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Liquid fermentation culture medium for shiitake mushrooms and method for producing shiitake mushrooms through the same

A technology of liquid fermentation and culture medium, applied in botany equipment and methods, fertilizer mixture, gardening, etc., can solve the problems of low efficiency, high cost, and long mycelium culture period of shiitake mushrooms, and achieve easy source, low price, and production short cycle effect

Active Publication Date: 2015-12-02
井冈山井祥菌草生态科技股份有限公司
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Problems solved by technology

[0007] In order to solve the problems of low efficiency, high cost and long mycelium cultivation period in the production method of shiitake mushrooms in the prior art, the present invention provides a production method of shiitake mushroom liquid strain with high production efficiency, short mycelium cultivation period and low cost

Method used

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  • Liquid fermentation culture medium for shiitake mushrooms and method for producing shiitake mushrooms through the same

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Embodiment 1

[0041] According to the formula of bran, weigh it according to a 500L standard fermentation tank: 10000g of bran, 5000g of soybean meal, 3000g of glucose, 500g of potassium dihydrogen phosphate, 250g of magnesium sulfate, 100g of CMC-Na (cellulase inducer), and put them into a 200L stainless steel frying pan. In a cooking pot, add 100L of water and stir to dissolve, boil for 20 minutes and then filter, add water to the filtrate to 500L, and add 350g of fresh soybean oil, set aside. The fermenter was emptied for 2 hours under the conditions of steam pressure 0.13-0.15Mpa and temperature 121°C; the above-mentioned prepared liquid fermenter culture medium was filled into tanks, and it was sterilized under the conditions of steam pressure 0.13-0.15Mpa and temperature 121°C for 40 minutes. After the internal temperature of the liquid in the fermenter is cooled to below 28° C., inoculate under sterile conditions with an inoculum size of 2% (v / v). Fermentation culture temperature is ...

Embodiment 2

[0043] According to the formula of bran, weigh it according to a 500L standard fermentation tank: 10000g of bran, 5000g of soybean meal, 3000g of glucose, 500g of potassium dihydrogen phosphate, 250g of magnesium sulfate, 100g of CMC-Na (cellulase inducer), and put them into a 200L stainless steel frying pan. In a cooking pot, add 100L of water and stir to dissolve, boil for 20 minutes and then filter, add water to the filtrate to 500L, and add 350g of fresh soybean oil, set aside. The fermenter was emptied for 2 hours under the conditions of steam pressure 0.13-0.15Mpa and temperature 121°C; the above-mentioned prepared liquid fermenter culture medium was filled into tanks, and it was sterilized under the conditions of steam pressure 0.13-0.15Mpa and temperature 121°C for 40 minutes. After the internal temperature of the liquid in the fermenter is cooled to below 28°C, inoculate under aseptic conditions, and inject 1000 mL of liquid parent seed of shiitake mushrooms into a bot...

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Abstract

The invention provides a liquid fermentation culture medium for shiitake mushrooms. The liquid fermentation culture medium is composed of main raw materials including bran, bean pulp, glucose, monopotassium phosphate, magnesium sulfate and sodium carboxymethylcellulose. The invention further provides a method for producing the shiitake mushrooms through the liquid fermentation culture medium for the shiitake mushrooms with the liquid strain technology. By means of the liquid fermentation culture medium and the method, a traditional method for producing shiitake mushroom strains is changed, the liquid strain technology is adopted in shiitake mushroom strain production, the production efficiency is improved, and the production cost is greatly saved; in addition, the production method is simple in technology, the produced strains are high in use rate, and the pollution rate is low.

Description

technical field [0001] The invention belongs to the field of edible fungus production, and in particular relates to a mushroom liquid fermentation medium and a method for producing mushrooms using liquid strain technology. Background technique [0002] Shiitake mushrooms, also known as mushrooms and mushrooms, are the fruiting bodies of mushrooms of the family Pleurotus, with high nutritional value. Shiitake mushroom is the second largest edible fungus in the world and one of the special products in my country. It is known as "mountain delicacy" among the people. [0003] The protein content of shiitake mushrooms is as high as 18.6%, the fat content is as low as 4.8%, the carbohydrate content is 71%, and the crude fiber content is 9.6%. Group, iron, potassium, provitamin D (converted into vitamin D after sun exposure). [0004] Lentinus edodes has a variety of biological activities and pharmacological effects. Shiitake mushrooms are sweet in taste and flat in nature. They...

Claims

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Application Information

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IPC IPC(8): C05G3/00A01G1/04
Inventor 金文
Owner 井冈山井祥菌草生态科技股份有限公司
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