Out-bottle one-step plantlet developing method for rootless test-tube cunninghamia lanceolata seedlings

Abstract

The invention provides an out-bottle one-step plantlet developing method for rootless test-tube cunninghamia lanceolata seedlings. The out-bottle one-step plantlet developing method includes the steps that by reducing the inoculum density of cunninghamia lanceolata clumpy buds, the illumination intensity of the subculture seedlings in the culturing period is improved, the illumination time of the subculture seedlings in the culturing period is prolonged, the culturing cycle is prolonged, and the subculture seedlings high in lignification degree are obtained; the subculture seedlings are cut, after rooting processing is carried out on the subculture seedlings through rooting agent, the subculture seedlings are transplanted to a sterilized non-woven fabric container cup to be cultured, seedling management is carried out, and cunninghamia lanceolata plantlets are obtained. By means of the out-bottle one-step plantlet developing method, the culturing steps for tissue cultured seedlings are simplified, the links of test-tube seedling in-bottle rooting inducing, seedling hardening and the like are omitted, the rootless test-tube cunninghamia lanceolata seedlings are directly transplanted to nursery gardens for transplanting, the subculture seedlings obtained after subculture enrichment culturing can further develop to be the plantlets, the time and the cost for the steps of test-tube seedling in-bottle rooting, seedling hardening and the like are effectively saved, and the good economic benefits, the good social benefits and the good ecological benefits are achieved.

Description

technical field [0001] The invention belongs to the technical field of plant vegetative propagation, and relates to a method for growing seedlings of fir rootless test tube seedlings in one step outside a bottle. . Background technique [0002] Chinese fir (Cunninghamia lanceolata) is the most widely cultivated, fast-growing, and high-economic timber species in the Yangtze River Basin and south of the Qinling Mountains. As the country attaches great importance to the construction of timber strategic reserve bases and the construction of ecological civilization, forestry development has attracted much attention. As one of the main fast-growing timber tree species in southern my country, fir has significantly improved its status in the development of forestry industry. In order to promote the rapid development of the Chinese fir industry, the effective application of improved varieties and methods is the key, and improved varieties are the basis and premise for the smooth dev...

AI Technical Summary

Problems solved by technology

However, compared with the seedlings, the production technical requirements and production costs of fir tissue culture seedlings are higher, and mass production consumes more material and financial resources.

At present, there are still shortages in the market for Chinese fir seedlings in short supply, the materials of Chinese fir seedlings urgently need to be developed, popularized and applied, and the breeding cost of Chinese fir tissue culture seedlings is high and the cycle is long.

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