Bosea sp. AS-1 strain capable of tolerating pollution of arsenic and antimony and oxidizing As(III) and applications thereof
A technology of Baoziella and AS-1, applied in the field of microorganisms, can solve the problems of high cost, secondary pollution, strain tolerance concentration and low oxidation efficiency, and achieve the reduction of arsenic hazards, strong oxidation and adaptability , the effect of strong mobility
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[0010] The present invention will be described in detail below in conjunction with specific examples, but the protection scope of the present invention is not limited to the following examples.
[0011] Screening, isolation and identification of strains
[0012] In this embodiment, add a certain concentration of NaAsO from the surface soil polluted by arsenic in Xikuangshan, Hunan Province, China. 2 Carry out enrichment culture, then dilute the enriched cultured soil samples and coat with a certain concentration of NaAsO 2 Arsenic-resistant bacteria were grown on the MMM solid medium plate, and colonies of different shapes were picked and streaked to obtain a single colony, and then inoculated into MMM liquid medium, and KMnO 4 The arsenic-oxidizing bacteria were detected by the method, and the concentrations of As(Ⅲ) and As(Ⅴ) were measured by high-performance liquid chromatography-hydride generation-atomic fluorescence (HPLC-HG-AFS), and the oxidation rate was calculated. ...
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