63results about How to "Low denaturation" patented technology

The invention discloses an extracting method for a sturgeon skin collagen. The extracting method comprises the following steps: (1) preliminary treatment: taking and slicing a sturgeon skin, firstly immersing the sturgeon skin in alkaline solution, immersing the sturgeon skin in an acidic solution after rinsing, and then taking the sturgeon skin out for rinsing and standby; (2) extraction: adding water into the preprocessed sturgeon skin for leaching at 45 to 55 DEG C, and carrying out the filtration to remove impurities and obtain the collagen extracting solution; and (3) freeze drying: obtaining sturgeon skin collagen powder through the refrigerated centrifugation of the collagen extracting solution. The collagen product has higher quality. The extracting method has a higher collagen extraction rate, and not only improves the product yield, but also saves the cost in a simple way, thereby improving the economic benefits of a manufacturing enterprise.

The invention belongs to the fields of soybean protein deep processing and functional beverage, and discloses acid-soluble soybean protein and a preparation method and application of the acid-soluble soybean protein in acid beverage. The method comprises the following steps: firstly, mixing extract liquid with bean pulp, centrifugally separating and removing insoluble residues; taking supernatant liquid, adding phytase to the supernatant liquid to carry out enzymolysis, and adjusting the pH value; and carrying out cyclic ultrafiltration percolation concentration under a water bath condition, repeatedly diluting and concentrating protein for a plurality of time, and carrying out pasteurization and spray drying on the concentrated protein liquid, so as to obtain an acid-soluble soybean protein product. The purity of the obtained protein can be 90%-99%; the content of phytic acid is only 0.665%-0.694%; the nitrogen solubility index within the pH being 3.0-4.0 is 45%-90%; enzymatic hydrolysate is relatively good in foaming property, relatively excellent in foam stability; the acid-soluble soybean protein can be applied to preparation of various functional beverage when being dissolved into sports beverage, carbonated beverage, fruit juice and the like.

The invention relates to a backward extraction method for extracting rapeseed protein by reverse micelle, which has simple process, great reduction of protein denaturation degree, high protein extraction efficiency as well as obvious loss reduction of a surface active agent, and can save water resources and reduce energy consumption in industrial production. The backward extraction method for extracting rapeseed protein by reverse micelle comprises the following steps: using a reverse micelle solution to extract rapeseed protein; then obtaining a forward extraction solution; carrying out vacuum concentration on the forward extraction solution; adding a buffer solution after an organic solvent in the reverse micelle solution is removed; then carrying out extraction separation, thereby obtaining an aqueous solution of the rapeseed protein. A method for extracting rapeseed protein by a reverse micelle solution comprises the following steps: adding rapeseed powders into the reverse micelle solution, extracting the rapeseed powders under the action of ultrasonic waves, and then obtaining the forward extraction solution by solid-liquid separation. The vacuum concentration method uses rotary evaporation, and water bath temperature is controlled between 50 DEG C and 70 DEG C. The invention has simple process, resource saving, low production cost, mild operating conditions and high protein extraction efficiency.

The invention discloses a method for extracting soybean oil and proteins and belongs to an extraction processing technology of plant oil and proteins. The method comprises the following steps: (1) cleaning and grinding soybeans, mixing the soybeans with water to form a mixed solution, adding protease into the mixed solution for performing ultrasonic-assisted enzymolysis treatment, and performing centrifugal separation to obtain soybean oil, emulsion, hydrolysate and residues; (2) adding a reverse micelle system into the emulsion and the hydrolysate for performing high-pressure reverse micelle extraction, performing back extraction on the extracting solution, and performing centrifugal separation to obtain a surfactant phase, an organic phase and an aqueous phase; (3) performing rotary evaporation on the organic phase to obtain the soybean oil, performing ultrafiltration on the aqueous phase, and performing vacuum drying and freeze drying to obtain the soybean protein. According to the method, the ultrasonic technology, the enzymolysis technology, the high-pressure technology and the reverse micelle extraction technology are organically combined together, the method is applied to extraction processing of the soybean oil and proteins, the extraction efficiency is effectively improved, the prepared soybean oil is high in quality, and high-purity low-degeneration soybean proteins can be obtained.

The invention discloses a method for preparing oil by a low aqueous enzymatic method and organic solvent extraction. The method comprises the following steps of: based on 100 Kg of oil plants, dissolving 0.5 to 3.0 Kg of complex enzyme which comprises cellulolytic enzyme, pectinase, protease, and flavourzyme in a ratio of 2 to 3 to 4 to 3 in 40 to 70L of buffer solution with the pH value of 5.0 to 7.0 to obtain enzyme solution; cleaning the oil plants and removing impurities, crushing and sieving, wherein the particle diameter is 40 to 80 meshes; spraying the enzyme solution onto the crushed oil plants, stirring uniformly, and reacting at the constant temperature of between 40 and 55 DEG C for 2 to 4 hours; placing the reacted materials in a drying oven at the temperature of between 40 and 65 DEG C and drying for 2 days; hardening and tempering the materials to ensure that the moisture content is kept to be 3+/-5 percent; and extracting the dried, hardened and tempered materials by anorganic solvent at the temperature of between 50 and 70 DEG C for 1 to 2 hours to obtain the extraction oil and egg oil material cakes. The method is high in oil yield, and the obtained oil and cake have high quality.

A muscle protein purifying method adjusts the concentrations of KCl, ATP and ammonium sulfate (A.S.) through the influences of different solution mediums on separation and purification and analyzes the dissociation and precipitation law of all fibrillin components and determines the separation and purification conditions of actin, myoballs and paramyosin with the ATPase activity as the protein denaturation indicating parameter. The method has the advantages that compared with a traditional protein purifying method, the novel method is low in purified protein denaturation degree and simple in purifying process, the actin, myoballs and paramyosin in smooth muscles of aquatic invertebrates are efficiently obtained through purification, and the purifying cost is saved to the maximum extent.

The invention discloses oil-bearing maize embryo albumen powder containing 5-12 percent of oil grease. The preparation of the oil-bearing maize embryo albumen powder is as follows: low-modified oil-bearing corn germ meal is obtained with fresh maize embryo through low-temperature drying, roasting and squeezing; the oil-bearing maize embryo albumen powder is prepared by taking the obtained oil-bearing corn germ meal as raw material through an alkaline extraction and acid precipitation process. The albumen powder is rich in nutrition and good in quality and can be widely used in the food service industry. The invention also discloses a method for preparing the oil-bearing maize embryo albumen powder; which can greatly reduce the modification degree of the maize embryo protein in the processing and significantly increase the rate of comprehensive utilization and the added economic value of the maize embryo under the condition of low cost.

The invention relates to a Chinese medicine with the functions of eliminating lipid and removing heat from the liver and a preparation method thereof. The Chinese medicine is a preparation prepared by giant knotweed, hawthorn, rhubarb, herba artemisiae capillaris and radix bupleuri in proportion. The preparation method is as follows: 1) giant knotweed, hawthorn and rhubarb are taken and extracted with ethanol in a fractionated manner, the extract is merged, the dreg mixture of the three raw materials is separated out by filtering, and after ethanol is recovered, the filtrate is concentrated into the thick extract of the three raw materials; 2) herba artemisiae capillaris and radix bupleuri are taken and merged with the dreg mixture and decocted with distilled water in a fractionated way, decoction is merged and filtered, and the obtained filtrate is concentrated into the thick extract of the five raw materials: 3) the thick extract of the three raw materials and the thick extract of the five raw materials are merged, treated with spray drying, pulverized into fine powder and prepared into pulvis, granules, tablets or capsules. The Chinese medicine can alleviate hyperlipaemia, reduce fat accumulation in the liver and fatty degeneratiaon degree, protect liver function and maintain energy equilibrium of the body, and has the advantages of definite curative effect, few toxic and side effects and safe and reliable performance.

The invention discloses a method for improving the functional characteristics of rice glutenin through phosphorylation treatment, which comprises the following steps: rice glutenin is extracted from broken rice through alkali extraction and acid precipitation, the rice glutenin is mixed with phosphorylating reagent sodium tripolyphosphate and pH value is adjusted. Reaction is carried out at a certain temperature for a period of time, phosphorylated glutenin is obtained through centrifugation, washing, vacuum freeze-drying and other steps. The protein solubility of the phosphorylated rice glutenin is 90%, the emulsifying ability is 20m2/g, and the emulsifying stability is 80%, the foaming ability is 10%, and foam stability is 90%. The method has the advantages of simple production process,low cost, and easy achievement of industrial production, greatly improves the utilization rate and added value of the rice glutenin, and has wide application prospect.

The invention relates to a process for extracting rapeseed protein. The process is simple, and has the advantages that the degree of protein denaturation is greatly reduced, the protein extraction rate is high, and the loss of a surfactant is remarkable reduced; besides, water resources are saved, and energy consumption in industrial production is lowered. In the process for extracting the rapeseed protein by use of reverse micelle, a method for backward extraction comprises the following steps: extracting the rapeseed protein by use of a reverse micelle solution to obtain a forward extraction solution; conducting vacuum concentration on the forward extraction solution to remove an organic solvent in the reverse micelle solution; then, adding a buffer solution for extraction and separation, so as to obtain an aqueous solution of the rapeseed protein. A method for extracting the rapeseed protein by use of the reverse micelle solution comprises the following steps: adding powder of rapeseed dregs into the reverse micelle solution; carrying out extraction under the ultrasonic action; then, carrying out solid-liquid separation to obtain the forward extraction solution. According to the process, vacuum concentration is conducted through rotary evaporation, and the water bath temperature is controlled to be 50-70 DEG C; the process is simple, lower in resource consumption, low in production cost, mild in operating condition, and high in protein extraction rate.

The invention discloses a high oil plant loading double-helix oil press comprising a feeding mechanism, a torque divider, a double-helix shaft, a press cage, a cake output mechanism and an oil discharging mechanism. The torque divider is connected with a driving motor via a speed reducer, the double-helix shaft comprises an upper spindle and a lower spindle which are mutually matched and provided with a plurality of press whorls and conical rings, an upper stop ring and a lower stop ring are respectively arranged on the upper spindle and the lower spindle at feed inlet ends of the double-helix shaft, the press cage is sleeved on the double-helix shaft and forms a press barrel with the same, two gear shafts of the torque divider are respectively connected with the upper spindle and the lower spindle of the double-helix shaft, and an discharge outlet of the feeding mechanism is connected with a feed inlet of the double-helix shaft. By the aid of the high oil plant loading double-helix oil press, oil in oil plants can be pressed out to the greatest extent, industrialization requirements on low oil pressing temperature, high oil output and low albuminous degeneration degree are met, and a base for follow-up full utilization of albumen is established.

The invention discloses a method for extracting protein from soybean meal by adopting an ultrasonic-assisted reverse micelle extraction process, and belongs to plant protein extraction and processing technologies. The method comprises the following steps: (1) grinding defatted soybean meal, screening, mixing with water to form a mixed solution, adding cellulase and protease into the mixed solution in sequence, performing step-by-step enzymatic hydrolysis, and after the enzymatic hydrolysis, performing centrifugal separation to obtain enzymatic hydrolysis liquid and deposits; (2) adding a reverse micelle system into the enzymatic hydrolysis liquid for performing ultrasonic-assisted reverse micelle extraction to obtain extracting liquid, performing back extraction on the extracting liquid, and performing the centrifugal separation to obtain an organic phase and a water phase; (3) performing alkali-solution and acid-isolation on the water phase to obtain deposits, adding the water into the deposits for redissolving, ultra-filtering, concentrating in vacuum and freeze-drying to obtain the soybean protein. According to the method, required process equipment is simple and safe to operate; an ultrasonic technology and a reverse micelle extraction technology are combined for extracting the protein from the soybean meal, so that the protein extraction rate is increased and the extraction cost is reduced; the prepared soybean protein is high in purity, low in modification degree and good in functionality.

The invention relates to a preparation method of low-heat dried skim milk. An object of the invention is to make up disadvantages in production of the low-heat dried skim milk in China and change a situation that the low-heat dried skim milk is depended on import at present. The method achieves a sterilization effect by combining bactofugation and low temperature sterilization. During the whole production process, cow's milk is heated to a temperature no higher than 80 DEG C, so that denaturation level of whey protein is low; a cooking flavor is small; solubility is good; and nutritional value of the milk is high. The obtained dried milk can be used for preparing ice cream, yoghourt, cheese, etc.

The invention relates to rape seed making oil technical field, which is a rape seed extruding processing method and equipment in the procession of making oil. Its main technical characteristic is that the best parameters of rape seed extruding device and press processing system are supplied, making the remainder rate is not larger then 1.3%, without the steps and equipments of softening, squeezing and cooking in conventional processing technique, the steps and equipments of softening, squeezing, injecting steam and cooling water into the extruded material and drying in the preprocessing technique of extruding in nation and abroad, and that of cold squeezing and injecting steam into the extruded material, which makes rape seed stay shorter in pressing cavity, the pressing temperature is not higher then 80-110deg.C, the level of protein degeneration is low, the quality of oil is grad, the an of protein in rape seed is high and each sleeve and of extruding device can be separated along the animal direction.

The invention discloses a preparation method for high-gel-property plasma protein powder. The preparation method comprises the following steps: the step (1) of blood sampling; the step (2) of static separation: stewing the anticoagulant blood for 3-8 h in 4-8 DEG C; the step (3) of device separation: centrifugally separating supernatant liquid of the stewing blood to obtain plasma liquid; the step (4) of membrane concentration: performing the concentration desalting to the plasma liquid by using an ultrafiltration method, and performing the nano-filtration concentration treatment to ultrafiltration penetrated liquid by using a nano-filtration method, and mixing the ultrafiltration concentrated solution and the nano-filtration concentrated solution according to the proportion, wherein the proportion is 1-7:1, the ultrafiltration pressure is 0.4-0.6 MPa, the circulation ultrafiltration time is 8-25 min, and the nano-filtration pressure is 0.1-0.3 MPa; and the step (5) of drying. The invention discloses the high-gel-property plasma protein powder. The color of a prepared plasma protein powder product is faint yellow, the solubility is good, the albuminous degeneration degree is small, the gel property is strong, and the hardness is improved without the peculiar smell.

The invention provides a method for extracting soybean oil through enzymolysis and cold pressing and belongs to the field of food processing. The method comprises steps as follows: (1) raw material treatment: soybean is put into a drying oven to be dried, then crushed, dehulled, crushed again and screened with a standard sieve for standby use, and the water content rate of the soybean is determined; (2) conditioning: drinking water is added to the soybean raw material, so that the mass content of moisture is adjusted to be 12%-20%; (3) treatment and addition of enzymes: the enzymes are weighed in a certain mass proportion, water is added, the mixture is mixed uniformly and preheated in a water bath at the temperature of 42-45 DEG C, the soybean raw material obtained in the step (2) is added, and the mixture is stirred uniformly and left to stand for 4-8 h for standby use; (4) enzymic-method cold pressing: the soybean raw material is put into a cold-pressing expeller, the pressing temperature as well as the rotation speed and the pressure of a screw rod is set, pressing is repeated 2-3 times, pressed oil is collected, and a finished product, namely, soybean oil, is obtained after precipitation. The method has the advantages that the oil extraction rate is increased, the problem of solvent residues in traditional oil extraction is solved, and the safe and nutritional edible oil can be prepared.