Backward extraction method for extracting rapeseed protein by reverse micelle

A technology of rapeseed protein and reverse micelles, which is applied to the preparation method of peptides, chemical instruments and methods, plant peptides, etc., can solve the problems of low protein extraction rate and surfactant loss, and achieve high protein extraction rate and production Low cost and water saving effect

Inactive Publication Date: 2010-01-06
NANJING UNIV OF FINANCE & ECONOMICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this way, not only the protein extraction rate is low, but also a large amount of surfactant is lost.

Method used

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  • Backward extraction method for extracting rapeseed protein by reverse micelle
  • Backward extraction method for extracting rapeseed protein by reverse micelle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Preparation of rapeseed meal powder:

[0036] The high-quality double-low rapeseeds produced in 2008 were selected, hulled on a huller, and air-selected after treatment. The shelled rapeseed is crushed on a hammer cyclone mill, and passed through a 100-mesh sieve after crushing. Use petroleum ether (boiling range of 30-60°C) on a Soxhlet extractor to deoil the crushed rapeseed at 50°C, and place it in a ventilated place for precipitation after treatment, so that the petroleum ether can be completely volatilized. Ventilated and dried again, the gained is the required rapeseed meal powder for the test, and its protein (N×6.25 dry basis) content is measured to be 50.1%.

[0037] (2) Configuration of reverse micellar solution

[0038] Preparation of KCl buffer solution: Weigh 110.8g disodium hydrogen phosphate dihydrate and 9.07g potassium dihydrogen phosphate to prepare 1L of solution, mix 190mL and 10mL respectively, and then add 0.596g potassium chloride to the abo...

Embodiment 2

[0050] (1) Preparation of rapeseed meal powder:

[0051] The high-quality double-low rapeseeds produced in 2008 were selected, hulled on a huller, and air-selected after treatment. The shelled rapeseed is crushed on a hammer cyclone mill, and passed through an 80-mesh sieve after crushing. Use petroleum ether (boiling range of 30-60°C) on a Soxhlet extractor to deoil the crushed rapeseed at 50°C, and place it in a ventilated place for precipitation after treatment, so that the petroleum ether can be completely volatilized. Ventilated and dried again, the gained is the required rapeseed meal powder for the test, and its protein (N × 6.25, dry basis) content is 45.6%.

[0052] (2) Preparation of reverse micellar solution

[0053] KCl buffer solution preparation: prepare 0.2mol L -1 Disodium hydrogen phosphate solution and 0.1mol·L -1 1L of each citric acid solution, take 10.30mL and 9.70mL respectively and mix well. Get 0.1193g potassium chloride again and add in the above-...

Embodiment 3

[0064] (1) Preparation of rapeseed meal powder:

[0065] The high-quality common rapeseeds produced in 2008 were selected for hulling treatment on a huller, and then winnowed after treatment. The shelled rapeseed is crushed on a hammer cyclone mill, and passed through an 80-mesh sieve after crushing. Use petroleum ether (boiling range of 30-60°C) on a Soxhlet extractor to deoil the crushed rapeseed at 50°C, and place it in a ventilated place for precipitation after treatment, so that the petroleum ether can be completely volatilized. Ventilated and dried again, the gained is the rapeseed meal powder required for the test, and its protein (N×6.25, dry basis) content is 43.21%.

[0066] (2) Preparation of reverse micellar solution:

[0067] KCl buffer solution preparation: prepare 50mL 0.05mol L respectively -1 Disodium hydrogen phosphate solution and 4.1mL 0.1mol·L -1 Sodium hydroxide, mix well, add water to dilute to 100mL, and take 1.118g of potassium chloride and add to ...

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Abstract

The invention relates to a backward extraction method for extracting rapeseed protein by reverse micelle, which has simple process, great reduction of protein denaturation degree, high protein extraction efficiency as well as obvious loss reduction of a surface active agent, and can save water resources and reduce energy consumption in industrial production. The backward extraction method for extracting rapeseed protein by reverse micelle comprises the following steps: using a reverse micelle solution to extract rapeseed protein; then obtaining a forward extraction solution; carrying out vacuum concentration on the forward extraction solution; adding a buffer solution after an organic solvent in the reverse micelle solution is removed; then carrying out extraction separation, thereby obtaining an aqueous solution of the rapeseed protein. A method for extracting rapeseed protein by a reverse micelle solution comprises the following steps: adding rapeseed powders into the reverse micelle solution, extracting the rapeseed powders under the action of ultrasonic waves, and then obtaining the forward extraction solution by solid-liquid separation. The vacuum concentration method uses rotary evaporation, and water bath temperature is controlled between 50 DEG C and 70 DEG C. The invention has simple process, resource saving, low production cost, mild operating conditions and high protein extraction efficiency.

Description

technical field [0001] The invention relates to a post-extraction method for extracting rapeseed protein from reverse micelles. Background technique [0002] Rapeseed is an oil crop produced in a large area in my country, and its output ranks first in the world. The amount of by-products produced during the processing of rapeseed oil is huge, and the output of rapeseed meal alone exceeds 10 million tons per year. Rapeseed meal contains 35% to 42% crude protein, and the content of lysine, cystine and methionine in rapeseed protein is relatively high, and the amino acid balance is better than that of soybean protein. It is a source of plant protein. [0003] At present, using rapeseed or rapeseed meal as raw material, the methods for producing rapeseed protein mainly include aqueous phase method, aqueous phase enzymatic hydrolysis method, organic solvent extraction, ultrafiltration, diafiltration and other methods. The aqueous phase method often has a low protein extraction ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C07K1/14
Inventor 王立峰朱均旺袁建鞠兴荣何荣
Owner NANJING UNIV OF FINANCE & ECONOMICS
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