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Method for extracting sturgeon skin collagen

An extraction method, skin collagen technology, applied in the field of sturgeon skin collagen extraction, can solve problems such as difficult to achieve extraction effect, hydrolysis of collagen peptide bonds, loss of collagen, etc., to improve the economic benefits of production enterprises, prevent hydrolysis, improve The effect on product yield

Inactive Publication Date: 2009-07-08
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some of these methods will cause hydrolysis of collagen peptide bonds, destroy all amino acids containing hydroxyl and sulfhydryl groups, and denature collagen; some will cause a large amount of collagen loss
In particular, sturgeon skin is different from most fish cortex materials. The skin is thick and hard, and it is difficult to achieve the ideal extraction effect by ordinary extraction methods. Therefore, it is necessary to improve the extraction method of sturgeon skin collagen.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Take the sturgeon skin and cut into small pieces, and place in 0.025mol / L Ca(OH) 2 Soak in the solution, the weight of the raw material of the sturgeon skin and Ca(OH) 2 The volume ratio of the solution is 1:6. After soaking for 60 minutes, take it out for rinsing, and then soak it in 0.15 mol / L acetic acid solution for about 60 minutes, then take it out for rinsing and set aside;

[0018] (2) The pre-treated sturgeon skin is added to water and extracted at 50°C for about 18 hours, and the ratio of the weight of the sturgeon skin material to the volume of water is 1:3 to obtain the collagen extract;

[0019] (3) Sturgeon skin collagen powder is obtained by freezing and centrifuging. The product yield is 6.28%, the gel strength of the 50mg / mL collagen solution is 1218 g.cm, the viscosity is 2.28 Pa.s, the product is white in color and has good brightness.

Embodiment 2

[0021] (1) Cut the skin of the sturgeon into small pieces, and place in 0.02mol / L Ca(OH) 2 Soak in the solution, the weight of the raw material of the sturgeon skin and Ca(OH) 2 The volume ratio of the solution is 1:6. After soaking for 50 minutes, rinse, and then soak with 0.175mol / L sulfuric acid solution for about 50 minutes, then take it out and rinse for use;

[0022] (2) The pre-treated sturgeon skin is added to water and extracted at 45°C for about 16 hours, and the ratio of the weight of the sturgeon skin material to the volume of water is 1:3 to obtain the collagen extract;

[0023] (3) Sturgeon skin collagen powder is obtained by freezing and centrifuging. The product yield is 5.71%, the gel strength of the 50mg / mL collagen solution is 1886 g.cm, the viscosity is 1.804 Pa.s, the product is white in color and has good brightness.

Embodiment 3

[0025] (1) Take the sturgeon skin and cut it into small pieces, soak in 0.03mol / L NaOH solution. The weight ratio of the sturgeon skin raw material to the volume of NaOH solution is 1:6. After immersing for 70 minutes, rinse, and then use 0.2mol / L. Soak in L citric acid solution for about 70 minutes, then take it out and rinse for later use;

[0026] (2) The pre-treated sturgeon skin is added to water and extracted at 55°C for about 17 hours, and the ratio of the weight of the sturgeon skin material to the volume of water is 1:3 to obtain the collagen extract;

[0027] (3) Sturgeon skin collagen powder is obtained by freezing and centrifuging. The product yield is 5.29%, the gel strength of the 50mg / mL collagen solution is 1904 g.cm, the viscosity is 1.89 Pa.s, the product is white in color and has good brightness.

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Abstract

The invention discloses an extracting method for a sturgeon skin collagen. The extracting method comprises the following steps: (1) preliminary treatment: taking and slicing a sturgeon skin, firstly immersing the sturgeon skin in alkaline solution, immersing the sturgeon skin in an acidic solution after rinsing, and then taking the sturgeon skin out for rinsing and standby; (2) extraction: adding water into the preprocessed sturgeon skin for leaching at 45 to 55 DEG C, and carrying out the filtration to remove impurities and obtain the collagen extracting solution; and (3) freeze drying: obtaining sturgeon skin collagen powder through the refrigerated centrifugation of the collagen extracting solution. The collagen product has higher quality. The extracting method has a higher collagen extraction rate, and not only improves the product yield, but also saves the cost in a simple way, thereby improving the economic benefits of a manufacturing enterprise.

Description

Technical field [0001] The invention relates to a method for extracting collagen, in particular to a method for extracting collagen from sturgeon skin. Background technique [0002] With the success and promotion of sturgeon artificial propagation and breeding technology, my country's sturgeon breeding industry has achieved remarkable results, and the breeding area and production of sturgeon have been increasing year by year. As the core product of sturgeon processing, sturgeon roe accounts for about 10% of the total weight of mature female sturgeon. Most of the other parts are discarded as waste, which not only wastes resources but also pollutes the environment. Among them, sturgeon skin occupies a large proportion of the waste. Because it is rich in collagen, it is an excellent source for the development of collagen products. [0003] At present, collagen extraction methods at home and abroad mainly include alkaline method, hot water extraction, salt method, acid method, enzyma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C07K1/14
Inventor 郝淑贤李来好杨贤庆岑剑伟石红刁石强陈胜军黄卉戚勃吴燕燕周婉君马海霞
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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