59results about How to "Good root quality" patented technology

The invention discloses a grafting method taking masson pine tissue culture seedlings as stocks. The grafting method comprises processes of stock cultivation, scion preparation, seedling grafting and post-grafting management and protection; by taking the masson pine tissue culture seedlings cultivated for 4 to 6 months as the stocks and collecting bud sticks germinating on stock plants as scions, grafting is performed by adopting a split grafting method in December to January or in March to April; grafted seedlings are cultivated under the conditions of suitable light and humidity, and water and fertilizer management and pest control are performed by a conventional method. According to the grafting method disclosed by the invention, the masson pine tissue culture seedlings with developed roots, vigorous metabolism and rapid growth are cultivated through a tissue culture technological means to serve as the stocks, therefore not only can the seedling period of the stocks be shortened and the grafting survival percentage be increased but also the genetic gain of an excellent variety is ensured; adverse effects brought by the fact that heterogeneous-stock grafting is performed by taking slash pines as the stocks widely in masson pine grafting in production at present are effectively avoided, and the working effect of masson pine tissue culture stocks is greatly promoted; the grafting method has a broad application prospect, and has better economic benefit, social benefit and ecological benefit.

The invention discloses a method for quick rooting through cuttage of chrysanthemums. Specifically, the method includes the following steps of 1 preparing a cuttage bed, 2 preparing cuttings, 3 carrying out cuttage and 4 carrying out seedling growing and management, wherein in the step 1, matrixes sprayed with sterilizing agents are placed in a plurality of seedling growing hole trays; in the step 2, the cuttings are cleared up, the lower portions of the cuttings are cut and sequentially placed in the sterilizing agents and rooting agent solutions, and the rooting agent solutions are a mixed solution composed of indolebutyric acid, a multiwalled carbon nanotube and water; in the step 3, the center of each hole of each seedling growing hole tray is vertically provided with a hole in an inserted mode with a bamboo stick, and then the processed cuttings are vertically cut in the holes and watered; in the step 4, all-day shading treatment is conducted on chrysanthemum seedlings at the callus forming period of seedling stages, shading treatment only needs to be carried out when sunlight is intensive at the callus complete forming period of the seedling stages, and the sterilizing agents need to be sprayed onto the chrysanthemum seedlings at the root system forming period of the seedling stages. The method has the advantages that the cuttings can be promoted to quickly root, rooting periods are short, orderly rooting is achieved and rooting quality is good.

The invention relates to asparagus parent breeding method, mainly solving the problems that rooting rate is low, root quality is poor and the survive rate of the regeneration plant is low in asparagus organ tissue culture process. The method includes the process that high quality asparagus stem section is selected, culture is carried out in culture medium solution, and asparagus seedling meeting transplant standard is bred. The culture medium solution is confected by the way that hormones corresponding to various culture stages are added on the basis of the basic culture medium solution, the culture includes induction culture, propagation culture, rooting pre-differentiation culture and rooting culture sequentially. Compared with the existing organ culture, the invention has high propagation rate, rooting rate reaches 100%, root system quality is good, and differentiation rate of strong fleshy tap root is improved to 96%, thus ensuring survival rate after transplantation.

The invention discloses a method for obtaining virus-free sweet potato seedlings through high-temperature-variable temperature processing, and belongs to the technical field of virus-free sweet potatoseedling culturing. According to the method, high-temperature processing of 40-45 DEG C is conducted on rooted sweet potato seedlings for five hours every day, and the processing is conducted continuously for 30-40 days. The method has the advantages that the passivating effect on viruses is high, a stripped stem tip has a long length, the stripping is easy, the efficiency is high, the growth ofan adventitious bud is fast, the rooting quality of regenerating seedlings is good, the seedlings are strong, and the survival rate is high.

The invention relates to an external rooting culture method of endangered relict tree species yew seed tissue culture seedlings. The method comprises the following steps: disinfecting surfaces of yew seeds, inoculating the disinfected yew seeds to an MS medium to make 50% of the seeds germinate 30d later, taking terminal buds of aseptic seedlings with hypocotyl with the length of 1.5cm as explants, inducing cluster buds in a medium I to make the induction rate reach 80%, cutting off new terminal buds and stem segments with lateral bud, subculturing in a medium II to obtain subcultured buds with the subculture multiplication coefficient of 7.5, repeatedly cutting off subcultured bud terminal buds and bud segments, and carrying out propagation and propagation expanding in the medium II to obtain a large amount of propagated subcultured buds; separately cutting off 4-6cm length terminal buds, carrying out rapid dipping treatment in a 500mg.L<-1> alpha-naphthylacetic acid solution, transplanting the obtained buds to an aperture disk, carrying out external rooting, and covering with a plastic aperture disk cover to carry out moisture retention; and allowing the rooting treatment to be carried out for 60d to make the rooting rate reach 64%, and transplanting rooted seedlings to a nursery, wherein the transplanting survival rate reaches 95%. The method is simple and easy, allows tissue culture rapid breeding of internal difficult-rooting tree species yews to be successfully completed, can be applied in large-scale industrial production of the endangered relict tree species yews, and solves the problem of tube asexual breeding of the yews.

The invention discloses a grape cutting root promoting method. The method is characterized by comprising the steps that cuttings obtained from mature and robust branch tendrils of a high-quality single plant are soaked with a traditional Chinese medicine nutritional solution for 8 hours to 12 hours till water at cutting wounds is sufficient, the lower ends of the cuttings are dipped in a 1,000 mg/L indoleacetic acid solution, the cuttings are cut into plastic bags containing culture soil, one bud at the upper end of each cutting is left outside the corresponding plastic bag, water is added to enable the relative humidity of the soil to reach 85% to 90%, and the cuttings are placed in a cold frame and covered with film. According to the method, the rooting rate of grape cuttings can be significantly increased, the rooting quality of the grape cuttings can be significantly improved, and the step of repeatedly supplementing water in the root promoting process is omitted.

The invention discloses a cutting propagation method for lobelia erinus. Annual branches are sheared form lobelia erinus plants and sheared into cutting slips with leaf stems, wherein the length of the cutting slips is 5-6 cm, 3-4 knots are kept on each cutting slip, one axillary bud and 1-2 small leaves are kept on each upper end knot, the position, away from the upper end knot of each cutting slip, of an upper cutting edge of the cutting slip is sheared into an obtuse-angle inclined face, and the position, away from the lower end knot of each cutting slip, of a lower cutting edge of the cutting slip is sheared into an acute-angle inclined face; the cutting slips are treated with a rooting powder solution; then, seedling-culturing land is covered with a cutting medium with the thickness of 8-10 cm; next, cutting and watering are carried out; an arched shed is constructed, temperature is controlled from 23 DEG C to 28 DEG C, leaf and seedling fertilizer starts to be applied and dressed when 3-4 leaves of each seedling grow out, and meanwhile illumination intensity needs to be appropriately increased in the growth period of the seedlings. The survival rate of cutting propagation is high, and rooting quality is good.

The invention relates to a cutting seedling-raising method of Pyrus betulifolia Bunge. The method comprises the following steps: step (1): preparing a cutting seedling bed; step (2): pretreating cuttings; step (3): performing cutting; step (4): performing management after cutting; and step (5): performing seedling-hardening and transplanting. The cuttings obtained by the method have good rooting effects, relatively many lateral roots, and good root system quality, and obtained seedlings have a high pass rate, and neat and consistent growth conditions, so that the cutting survival rate of Pyrusbetulifolia Bunge is effectively improved, and a solid foundation for an application of the cutting technology in the actual production of Pyrus betulifolia Bunge is laid.

The invention provides a seedling culturing method of pitaya, and belongs to the technical field of agricultural planting. The method comprises management of cutting seedlings and management of seedlings. A specially prepared cutting matrix is adopted during cutting. Cutting stems are immersed in a rooting solution and then cut; the rooting solution is prepared from the following components with the following concentration: ABT rooting powder I with the concentration of 3.0 to 4.0 mg/mL, diethyl aminoethyl hexanoate with the concentration of 1.0 to 2.0 mg/mL, jasmonic acid with the concentration of 0.80 to 1.0 mg/mL and chlorethyl alcohol with the concentration of 0.22 to 0.28 mg/mL. According to the method, the soil is further improved through specially prepared base fertilizer during the management of the seedlings, so that the fertility of the soil is improved. The method greatly increases the rooting rate of pitaya cutting; the obtained seedlings are more robust in root growth; in the seedling stage, pitaya seedlings have better growth vigour; and a good foundation is laid for high yield of subsequently planted pitaya.

The invention discloses an air-layering rooting and seedling-growing promoting method for mulberries. The air-layering rooting and seedling-growing promoting method for mulberries includes the following steps of branch selecting and girdling, mud packaging, rooting cultivating and in-vitro seedling growing; rooting is induced by adding rooting powder into packaged mud and the moisturizing measure, and the survival seedling rate is increased. The air-layering rooting and seedling-growing promoting method for mulberries is easy to operate; mud is covered with films, moisture in mud is prevented from being evaporated, moist of matrixes is kept, and rooting is further facilitated; limitation of varieties, tree shapes and branches of mulberries do not exist, and almost all mulberries can root and grow seedlings with the method; cultivated roots of mulberry seedlings are good in development, particularly, main roots are sturdy, lateral roots are developed, young seedlings are robust, the transplanting survival rate can reach 80% or above, and the environmental adaptability is high; the rooting time is short, and the rooting quality is good; the speed and the quality of vegetative propagation of mulberries are greatly increased, and a guarantee is provided for good-seed breeding and popularization. In a word, the rooting quality of mulberries can be rapidly, efficiently and greatly improved with the method, the seedling survival rate and the transplanting survival rate of vegetative propagation of mulberries are increased.

The invention relates to growth promoting rhizobacteria of a strain of semen euryales. The classification and the name of the growth promoting rhizobacteria of the strain of semen euryales are baclicus lincheniformis Q67; the growth promoting rhizobacteria of the strain of semen euryales is preserved in the China Center for Type Culture Collection; the preservation number is CCTCC NO: M2014652. By using the growth promoting rhizobacteria Q67 of the semen euryales, which is provided by the invention, the growth and the development of seedlings of the semen euryales can be obviously promoted, and the content of available nitrogen in a soil environment in which the semen euryales grows is improved. The invention also relates to the application of the growth promoting rhizobacteria of the semen euryales to the preparation of a microbial inoculum, and as results show, the microbial inoculum can obviously improve the germination rate of seeds of the semen euryales.

The invention provides an out-bottle one-step plantlet developing method for rootless test-tube cunninghamia lanceolata seedlings. The out-bottle one-step plantlet developing method includes the steps that by reducing the inoculum density of cunninghamia lanceolata clumpy buds, the illumination intensity of the subculture seedlings in the culturing period is improved, the illumination time of the subculture seedlings in the culturing period is prolonged, the culturing cycle is prolonged, and the subculture seedlings high in lignification degree are obtained; the subculture seedlings are cut, after rooting processing is carried out on the subculture seedlings through rooting agent, the subculture seedlings are transplanted to a sterilized non-woven fabric container cup to be cultured, seedling management is carried out, and cunninghamia lanceolata plantlets are obtained. By means of the out-bottle one-step plantlet developing method, the culturing steps for tissue cultured seedlings are simplified, the links of test-tube seedling in-bottle rooting inducing, seedling hardening and the like are omitted, the rootless test-tube cunninghamia lanceolata seedlings are directly transplanted to nursery gardens for transplanting, the subculture seedlings obtained after subculture enrichment culturing can further develop to be the plantlets, the time and the cost for the steps of test-tube seedling in-bottle rooting, seedling hardening and the like are effectively saved, and the good economic benefits, the good social benefits and the good ecological benefits are achieved.

The invention discloses a method for promoting rooting of alfalfa tissue culture seedlings. The method comprises the following steps: A, preparing a solid rooting culture medium (1) in a hollow cylindrical shape; B, inoculating the upper end surface of the solid rooting culture medium (1) with alfalfa seedlings obtained by tissue culturing, and enabling the roots of the alfalfa seedlings to grow downwards in a semi-hanging manner along the inner wall of the solid rooting culture medium (1) in the hollow cylindrical shape; and C, sealing by a sealing membrane after inoculating, performing rooting culture in a tissue culturing chamber, and performing seedling hardening and transplanting 1-2 weeks later when the roots of the alfalfa seedlings grow to over 5cm. According to the method for promoting rooting of alfalfa tissue culture seedlings, the alfalfa tissue culture seedlings are subjected to semi-hanging rooting culture through the solid culturing medium with a specific structure for the first time, and an efficient alfalfa rooting culture system is established by selecting appropriate rooting culture medium and rooting agent. The problems that the alfalfa tissue culture seedlings are slow in rooting, fewer in side roots, not developed in root system, low in transplanting survival rate and the like can be solved.

The invention discloses a novel acacia melanoxylon tissue culture seedling rooting method. The method includes the steps that small holes with the depths of 1.0-1.5 cm are uniformly formed in a watered transplanting substrate through inoculation tweezers in a pricking mode firstly; an acacia melanoxylon rootless stem bud cultured through a strong seedling is taken out of a tissue culture bottle, culture media of a base portion are washed clean with tap water, then the acacia melanoxylon rootless stem bud is cut off from the base portion with an inoculation cutter, and leaves are cut off until 3-5 leatherleaves are left around an apical bud through the inoculation cutter; the acacia melanoxylon rootless stem bud is put in a rooting accelerant to be immersed for a certain period of time, then a small seedling is clamped through the tweezers to be inserted into the transplanting substrate, the transplanting substrate is pressed to be compact, and the exposed apical bud and the leaves exposed are 1.5-2.0 cm away from the upper portion of the transplanting substrate; ultimately, a culture box is placed in a domestication chamber where lighting intensity and lighting time are set. According to the method, root quality is greatly improved, loss caused by pollution is reduced, the survival rate of transplanted seedlings is improved, the seedling cycle is shortened due to the fact that rooting, domestication and transplanting are combined, and production cost is greatly saved.

The invention discloses a grafting method of ruby green pomelo. The method includes: grafting scion of semi-ligninized ruby green pomelo with a transverse diameter of 0.6-0.8 cm on a rootstock with atransverse diameter of 1.5-2.5 cm by cutting grafting, tightly wrapping with a grafting film, shading and cultivating, when the scion is drawn out of the new shoots, cutting the grafting film beside the new shoots, removing sprouting tillers on the rootstock in time; and cultivating the new shoots under full light after they are mature. The rootstock is a seedling of lime or honey pomelo, or the lime is taken as a base stock, and the honey pomelo is taken as an intermediate stock for grafting. According to the method, through strict stock preparation and cutting scion treatment, callus can berapidly generated between the stock and the scion, which has high survival rate, good growth quality after survival, strong plants, and significantly shorter grafting time. The method provides strongtechnical support for accelerating rapid development of the ruby green pomelo industry and using lime with low planting value, is particularly suitable for large-scale production and scientific research needs, and has broad application prospects.

The invention discloses a seedling growing container comprising a plug tray with a first opening and a second opening at two ends; a built-in earthen bowl body which is installed inside the plug tray, wherein a blind end is formed by one end, close to the first opening, of the earthen bowl body, and a third opening is formed by the other end, close to the second opening, of the earthen bowl body; cuttage substrate, wherein the built-in earthen bowl body is filled with the cuttage substrate; a cuttage tray which is installed on the second opening in a clamping mode, wherein the cuttage tray is provided with a cuttage hole for inserting hardwood branches. The invention discloses a hardwood branch cuttage device comprising a water filtering device and the seedling growing container, wherein the seedling growing container is installed on the water filtering device with the first opening facing upwards and the second opening facing downwards. The invention also discloses a hardwood branch inverted cuttage method comprising following steps: providing a hardwood branch cuttage device; providing a hardwood branch and inserting the hardwood branch from the cuttage hole to the cuttage substrate; placing the hardwood branch cuttage device inserted with the hardwood branch in a cuttage cultivation device; water spray maintenance is performed according to settings; transplanting with earthen bowls can be carried out until the seedlings of the hardwood branch grows well with good rooting.

The invention relates to a needle-leaved tree water culturing technology, in particular to a pinus-contorta needle-leaved-bundle water culturing technology. The collecting time of needle-leaved bundles is the mid-December, storage time is 30 days, hormones include IAA of 80 mg/L to 85 mg/L and IBA of 80 mg/L to 85 mg/L, and soaking time is 24 hours; a nutrient solution in the optimal formula is prepared from 40 mg/L of boric acid, 30 mg/L of ammonium nitrate, 30 mg/L of VB1 and 20 mg/L of monopotassium phosphate, water culturing temperature is 22 DEG C, water culturing humidity is 80%, water culturing illuminance is 2,100 Lx, and illumination is 14 h/d. By means of needle-leaved bundle treatment, the formula of the nutrient solution and the water culturing environment conditions, the rooting time of the pinus-contorta needle-leaved bundles is shortened, the rooting number is increased, the length of root systems is increased, and the obvious effect of promoting rooting of the needle-leaved bundles is achieved. Asexual propagation ways of pinus contorta are increased, and a feasible scheme is provided for industrial seedling growing of the pinus contorta.

The invention belongs to the technical field of plant breeding, and particularly discloses an efficient cutting propagation and seedling raising method for bauhinia corymbosa, which comprises the following steps: 1, preparation of a substrate; 2, selection of cutting slips; 3, rooting treatment; 4, cuttage and management; 5, rooting and transplanting; and 6, strong seedling culture. The method is easy and convenient to operate and low in cost, the problem that the cutting slips of bauhinia corymbosa are prone to infection and black rot is well solved, the cutting rooting effect is greatly improved, and the rooting rate reaches up to 86%; the growth cycle is relatively short, the breeding efficiency is high, a large number of high-quality container seedlings can be rapidly obtained, and the vigorous requirements of landscaping and ecological restoration markets are met.

The invention discloses a method of raising seedlings by hardwood cuttage for Broussonetia papyrifera. According to the technical scheme, the method comprises 8 steps: (1) Stems with axillary buds which are swollen and waiting to germinate in the middle and upper part of maternal branches are clipped in May as cutting materials and the thickness of the stems should be one centimeter above. (2) The cutting materials are cut into cuttings with a length of 14 to 16 centimeters and 3 or more axillary buds are required. (3) The lower part of 1.5 to 2 centimeters of the cuttings is dipped in 120ppmABT number 1 rhizogenous powder solution for 1.5 to 5 hours. (4) A cutting substrate and a seedling bowl are sterilized by disinfectant on the day before cutting. (5) The dipped cuttings are inserted into the substrate of the seedling bowl with a depth of 2 to 3 centimeters. (6) The substrate is kept moist and thoroughly watered at one time while watering and water seepage from a drain hole at the bottom of the seedling bowl is taken as a standard. (7) After cutting and rooting, the quick-acting fertilizer is applied to the substrate once every 7 to 10 days. (8) When roots wind well, the cuttings should be transplanted. The method of raising seedlings by hardwood cuttage for Broussonetia papyrifera not only has high cuttage rooting rate, but also has high rooting quality. Rooting and shooting are synchronized, which is beneficial to early growth and rapid development.

The invention discloses a fruit stem rooting agent. The fruit stem rooting agent is prepared from yellow soil, indole-3-butyric acid potassium, sodium alpha-naphthyl acetate, GGR, sphagnum moss leaching liquor, red floating powder, alligator weed powder, white sugar, pine needle powder, revolution, lushan mountain barberry root powder, indoleacetic acid, magnesium chloride hexahydrate, sodium bicarbonate, calcium hydroxide, activated carbon, citric acid, myristic acid, sodium thiosulfate, glyceryl tristearate, sucrose ester, sodium tartrate, sodium sorbate, lignin, thiabendazole, fiveleaf gynostemma herb, rhizoma bletillae glue, tea polyphenol and ethylhexyl ferulate. The invention also provides a preparation method of the fruit stem rooting agent. The prepared fruit stem rooting agent is strong in rooting capability, can promote fruit stem rooting and improve the fruit stem rooting efficiency and quality, and can inhibit bacteria breeding and prevent fruit stem root rotting in the rooting process.

The invention discloses a method for promoting rapid germination of nitraria tangutorum seeds. The method comprises the following steps of collecting the nitraria tangutorum seeds, removing surface pulp, conducting washing and airing, soaking the nitraria tangutorum seeds in warm water, filling the nitraria tangutorum seeds into a polyethylene bag, tightening a bag opening, conducting slight rubbing back and forth on a flat wood plate or tabletop for 5-10 times, conducting seed covering for 1-2 hours at 30 DEG C, paving the seeds in a tray, covering the tray with a wet towel for 30 minutes, putting the seeds into a MnSO4+ gibberellin solution for soaking, fishing out the seeds, washing the seeds with clear water to be clean, pouring the seeds into a culture dish in which double-layer wet filter paper is laid, additionally adding three layers of wet gauzes at the bottom of the culture dish by taking a gauze wrapping the seeds as a first layer, and finally putting the culture dish into an incubator together, and conducting culturing under the temperature changing condition of 17-27 DEG C. By means of the method, the germination rate of the nitraria tangutorum seeds can be remarkablyincreased, the germination rate within 10 days reaches about 81%, germination is tidy, meanwhile, the root system quality of seedlings in the later period can be improved, and the survival rate of theseedlings is increased.