Method for extracting soybean oil and proteins

A soybean oil and protein technology, which is applied in the field of extraction and processing of vegetable oil and protein, can solve the problems of difficult research, low oil yield, difficult emulsion separation and other problems, and achieve simple process equipment, oil extraction rate and protein Improvement of extraction rate and better quality of soybean oil

Inactive Publication Date: 2014-08-27
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
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Problems solved by technology

This technology has mild treatment conditions and a simple process route, but it is also a difficult process to study, especially for oils with low oil content, the oil yield is low, and the emulsion formed by protein and oil after enzymatic hydrolysis is difficult to break. Milk separation and other technical problems

Method used

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  • Method for extracting soybean oil and proteins

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Effect test

Embodiment 1

[0018] After the soybeans are cleaned and crushed, they are mixed with water to form a mixed solution, and 2% alkaline protease is added to the mixed solution at an ultrasonic power of 350W, a liquid-solid ratio of 7:1mL / g, a reaction temperature of 55°C, and a reaction pH of 8.5 Ultrasonic assisted enzymolysis for 1.5h, and then centrifuged to obtain soybean oil, emulsion, hydrolyzate and residue; under the condition of ionic strength of 0.1mol / L KCl, the surfactant SDS with a concentration of 0.08g / mL and the volume ratio Mix 4:1 n-hexane / n-octanol to form SDS / n-hexane / n-octanol reverse micelle system, and add to the emulsion and hydrolyzate at an extraction pressure of 14Mpa, an extraction temperature of 50°C, and an extraction pH of 8 Add SDS / n-hexane / n-octanol reverse micelles system for high-pressure reverse micellar extraction for 10 minutes to obtain extract, mix the extract with equal volume of 1mol / L KCl solution for back extraction, and centrifuge after back extracti...

Embodiment 2

[0020] Clean and crush the soybeans and mix them with water to form a mixed solution. Add 2.5% alkaline protease to the mixed solution at an ultrasonic power of 300W, a liquid-to-solid ratio of 8:1mL / g, a reaction temperature of 60°C, and a reaction pH of 8. Ultrasound-assisted enzymolysis was carried out for 1 h, and then centrifuged to obtain soybean oil, emulsion, hydrolyzate and residue; under the condition of ionic strength of 0.1mol / L KCl, the volume ratio of the surfactant SDS with a concentration of 0.08g / mL was Mix 4:1 n-hexane / n-octanol to form SDS / n-hexane / n-octanol reverse micelle system, and add it to the emulsion and hydrolyzate at an extraction pressure of 12Mpa, an extraction temperature of 55°C, and an extraction pH of 9 The SDS / n-hexane / n-octanol reverse micellar system was subjected to high-pressure reverse micellar extraction for 15 minutes to obtain the extract, and the extract was mixed with 1mol / L KCl solution in equal volumes for back extraction, and aft...

Embodiment 3

[0022] After the soybeans are cleaned and crushed, they are mixed with water to form a mixed solution, and 1.5% alkaline protease is added to the mixed solution at an ultrasonic power of 400W, a liquid-to-material ratio of 6:1mL / g, a reaction temperature of 55°C, and a reaction pH of 7.5 Ultrasonic assisted enzymolysis for 1.5h, and then centrifuged to obtain soybean oil, emulsion, hydrolyzate and residue; under the condition of ionic strength of 0.1mol / L KCl, the surfactant SDS with a concentration of 0.08g / mL and the volume ratio Mix 4:1 n-hexane / n-octanol to form SDS / n-hexane / n-octanol reverse micelle system, and inject it into the emulsion and hydrolyzate at an extraction pressure of 16Mpa, an extraction temperature of 50°C, and an extraction pH of 7 Add SDS / n-hexane / n-octanol reverse micelles system for high-pressure reverse micellar extraction for 10 minutes to obtain extract, mix the extract with equal volume of 1mol / L KCl solution for back extraction, and centrifuge aft...

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Abstract

The invention discloses a method for extracting soybean oil and proteins and belongs to an extraction processing technology of plant oil and proteins. The method comprises the following steps: (1) cleaning and grinding soybeans, mixing the soybeans with water to form a mixed solution, adding protease into the mixed solution for performing ultrasonic-assisted enzymolysis treatment, and performing centrifugal separation to obtain soybean oil, emulsion, hydrolysate and residues; (2) adding a reverse micelle system into the emulsion and the hydrolysate for performing high-pressure reverse micelle extraction, performing back extraction on the extracting solution, and performing centrifugal separation to obtain a surfactant phase, an organic phase and an aqueous phase; (3) performing rotary evaporation on the organic phase to obtain the soybean oil, performing ultrafiltration on the aqueous phase, and performing vacuum drying and freeze drying to obtain the soybean protein. According to the method, the ultrasonic technology, the enzymolysis technology, the high-pressure technology and the reverse micelle extraction technology are organically combined together, the method is applied to extraction processing of the soybean oil and proteins, the extraction efficiency is effectively improved, the prepared soybean oil is high in quality, and high-purity low-degeneration soybean proteins can be obtained.

Description

technical field [0001] The invention belongs to the extraction and processing technology of vegetable oil and protein, and mainly relates to a method for extracting soybean oil and protein. Background technique [0002] Enzymatic hydrolysis technology is a new technology. Applying enzymatic hydrolysis technology to oil plants can extract oil and protein at the same time. Enzymolysis technology is favored by researchers and industrialists because of its mild reaction conditions and high extraction efficiency. This technology has mild treatment conditions and simple process route, but it is also a process method that is difficult to study, especially for oils with low oil content, the oil yield is low, and the emulsion formed by protein and oil after enzymatic hydrolysis is difficult to break. Milk separation and other technical problems. [0003] Ultrasonic technology is widely used in destroying the cell wall structure of oil plants. Ultrasonic wave is an elastic mechanica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14C11B1/10C11B1/04
Inventor 李杨江连洲隋晓楠齐宝坤王中江冯红霞王欢丁俭马文君王瑞赵城彬
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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