Method for utilizing melatonin to reduce Pb accumulation amount of vegetables

A melatonin and accumulation technology, applied in botany equipment and methods, applications, gardening, etc., can solve the problems of reducing the accumulation of Pb in radish fleshy roots, and achieve safe and green production, low cost, simple and convenient production process Effect

Inactive Publication Date: 2018-01-16
NANJING AGRICULTURAL UNIVERSITY +1
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  • Abstract
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Problems solved by technology

There is no report that MT treatment reduces Pb accumulation in radish fleshy roots

Method used

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  • Method for utilizing melatonin to reduce Pb accumulation amount of vegetables
  • Method for utilizing melatonin to reduce Pb accumulation amount of vegetables
  • Method for utilizing melatonin to reduce Pb accumulation amount of vegetables

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Effects of Different Concentrations of Melatonin on Pb Content in Radish Plants

[0024] (1) Preparation of melatonin solution

[0025] The concentration of melatonin solution was 10, 25, 50, 100, 150 μmol / L respectively. The preparation method is as follows: dissolve 118.5mg of melatonin (Sigma) in 1.0mL of absolute ethanol, then dilute to 1L with distilled water to obtain a 500 μmol / L melatonin mother solution, and dilute other concentrations with water for preparation. All operations were carried out in the dark as far as possible, and the prepared solution was stored in a refrigerator at 4°C in the dark.

[0026] (2) Plant material

[0027] The radish seeds came from the high-generation inbred line 'NAU-YH' preserved in the radish laboratory of Nanjing Agricultural University. Radish seeds were soaked in 5mM sodium hypochlorite solution for 8-10min, rinsed with distilled water for 3 times, placed evenly in a petri dish covered with wet filter paper, and...

Embodiment 2

[0033] Example 2 Effects of Different Concentrations of Melatonin on the Antioxidant System of Radish Plants

[0034] Step (1), (2), (3) are the same as embodiment 1

[0035] (4) Determination of SOD, POD activity and soluble protein content

[0036] ① Weigh 0.2 g fresh samples of fleshy roots and leaves, wash them, put them in a pre-cooled mortar, add 1.6 mL (0.6 mL, 0.5 mL, 0.5 mL) of 50 mmol / L pre-cooled phosphate buffer solution in 3 times (pH 7.8), ground into a homogenate; ② Transfer to a 1.5mL centrifuge tube, centrifuge at 4°C, 12000rpm for 20min, the supernatant is the crude extract; ③ Determination of superoxide dismutase (SOD) activity: take 300μl Add 28 μl guaiacol and 19 μl 30% H 2 o 2 ) and 10 μl enzyme solution were added to the microplate; at the same time, two control tubes were made, each of which was added with 3 mL of reaction mixture and 100 μl of PBS, one of which was used as the maximum photoreduction tube, and the other was wrapped in tinfoil for sha...

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Abstract

The invention discloses a method for utilizing melatonin to reduce Pb accumulation amount of vegetables. The method for reducing the Pb accumulation amount of the vegetables comprises the step of spraying a melatonin solution to vegetable crop leaves. According to the method, by spraying the melatonin solution to the radish leaves, the activity of SOD and POD in plants under the threat of Pb can be effectively improved, the increase of the content of soluble protein is facilitated, and therefore the Pb accumulation amount in the fleshy roots and leaves of radishes is obviously reduced. The method has the advantages that the preparation process is simple and convenient, no pollution or drug damage is caused, the problem can be effectively solved that excessive Pb in the fleshy roots of theradishes is accumulated, the green and safe production of radishes is facilitated, and the method has broad application prospects.

Description

technical field [0001] The invention belongs to the field of crop cultivation, and relates to a method for reducing Pb accumulation in vegetables by using melatonin. Background technique [0002] In recent years, soil heavy metal pollution in my country has become increasingly serious. Lead (Pb), as a non-essential element for organisms, is one of the heavy metals with strong biological toxicity. Pb 2+ After being absorbed by plants, it accumulates in roots and shoots, which not only interferes with physiological and biochemical processes such as plant water absorption, ion transport, and cell metabolism, but also induces the production of active oxygen free radicals, resulting in oxidative damage to cells, seriously affecting the yield and quality of crops . Excess Pb 2+ It can be ingested by the human body through the food chain, and then participate in the combination of a series of functional groups such as amino acids, enzymes and proteins in the human body, causing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G22/25A01G13/00
Inventor 柳李旺徐良唐明佳王燕李翠
Owner NANJING AGRICULTURAL UNIVERSITY
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