Preparation method of solid liquefied strain of jiji mushroom
A technology of solid liquefied strains and production methods, which is applied in the fields of botanical equipment and methods, fungi, mushroom cultivation, etc. Consistent growth of bacteria, increased yield of fruiting bodies, and neat fruiting
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Embodiment 1
[0021] First, prepare the jitake mushroom strain,
[0022] The preparation method of the solid liquefied bacterial strain of Jiji mushroom comprises the following steps:
[0023] Step 1: Inoculate the first-level liquid bacteria (sterilized by ultraviolet light for 20 minutes before inoculation, inoculate in the ultra-clean workbench, inoculate 4-5 pieces of bacteria with an area of 3mm*3mm in each bottle) in culture bottles, 22- Cultivate statically at 25°C for 2-3 days, the growth of the bacterial block is normal, adjust the rotating speed of the oscillator to 200r / min under the condition of no bacteria, and cultivate for 5-10 days to obtain the liquid species; the liquid species described in step 1 The culture medium is: 1L culture material formula is: 200g of potatoes, 20g of bran, 20g of glucose, 1g of potassium dihydrogen phosphate, and 0.5g of magnesium sulfate. After the culture solution is boiled, it is divided into 1000ml shake flasks, with 200-300ml of culture ma...
Embodiment 2
[0031] The preparation method of the solid liquefied bacterial strain of Jiji mushroom comprises the following steps:
[0032] Step 1: Inoculate the first-level liquid bacteria (sterilized by ultraviolet light for 20 minutes before inoculation, inoculate in the ultra-clean workbench, inoculate 4-5 pieces of bacteria with an area of 3mm*3mm in each bottle) in culture bottles, 22- Cultivate statically at 25°C for 2-3 days, the growth of the bacterial block is normal, adjust the rotating speed of the oscillator to 200r / min under the condition of no bacteria, and cultivate for 5-10 days to obtain the liquid species; the liquid species described in step 1 The culture medium is: 1L culture material formula is: 200g of potatoes, 20g of bran, 20g of glucose, 1g of potassium dihydrogen phosphate, and 0.5g of magnesium sulfate. After the culture medium is boiled, it is divided into 1000ml shake flasks, with 200-300ml of compost in each bottle, and sterilized at 121°C for 30min.
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