Method for liquid fermentation cultivation of Pleurotus cornucopiae strain

A liquid fermentation and culture method technology, applied in the directions of botanical equipment and methods, horticulture, application, etc., can solve the problems of inability to meet the needs of large-scale cultivation, large amount of cultivated species, slow growth rate, etc., and achieve good economic benefits. and social effects, easy inoculation, and short production cycle

Active Publication Date: 2012-07-11
湖北康肽药业有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Jiji mushrooms have been produced using solid strains, but solid strains have obvious defects, mainly in the aspects of long growth cycle, inconsistent bacterial age, slow growth rate, large amount of cultivated species, high production cost, etc. Obviously, it cannot meet the needs of large Large-scale cultivation use requirements

Method used

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  • Method for liquid fermentation cultivation of Pleurotus cornucopiae strain
  • Method for liquid fermentation cultivation of Pleurotus cornucopiae strain
  • Method for liquid fermentation cultivation of Pleurotus cornucopiae strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Take about 0.5cm of activated slant parent species 2 Inoculate the small pieces into the liquid culture medium, shake and cultivate the first-level shake flask strains at 25°C, and then transfer the cultured first-level shake flask liquid strains to the second-level liquid strain shake flasks for each treatment , carried out successively at all levels of culture, unless otherwise specified, all levels of shaking flask culture conditions are: 250mL Erlenmeyer flask liquid volume 100mL, inoculum size 5% (V / V), shaker speed 150r / min, 25 ℃ shaking culture 5 ~7d, similarly, the other levels of liquid strains are also cultivated in the same way. The liquid medium formula of Jiji mushroom liquid strain is: nitrogen source, carbon source, KH 2 PO 4 3g, MgSO 4 ·7H 2 O 1.5g, VB 1 10mg, water 1000mL, pH natural, sterilized at 121°C for 20min. The formula of PDA universal medium for slanted culture of Jiji mushroom: 200g potato, 20g sucrose, 1000mL water, natural pH, steril...

Embodiment 2

[0055] Use 10L full-automatic gas-lift stirring fermenter, liquid capacity is 6L, by 5% (v / v) inoculum size, under 26 ℃ of culture conditions, in liquid culture optimum medium (mannitol 30g, yeast extract 10g, corn Powder 10g, KH 2 PO 4 2g, MgSO 4 ·7H 2 (0.5g, pH value 6.0, water 1000mL), carry out liquid fermentation culture, by sampling analysis and recording fermenter system monitoring data, each growth index variation result of the liquid hyphae of Jiji mushroom in the air-lift fermenter is: In the type fermenter, as the fermentation time prolongs, the parameters of reducing sugar content, dry weight of mycelia, density of mycelium balls and diameter of mycelium balls all have a gradual increase and begin to decline on the 6th day of fermentation. trend; but the pH value is the opposite trend, showing a gradual downward trend. When the fermentation reached the 7th day, the dry weight, density and diameter of the mycelial balls of Jiji mushroom in the fermenter decreas...

Embodiment 3

[0057] After the liquid strain was cultivated in the shake flask, directly inoculate the cultivated species culture material (98% of cottonseed hull, 1% of lime, 1% of gypsum, mass percentage, material to water mass ratio of 1% to 750mL glass strain bottle) : 1.2, the pH value is natural); the solid cultivar basically covers the upper part of the strain bottle. After the solid cultivation material is bagged, it is sterilized at 121°C for 120 minutes, and inoculated when it is cooled to 28°C. The inoculation standard is that the solid strains basically cover the top of the strain bag, and the liquid strains cover the top of the culture material evenly with mycelium balls. After inoculation, the cultivation bags are concentrated in a sterilized cultivation room for constant temperature cultivation at 25°C, and the cultivation is carried out by turning over regularly. Comparing the growth speed of liquid strains and solid cultivars on the cultivation material, the result is: the ...

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Abstract

The invention discloses a method for liquid fermentation cultivation of Pleurotus cornucopiae strain, which includes: firstly, inoculating slant mother culture into a 250mL culture bottle containing 120mL of liquid medium, controlling temperature to be 21-27 DEG C, shaking at 90-180rpm, culturing for 5-7 days to obtain primary shaking strain, transferring primary seeds accounting for 5-10% of the inoculation amount to secondary culture liquid for cultivation, transferring cultured secondary shaking liquid strain to tertiary culture liquid for cultivation, and sequentially performing all levels of cultivation to obtain seed broth by the same methods; and secondly, inoculating the seed broth accounting for 5% of the inoculation amount into a 10L fermentation jar containing 6L of fermentation medium for fermentation cultivation, and culturing at 25 DEG C for 5-6 days. The method is characterized by short production cycle, uniform fungus age, low production cost and simplicity in inoculation, the liquid strain is evidently faster than traditional solid spawn in growth speed, the culture bottle can be full of mycelia in 16 days, the time is shortened evidently, the growth speed of the liquid strain by inoculation is increased by 75%, and the method is absolutely applicable to practice of factory production.

Description

technical field [0001] The invention belongs to the technical field of edible fungus cultivation, and in particular relates to a method for liquid fermentation and cultivation of mushroom strains. Background technique [0002] Jiji mushroom (Pleurotus cornucopiae) is also called yellow and white pleurotus, purple spore pleurotus, purple spore oyster mushroom, and Pleurotus japonicus. Jiji mushroom has a good taste, crisp and tender texture, delicious taste and high nutritional value. The protein content is higher than that of ordinary vegetables. It contains eight kinds of amino acids necessary for the human body. Its fruiting bodies are used as medicine to nourish and strengthen, and can treat many diseases. Jiji mushroom is not a classification name, nor a strain name, but a trade name, which refers to a type of oyster mushroom with a harvested fruiting body cap with a diameter of less than 3 cm. Jiji mushrooms have been produced using solid strains, but solid strains hav...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 李冠喜孟德龙温以斌华国栋刘耀鸿刘辉王多明
Owner 湖北康肽药业有限公司
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