57 results about "Doxorubicin hcl" patented technology

The invention belongs to a method for preparing composite micro-spheres with Fe3O4@C core-shell structures and application of the composite micro-spheres. The method includes preparing Fe2O3 nano-particles by the aid of modified solvothermal processes; preparing morphologically controllable composite micro-spheres with Fe2O3@ppy core-shell structures by the aid of combinations of template processes, hydrothermal processes and ultrasonic assisting processes without optional surfactants under mild conditions; reducing the morphologically controllable composite micro-spheres with the Fe2O3@ppy core-shell structures under the protection of nitrogen atmosphere to obtain the composite micro-spheres with the Fe3O4@C core-shell structures and superparamagnetism. The method and the application have the advantages that doxorubicin hydrochloride is used as a medicine model to carry out medicine loading and release tests, and the composite micro-spheres are excellent in medicine sustained-release and controlled-release performance as shown in the tests; the composite micro-spheres with the magnetic composite F3O4@C core-shell structures has a sustained-release function and the superparamagnetism which are integrated with each other, and accordingly the composite micro-spheres can have broad application prospects in the biomedical field of targeted therapy and the like.

The invention provides pH-sensitive doxorubicin hydrochloride loaded silver nano-cluster hydrogel. A preparation method of the silver nano-cluster hydrogel comprises the following steps: (1), an AgNO3 solution is prepared, placed in a microwave reactor, stirred and heated, a cysteine solution is added dropwise, and a silver nano-cluster dispersing agent is prepared through reaction; (2), a citric acid solution is added to the silver nano-cluster dispersing agent, the mixture is placed on a small shaking table and subjected to ultraviolet irradiation, an NaHCO3 solution is added to regulate pH after the solution is transparent, high-speed centrifugation is performed, doxorubicin hydrochloride powder is added, the mixture is placed in a high-pressure reactor, nitrogen is introduced, the pressure and the temperature in the reactor are adjusted, red and clear jelly, namely, the pH-sensitive doxorubicin hydrochloride loaded silver nano-cluster hydrogel, is obtained through reaction. The silver nano-cluster hydrogel is taken as a carrier to coat doxorubicin hydrochloride and is injected to a tumor position through orthotopic injection, the pH of the tumor position is responded, so that slow drug release is realized, the tumor inhibition rate is increased, and cardiac toxicity is reduced.

The invention relates to a preparation method of amycin controlled-release chitosan nano particles with pH / oxido-reduction dual response. Thiolated chitosan, carboxymethyl chitosan, sodium tripolyphosphate, doxorubicin hydrochloride and hydrogen peroxide are taken as raw materials. The preparation method comprises the following steps: mixing a thiolated chitosans solution with a doxorubicin hydrochloride solution so as to obtain a solution I; mixing a carboxymethyl chitosan solution with a sodium tripolyphosphate solution so as to obtain a mixed solution II; dropwise adding the mixed solution I into the mixed solution II while stirring, controlling the dosage of carboxymethyl chitosan and thiolated chitosan, regulating the ph value of the solution to 6-8, performing ultrasonic blending, performing ion crosslinking and polymer condensing, then dropwise adding hydrogen peroxide into the system so as to finish oxidization crosslinking, performing room-temperature stirring reaction, separating products, and drying the products to obtain the amycin controlled-release chitosan nano particles with pH / oxido-reduction dual response. The amycin controlled-release chitosan nano particles with pH / oxido-reduction dual response is capable of escaping away from tumor cell endosome / lysosome, is capable of automatically regulating and controlling to realize cytoplast release based on unique microenvironments of tumor cells, improving the amycin medicine delivery efficiency and the medical effect, and has a favorable research and development application background in multiple aspects such as medicine and medical materials.

The invention discloses a dual-signal electrochemical sensor for detecting a doxorubicin hydrochloride rate based on a bare glassy carbon electrode. A specific detection method comprises the steps of immersing the glassy carbon electrode into phosphatic water buffer solution containing DOX and methylene blue (MB), correlating a ratio delta IDOX / delta IMB between the electrochemical signal intensities of two substrates with the concentration of the DOX, and drawing a linear standard curve; measuring an SWV signal of a sample containing the DOX and the MB and determining the content of the DOX in serum according to the drawn standard curve. The prepared dual-signal electrochemical sensor for detecting the DOX rate based on the bare glassy carbon electrode is simple to operate, high in flexibility and good in stability and has important significance in detecting the dosage of the DOX in the serum of a cancer patient.

The invention discloses a preparation method of antigen determinant / doxorubicin hydrochloride (DOX) double-template molecularly imprinted fluorescent nanoparticles with an alpha-helix structure. Fluorescent nano silicon spheres wrapping silicon nano-particles are used as a carrier, P32 protein N-terminal conformation 9 peptides and doxorubicin hydrochloride are used as double-template molecules, and the double-molecular imprinted nano particles are prepared by adopting a precipitation polymerization method. According to the invention, the Si nano particles are wrapped in silicon dioxide to prepare fluorescent nano silicon spheres and the fluorescent double-template molecularly imprinted nano particles, and the fluorescence characteristic can be used for fluorescence imaging of tumor cells.A polypeptide with an alpha-helix structure constructed by using trifluoroethanol is used as a template molecule, so that target identification of target tumor cells can be effectively improved. Thenano particles are prepared by adopting the conformation polypeptide and the doxorubicin hydrochloride as double templates, targeted medicine delivery is realized while targeted specific recognition of tumor cells is realized, the amount of medicines reaching target sites is increased, side effects are reduced, the medicine utilization rate is improved, and the treatment effect is enhanced.

The invention relates to microspheres with a liver active targeting function and pH and reduction stimulative responsiveness, a preparation method thereof, and an application of the microspheres in medicine. The microspheres are characterized in that: (1) a carrier is composed of galactose modified gelatin and poly(beta-amino ester), and the microspheres are prepared through an emulsion cross-linking method with galactose modified gelatin and linear poly(beta-amino ester) containing a disulfide bond as monomers, and dialdehyde as a crosslinking agent, wherein a terminal group of the linear poly(beta-amino ester) is a secondary amino group; (2) the drug-loaded microspheres are prepared through the following steps: with an antineoplastic drug, the galactose modified gelatin and the poly(beta-amino ester) as components, dispersing doxorubicin hydrochloride as a model drug of the antineoplastic drug into the monomers, i.e., the galactose modified gelatin and the linear poly(beta-amino ester), then adding the crosslinking agent, and subjecting the two monomers to a crosslinking reaction so as to form the microspheres; and (3) the prepared microspheres have good recognition properties to liver cells and good pH and reduction stimulative release properties to model drugs, and can be further researched and developed into a novel targeting preparation used for treatment of liver cancer.

This invention is in the field of chemical restructuring of pharmaceutical agents known to cause tissue toxicity as a side effect, by producing their orotate derivatives. More particularly, it concerns orotate derivatives of the anthracyclines, doxorubicin and daunorubicin, that are found to reduce levels of the pharmaceutical agent in noncancerous tissues. There orotate derivatives are equally efficacious in inhibiting the SCCAKI-1 kidney tumor in animals and the reduction in the heart tissue of doxorubicin compared with doxorubicin HCl suggests a reduction in toxicity induced by free radical generation by the anthrracyclines.

The invention discloses a modified carboxymethyl chitosan microsphere as well as a preparation method and application thereof. The preparation method comprises the following steps of firstly, preparing poly(N-acryloyl taurine), then adding the poly(N-acryloyl taurine) into a carboxymethyl chitosan solution to form a mixed solution, and finally, preparing the carboxymethyl chitosan and poly(N-acryloyl taurine) interpenetrating network microsphere by a suspension crosslinking method. According to the preparation method provided by the invention, the poly(N-acryloyl taurine) is formed by polymerizing monomer N-acryloyl taurine; the density of sulfonic acid groups is relatively high; in a structural unit of the poly(N-acryloyl taurine), negative charge carried by the sulfonic acid groups of side chains interact with drug molecules with positive charge; and the groups are introduced into an embolism microsphere, so that the drug loading rate of the microsphere on an anti-cancer drug doxorubicin hydrochloride can be obviously improved.

The invention provides a sulfydryl-containing zwitterionic polypeptide modified doxorubicin derivative, a nano-micelle and a preparation method of the nano-micelle, and belongs to the field of biological medicines. The doxorubicin derivative has a structure as shown in a general formula (I). The preparation method of the doxorubicin derivative comprises the following steps of in a polar solvent, mixing sulfydryl-containing zwitterionic polypeptide with acryloylhydrazine to react, and performing crystallizing to obtain a polypeptide derivative; and dissolving the polypeptide derivative and doxorubicin hydrochloride in the polar solvent according to a molar ratio of 1: (0.2-5), adding a trifluoroacetic acid catalyst, and carrying out a stirring reaction to obtain the doxorubicin derivative.The nano-micelle prepared from the doxorubicin derivative is long in in-vivo blood circulation time, high in drug loading capacity, small in toxic and side effects, good in pH value response and goodin tumor inhibition effect.

The invention discloses a pH-response drug-loaded self-assembly aquagel-formed polypeptide, a preparation method and use. An amino acid sequence of the polypeptide is Ile-Glu-Ile-Ile-Ile-Orn. Aquagel is formed through dissolving the polypeptide in an aqueous solution, carrying out swirling until the polypeptide is dissolved so as to form a polypeptide solution, adjusting a pH, and carrying out standing gelatination at room temperature. The obtained polypeptide aquagel is good in biocompatibility, high in mechanical properties and low in toxic or side effects; and the polypeptide aquagel disclosed by the invention has relatively high sensitivity to the pH, the aquagel loading doxorubicin hydrochloride is implanted to a focus region in an injection manner, the drug-loaded polypeptide aquagel can achieve targeted sustained / controlled release of a drug under the stimulation of an acidulous environment of a tumor, the antitumor treatment effect of the drug is improved, and the drug-loaded polypeptide aquagel is free of toxic or side effects on normal tissue.

The invention discloses erythrocyte membrane coated drug-loaded nanoparticles / probes and application thereof in diagnosis and treatment of glioblastoma. The erythrocyte membrane coated drug-loaded nanoparticles are obtained by coating an erythrocyte membrane of a human or animal with doxorubicin hydrochloride-loaded poly(lactic-co-glycolic acid) (PLGA). The nanoparticles obtained by the invention have the characteristics of similar particle size and shape, capability of providing a relatively stable internal environment, long circulation time in blood in an animal body, biocompatibility, high targeted accumulation content in a tumor and the like, tumor diagnosis can be realized through fluorescence imaging, and a drug can be released at a tumor part; tumor growth is inhibited in vivo, and the survival time of animals is prolonged.

The invention discloses a preparation method of zein / calcium carbonate composite particles. The preparation method comprises the following steps of sufficiently dispersing the zein in 70%-90% alcohol liquor, adding calcium chloride and stirring and dissolving to form mixed liquor of calcium chloride and the zein, then, adding sodium carbonate liquor with amount of substance equal to that of calcium chloride, stirring at a high speed until the liquor is light blue; and then, keeping final concentration of the zein to 0.1g.L<-1> to 10 g.L<-1> by controlling addition amount of the zein, dialyzing and purifying to obtain the zein / calcium carbonate composite particles. Controlled release performance experiments of doxorubicin hydrochloride indicate that the zein / calcium carbonate composite particles have very good medicine carrying performance and controlled release performance to water-soluble small molecule drugs, and therefore, the zein / calcium carbonate composite particles can be used as water-soluble small molecule drug carriers for being used for producing pharmaceutical preparations.

The invention discloses a preparation method of antigen determinant / doxorubicin hydrochloride (DOX) double-template molecularly imprinted fluorescent nanoparticles with an alpha-helix structure. Fluorescent nano silicon spheres wrapping silicon nano-particles are used as a carrier, P32 protein N-terminal conformation 9 peptides and doxorubicin hydrochloride are used as double-template molecules, and the double-molecular imprinted nano particles are prepared by adopting a precipitation polymerization method. According to the invention, the Si nano particles are wrapped in silicon dioxide to prepare fluorescent nano silicon spheres and the fluorescent double-template molecularly imprinted nano particles, and the fluorescence characteristic can be used for fluorescence imaging of tumor cells.A polypeptide with an alpha-helix structure constructed by using trifluoroethanol is used as a template molecule, so that target identification of target tumor cells can be effectively improved. Thenano particles are prepared by adopting the conformation polypeptide and the doxorubicin hydrochloride as double templates, targeted medicine delivery is realized while targeted specific recognition of tumor cells is realized, the amount of medicines reaching target sites is increased, side effects are reduced, the medicine utilization rate is improved, and the treatment effect is enhanced.