Strain of saccharomycopsis fibuligera and application thereof

A technology for decapsulating and laminating, yeast, applied in the application, biochemical equipment and methods, organic fertilizers and other directions, can solve the problem of α-amylase activity, protease activity is not disclosed to produce other enzymes and the size of enzyme activity, and the variety of enzymes produced is rich. and high enzyme activity, acid resistance of undisclosed strains, etc., to achieve the effect of improving the quality of fruits and vegetables, high enzyme activity, and vigorous growth.

Active Publication Date: 2018-07-06
陕西枫丹百丽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] Chinese patent CN200710064203 discloses a fertilizer starter and its preparation method and application. The raw material formula of the fertilizer starter includes: 10-80 parts of fermentation industrial waste residue, 5-30 parts of straw, 5-30 parts of cake, bran and 10-30 parts of rice bran, 1.5-2 parts of urea, 0.5-1 part of NaCl and/or glucose, and Azotobacter chroococcum, Bacillus megaterium, Bacillus licheniformis, Lactobacillus plantarum ( Lactobacillus plantarum) and 1-5 strains in Saccharomycopsis fibuligera) and Saccharomycopsis fibuligera. The function of Saccharomycopsis fibuligera disclosed in this patent is to quickly remove soil compaction, and Utilize the characteristics of

Method used

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  • Strain of saccharomycopsis fibuligera and application thereof
  • Strain of saccharomycopsis fibuligera and application thereof
  • Strain of saccharomycopsis fibuligera and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0070] Example 1 Separation, purification, screening and identification of yeast Y-1

[0071] 1) Separation and purification: accurately weigh 10g of the distiller's grain sample taken from the natural accumulation and fermentation of Wuliangye Group in Yibin City, Sichuan Province, and add it to a sterile triangular flask with glass beads containing 100mL of cooled sterile water, and shake it on a 200r / min shaker Mix for 30 minutes, let stand for 5 minutes, draw 1 mL of supernatant, and dilute sequentially to obtain 10 -1 -10 -5 Concentration, select 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 Use a pipette to draw 100μL of each gradient suspension, and spread it evenly on the YPD plate with streptomycin sulfate. The final concentration of Streptomyces sulfate in the medium is 30μg / mL. Bacterial water control, put the plate upside down in an incubator, culture at 30°C for 7 days, and repeat 3 sets of each treatment setting level. According to the shape, size, surface structure, edge st...

Example Embodiment

[0112] Example 2 Low pH Tolerance Test of Saccharomyces cerevisiae Y-1

[0113] (1) Method: Pick an appropriate amount of Sporangium sphaerocephala Y-1 cells into YPD liquid medium, culture with shaking at 30°C and 180rpm for 48h to obtain the seed solution, and then connect the seed solution to different inoculation ratios at 0.5%. pH value (1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5) sterilized YPD liquid medium in the Erlenmeyer flask, each pH value is three replicates, and the non-inoculation is Blank control, 30℃, 180r / min shaking culture for 48h, observe whether the bacterial liquid in the Erlenmeyer flask is turbid and turbidity at each pH, ​​and measure the bacterial activity of the turbid bacterial liquid;

[0114] (2) Test results: Except for the pH value of 1.0, 1.5, 2.0 and the CK group bacteria liquid is still clear, other YPD liquid media with different pH (2.5-6.5) are all turbid, indicating that the yeast Y-1 It can grow in the range of pH 2.5-6.5, and t...

Example Embodiment

[0115] Example 3 Solid State Fermentation of Spore Spore Y-1

[0116] (1) Strain activation: Use an inoculating loop to pick up an appropriate amount of Dimosporium frondosa Y-1 and streak it on a PDA plate medium, and cultivate at 30°C for 48 hours;

[0117] (2) Preparation of the first-level seed solution: use an inoculating loop to pick up an appropriate amount of activated Sporangium saccharomyces cerevisiae Y-1 and inoculate it in YPD liquid medium, shake culture at 30°C and 200 rpm for 24 hours to obtain the first-level seed solution;

[0118] (3) Preparation of secondary seed liquid: inoculate the primary seed liquid into YPD liquid medium again according to the inoculation amount of 1% (v / v), shake culture at 30°C and 180 rpm for 36 hours to obtain secondary seed liquid;

[0119] (4) Solid-state fermentation research:

[0120] ① Mixing: According to the formula of solid fermentation medium A, first use 500mL tap water to mix glucose, peptone, (NH 4 ) 2 SO 4 , NaH 2 PO 4 , MgSO 4...

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Abstract

The invention belongs to the technical field of microbial fermentation, specifically belongs to the technical field of microbial decomposing, in particular relates to a strain which produces diversified enzymes with high enzyme activity and good acid resistance, enables distillers' grains to warm quickly and keep high temperature for a long time, and decomposes the distillers' grains completely, and relates to application of the strain. The strain is (Saccharomycopsis fibuligera)Y-1, and the preservation number is CGMCC No. 15255. The activity of an alpha-amylase produced by the strain is 2.56times higher than the currently known highest enzyme activity (7425.4 U/g); at the same time, the strain has the ability of producing glucoamylase, cellulase (CMC enzyme), cellulase (FPA enzyme), neutral protease, acid protease, lipase and other 7 enzymes, can produce diversified enzymes with high enzyme activity, and is applied to a microbial agent for fast decomposing to quickly and thoroughlydecompose the distillers' grains.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, in particular to the technical field of microbial decomposing, and in particular relates to a strain of spores of the distiller's grains, which can produce many kinds of enzymes, have high enzyme-producing activity, good acid resistance, fast heating of distiller's grains, long-lasting high temperature, and thorough decomposing of distiller's grains. Yeast, and relates to the application of the strain. Background technique [0002] Distiller's grains are the residual solids after cooking, saccharification and fermentation, distillation and other processes with sorghum, wheat, corn, rice, rye, etc. High acidity, high acidity, if it is allowed to discharge and is not treated in time, it will deteriorate and seriously pollute the environment. [0003] At present, many large-scale liquor companies choose to use distiller's grains for the second time, that is, to build steam workshops,...

Claims

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Application Information

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IPC IPC(8): C12N1/16C05G3/00C05G3/04C05F15/00C05F17/00C12R1/645C05G3/80
CPCC05F5/008C05F17/00C05G3/00C12N1/16C05G3/80C12N1/145C12R2001/645C05F11/02C05F11/08Y02W30/40
Inventor 李玲娣赵敬敬李爽王江王楠范君姣杜英侠袁志新刘桀
Owner 陕西枫丹百丽生物科技有限公司
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