41results about How to "Consistent bacterial age" patented technology

The invention provides a method of producing Agrocybe aegerita strains by liquid culture and a preservation method of the Agrocybe aegerita strains. The method includes the steps and process parameters: liquid culture efficient strains are screened, namely the liquid culture strains with many pellets, uniform size and many surface burrs are optimally selected, the amount of culture pellets is 200-260/ml, the pellets are 1.0-1.5mm in diameter, a shake-flask culture formula includes 1-3% of glucose, 2-4% of corn flour, 0.2-0.4% of yeast powder, 1.0-3.0% of wheat bran, 0.5-1.5% of KH2PO4 and 0.5-1.0% of MgSO4, with natural PH value, the culture medium formula for fermenting tank culture of Agrocybe aegerita liquid strains is provided, and process conditions for fermenting tank culture of Agrocybe aegerita liquid strains are established. The key technical problems of production of Agrocybe aegerita strains are solved by the production of Agrocybe aegerita strains by liquid culture; the production cycle is short, production cost is low, fruiting is even after inoculation, and management is facilitated.

The invention relates to the field of mushroom cultivation technologies, in particular to a method for manufacturing edible mushroom solid liquefied strains. The method comprises the steps that first-level liquid strains are inoculated into a culture flask, and liquid spawn is obtained; a solid cultivation material is prepared, the liquid spawn is cultured in strain bottles, the cultivation temperature ranges from 22 DEG C to 25 DEG C, cultivation is conducted in a dark place, the cultivation humidity ranges from 50% to 60%, and the strains can grow full of the strain bottles after being cultured by 20-30 days; the strain bottles filled with the strains and having no infectious microbe are selected, and the strains are crushed in a pulverizer and inoculated into sterile water for direct production and inoculation. The method for manufacturing the edible mushroom solid liquefied strains has the advantages that the strain running speed is high and is 1/3 higher than that of solid strain running, 3-4 times of expansion inoculation are not needed, the strain contamination problem caused in the expansion inoculation process is solved, and time needed by expanding propagation is saved; growing points are more than the growing points of common liquid strains, strain running is uniform, the strain ages are consistent, produced mushrooms are orderly and good in quality, and fruit body yield can be improved by 20%-30%; sterile water is adopted for liquefying mycelia, molds and other infectious microbes can not grow, and contamination can be reduced by 5%-10%.

The invention discloses a method for liquid fermentation cultivation of Agaricus bisporus strain, which includes: firstly, inoculating activated slant culture 0.5cm2 into a 250mL culture bottle containing 60-120mL of liquid medium, controlling temperature to be 21-27 DEG C, shaking at 90-180rpm, culturing for 5-7 days to obtain primary shaking strain, transferring primary seeds accounting for 5-10% of the inoculation amount to secondary culture liquid for cultivation, transferring cultured secondary shaking liquid strain to tertiary culture liquid for cultivation, and sequentially performing all levels of cultivation to obtain seed broth by the same methods; and secondly, inoculating the seed broth accounting for 5% of the inoculation amount into a 10L fermentation jar containing 6L of fermentation medium for cultivation, and culturing at 25 DEG C for 5-6 days. The method is characterized by short production cycle, uniform fungus age, low production cost and simplicity in inoculation, a test tube of strain can be multiplied by 200000 times by five levels of cultivation, the secondary liquid strain is evidently faster than traditional solid spawn in growth speed, and the speed is increased by 33%. In addition, the growth speeds of all levels of the liquid strain are nearly equal, the average fullness time is 27.5 days, and the method is absolutely applicable to practice of factory production.

The invention provides a selenium-rich culture medium for hericium erinaceus. The selenium-rich culture medium for hericium erinaceus is mainly prepared from wood flour, bran, selenium-rich fungus grass and gypsum. The invention further provides a method for producing hericium erinaceus with the selenium-rich culture medium for hericium erinaceus. A traditional production method of a hericium erinaceus strain is changed, a liquid strain technology is utilized in hericium erinaceus strain production, and a selenium-rich liquid strain is prepared; meanwhile, the solid selenium-rich culture medium and a secondary selenium enrichment method are adopted, and produced hericium erinaceus is high in selenium content and high in nutritive value. Production efficiency is improved, and production cost is greatly reduced; besides, the production method is simple in process, and the produced strain is high in utilization rate and low in contamination rate.

The invention provides a liquid fermentation culture medium for shiitake mushrooms. The liquid fermentation culture medium is composed of main raw materials including bran, bean pulp, glucose, monopotassium phosphate, magnesium sulfate and sodium carboxymethylcellulose. The invention further provides a method for producing the shiitake mushrooms through the liquid fermentation culture medium for the shiitake mushrooms with the liquid strain technology. By means of the liquid fermentation culture medium and the method, a traditional method for producing shiitake mushroom strains is changed, the liquid strain technology is adopted in shiitake mushroom strain production, the production efficiency is improved, and the production cost is greatly saved; in addition, the production method is simple in technology, the produced strains are high in use rate, and the pollution rate is low.

The invention provides a selenium-rich Mythic Fungus medium. The medium mainly comprises wood chips, wheat bran, selenium-rich fungal grass and gypsum. The invention also provides a method for producing Mythic Fungus by using the selenium-rich Mythic Fungus medium. Traditional production methods of Mythic Fungus strains are changed, liquid selenium-rich strains are prepared by using a liquid strain technology in the Mythic Fungus strain production process, and Mythic Fungus produced by adopting the solid selenium-rich medium through a secondary selenium enrichment technology has the advantages of high content of the selenium content, and high nutrition values. The production method has the advantages of production efficiency increase, great saving of the production cost, simple process, high utilization rate of produced strains, and low pollution rate.

The invention discloses a method for preparing culture strains of Agaricus bisporus (Lange) Sing by fermenting culture materials and belongs to the technical field of culture of edible mushrooms. According to the method, 1,500kg of dried wheat straw (or rice straw), 1,500kg of dried cow dung, 25kg of urea, 50kg of lime powder, 30kg of calcium superphosphate, 50kg of gypsum powder and 40kg of calcium carbonate are used in the formula of the culture materials for culture strains of Agaricus bisporus (Lange) Sing. The culture materials are treated with outdoor fermentation and indoor post-fermentation technologies, a plastic basket which is 50cm long, 30 cm wide and 30 cm high is used as a container, and the culture strains of Agaricus bisporus (Lange) Sing are prepared through inoculation and cultivation. Compared with a currently commonly used method for preparing the culture strains from wheat serving as a main culture medium, the method has the advantages of grain saving, labor saving, energy saving, cost saving, shortening of strain preparation time, consistent culture age, short spawn running time after sowing and low probability of pollution of mixed fungi. The wheat straw (or rice straw) can be sufficiently utilized, environmental pollution caused by straw burning is reduced, and ecological balance is maintained.

The invention provides a culture medium of a mushroom liquid strain. The culture medium comprises the following main materials: wood chips, bacterial grass, bran, gypsum powder, monopotassium phosphate, magnesium sulfate and water. The invention also provides a method of producing a mushroom bacterium stick by utilizing the mushroom liquid strain. The culture medium provided by the invention has the advantages that the a conventional production method of the mushroom strain is changed, and by the utilization of a liquid-strain technology in the production of the mushroom bacterium stick, not only is the production efficiency improved, but also the production cost is greatly saved; the production method is simple in process, and the utilization rate of the produced strain is high and the pollution rate is low.

The invention provides a black fungus selenium-rich culture medium which consists of main raw materials including saw dust, bran, selenium-rich fungus grass and gypsum and further provides a method for producing black fungi by utilizing the black fungus selenium-rich culture medium. A traditional black fungus strain production method is changed, a liquid strain technology is utilized in black fungus strain production to prepare liquid selenium-rich strains, meanwhile the solid selenium-rich culture medium and a secondary selenium-rich method are adopted to make produced black fungi high in selenium content and high in nutritive value. The production efficiency is improved, the production costs are also greatly saved, the production method is simple in process, and the produced stains are high in utilization rate and low in pollution rate.

The invention provides a method for producing an edible fungi stock and a cultivated species by fungus rod fungi culture, which is high-efficiency large-scale edible fungus production technology capable of reducing the period of culture bag fungus culture. After a culture medium is filled into a culture bag by a bag filling machine or hands at a proper looseness, a more than 10 centimeter cone-shape fungus rod is inserted into the culture medium from the upper surface of the culture medium; the head of the fungus rod is flush with a culture material surface; and the head of the rod is fastened with a rope fastener. For inoculation in fungus bags, the fungus rod is pulled out, a fungus species is directly inoculated into the centers of the fungus bags under the condition of an inoculation amount no higher than that of surface solid inoculation, and the fungi grow toward four sides from the centers of the fungus bags. The spawn running time is reduced greatly, the survival rate is high, and both the spawn running and fungus age in the fungus bags are consistent. In a black fungus bag at a proper temperature, hyphae grow in the whole fungus bag after 25 to 30 days. The fungus rod fungi culture is a 'solidified liquid species' fungus culture technology advantageous over liquid species, requires a smaller fungus culture investment and can obviously improve economic benefits for cultivators.

The invention discloses a branch strain cultivation method of dictyophora rubrovalvata. The cultivation method includes the following steps: (1) selecting raw materials; (2) selecting branches; (3) processing the branches; (4) preparing corn syrup; (5) performing primary boiling; (6) performing secondary boiling; (7) mixing raw materials; (8) performing sterilization; and (9) performing inoculation. Through the utilization of poplar branches as carriers of strains, scattered germination can be realized when the carriers are placed in culture mediums, so that the strains can grow at 360 degrees; the inoculation method is fast in inoculation, germination and feeding, good in uniform stability of germination, consistent in strain age, fast in inoculation speed and suitable for large-scale production, and can effectively shorten germination time and guarantee inoculation vitality; and the branch strains cultivated by the cultivation method can have the stability of solid strains and the inoculation speed of liquid strain.

The invention belongs to the field of biology and particularly relates to a preparation method and application of a fungal suspension. The preparation method includes the steps of 1), preparing a liquid strain; 2), filtering and eluting; 3) adding water and grinding so as to obtain the fungal suspension. The fungal suspension has the advantages of strong mycelia vigor, many germination points, fungus age consistency, short production cycle, small pollution risks, low production cost and good economic benefit. The preparation method has the advantages that the defects of traditional solid strains, liquid strains and existing solid liquefied strains are overcome. The fungal suspension can be used for preparing liquid strains except for production and has a promising market prospect and huge economic value.

The invention discloses a method for preparing ganoderma lucidum strains. The method comprises the following preparation steps of (1) preparation of a culture medium, wherein the culture medium comprises, by weight, 200 g of potatoes, 20 g of agar, 20 g of glucose, 2 g of peptone, 2 g of multivitamins, 2 g of potassium dihydrogen phosphate, 0.5 g of magnesium sulfate and 1000 ml of distilled water; (2) preparation of the strains, wherein preparation of the strains includes the following preparation steps that a, fresh ganoderma lucidum is selected for tissue separation; b, seeds are taken; c,inoculation blocks are taken and inoculated into a test tube culture medium; d, the inoculation blocks are placed in a biochemical culture box for culture to obtain mother species; e, wood chips and the like are taken and placed in a bottle, and the mother species are inoculated into the bottle and grow for 45 days to obtain original species; f, the wood chips and the like are taken and placed inanother bottle, and the original species are inoculated into the bottle and grow for 45 days to obtain the strains. The method has the advantages that by adopting the method, the high-quality Ganoderma lucidum strains which are most suitable for large-scale planting can be obtained, the cultured Ganoderma lucidum strains are basically at the same strain age, and hyphae have high permeability and grow vigorously.

The invention belongs to the field of microorganisms, and discloses a Chinese saprophytic boletus liquid strain culture medium and a culture method. The culture medium is prepared from 150 to 200 g of potatoes, 10 to 25 g of bran, 10 to 15 g of glucose, 2 to 3 g of yeast powder, 1 to 2 g of CaCO3, 2 to 3 g of KH2PO4, 1 to 2 g of MgSO4, 1 to 2 tablets of vitamin B1 and 1000 mL of water. The culture medium provided by the invention is suitable for rapid growth of the Chinese saprophytic boletus, the rotating speed of a shaking table is controlled in different periods according to the growth characteristics of mycelia in the culture process, the recovery, germination and growth of the mycelia are promoted, the method is simple to operate and low in cost, mycelium pellets are fine and uniform, after mushroom sticks are inoculated, the mycelium pellets germinate in a multi-point mode, and therefore, the contact area between the mycelia and the culture material is effectively increased; compared with a solid strain, the mycelia are high in growth speed, low in pollution rate and consistent in strain age, and the strain culture period is effectively shortened.

The invention provides a method for preserving edible fungus strains by utilizing Mongolian oak fruits. The method comprises the following steps that a Mongolian oak fruit preservation culture medium is prepared, inoculating and bacterium culturing are carried out, and the strains are preserved. A formula of a strain preservation culture medium used in the method is prepared from 75% of Mongolian oak fruits, 24% of auxiliary materials (90% of broadleaf sawdust and 10% of wheat bran; and the water accounts for 60% of the mass of the dry material), 0.5% of gypsum and 0.5% of lime. The specific preservation method comprises the following steps that a to-be-preserved liquid strain is inoculated into the Mongolian oak preservation culture medium, a breathable film on a bottle cap is sealed by using a transparent polyethylene plastic film to isolate oxygen after hyphae grow all over the culture medium, then the culture medium is preserved in a refrigerator at 4 DEG C, and if the preservation time does not exceed 1 year, the culture medium can be stored at normal temperature. The method for preserving the edible fungus strains by utilizing the Mongolian oak fruits has the advantages of being long in strain preservation time, consistent in strain age, large in strain preservation amount and the like, and the strain preserved through the method is stable in hypha character, vigorous in growth vigor and high in vitality.

The invention relates to a method for quickly detecting whether or not a schizophyllum commune liquid strain reaches the standard and a verification method of the method. The method for quickly detecting whether or not the schizophyllum commune liquid strain reaches the standard comprises the steps that firstly, a liquid culture medium is prepared, then a chizophyllum commune liquid strain seed solution with the mass accounting for 2-4% of the mass of the culture medium in a tank is put into the fermentation tank for culture, and a fermentation solution is obtained; 20-30 ml of the solution inthe tank is taken into a sterile bottle, shaking for bottle washing is conducted, the solution is poured out, 50 ml of the solution is taken for use again, a pH meter is used for calibration, then measurement is conducted, after the pH value keeps invariant for 5 minutes, reading is conducted, and when the pH value reaches 5.4+/-0.1, the schizophyllum commune liquid strain reaches the use standard. The verification method comprises the steps that firstly, the dry weight of a hypha is measured, then the activity of the hypha and the diameter of the hypha ball are measured, and when the dry weight is maximum and the activity of the hypha ball is most vigorous, the end point pH value of the fermentation solution keeps within 5.4+/-0.1. According to the method, through the specific culture medium and the strict liquid strain culture method, whether or not the liquid strain reaches the standard is conveniently monitored through the end point pH value.