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Erythrocyte membrane coated drug-loaded nanoparticles/probes and application thereof in diagnosis and treatment of glioblastoma multiforme

An erythrocyte membrane, drug-loaded nanotechnology, which is used in medical preparations with non-active ingredients, medical preparations containing active ingredients, drug combinations, etc., to achieve the effects of being difficult for immune system clearance, prolonging systemic action time, and inhibiting tumor growth.

Pending Publication Date: 2021-10-15
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the treatment of glioblastoma with red blood cell membrane-coated drug-loaded nanoparticles / probes

Method used

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  • Erythrocyte membrane coated drug-loaded nanoparticles/probes and application thereof in diagnosis and treatment of glioblastoma multiforme
  • Erythrocyte membrane coated drug-loaded nanoparticles/probes and application thereof in diagnosis and treatment of glioblastoma multiforme
  • Erythrocyte membrane coated drug-loaded nanoparticles/probes and application thereof in diagnosis and treatment of glioblastoma multiforme

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Experimental program
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preparation example Construction

[0040] The invention provides a preparation method of erythrocyte membrane-wrapped drug-loaded nanoparticles and its application in the diagnosis and treatment of glioblastoma; comprising:

[0041] (1) Extract human or animal red blood cell membranes by using the hypotonic membrane breaking method, and dissolve doxorubicin hydrochloride and polylactic acid-glycolic acid copolymer into the acetone solution by using the nanoprecipitation method, and absorb the volatilized acetone through a rotary evaporator, Prepare translucent uniform drug-loaded nanoparticles with carboxyl groups on the surface. Utilizing the hydrophilic and hydrophobic interactions between the phospholipid bilayer of red blood cell membrane and membrane protein and carboxylated drug-loaded nanoparticles, the red blood cell membrane is coated on the nanoparticles by ultrasonic method. On the surface, the red blood cell membrane-coated drug-loaded nanoparticles were prepared.

[0042] (2) Characterize the physi...

Embodiment 1

[0048] Embodiment 1, preparation of erythrocyte membrane-coated drug-loaded nanoparticles

[0049] (1) Use isotonic centrifugation and hypotonic membrane rupture to extract human or animal red blood cell membranes: add 1mM EDTANa2 and heparin sodium 500U / L to 10mL phosphate buffer in sequence, mix well and add 2mL fresh human or animal peripheral blood, five Sub-low-speed centrifugation and high-speed centrifugation to discard the supernatant to obtain precipitated red blood cells, dilute to 2 mL with phosphate buffer, add 7.6 mL of 0.25 mMEDTANa2 solution and vortex for 5 minutes, add 380 μL of 20x phosphate buffer, centrifuge and discard the supernatant, repeat three times The post-precipitation was dispersed with 0.25mM EDTANa2 solution to obtain 2mL red blood cell membrane suspension.

[0050] (2) Preparation of polylactic-co-glycolic acid (PLGA-DOX) nanoparticles loaded with doxorubicin hydrochloride: 20 mg of PLGA was dissolved in 1 mL of acetone solution, and ultrasonic...

Embodiment 2

[0052] Example 2, Characterization of Erythrocyte Membrane Coated Drug-loaded Nanoparticles

[0053] (1) Take an appropriate amount of PLGA-DOX-RBC-SL1 nanoparticle solution, and 2 After diluting O, ultrasonically disperse, drop a little (10 μL) on a copper grid with a support membrane (drip net method), let it stand for 5 minutes, and use a small piece of filter paper to blot the excess liquid on the edge of the copper grid. After drying, negatively stain with 1% phosphotungstic acid for 10 minutes, and then use a small piece of filter paper to blot the excess liquid on the edge of the copper mesh. After standing under the infrared lamp and fully drying, the size and shape of the nanoparticles were observed under a transmission electron microscope (TEM), and the average diameter of the nanoparticles PLGA-DOX-RBC-SL1 was calculated by measuring the diameter of 100 particles. particle size.

[0054] (2) Take an appropriate amount of nanoparticle (PLGA-DOX-RBC-SL1) solution an...

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Abstract

The invention discloses erythrocyte membrane coated drug-loaded nanoparticles / probes and application thereof in diagnosis and treatment of glioblastoma. The erythrocyte membrane coated drug-loaded nanoparticles are obtained by coating an erythrocyte membrane of a human or animal with doxorubicin hydrochloride-loaded poly(lactic-co-glycolic acid) (PLGA). The nanoparticles obtained by the invention have the characteristics of similar particle size and shape, capability of providing a relatively stable internal environment, long circulation time in blood in an animal body, biocompatibility, high targeted accumulation content in a tumor and the like, tumor diagnosis can be realized through fluorescence imaging, and a drug can be released at a tumor part; tumor growth is inhibited in vivo, and the survival time of animals is prolonged.

Description

technical field [0001] The invention belongs to the field of molecular imaging and biotechnology, relates to nano-medicine for tumor diagnosis and treatment, in particular to a red blood cell membrane-encapsulated drug-loaded nanoparticle / probe, a preparation method thereof, and an application in the diagnosis and treatment of brain glioblastoma. Background technique [0002] Glioblastoma, the most common primary brain tumor, is one of the most difficult to treat malignancies due to its infiltrative growth characteristics. According to data, the 1-year survival rate of glioblastoma after diagnosis is only about 30%, and the average survival period is only 53 weeks. The average survival period is only 19.6 months. Due to the existence of the blood-brain barrier (BBB), 98% of small molecular compounds and almost all macromolecular substances cannot reach the site of glioma, which makes early diagnosis difficult and limits the choice of chemotherapy drugs . [0003] In recen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K31/704A61K47/34A61K47/46A61K47/54A61K47/69A61K49/00A61P35/00
CPCA61K9/5176A61K9/5153A61K31/704A61K47/549A61K47/6925A61K49/0052A61K49/005A61K49/0054A61P35/00
Inventor 张军刘先平王剑虹庞志清耿道颖
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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