Method for preparing oil by low aqueous enzymatic method and organic solvent extraction

An organic solvent and low moisture technology, applied in the direction of fat oil/fat production, fat production, etc., can solve the problems of difficult emulsion breaking, oil-water separation, large waste water, etc., and achieve the effects of easy refining, less water consumption, and low energy consumption

Inactive Publication Date: 2011-09-14
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the main disadvantage of this method is that the high-moisture enzyme reaction will cause difficulty in oil-water separation and demulsification, and in addition, a large amount of waste water will be generated during the production process.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] A method for preparing oil by low-moisture enzymatic assisted organic solvent extraction, comprising the following steps:

[0014] (1) Preparation of enzyme solution: the ratio of compound enzyme is 2:3:4:3 for cellulase, pectinase, protease and flavor enzyme, dissolve 0.5Kg of compound enzyme into 40L of pH 5.0 buffer Zhongde Enzyme Solution;

[0015] (2) Oil pretreatment: Take 100 Kg of dried rapeseed, clean and remove debris, shriveled seeds, impurities, ash, etc. to obtain 95Kg of essential rapeseed, and crush to a degree of 40 mesh; spray the crushed rapeseed Stir the enzyme solution evenly, and react at a constant temperature of 40°C for 2 hours. Then the reacted material was dried in a drying oven at 60°C for 2 days, and then the material was tempered to keep the moisture content at 3%±0.5%.

[0016] (3) Extraction: Add the dried and tempered material into an extractor at room temperature and extract it with No. 6 solvent, the extraction temperature is 50°C, an...

Embodiment 2

[0018] A method for producing oil by low-moisture enzymatic assisted organic solvent extraction, comprising the following steps:

[0019] (1) Preparation of enzyme solution: the ratio of compound enzyme is 2:3:4:3 for cellulase, pectinase, protease and flavor enzyme, and dissolve 1.5Kg of compound enzyme into 50L of pH 6.0 buffer Zhongde Enzyme Solution;

[0020] (2) Oil plant pretreatment: Take 100Kg of dried camellia oleifera seeds, and after cleaning, remove debris, shriveled seeds, impurities, ash, shells and skins, etc., to obtain 68Kg of essential oil camellia seeds. 68 kg of the above-mentioned essential oil tea seeds were crushed to a degree of 60 mesh, sprayed with an enzyme solution on the crushed tea seeds, stirred evenly, and reacted at a constant temperature of 50° C. for 3 hours. Then the reacted material was dried in a drying oven at 60°C for 2 days, and then the material was tempered to keep the moisture content at 3%±0.5%.

[0021] (3) Extraction: Add the dr...

Embodiment 3

[0023] A method for preparing oil by low-moisture enzymatic assisted organic solvent extraction, comprising the following steps:

[0024] (1) Preparation of enzyme solution: the ratio of compound enzyme is 2:3:4:3 for cellulase, pectinase, protease and flavor enzyme, and dissolve 3Kg of compound enzyme into 70L of pH 7.0 buffer Enzyme solution;

[0025] (2) Oil pretreatment: Take 100Kg of dried peanut kernels, and after cleaning, remove debris, shriveled seeds, impurities, ash, skin, etc., to obtain 98Kg of refined peanut kernels with a moisture content of 6.9%. 98 kg of the above-mentioned refined peanut kernels were crushed to a degree of 40 meshes, sprayed with an enzyme solution on the crushed peanuts, stirred evenly, and reacted at a constant temperature of 55° C. for 4 hours. Subsequently, the reacted material was dried in a drying oven at 60°C for 2 days, and then the material was tempered to keep the moisture content at about 3%±0.5%.

[0026] (3) Extraction: Add the...

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PUM

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Abstract

The invention discloses a method for preparing oil by a low aqueous enzymatic method and organic solvent extraction. The method comprises the following steps of: based on 100 Kg of oil plants, dissolving 0.5 to 3.0 Kg of complex enzyme which comprises cellulolytic enzyme, pectinase, protease, and flavourzyme in a ratio of 2 to 3 to 4 to 3 in 40 to 70L of buffer solution with the pH value of 5.0 to 7.0 to obtain enzyme solution; cleaning the oil plants and removing impurities, crushing and sieving, wherein the particle diameter is 40 to 80 meshes; spraying the enzyme solution onto the crushed oil plants, stirring uniformly, and reacting at the constant temperature of between 40 and 55 DEG C for 2 to 4 hours; placing the reacted materials in a drying oven at the temperature of between 40 and 65 DEG C and drying for 2 days; hardening and tempering the materials to ensure that the moisture content is kept to be 3+/-5 percent; and extracting the dried, hardened and tempered materials by anorganic solvent at the temperature of between 50 and 70 DEG C for 1 to 2 hours to obtain the extraction oil and egg oil material cakes. The method is high in oil yield, and the obtained oil and cake have high quality.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an enzyme-assisted oil production method. Background technique [0002] At present, the methods of extracting oil mainly include organic solvent extraction and aqueous enzymatic pretreatment oil extraction. The main method of the organic solvent extraction method is: after the oil is cleaned, crushed, and sieved, it is added to petroleum ether or No. 6 solvent, extracted for a period of time with an extractor, ultrasonic wave, etc., and then refined through deacidification, deodorization, etc. to obtain edible oil. This method is convenient to use and easy to operate, but it also has disadvantages such as relatively long oil extraction time, low oil extraction efficiency, and large solvent consumption. Aqueous enzymatic pretreatment oil extraction method is an enzyme preparation that can degrade vegetable oilseed cell walls, lipid bodies and their complexes (lipoprotein,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/10C11B1/04
Inventor 邱树毅周鸿翔刘林
Owner GUIZHOU UNIV
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