Method for preparing ganoderma lucidum strains

Abstract

The invention discloses a method for preparing ganoderma lucidum strains. The method comprises the following preparation steps of (1) preparation of a culture medium, wherein the culture medium comprises, by weight, 200 g of potatoes, 20 g of agar, 20 g of glucose, 2 g of peptone, 2 g of multivitamins, 2 g of potassium dihydrogen phosphate, 0.5 g of magnesium sulfate and 1000 ml of distilled water; (2) preparation of the strains, wherein preparation of the strains includes the following preparation steps that a, fresh ganoderma lucidum is selected for tissue separation; b, seeds are taken; c,inoculation blocks are taken and inoculated into a test tube culture medium; d, the inoculation blocks are placed in a biochemical culture box for culture to obtain mother species; e, wood chips and the like are taken and placed in a bottle, and the mother species are inoculated into the bottle and grow for 45 days to obtain original species; f, the wood chips and the like are taken and placed inanother bottle, and the original species are inoculated into the bottle and grow for 45 days to obtain the strains. The method has the advantages that by adopting the method, the high-quality Ganoderma lucidum strains which are most suitable for large-scale planting can be obtained, the cultured Ganoderma lucidum strains are basically at the same strain age, and hyphae have high permeability and grow vigorously.

Description

technical field [0001] The invention relates to the technical field of strain production, in particular to a preparation method of ganoderma lucidum strain. Background technique [0002] Ganoderma lucidum, commonly known as Ganoderma lucidum, is the fruiting body of the polyporaceae fungus Ganoderma lucidum. Ganoderma lucidum has the effects of invigorating qi and calming the nerves, relieving cough and relieving asthma. It is used for dizziness, insomnia, palpitation, shortness of breath, neurasthenia, exhaustion, cough and asthma. Ganoderma lucidum is a high-temperature fungus, which mainly grows in humid places. It needs fresh air and a high-humidity growth environment throughout its growth and development. At present, Ganoderma lucidum has been planted in artificial greenhouses. The fruiting bodies (or sclerotia) produced by traditional field collection or artificial cultivation are limited by the scarcity of wild resources, long fruiting body cultivation and production...

AI Technical Summary

Problems solved by technology

At present, Ganoderma lucidum has been planted in artificial greenhouses. The fruiting bodies (or sclerotia) produced by traditional field collection or artificial cultivation are limited by the scarcity of wild resources, long fruiting body cultivation and production cycle (4-5 months), and production is controlled by climate and other conditions. Restricted by many factors such as low content of bioactive substances and biologically active substances, the mass production of Ganoderma lucidum has been hindered, and the industrialization of Ganoderma lucidum has also been limited.