Method for quickly detecting whether or not schizophyllum commune liquid strain reaches standard and verification method of method

Abstract

The invention relates to a method for quickly detecting whether or not a schizophyllum commune liquid strain reaches the standard and a verification method of the method. The method for quickly detecting whether or not the schizophyllum commune liquid strain reaches the standard comprises the steps that firstly, a liquid culture medium is prepared, then a chizophyllum commune liquid strain seed solution with the mass accounting for 2-4% of the mass of the culture medium in a tank is put into the fermentation tank for culture, and a fermentation solution is obtained; 20-30 ml of the solution inthe tank is taken into a sterile bottle, shaking for bottle washing is conducted, the solution is poured out, 50 ml of the solution is taken for use again, a pH meter is used for calibration, then measurement is conducted, after the pH value keeps invariant for 5 minutes, reading is conducted, and when the pH value reaches 5.4+ / -0.1, the schizophyllum commune liquid strain reaches the use standard. The verification method comprises the steps that firstly, the dry weight of a hypha is measured, then the activity of the hypha and the diameter of the hypha ball are measured, and when the dry weight is maximum and the activity of the hypha ball is most vigorous, the end point pH value of the fermentation solution keeps within 5.4+ / -0.1. According to the method, through the specific culture medium and the strict liquid strain culture method, whether or not the liquid strain reaches the standard is conveniently monitored through the end point pH value.

Description

technical field [0001] The invention belongs to the technical field of liquid strain detection methods for edible fungi, and in particular relates to a detection method that can quickly detect whether liquid strains of schizophyllum have reached the use standard. Background technique [0002] Schizophyllum is a rare edible fungus, which is favored by many people, especially in Yunnan. It is also a folk health food for treating gynecological diseases and children's night sweats. Not only that, but the schizophyllan contained in it is an emerging preferred ingredient for skin care and health care, and it is also one of the material sources of anti-tumor drugs. However, due to the late start of cultivation, immature cultivation technology and small cultivation scale, the output is difficult to meet the market demand. In recent years, with the continuous innovation of cultivation technology, the factory cultivation of Schizophyllum has also achieved initial results. Based on th...

AI Technical Summary

Problems solved by technology

However, the process of detecting the amount of mycelium, mycelial activity, and mycelial ball size of liquid strains is cumbersome, time-consuming and troublesome, the detection efficiency is low, and the professional quality requirements for testing personnel are also high, which is not conducive to large-scale production.