Preparation method and application of fungal suspension

A bacterial suspension and fungal technology, applied in the biological field, can solve the problems of cumbersome steps, invisible pollution, time-consuming, etc., and achieve the effect of simple and convenient operation, strong permeability and fast germination

Inactive Publication Date: 2017-02-15
SHENZHEN ZEQINGYUAN TECH DEV SERVICE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this liquefied strain has good vigor, strong permeability, many germination points, and high yield, but because the liquefaction of this technology is a solid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0026] Example 1

[0027] This embodiment discloses a method for preparing a fungus (Pleurotus ostreatus) suspension. The method includes the following steps:

[0028] (1) Preparation of liquid strains: first prepare 300 mL of "corn flour + soybean meal powder" liquid medium, dispense into 250 mL Erlenmeyer flasks, 100 mL per bottle, autoclave at 121°C for 30 minutes. After cooling, inoculate 2-3 pieces of test tube mother seeds the size of soybeans without culture medium, and cultivate on a shaker. The culture conditions: temperature: 20-25°C, rotation speed: 180-220 rpm. Liquid strain can be obtained after 7 days of cultivation;

[0029] (2) Filtration and elution: pass the liquid strain obtained in step (1) through a 10-mesh sieve, and eluted with sterile water three times to obtain 45 g of pure mycelium balls;

[0030] (3) Add water to pulverize: add the pure mycelial pellets obtained in step (2) to a sterile pulverizer, and add 4500 mL of sterile water, pulverize for about 1 min...

Example Embodiment

[0037] Example 2

[0038] This embodiment discloses a method for preparing a fungus (flammulina velutipes) suspension. The method includes the following steps:

[0039] (1) Preparation of liquid strains: first prepare 300 mL of "corn flour + soybean meal powder" liquid medium, dispense into 250 mL Erlenmeyer flasks, 100 mL per bottle, autoclave at 121°C for 30 minutes. After cooling, inoculate 2-3 pieces of test tube mother seeds the size of soybeans without culture medium, and cultivate on a shaker. The culture conditions: temperature: 20-25°C, rotation speed: 180-220 rpm. Liquid strain can be obtained after 7 days of cultivation;

[0040] (2) Filtration and elution: pass the liquid strain obtained in step (1) through a 10-mesh sieve, and eluted with sterile water three times to obtain 45 g of pure mycelium balls;

[0041] (3) Add water to pulverize: add the pure mycelial pellets obtained in step (2) to a sterile pulverizer, and add 4500 mL of sterile water, pulverize for about 1 mi...

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PUM

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Abstract

The invention belongs to the field of biology and particularly relates to a preparation method and application of a fungal suspension. The preparation method includes the steps of 1), preparing a liquid strain; 2), filtering and eluting; 3) adding water and grinding so as to obtain the fungal suspension. The fungal suspension has the advantages of strong mycelia vigor, many germination points, fungus age consistency, short production cycle, small pollution risks, low production cost and good economic benefit. The preparation method has the advantages that the defects of traditional solid strains, liquid strains and existing solid liquefied strains are overcome. The fungal suspension can be used for preparing liquid strains except for production and has a promising market prospect and huge economic value.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to a preparation method and application of a fungal suspension. Background technique [0002] Basidiomycetes have a well-developed mycelium with a septum. At present, basidiomycetes, especially edible fungus strains, mainly exist in two forms: solid strains and liquid strains. Although solid strains are easy to inoculate, they are slow to develop, the culture period is long, and there is a high probability of invisible pollution. Although the liquid strains germinate quickly and the culture period is short, due to the high sugar content, the strains are relatively viscous, the permeability is not good, and the probability of large-scale contamination is high. [0003] At present, in addition to the traditional solid and liquid strains, there is also a special strain - solid liquefied strains. The specific preparation method is as follows: first prepare the liquid bacteria, then...

Claims

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Application Information

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IPC IPC(8): C12N1/14A23L31/00C12R1/645
CPCC12N1/14
Inventor 彭志立黄梦婷陈强
Owner SHENZHEN ZEQINGYUAN TECH DEV SERVICE CO LTD
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