The invention discloses a method for preparing 
ganoderma lucidum strains. The method comprises the following preparation steps of (1) preparation of a culture medium, wherein the culture medium comprises, by weight, 200 g of potatoes, 20 g of 
agar, 20 g of glucose, 2 g of peptone, 2 g of multivitamins, 2 g of 
potassium dihydrogen 
phosphate, 0.5 g of 
magnesium sulfate and 1000 ml of 
distilled water; (2) preparation of the strains, wherein preparation of the strains includes the following preparation steps that a, fresh 
ganoderma lucidum is selected for tissue separation; b, seeds are taken; c,
inoculation blocks are taken and inoculated into a 
test tube culture medium; d, the 
inoculation blocks are placed in a biochemical culture box for culture to obtain mother species; e, wood chips and the like are taken and placed in a 
bottle, and the mother species are inoculated into the 
bottle and grow for 45 days to obtain original species; f, the wood chips and the like are taken and placed inanother 
bottle, and the original species are inoculated into the bottle and grow for 45 days to obtain the strains. The method has the advantages that by adopting the method, the high-quality 
Ganoderma lucidum strains which are most suitable for large-scale planting can be obtained, the cultured 
Ganoderma lucidum strains are basically at the same strain age, and hyphae have high permeability and grow vigorously.