Novel acacia melanoxylon tissue culture seedling rooting method

A technology of tissue culture seedlings and a new method, which is applied in the field of rooting of Acacia blackwood tissue culture seedlings, can solve problems such as shortening the rooting time, and achieve the effects of shortening the seedling cultivation cycle, saving production costs, and reducing losses

Inactive Publication Date: 2014-07-23
地缘(厦门)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] The development of plant tissue culture and rapid propagation technology has gradually matured so far, but there are still many problems, especially the research on tissue culture and rapid propagation of Acacia blackwood has been at a stagnant stage in recent years, and it has not been possible to find an eff

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  • Novel acacia melanoxylon tissue culture seedling rooting method
  • Novel acacia melanoxylon tissue culture seedling rooting method
  • Novel acacia melanoxylon tissue culture seedling rooting method

Examples

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Example Embodiment

[0030] detailed description

[0031] The embodiments of the present invention will be described in detail below. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals indicate the same or similar elements or elements with the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary, and are intended to explain the present invention, but should not be construed as limiting the present invention. If specific techniques or conditions are not indicated in the examples, the procedures shall be carried out in accordance with the techniques or conditions described in the literature in the field or in accordance with the product specification. The reagents or instruments used without the manufacturer's indication are all conventional products that are commercially available.

[0032] The Acacia blackwood tissue cultured seedlings were derived from the Acacia blackwood ...

Example Embodiment

[0033] Example 1: Acacia blackwood tissue culture seedlings take root

[0034] Operation method: First, poke the transplanting substrate (a mixture of grass charcoal soil+sawdust+perlite, volume ratio 6:3:1) evenly with inoculation tweezers to form one by one about 1.0~1.5cm deep. Small holes. Take the Acacia blackwood rootless shoots that have been cultured with strong seedlings from the tissue culture bottle, wash the base medium with tap water, then cut the Acacia blackwood shoots from the base with an inoculating knife, and then cut the leaves with an inoculating knife 3~5 feather leaves are left near the apical bud, then use tweezers to insert the seedling skewer into the matrix and compact the matrix to expose the apical bud and leaf 1.5~2.0cm, and finally place the culture box at 90~135μmol·m -2 ·S -1 , Just need to cultivate in the acclimation room with 12~16h light time.

[0035] After cutting, pay attention to the following points: 1. Spray the surface of the substrate ...

Example Embodiment

[0036] Example 2: Selection of different experimental conditions

[0037] A. The effect of different transplanting media on the open rooting of Acacia nigra tissue cultured seedlings: Consider 4 substrates: ① turf soil; ② turf soil + sawdust (2:1); ③ turf soil + perlite (3:1) ④ Peat soil + sawdust + perlite (6:3:1). The rootless shoots of Acacia blackwood are not treated with any rooting promoter, and the culture light intensity is 90μmol·m -2 ·S -1 , The cultivation photoperiod is 12h / 12h (light / dark).

[0038] Through experiments, it is concluded that the best transplanting substrate for open rooting of tissue cultured seedlings of Acacia blackwood is turf soil + sawdust + perlite (6:3:1).

[0039] B. The effect of different water content of the transplanting substrate on the open rooting of Acacia blackwood tissue cultured seedlings: tap water with a volume ratio of 1:14, 1:12, 1:10, and 1:8 to the substrate until the water Mix well with the substrate, take the soil evenly in e...

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Abstract

The invention discloses a novel acacia melanoxylon tissue culture seedling rooting method. The method includes the steps that small holes with the depths of 1.0-1.5 cm are uniformly formed in a watered transplanting substrate through inoculation tweezers in a pricking mode firstly; an acacia melanoxylon rootless stem bud cultured through a strong seedling is taken out of a tissue culture bottle, culture media of a base portion are washed clean with tap water, then the acacia melanoxylon rootless stem bud is cut off from the base portion with an inoculation cutter, and leaves are cut off until 3-5 leatherleaves are left around an apical bud through the inoculation cutter; the acacia melanoxylon rootless stem bud is put in a rooting accelerant to be immersed for a certain period of time, then a small seedling is clamped through the tweezers to be inserted into the transplanting substrate, the transplanting substrate is pressed to be compact, and the exposed apical bud and the leaves exposed are 1.5-2.0 cm away from the upper portion of the transplanting substrate; ultimately, a culture box is placed in a domestication chamber where lighting intensity and lighting time are set. According to the method, root quality is greatly improved, loss caused by pollution is reduced, the survival rate of transplanted seedlings is improved, the seedling cycle is shortened due to the fact that rooting, domestication and transplanting are combined, and production cost is greatly saved.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture rapid propagation. In particular, it relates to a new method for rooting tissue-cultured seedlings of Acacia acacia. Background technique [0002] The development of plant tissue culture and rapid propagation technology has gradually matured so far, but there are still many problems, especially the research on tissue culture and rapid propagation of Acacia blackwood has been at a stagnant stage in recent years, and it has not been possible to find an effective method that can improve The quality of the tissue-cultured seedlings can shorten the period of seedling cultivation and reduce the cost; and the rooting of the tissue-cultured seedlings of Acacia blackwood also needs to improve the quality of the root system, shorten the rooting time, and improve the survival rate of transplanting. Contents of the invention [0003] The purpose of the present invention is to provide a new met...

Claims

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Application Information

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IPC IPC(8): A01G31/00
Inventor 刘丽林文革
Owner 地缘(厦门)生物科技有限公司
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