11results about How to "Increased soluble protein content" patented technology

The invention belongs to the technical field of crop cultivation, and particularly relates to application of gamma-aminobutyric acid in relieving saline-alkali stress of cotton and a method for relieving the saline-alkali stress of the cotton. According to the method, gamma-aminobutyric acid is used as an exogenous additive, and when the cotton is stressed by a secondary salt solution with the salt content of 0.45%-0.47% and the pH value of 7.5-8.0, the gamma-aminobutyric acid is added into the secondary salt solution; gamma-aminobutyric acid relieves secondary salt solution stress by improving superoxide dismutase activity of cotton, improving peroxidase activity of cotton, reducing malondialdehyde content of cotton, improving catalase activity of cotton, reducing superoxide anion content, reducing H2O2 content of cotton and improving soluble protein content of cotton; compared with other plant growth regulators, the gamma-aminobutyric acid has higher cotton saline-alkali stress relieving activity. And the gamma-aminobutyric acid is an amino acid naturally existing in an organism, has no toxicity to a human body, has no residual toxicity and residual effect after being used for treating cotton, and has practical utilization value.

The invention provides research on an action mechanism of exogenous selenium and silicon on tomato growth and development, and relates to the technical field of agricultural research, cherry tomatoes are used as materials, Na2SeO3 is used as a selenium source, Na2S O3 is used as a silicon source, ultrapure water is used for dissolution, and influences of selenium (Se) and silicon (S ) with different concentrations on tomato growth characteristics and fruit quality are researched. Exogenous addition of Se and Si fertilizers helps to promote increase of plant height, stem diameter and dry and fresh weight of tomato plants, increase chlorophyll content of seedling leaves and improve photosynthesis of the plants, additional application of Se, Si and Se + Si fertilizers plays a positive role in improvement of fruit quality, and compared with a contrast, the application of Se, Si and Se + Si fertilizers has the advantage that the application amount of Se, Si and Se + Si fertilizers is greatly reduced. Se, S and Se-S fertilizers are sprayed on leaf surfaces, so that the single fruit weight, the fruit hardness and the soluble protein content of tomato fruits can be improved, the content of soluble solids, soluble sugar, titratable acid, vitamin C, flavonoid and total phenols of the fruits can be increased, and the treatment effect is the best when Se with the concentration of 4 mg / L and S with the concentration of 1.5 mmol / L are adopted.

The invention discloses an application of an overexpressed CrOASTL1 gene in improving the soluble protein content of leguminous forage, and belongs to the technical field of biology. 1, and the CDS sequence of the CrOASTL1 gene is as shown in SEQ ID NO. 2. The method comprises the following steps: cloning a CrOASTL1 gene segment, constructing a plant overexpression vector, carrying out plant transgenosis operation, and introducing into leguminous forage plant cells to obtain a leguminous forage transgenosis line with the overexpressed CrOASTL1 gene. The CrOASTL1 gene is over-expressed in medicago sativa, and the soluble protein content of leaves of the medicago sativa is remarkably increased by about 19%. The CrOASTL1 gene of chlamydomonas reinhardtii is applied to quality improvement of leguminous forage for the first time, the technical obstacle that the genetic relationship of species is relatively far is overcome, a brand new gene resource and an efficient technical approach are provided for cultivating a new variety of leguminous forage with high protein and high nutritional value, and the method has important significance in promoting sustainable development of grass and animal husbandry.

The invention belongs to the technical field of egg product processing, and particularly relates to a preparation method of low-viscosity high-temperature-resistant egg white liquid. According to the preparation method of the high-temperature-resistant egg white liquid, an interaction mode of egg white components is regulated and controlled through ultrasonic / pH cooperative treatment, so that the heat stability of fresh egg white under the heating condition is improved, and then the high-temperature-resistant egg white liquid is obtained. The method comprises the following steps: carrying out shell-egg separation on an egg to obtain a fresh egg white liquid; treating the fresh egg white liquid by using an ultrasonic pulverizer; adjusting the pH value to 10.6; packaging to obtain a finished product. The finally prepared low-viscosity high-temperature-resistant egg white liquid is still kept in a flowing state after being heated for 5 minutes at 72 DEG C, the turbidity is reduced from 1.87 to 0.38, the protein concentration is increased from 28.9 mg / mL to 96.74 mg / mL, and the gel point is increased from 63 DEG C to 76.5 DEG C. According to the preparation method of the low-viscosity high-temperature-resistant egg white liquid, a theoretical basis is provided for improving the processing applicability of egg white raw materials in downstream industries, and a technical support is provided for development of low-viscosity high-temperature-resistant egg white ingredients.

The invention relates to a preparation method and application of panchromatic luminous carbon dots sourced from tobacco waste. The preparation method comprises the following steps: step 1, drying tobacco waste, and crushing to obtain tobacco waste chippings; step 2, uniformly mixing one of water, absolute ethyl alcohol and acetone with the tobacco waste chippings and H2O2, standing for 5-30 minutes, and performing ultrasonic treatment by utilizing ultrasonic waves; step 3, filtering out tobacco waste debris residues to obtain a carbon dot crude solution containing blue carbon dots, green carbon dots and red carbon dots; 4, further filtering the carbon dot crude solution through a 0.22 mu m filter membrane, and dialyzing in ultrapure water to obtain a carbon dot concentrated solution; and 5, carrying out rotary evaporation on the carbon dot crude solution in a rotary evaporator at the temperature of 30-50 DEG C until the carbon dot crude solution is dried to obtain a carbon dot solid. By utilizing the tobacco waste, the problems of environmental pollution and resource waste caused by improper treatment of the tobacco waste are solved; compared with the existing method, the method is simpler in process, reduces the utilization of harmful chemical substances, and is suitable for large-scale production.

The invention discloses an application of Bacillus pumilus AD14 in improving saline-alkali soil and improving the resistance of soybean to saline-alkali stress, which belongs to the technical field of agricultural microorganisms. In order to screen Bacillus pumilus that can improve saline-alkali soil and significantly improve the resistance of soybean to saline-alkali stress, the invention screens a strain AD14 with good salt-alkali resistance from saline-alkali soil. The strain is identified as Bacillus pumilus ( Bacillus pumilus ), the experiment found that the strain has the growth-promoting properties of dissolving phosphorus, fixing nitrogen and producing pectinase; it can change the physical and chemical properties of saline-alkali soil, increase the activities of urease, sucrase and catalase in saline-alkali soil; improve the stress resistance of soybean in saline-alkali soil and promote the growth of soybean in saline-alkali soil. Therefore, Bacillus pumilus AD14 has broad application prospects in improving saline-alkali soil, improving the resistance of soybean to saline-alkali stress and promoting the growth and development of soybean under saline-alkali stress.

The invention discloses low-temperature-resistant burkholderia capable of dissolving phosphorus and potassium and application of the low-temperature-resistant burkholderia capable of increasing nutrition of green vegetables, the low-temperature-resistant burkholderia capable of increasing nutrition of the green vegetables is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.27769. The low-temperature-resistant burkholderia sp. Capable of dissolving phosphorus and potassium is obtained by carrying out low-temperature stress screening and ultraviolet mutagenesis on phosphorus-dissolving and potassium-dissolving bacteria, the ultraviolet mutagenesis power is 15W, the irradiation distance is 30cm, and the irradiation time is 30s. The low-temperature-resistant burkholderia capable of dissolving phosphorus and potassium has the effects of enriching soil nutrition and increasing nutrition of green vegetables.

The invention discloses a sisal hemp neo-pineapple mealybug breeding method which comprises the following steps: (1) selecting hydroponic pineapples as host plants, putting the hydroponic pineapples into a culture room, and transferring female adult sisal hemp neo-pineapple mealybug to be born onto the hydroponic pineapples; (2) after the female adult sisal hemp new pineapple mealybug grey insects produce nymphs, new hydroponic pineapples are placed every 10-15 days, the new hydroponic pineapples are placed at the positions partially overlapped with the leaves of the raw hydroponic pineapples, the raw hydroponic pineapples are taken out after the new hydroponic pineapples are placed for 1-2 days, the step of replacing the hydroponic pineapples is repeated, and the new hydroponic pineapples are taken out after the new hydroponic pineapples are placed in the positions partially overlapped with the leaves of the raw hydroponic pineapples. And carrying out subculture feeding on the sisal hemp and the neo-pineapple mealybug. By means of the method, the sisal hemp neo-pineapple mealybug can be bred in a laboratory, and the method has important significance on prevention and control research on the sisal hemp neo-pineapple mealybug.

The application relates to an application method of L-tert-leucine for improving the quality and storability of Gala apples, which comprises the following steps: selecting a test apple orchard base, selecting Gala apple trees, and spraying different concentrations of exogenous L-tert-leucine on the selected test apple trees in a fruit development period; picking 8-9 mature Gala fruits, storing the fruits, and comparing the fruit quality indexes of different groups and comparing various measured data. According to the application method, spraying 500 mg / L mass concentration L-tert-leucine on the leaves of Gala apples in a growth period can significantly promote the yellow coloration and brightness increase of the Gala apples at the time of harvesting, improve the fruit hardness, TSS content, TA content, soluble protein content and free amino acid content, is beneficial to maintaining the fruit hardness and soluble protein content during cold storage, delaying the decrease of the fruit hardness and soluble protein content, inhibiting the decrease of the TSS content and TA content and the increase of the free amino acid during cold storage, and keeping the Gala apple fruits with lower POD activity and higher SOD activity.

The invention belongs to a fermented milk technology, and particularly relates to a composite freeze-drying protective agent for probiotics, freeze-dried bacterial powder, fermented milk and application. The probiotics are streptococcus thermophilus JM905, and the composite freeze-drying protective agent is composed of 3-3.5 parts by weight of Arabic gum, 1-2 parts by weight of manganese sulfate, 2-3 parts by weight of maltodextrin and 2-4 parts by weight of L-sodium glutamate. The composite freeze-drying protective additive can significantly improve the freeze-drying survival rate of streptococcus thermophilus JM905, and overcomes the technical problem that probiotics are difficult to tolerate a freeze-drying process. The streptococcus thermophilus JM905 freeze-dried bacterial powder prepared from the streptococcus thermophilus JM905 freeze-dried bacterial powder can keep a relatively high viable count. Fermented milk prepared from the streptococcus thermophilus JM905 freeze-dried bacterial powder is high in free amino acid content, protein content and total nucleotide content, the digestibility is remarkably improved, and the soluble protein content after digestion of gastrointestinal tracts is improved.

The invention provides a drought-resistant growth-promoting plant endophytic bacillus strain, which is named as Bacillus sp.AB513 and preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M20242072, the preservation date is September 25, 2024, and the nucleotide sequence of 16S rRNA (Ribosomal Ribonucleic Acid) is as shown in SEQ ID NO.1. The drought-resistant growth-promoting plant endophytic bacillus strain has the advantages that the drought-resistant growth-promoting plant endophytic bacillus strain can be applied to drought-resistant growth-promoting plants; the invention further provides application of the strain. The strain is used for promoting plant growth under drought conditions. The strain provided by the invention can promote germination of alfalfa under drought conditions, increase root length and bud length of alfalfa seedlings, increase fresh weight and dry weight of the alfalfa seedlings under drought conditions, increase chlorophyll content of alfalfa cotyledons and increase soluble protein content of the alfalfa cotyledons.