and curcumin were used as raw materials to prepare a drug-carrying nanoparticle system of curcumin coated with human serum albumin stabilized by disulfide bonds.

[0048] The specific preparation method is as follows:

[0049] Dissolve 40 mg of human serum albumin in 1 mL of aqueous solution, add 50 mM reduced glutathione, react at room temperature for 1 hour, then add 4 mL of 3 mg / mL curcumin ethanol solution and react for 30 minutes, then prepare the obtained The suspension was placed in a dialysis bag, and dialyzed in deionized water at 4° C. for 24 hours to obtain disulfide bond-stabilized curcumin-human serum albumin-coated nanoparticles. Moreover, the average particle size of nanoparticles is 50-400nm (ZetaPALS, Zeta Potential Analyzer), and the drug-loading capacity of curcumin is determined by reversed...

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Abstract

The invention relates to a method for preparing a stable albumin nanoparticle, and belongs to the technical field of preparation of biomedical materials. The method comprises the following steps: pretreating albumin by using glutathione and cysteine without biotoxicity; opening an intramolecular disulfide bond; precipitating the albumin by using anti-solvents such as alcohol and the like; and carrying out exchange reaction on a sulfydryl-disulfide bond to obtain the albumin nanoparticle containing the intramolecular disulfide bond. The prepared albumin nanoparticle can be used for the delivery of pharmacological active substances and / or diagnostic agents in an organism. The albumin nanoparticle provided by the invention has the advantages that the albumin nanoparticle has good stability under a dilution condition and gives an oxidation reduction response in a reduced environment. Based on the characteristics, the albumin nanoparticle can stably exist in a blood circulation system of the organism, and can carry out a degradation reaction in a cell under the action of reduced glutathione so as to release a wrapped medicine.

Description

technical field [0001] The invention relates to a method for preparing albumin nanoparticles, belonging to the technical field of preparation of biomedical materials. Background technique: [0002] Albumin is a biological endogenous protein with many characteristics such as biodegradability, non-toxicity, and non-antigenicity. It is considered to be an ideal drug carrier. research direction. Albumin drug-loaded systems are mainly divided into two categories, one is chemically coupled albumin drug-loaded systems, and the other is physically bound albumin drug-loaded systems. Chemically conjugated albumin can improve the pharmacokinetic properties of the drug, but new chemical molecules are formed after conjugation. The albumin drug delivery system in the form of physical combination is a more ideal drug loading mode. Encapsulating drug molecules in albumin nanoparticles can significantly improve the stability of water-insoluble drugs in aqueous solution (that is, in the blo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42A61K9/14A61K9/19A61K45/00A61P35/00
Inventor 王文坦黄延宾程易
Owner TSINGHUA UNIV
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