Method of producing Agrocybe aegerita strains by liquid culture and preservation method of Agrocybe aegerita strain

A technology for liquid culture and tea tree mushroom, applied in the fields of botanical equipment and methods, horticulture, application, etc., can solve the problems of inconvenient mechanization and standardized production, low production efficiency, long cultivation period, etc., and saves bottling and production. Low cost and fast mycelial germination

Inactive Publication Date: 2014-06-25
江西省农业科学院农业应用微生物研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The solid culture method has disadvantages such as long culture period, inconsistent bacterial age, high production cost, low production efficiency, and inconvenient mechanization and standardized production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The present invention provides a kind of method of liquid culture production tea tree mushroom strain, and its process step and process parameter are:

[0025] 1. Liquid culture efficient strain screening

[0026] It is preferred to culture a strain with a large number of bacterial balls, uniform size, and many burrs on the surface, the number of bacterial balls is 200-260 / ml, and the diameter of bacterial balls is 1.0-1.5ml;

[0027] 2. shake flask culture

[0028] ① Shake flask medium formula: glucose 1-3%, corn flour 2-4%, yeast powder 0.2-0.4%, bran 1.0-3.0%, KH 2 PO 4 0.5-1.5%, MgSO 4 0.5-1.0%, PH value is natural.

[0029] ② Shake flask culture medium preparation: wash the shake flask first, then empty it, and the sterilization method is the same as the conventional one. Weigh the raw materials according to the shake flask medium formula, boil the potato slices for 10-15 minutes, filter and take the filtrate, boil the wheat bran for 10-15 minutes, take the...

Embodiment 2

[0042] 1. Liquid culture efficient strain screening

[0043] It is preferred to culture a strain with a large number of bacterial balls, uniform size, and many burrs on the surface, the number of bacterial balls is 200-260 / ml, and the diameter of the bacterial balls is 1.0-1.5mm;

[0044] 2. shake flask culture

[0045] ① Shake flask medium formula: 1% glucose, 4% corn flour, 0.3% yeast powder, 2.0% bran, KH 2 PO 4 0.5%, MgSO 4 0.8%, PH value is natural.

[0046] ② Shake flask culture medium preparation: wash the shake flask first, then empty it, and the sterilization method is the same as the conventional one. Weigh the raw materials according to the formula of the shake flask medium, boil the potato slices for 15 minutes, filter and take the filtrate, boil the wheat bran for 15 minutes, take the filtrate after filtering, heat and dissolve the rest of the raw materials and bottle them together with the filtrate, the filling coefficient of the shake bottle 25%, sterilize...

Embodiment 3

[0059] 1. Liquid culture efficient strain screening

[0060] The preferred liquid culture strains have a large number of bacterial balls, uniform size, and many surface burrs, the number of bacterial balls is 200-260 / ml, and the diameter of bacterial balls is 1.0-1.5mm.

[0061] 2. shake flask culture

[0062] ① Shake flask medium formula: 3% glucose, 2% corn flour, 0.2% yeast powder, 1.0% bran, KH 2 PO 4 1.5%, MgSO 4 1.0%, PH value is natural.

[0063] ② Shake flask culture medium preparation: wash the shake flask first, then empty it, and the sterilization method is the same as the conventional one. Weigh the raw materials according to the formula of the shake flask medium, boil the potato slices for 10 minutes, filter and take the filtrate, boil the wheat bran for 10 minutes, filter and take the filtrate, heat and dissolve the rest of the raw materials and bottle them together with the filtrate, the filling factor of the shake bottle is 20 %, sterilized at a tempera...

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Abstract

The invention provides a method of producing Agrocybe aegerita strains by liquid culture and a preservation method of the Agrocybe aegerita strains. The method includes the steps and process parameters: liquid culture efficient strains are screened, namely the liquid culture strains with many pellets, uniform size and many surface burrs are optimally selected, the amount of culture pellets is 200-260/ml, the pellets are 1.0-1.5mm in diameter, a shake-flask culture formula includes 1-3% of glucose, 2-4% of corn flour, 0.2-0.4% of yeast powder, 1.0-3.0% of wheat bran, 0.5-1.5% of KH2PO4 and 0.5-1.0% of MgSO4, with natural PH value, the culture medium formula for fermenting tank culture of Agrocybe aegerita liquid strains is provided, and process conditions for fermenting tank culture of Agrocybe aegerita liquid strains are established. The key technical problems of production of Agrocybe aegerita strains are solved by the production of Agrocybe aegerita strains by liquid culture; the production cycle is short, production cost is low, fruiting is even after inoculation, and management is facilitated.

Description

technical field [0001] The invention relates to a method for producing tea tree mushroom strains by liquid culture and a preservation method thereof. Background technique [0002] The production of strains is a key link in the production of tea tree mushroom. The production methods of edible fungus strains mainly include solid culture and liquid culture. At present, the culture method of tea tree mushroom strain is mainly solid culture. The solid culture method has disadvantages such as long culture period, inconsistent bacterial age, high production cost, low production efficiency, and inconvenient mechanization and standardized production. The production of edible fungus strains by liquid culture is a multi-factor production technology, which involves strains, materials and formulas, submerged fermentation technology, etc. The process route and process parameters significantly affect the production, quality and benefits of edible fungi. Contents of the invention [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 魏云辉陈庆隆郑立平胡中娥胡丽芳魏小桃程浩
Owner 江西省农业科学院农业应用微生物研究所
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