41results about How to "Consistent bacterial age" patented technology

The invention provides a method of producing Agrocybe aegerita strains by liquid culture and a preservation method of the Agrocybe aegerita strains. The method includes the steps and process parameters: liquid culture efficient strains are screened, namely the liquid culture strains with many pellets, uniform size and many surface burrs are optimally selected, the amount of culture pellets is 200-260 / ml, the pellets are 1.0-1.5mm in diameter, a shake-flask culture formula includes 1-3% of glucose, 2-4% of corn flour, 0.2-0.4% of yeast powder, 1.0-3.0% of wheat bran, 0.5-1.5% of KH2PO4 and 0.5-1.0% of MgSO4, with natural PH value, the culture medium formula for fermenting tank culture of Agrocybe aegerita liquid strains is provided, and process conditions for fermenting tank culture of Agrocybe aegerita liquid strains are established. The key technical problems of production of Agrocybe aegerita strains are solved by the production of Agrocybe aegerita strains by liquid culture; the production cycle is short, production cost is low, fruiting is even after inoculation, and management is facilitated.

The invention relates to the field of mushroom cultivation technologies, in particular to a method for manufacturing edible mushroom solid liquefied strains. The method comprises the steps that first-level liquid strains are inoculated into a culture flask, and liquid spawn is obtained; a solid cultivation material is prepared, the liquid spawn is cultured in strain bottles, the cultivation temperature ranges from 22 DEG C to 25 DEG C, cultivation is conducted in a dark place, the cultivation humidity ranges from 50% to 60%, and the strains can grow full of the strain bottles after being cultured by 20-30 days; the strain bottles filled with the strains and having no infectious microbe are selected, and the strains are crushed in a pulverizer and inoculated into sterile water for direct production and inoculation. The method for manufacturing the edible mushroom solid liquefied strains has the advantages that the strain running speed is high and is 1 / 3 higher than that of solid strain running, 3-4 times of expansion inoculation are not needed, the strain contamination problem caused in the expansion inoculation process is solved, and time needed by expanding propagation is saved; growing points are more than the growing points of common liquid strains, strain running is uniform, the strain ages are consistent, produced mushrooms are orderly and good in quality, and fruit body yield can be improved by 20%-30%; sterile water is adopted for liquefying mycelia, molds and other infectious microbes can not grow, and contamination can be reduced by 5%-10%.

The invention discloses a method for liquid fermentation cultivation of Agaricus bisporus strain, which includes: firstly, inoculating activated slant culture 0.5cm2 into a 250mL culture bottle containing 60-120mL of liquid medium, controlling temperature to be 21-27 DEG C, shaking at 90-180rpm, culturing for 5-7 days to obtain primary shaking strain, transferring primary seeds accounting for 5-10% of the inoculation amount to secondary culture liquid for cultivation, transferring cultured secondary shaking liquid strain to tertiary culture liquid for cultivation, and sequentially performing all levels of cultivation to obtain seed broth by the same methods; and secondly, inoculating the seed broth accounting for 5% of the inoculation amount into a 10L fermentation jar containing 6L of fermentation medium for cultivation, and culturing at 25 DEG C for 5-6 days. The method is characterized by short production cycle, uniform fungus age, low production cost and simplicity in inoculation, a test tube of strain can be multiplied by 200000 times by five levels of cultivation, the secondary liquid strain is evidently faster than traditional solid spawn in growth speed, and the speed is increased by 33%. In addition, the growth speeds of all levels of the liquid strain are nearly equal, the average fullness time is 27.5 days, and the method is absolutely applicable to practice of factory production.

The invention provides a selenium-rich culture medium for hericium erinaceus. The selenium-rich culture medium for hericium erinaceus is mainly prepared from wood flour, bran, selenium-rich fungus grass and gypsum. The invention further provides a method for producing hericium erinaceus with the selenium-rich culture medium for hericium erinaceus. A traditional production method of a hericium erinaceus strain is changed, a liquid strain technology is utilized in hericium erinaceus strain production, and a selenium-rich liquid strain is prepared; meanwhile, the solid selenium-rich culture medium and a secondary selenium enrichment method are adopted, and produced hericium erinaceus is high in selenium content and high in nutritive value. Production efficiency is improved, and production cost is greatly reduced; besides, the production method is simple in process, and the produced strain is high in utilization rate and low in contamination rate.

The invention provides a liquid fermentation culture medium for shiitake mushrooms. The liquid fermentation culture medium is composed of main raw materials including bran, bean pulp, glucose, monopotassium phosphate, magnesium sulfate and sodium carboxymethylcellulose. The invention further provides a method for producing the shiitake mushrooms through the liquid fermentation culture medium for the shiitake mushrooms with the liquid strain technology. By means of the liquid fermentation culture medium and the method, a traditional method for producing shiitake mushroom strains is changed, the liquid strain technology is adopted in shiitake mushroom strain production, the production efficiency is improved, and the production cost is greatly saved; in addition, the production method is simple in technology, the produced strains are high in use rate, and the pollution rate is low.

The invention provides a selenium-rich Mythic Fungus medium. The medium mainly comprises wood chips, wheat bran, selenium-rich fungal grass and gypsum. The invention also provides a method for producing Mythic Fungus by using the selenium-rich Mythic Fungus medium. Traditional production methods of Mythic Fungus strains are changed, liquid selenium-rich strains are prepared by using a liquid strain technology in the Mythic Fungus strain production process, and Mythic Fungus produced by adopting the solid selenium-rich medium through a secondary selenium enrichment technology has the advantages of high content of the selenium content, and high nutrition values. The production method has the advantages of production efficiency increase, great saving of the production cost, simple process, high utilization rate of produced strains, and low pollution rate.

The invention discloses a method for preparing culture strains of Agaricus bisporus (Lange) Sing by fermenting culture materials and belongs to the technical field of culture of edible mushrooms. According to the method, 1,500kg of dried wheat straw (or rice straw), 1,500kg of dried cow dung, 25kg of urea, 50kg of lime powder, 30kg of calcium superphosphate, 50kg of gypsum powder and 40kg of calcium carbonate are used in the formula of the culture materials for culture strains of Agaricus bisporus (Lange) Sing. The culture materials are treated with outdoor fermentation and indoor post-fermentation technologies, a plastic basket which is 50cm long, 30 cm wide and 30 cm high is used as a container, and the culture strains of Agaricus bisporus (Lange) Sing are prepared through inoculation and cultivation. Compared with a currently commonly used method for preparing the culture strains from wheat serving as a main culture medium, the method has the advantages of grain saving, labor saving, energy saving, cost saving, shortening of strain preparation time, consistent culture age, short spawn running time after sowing and low probability of pollution of mixed fungi. The wheat straw (or rice straw) can be sufficiently utilized, environmental pollution caused by straw burning is reduced, and ecological balance is maintained.

The invention provides a culture medium of a mushroom liquid strain. The culture medium comprises the following main materials: wood chips, bacterial grass, bran, gypsum powder, monopotassium phosphate, magnesium sulfate and water. The invention also provides a method of producing a mushroom bacterium stick by utilizing the mushroom liquid strain. The culture medium provided by the invention has the advantages that the a conventional production method of the mushroom strain is changed, and by the utilization of a liquid-strain technology in the production of the mushroom bacterium stick, not only is the production efficiency improved, but also the production cost is greatly saved; the production method is simple in process, and the utilization rate of the produced strain is high and the pollution rate is low.

The invention discloses a method for liquid fermentation cultivation of Pleurotus cornucopiae strain, which includes: firstly, inoculating slant mother culture into a 250mL culture bottle containing 120mL of liquid medium, controlling temperature to be 21-27 DEG C, shaking at 90-180rpm, culturing for 5-7 days to obtain primary shaking strain, transferring primary seeds accounting for 5-10% of the inoculation amount to secondary culture liquid for cultivation, transferring cultured secondary shaking liquid strain to tertiary culture liquid for cultivation, and sequentially performing all levels of cultivation to obtain seed broth by the same methods; and secondly, inoculating the seed broth accounting for 5% of the inoculation amount into a 10L fermentation jar containing 6L of fermentation medium for fermentation cultivation, and culturing at 25 DEG C for 5-6 days. The method is characterized by short production cycle, uniform fungus age, low production cost and simplicity in inoculation, the liquid strain is evidently faster than traditional solid spawn in growth speed, the culture bottle can be full of mycelia in 16 days, the time is shortened evidently, the growth speed of the liquid strain by inoculation is increased by 75%, and the method is absolutely applicable to practice of factory production.

The invention discloses an agaricus bisporus strain breeding method applicable to a standardized plant. An agaricus bisporus strain is taken as a mother strain for breeding, a fruiting experiment is performed, stock seeds are prepared, triticale is selected as a raw material, the raw material is soaked in a solution with the pH value ranging from 11.0 to 13.0 and stirred to adjust the pH value to range from 7.0 to 9.0, half of a bag of the raw material is quantitively packaged with a sterile bag, a lantern ring and a breather plug are used for sealing, sterilization is performed at the high temperature of 120-130 DEG C under the pressure of 0.10-0.20 MPa for 2-3 h, sterile inoculation is performed after cooling, and the agaricus bisporus strain is cultured at the constant temperature. By means of the method, high-quality, pure and high-yield agaricus bisporus cultivars can be bred in batches, the breeding cycle is shortened greatly, the pollution probability is reduced, the general breeding time of the cultivars lasts 15 days to 18 days, the water content of bred strain grains is consistent, the biofilm growing phenomenon is avoided, the qualification rate can reach 96%, and the method has broad practicability in strain breeding fields.

The invention relates to the field of mushroom cultivation processes, in particular to a method for producing solid liquefied strains of oyster mushrooms. First-grade liquid strains are inoculated in culture bottles to obtain liquid strains through cultivation; a solid culture compost is prepared, the liquid strains are cultivated in strain bottles, the cultivation temperature ranges from 22 DEG C to 25 DEG C, light-shielding cultivation is performed, cultivation humidity ranges from 50% to 60%, and the strain bottles can be full of strains after cultivation for 20 to 30 days; the strain bottles full of strains and free of contaminating microbes are selected out, and the strains are smashed in a smashing machine and are inoculated in sterile water to directly produce strains. By means of the method, strain growing speed is high and can be 1 / 3 higher than the solid strain growing speed, inoculation expanding of three to four times is not needed, the contamination problems in the inoculation expanding process are decreased, and the time required by propagation expanding is saved; growing points are more than growing points of common liquid strains, strains are consistent in growing speed and are relatively consistent in age, fruiting mushrooms are relatively order and good in quality, and fruiting body yield can be improved by 20%-30%; the sterile water is adopted to liquefy hyphae, mould and other contaminating microbes do not grow, and pollution rate can be reduced by 5%-10%.

The invention aims to provide a method for industrialized production of breathable Lentinula edodes fungus bags. The method includes adopting breathable plastic bags as Lentinula edodes fungus bags for cultivation, activating and culturing strains of Lentinula edodes, culturing for preparing kernel cultispecies and culturing the Lentinula edodes fungus bags by inoculating the cultispecies into the breathable Lentinula edodes fungus bags, culturing the breathable Lentinula edodes fungus bags, coloring and culturing Lentinula edodes industrially in a rack manner. By adopting the breathable Lentinula edodes fungus bags and combining the production steps of the method, the breathable Lentinula edodes fungus bags can be quickly produced in batch. Meanwhile, cost is reduced, production quality of the Lentinula edodes fungus bags is guaranteed, bag production and culturing period is shortened, operation is simplified, mechanical production is easily realized, utilization rate of culturing places is increased, labor is saved, and industrialized and quick cultivation of Lentinula edodes is realized, and the method has remarkable economic benefits.

The invention provides a black fungus selenium-rich culture medium which consists of main raw materials including saw dust, bran, selenium-rich fungus grass and gypsum and further provides a method for producing black fungi by utilizing the black fungus selenium-rich culture medium. A traditional black fungus strain production method is changed, a liquid strain technology is utilized in black fungus strain production to prepare liquid selenium-rich strains, meanwhile the solid selenium-rich culture medium and a secondary selenium-rich method are adopted to make produced black fungi high in selenium content and high in nutritive value. The production efficiency is improved, the production costs are also greatly saved, the production method is simple in process, and the produced stains are high in utilization rate and low in pollution rate.

The invention belongs to the technical field of edible fungi cultivation, and particularly relates to a cultivation technology for volvariella volvacea. Excellent strains are mainly selected with bran-contained liquid culture media, high-pressure steam sterilization is carried out, the inoculation amount is 10%, the strains are fixed on a shake bed of 200 r / min and cultivated for 144 h at the temperature of 32 DEG C, liquid strains of the volvariella volvacea are fermented and cultivated, and the liquid strains after cultivation are inoculated into cultivation bags with 100% mushroom dregs andcultivated under the aseptic condition. According to the volvariella-volvacea liquid strain formula and the clinker cultivation technology, high-quality volvariella-volvacea liquid strains are cultivated, wheat bran with the low cost is added into the culture media for cultivating the strains, cultivation is carried out with waste strain dregs, waste is turned into wealth, efficient production isachieved, and the economic cost is greatly saved.

The invention provides a method for producing an edible fungi stock and a cultivated species by fungus rod fungi culture, which is high-efficiency large-scale edible fungus production technology capable of reducing the period of culture bag fungus culture. After a culture medium is filled into a culture bag by a bag filling machine or hands at a proper looseness, a more than 10 centimeter cone-shape fungus rod is inserted into the culture medium from the upper surface of the culture medium; the head of the fungus rod is flush with a culture material surface; and the head of the rod is fastened with a rope fastener. For inoculation in fungus bags, the fungus rod is pulled out, a fungus species is directly inoculated into the centers of the fungus bags under the condition of an inoculation amount no higher than that of surface solid inoculation, and the fungi grow toward four sides from the centers of the fungus bags. The spawn running time is reduced greatly, the survival rate is high, and both the spawn running and fungus age in the fungus bags are consistent. In a black fungus bag at a proper temperature, hyphae grow in the whole fungus bag after 25 to 30 days. The fungus rod fungi culture is a 'solidified liquid species' fungus culture technology advantageous over liquid species, requires a smaller fungus culture investment and can obviously improve economic benefits for cultivators.

The invention discloses a branch strain cultivation method of dictyophora rubrovalvata. The cultivation method includes the following steps: (1) selecting raw materials; (2) selecting branches; (3) processing the branches; (4) preparing corn syrup; (5) performing primary boiling; (6) performing secondary boiling; (7) mixing raw materials; (8) performing sterilization; and (9) performing inoculation. Through the utilization of poplar branches as carriers of strains, scattered germination can be realized when the carriers are placed in culture mediums, so that the strains can grow at 360 degrees; the inoculation method is fast in inoculation, germination and feeding, good in uniform stability of germination, consistent in strain age, fast in inoculation speed and suitable for large-scale production, and can effectively shorten germination time and guarantee inoculation vitality; and the branch strains cultivated by the cultivation method can have the stability of solid strains and the inoculation speed of liquid strain.

The invention relates to a method for producing shell strains by using a shell-shaped strain inoculator. According to the technical scheme, a formula of a culture medium of the shell strains is composed of a main ingredient and accessories, wherein on the basis of 10000 shell-shaped strain inoculators, the main ingredient is 30-50kg of sawdust; and the accessories are composed of: 7-8kg of wheat bran, 0.5-1.5kg of corn flour, 0.2-0.6kg of gypsum, 0-0.5kg of lime, 0.1-0.2kg of monopotassium phosphate, 0.05-0.1kg of magnesium sulfate, 0.4-0.5kg of peptone and 0.4-0.5kg of glucose. After the main ingredient and accessories are stirred, filled into bottles, sterilized and subjected to inoculated culture, the shell strains are obtained. Compared with a conventional solid strain, the method provided by the invention has the advantages that the operation is convenient, the strain age is consistent, the strain purity is high and the seed applying cost is reduced; the time for culturing hyphaeis shortened by 10-15 days; and when a culturing bag is produced, the inoculation survival rate is increased to 100%, the pollution rate is reduced by 5% and the yield is increased by 5-10%.

The invention discloses a pleurotus eryngii liquid strain culture medium and a preparation method thereof. The culture medium consists of white sugar, soya bean meal, MgSO4, KH2PO4, emulsified silicone oil and water.

The purpose of the present invention is to provide a kind of technology of factory-produced air-permeable bacterium bag of shiitake mushroom: adopt air-permeable plastic bag as the bacterium bag container of shiitake cultivation, through the activation cultivation of shiitake mushroom strain, the cultivation of preparing barley cultivar and inoculation of cultivar In the step of cultivating mushroom bags in air-permeable bags, the air-permeable mushroom bags of shiitake mushrooms are cultivated, and the mushrooms are cultivated in a shelf-type factory through color change; the present invention adopts air-permeable plastic bags combined with the special manufacturing steps of the present invention, which can be mass-produced rapidly The shiitake mushroom bag can reduce the production cost at the same time, ensure the production quality of the shiitake mushroom bag, shorten the bag making and cultivation cycle, easy to operate, easy to mechanized production, increase the utilization rate of the cultivation site, save labor, and realize the rapid factory cultivation of the shiitake mushroom. Has significant economic benefits.

The invention belongs to the field of biology and particularly relates to a preparation method and application of a fungal suspension. The preparation method includes the steps of 1), preparing a liquid strain; 2), filtering and eluting; 3) adding water and grinding so as to obtain the fungal suspension. The fungal suspension has the advantages of strong mycelia vigor, many germination points, fungus age consistency, short production cycle, small pollution risks, low production cost and good economic benefit. The preparation method has the advantages that the defects of traditional solid strains, liquid strains and existing solid liquefied strains are overcome. The fungal suspension can be used for preparing liquid strains except for production and has a promising market prospect and huge economic value.

The invention discloses an excellent Chinese saprophytic bolete strain which is stable in cultivation character and high in yield. The Chinese saprophytic bolete strain BU001 is preserved in the China General Microbiological Culture Collection Center on May 17, 2021, and the preservation number of the Chinese saprophytic bolete strain BU001 is CGMCC No. 21959. According to the Chinese saprophytic bolete strain BU001 provided by the invention, a fungal colony is in a round or irregular shape, and hyphae are thick, strong and dense and are yellowish white; when the strain BU001 is used for preparing a liquid strain, hypha pellets are light brown and uniform in size, and the liquid strain can be prepared by fermenting and culturing for 6-8 days; when the strain is used for preparing and cultivating strains, the strain age is consistent, hyphae are golden and dense, the hybrid resistance is high, the spawn running speed is high, fruiting is neat, the yield is high, the cultivation period is short, and large-scale cultivation and production of the Chinese saprophytic boletus are facilitated. Therefore, the Chinese saprophytic boletus strain BU001 has important application value in germplasm resource protection, development, utilization and scientific research of the Chinese saprophytic boletus.

The invention discloses a method for preparing ganoderma lucidum strains. The method comprises the following preparation steps of (1) preparation of a culture medium, wherein the culture medium comprises, by weight, 200 g of potatoes, 20 g of agar, 20 g of glucose, 2 g of peptone, 2 g of multivitamins, 2 g of potassium dihydrogen phosphate, 0.5 g of magnesium sulfate and 1000 ml of distilled water; (2) preparation of the strains, wherein preparation of the strains includes the following preparation steps that a, fresh ganoderma lucidum is selected for tissue separation; b, seeds are taken; c,inoculation blocks are taken and inoculated into a test tube culture medium; d, the inoculation blocks are placed in a biochemical culture box for culture to obtain mother species; e, wood chips and the like are taken and placed in a bottle, and the mother species are inoculated into the bottle and grow for 45 days to obtain original species; f, the wood chips and the like are taken and placed inanother bottle, and the original species are inoculated into the bottle and grow for 45 days to obtain the strains. The method has the advantages that by adopting the method, the high-quality Ganoderma lucidum strains which are most suitable for large-scale planting can be obtained, the cultured Ganoderma lucidum strains are basically at the same strain age, and hyphae have high permeability and grow vigorously.

The invention discloses a method for liquid fermentation cultivation of Agaricus bisporus strain, which includes: firstly, inoculating activated slant culture 0.5cm2 into a 250mL culture bottle containing 60-120mL of liquid medium, controlling temperature to be 21-27 DEG C, shaking at 90-180rpm, culturing for 5-7 days to obtain primary shaking strain, transferring primary seeds accounting for 5-10% of the inoculation amount to secondary culture liquid for cultivation, transferring cultured secondary shaking liquid strain to tertiary culture liquid for cultivation, and sequentially performing all levels of cultivation to obtain seed broth by the same methods; and secondly, inoculating the seed broth accounting for 5% of the inoculation amount into a 10L fermentation jar containing 6L of fermentation medium for cultivation, and culturing at 25 DEG C for 5-6 days. The method is characterized by short production cycle, uniform fungus age, low production cost and simplicity in inoculation, a test tube of strain can be multiplied by 200000 times by five levels of cultivation, the secondary liquid strain is evidently faster than traditional solid spawn in growth speed, and the speed is increased by 33%. In addition, the growth speeds of all levels of the liquid strain are nearly equal, the average fullness time is 27.5 days, and the method is absolutely applicable to practice of factory production.

The invention relates to the field of mushroom cultivation processes, in particular to a method for producing solid liquefied strains of oyster mushrooms. First-grade liquid strains are inoculated in culture bottles to obtain liquid strains through cultivation; a solid culture compost is prepared, the liquid strains are cultivated in strain bottles, the cultivation temperature ranges from 22 DEG C to 25 DEG C, light-shielding cultivation is performed, cultivation humidity ranges from 50% to 60%, and the strain bottles can be full of strains after cultivation for 20 to 30 days; the strain bottles full of strains and free of contaminating microbes are selected out, and the strains are smashed in a smashing machine and are inoculated in sterile water to directly produce strains. By means of the method, strain growing speed is high and can be 1 / 3 higher than the solid strain growing speed, inoculation expanding of three to four times is not needed, the contamination problems in the inoculation expanding process are decreased, and the time required by propagation expanding is saved; growing points are more than growing points of common liquid strains, strains are consistent in growing speed and are relatively consistent in age, fruiting mushrooms are relatively order and good in quality, and fruiting body yield can be improved by 20%-30%; the sterile water is adopted to liquefy hyphae, mould and other contaminating microbes do not grow, and pollution rate can be reduced by 5%-10%.

The invention belongs to the field of microorganisms, and discloses a Chinese saprophytic boletus liquid strain culture medium and a culture method. The culture medium is prepared from 150 to 200 g of potatoes, 10 to 25 g of bran, 10 to 15 g of glucose, 2 to 3 g of yeast powder, 1 to 2 g of CaCO3, 2 to 3 g of KH2PO4, 1 to 2 g of MgSO4, 1 to 2 tablets of vitamin B1 and 1000 mL of water. The culture medium provided by the invention is suitable for rapid growth of the Chinese saprophytic boletus, the rotating speed of a shaking table is controlled in different periods according to the growth characteristics of mycelia in the culture process, the recovery, germination and growth of the mycelia are promoted, the method is simple to operate and low in cost, mycelium pellets are fine and uniform, after mushroom sticks are inoculated, the mycelium pellets germinate in a multi-point mode, and therefore, the contact area between the mycelia and the culture material is effectively increased; compared with a solid strain, the mycelia are high in growth speed, low in pollution rate and consistent in strain age, and the strain culture period is effectively shortened.

The invention relates to a method for producing shell strains by using a shell-shaped strain inoculator. According to the technical scheme, a formula of a culture medium of the shell strains is composed of a main ingredient and accessories, wherein on the basis of 10000 shell-shaped strain inoculators, the main ingredient is 30-50kg of sawdust; and the accessories are composed of: 7-8kg of wheat bran, 0.5-1.5kg of corn flour, 0.2-0.6kg of gypsum, 0-0.5kg of lime, 0.1-0.2kg of monopotassium phosphate, 0.05-0.1kg of magnesium sulfate, 0.4-0.5kg of peptone and 0.4-0.5kg of glucose. After the main ingredient and accessories are stirred, filled into bottles, sterilized and subjected to inoculated culture, the shell strains are obtained. Compared with a conventional solid strain, the method provided by the invention has the advantages that the operation is convenient, the strain age is consistent, the strain purity is high and the seed applying cost is reduced; the time for culturing hyphaeis shortened by 10-15 days; and when a culturing bag is produced, the inoculation survival rate is increased to 100%, the pollution rate is reduced by 5% and the yield is increased by 5-10%.

The invention provides a method for preserving edible fungus strains by utilizing Mongolian oak fruits. The method comprises the following steps that a Mongolian oak fruit preservation culture medium is prepared, inoculating and bacterium culturing are carried out, and the strains are preserved. A formula of a strain preservation culture medium used in the method is prepared from 75% of Mongolian oak fruits, 24% of auxiliary materials (90% of broadleaf sawdust and 10% of wheat bran; and the water accounts for 60% of the mass of the dry material), 0.5% of gypsum and 0.5% of lime. The specific preservation method comprises the following steps that a to-be-preserved liquid strain is inoculated into the Mongolian oak preservation culture medium, a breathable film on a bottle cap is sealed by using a transparent polyethylene plastic film to isolate oxygen after hyphae grow all over the culture medium, then the culture medium is preserved in a refrigerator at 4 DEG C, and if the preservation time does not exceed 1 year, the culture medium can be stored at normal temperature. The method for preserving the edible fungus strains by utilizing the Mongolian oak fruits has the advantages of being long in strain preservation time, consistent in strain age, large in strain preservation amount and the like, and the strain preserved through the method is stable in hypha character, vigorous in growth vigor and high in vitality.

The invention discloses a method for liquid fermentation cultivation of Pleurotus cornucopiae strain, which includes: firstly, inoculating slant mother culture into a 250mL culture bottle containing 120mL of liquid medium, controlling temperature to be 21-27 DEG C, shaking at 90-180rpm, culturing for 5-7 days to obtain primary shaking strain, transferring primary seeds accounting for 5-10% of the inoculation amount to secondary culture liquid for cultivation, transferring cultured secondary shaking liquid strain to tertiary culture liquid for cultivation, and sequentially performing all levels of cultivation to obtain seed broth by the same methods; and secondly, inoculating the seed broth accounting for 5% of the inoculation amount into a 10L fermentation jar containing 6L of fermentation medium for fermentation cultivation, and culturing at 25 DEG C for 5-6 days. The method is characterized by short production cycle, uniform fungus age, low production cost and simplicity in inoculation, the liquid strain is evidently faster than traditional solid spawn in growth speed, the culture bottle can be full of mycelia in 16 days, the time is shortened evidently, the growth speed of the liquid strain by inoculation is increased by 75%, and the method is absolutely applicable to practice of factory production.

The invention relates to a method for quickly detecting whether or not a schizophyllum commune liquid strain reaches the standard and a verification method of the method. The method for quickly detecting whether or not the schizophyllum commune liquid strain reaches the standard comprises the steps that firstly, a liquid culture medium is prepared, then a chizophyllum commune liquid strain seed solution with the mass accounting for 2-4% of the mass of the culture medium in a tank is put into the fermentation tank for culture, and a fermentation solution is obtained; 20-30 ml of the solution inthe tank is taken into a sterile bottle, shaking for bottle washing is conducted, the solution is poured out, 50 ml of the solution is taken for use again, a pH meter is used for calibration, then measurement is conducted, after the pH value keeps invariant for 5 minutes, reading is conducted, and when the pH value reaches 5.4+ / -0.1, the schizophyllum commune liquid strain reaches the use standard. The verification method comprises the steps that firstly, the dry weight of a hypha is measured, then the activity of the hypha and the diameter of the hypha ball are measured, and when the dry weight is maximum and the activity of the hypha ball is most vigorous, the end point pH value of the fermentation solution keeps within 5.4+ / -0.1. According to the method, through the specific culture medium and the strict liquid strain culture method, whether or not the liquid strain reaches the standard is conveniently monitored through the end point pH value.