Process for extracting rapeseed protein

A rapeseed protein and process technology, applied in the field of extracting rapeseed protein, can solve the problems of low protein extraction rate, etc., and achieve the effects of high protein extraction rate, low production cost, and reduced losses.

Inactive Publication Date: 2016-02-03
周广安
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the shortcomings of the low protein extraction rate of the traditional rapeseed protein extraction method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment l

[0019] (1) Preparation of rapeseed meal powder:

[0020] The high-quality double-low rapeseeds produced in 2008 were selected, hulled on a huller, and air-selected after treatment. The shelled rapeseed is crushed on a hammer cyclone mill, and passed through a 100-mesh sieve after crushing. Use petroleum ether (boiling range of 30-60°C) on a Soxhlet extractor to deoil the crushed rapeseed at 50°C, and place it in a ventilated place for precipitation after treatment, so that the petroleum ether can be completely volatilized. Ventilated and dried again, the obtained is the rapeseed meal powder required for the test, and its protein (N×6.25 dry basis) content was measured to be 50.1%.

[0021] (2) Configuration of reverse micellar solution

[0022] Preparation of KCl buffer solution: Weigh 110.8g disodium hydrogen phosphate dihydrate and 9.07g potassium dihydrogen phosphate to prepare 1L of solution, mix 190mL and 10mL respectively, and then add 0.596g potassium chloride to the ...

Embodiment 2

[0034] (1) Preparation of rapeseed meal powder:

[0035] The high-quality double-low rapeseeds produced in 2008 were selected, hulled on a huller, and air-selected after treatment. The shelled rapeseed is crushed on a hammer cyclone mill, and passed through an 80-mesh sieve after crushing. Use petroleum ether (boiling range of 30-60°C) on a Soxhlet extractor to deoil the crushed rapeseed at 50°C, and place it in a ventilated place for precipitation after treatment, so that the petroleum ether can be completely volatilized. Ventilated and dried again, the obtained rapeseed meal powder required for the test, its protein (N×6.25, dry basis) content was measured to be 45.6%.

[0036] (2) Preparation of reverse micellar solution

[0037] KCl buffer solution preparation: prepare 0.2mol.L -1Disodium hydrogen phosphate solution and 0.1mol.L -1 1L of each citric acid solution, take 10.30mL and 9.70mL respectively and mix well. Get 0.1193g potassium chloride again and add in the ab...

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PUM

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Abstract

The invention relates to a process for extracting rapeseed protein. The process is simple, and has the advantages that the degree of protein denaturation is greatly reduced, the protein extraction rate is high, and the loss of a surfactant is remarkable reduced; besides, water resources are saved, and energy consumption in industrial production is lowered. In the process for extracting the rapeseed protein by use of reverse micelle, a method for backward extraction comprises the following steps: extracting the rapeseed protein by use of a reverse micelle solution to obtain a forward extraction solution; conducting vacuum concentration on the forward extraction solution to remove an organic solvent in the reverse micelle solution; then, adding a buffer solution for extraction and separation, so as to obtain an aqueous solution of the rapeseed protein. A method for extracting the rapeseed protein by use of the reverse micelle solution comprises the following steps: adding powder of rapeseed dregs into the reverse micelle solution; carrying out extraction under the ultrasonic action; then, carrying out solid-liquid separation to obtain the forward extraction solution. According to the process, vacuum concentration is conducted through rotary evaporation, and the water bath temperature is controlled to be 50-70 DEG C; the process is simple, lower in resource consumption, low in production cost, mild in operating condition, and high in protein extraction rate.

Description

technical field [0001] The invention relates to a process for extracting rapeseed protein. Background technique [0002] Rapeseed is an oil crop produced in a large area in my country, and its output ranks first in the world. The amount of by-products produced during the processing of rapeseed oil is huge, and the output of rapeseed meal alone exceeds 10 million tons per year. Rapeseed meal contains 35% to 42% crude protein, and the content of lysine, cystine and methionine in rapeseed protein is relatively high, and the amino acid balance is better than that of soybean protein. It is a source of plant protein. [0003] At present, using rapeseed or rapeseed meal as raw material, the methods for producing rapeseed protein mainly include aqueous phase method, aqueous phase enzymatic hydrolysis method, organic solvent extraction, ultrafiltration, diafiltration and other methods. The aqueous phase method often has a low protein extraction rate in industrial production, and th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/30
Inventor 周广安
Owner 周广安
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