Self-assembled polypeptide, redox response polypeptide hydrogel as well as preparation method and application thereof

A hydrogel and self-assembly technology, applied in chemical instruments and methods, drug combinations, pharmaceutical formulations, etc., can solve problems such as unstable blood circulation, insufficient tissue distribution, etc., achieve low immunogenicity, convenient operation, and shorten the cost. gel time effect

Pending Publication Date: 2021-07-16
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these drug delivery systems can improve drug distribution in tumor tissues through active or passive ta

Method used

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  • Self-assembled polypeptide, redox response polypeptide hydrogel as well as preparation method and application thereof
  • Self-assembled polypeptide, redox response polypeptide hydrogel as well as preparation method and application thereof
  • Self-assembled polypeptide, redox response polypeptide hydrogel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for preparing a redox-responsive polypeptide hydrogel is carried out in two steps.

[0027] 1. Polypeptide synthesis

[0028] (1), swelling of RINK resin

[0029] The RINK resin was placed in a 150ml reactor and soaked with dichloromethane (DCM). After 2 hours, the resin was washed with nitrogen-dimethylformamide (DMF) 3 times the volume of the resin, and then sucked dry for use.

[0030] (2) Remove the Fmoc protecting group

[0031] A certain amount of 20% piperidine was added into the reactor to remove the Fmoc protecting group on the resin. After deprotection, wash with 3 times the resin volume of DMF, and then drain.

[0032] (3) Activation and coupling of amino acids

[0033] The amino acid to be coupled is activated using the activating agent HOBT, which is added to the reactor for coupling.

[0034] (4) Peptide chain extension

[0035] According to the amino acid sequence, the above steps of deprotection and coupling are repeated, and the correspo...

Embodiment 2

[0045] The synthesis method of the polypeptide is the same as in Example 1. Dissolve the synthesized peptide in 0.1% HO 2 o 2 Solution, vortex until dissolved to form a peptide solution with a concentration of 15 mg / ml, and stand at room temperature to form a redox-responsive polypeptide hydrogel.

Embodiment 3

[0047] The synthesis method of the polypeptide is the same as in Example 1. Dissolve the synthesized peptide in 0.1% HO 2 o 2 Solution, vortex until dissolved to form a peptide solution with a concentration of 20 mg / ml, and stand at room temperature to form a redox-responsive polypeptide hydrogel.

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PUM

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Abstract

The invention relates to a redox response polypeptide hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. The invention provides a polypeptide that can be self-assembled to form hydrogel, the polypeptide is dissolved in water and then forms a redox response hydrogel under crosslinking of H2O2. The hydrogel has a compact fiber network structure, relatively strong mechanical properties and good biocompatibility. The hydrogel has high sensitivity to glutathione (GSH), and the GSH responsive release of the drug can be realized by loading the doxorubicin hydrochloride into the hydrogel. The drug-loaded hydrogel is implanted into a tumor part through injection, so that the anti-tumor effect of the drug can be improved, and the toxic and side effects of the drug on normal tissues are effectively reduced.

Description

technical field [0001] The invention belongs to biomedical material technology, and in particular relates to a redox responsive polypeptide hydrogel, a preparation method thereof, and an application as a drug loading material. Background technique [0002] Cancer is a serious threat to human life and health, chemotherapy is still one of the most effective treatment methods. Common anticancer drugs such as anthracyclines, metal platinum complexes, taxanes and camptothecins can effectively inhibit the growth of cancer cells, but they can also cause damage to normal cells, so their clinical application are greatly restricted. In order to improve the efficacy of anticancer drugs and reduce their side effects, a variety of tumor-targeted nano-drug delivery carriers have been developed, such as liposomes, micelles, dendrimers, etc. Although these drug delivery systems can improve drug distribution in tumor tissues through active or passive targeting, there are still many drawbac...

Claims

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Application Information

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IPC IPC(8): C07K7/02A61K47/42A61K9/06A61K31/704A61P35/00
CPCC07K7/02A61K47/42A61K9/06A61K9/0024A61K31/704A61P35/00
Inventor 葛亮李宜平朱颖
Owner CHINA PHARM UNIV
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