86results about How to "High degree of lignification" patented technology

The invention discloses a technique for grafting wild Chinese walnut, which comprises: cutting the fracture of a wild walnut or wingnut stock to form an inclined face (1); cutting off 3 to 5 centimeters of skin (2) of the upper end of the higher side of the inclined face (1) to form a 'V' shape (9); cutting off skin (5) in the direction of a bud on a cion branch; peeling the pulp skin layer (3) of the 'V' shape (9) of the stock to separate the pulp skin layer (3) from xylem (4); inserting the inclined plane (6) of a cion from top down while keeping the cion tightly adhering to the xylem (4) of the stock inward; sleeving the whole saw bite on the stock from the joint of the stock and the cion by using an agricultural mulching film; exposing cion branch; and fastening tightly. When the grafting technique is adopted, the operation is simple, the cost is low, and by using wild resources, the grafting survival rate is increased and fruiting is early.

The invention discloses a method for rooting and thickening of fructus momordicae tissue culture seedlings and relates to the field of agricultural breeding methods. The method comprises the following steps: induction culture of tissue culture seedlings, multiplication and successive transfer culture of the tissue culture seedlings, thickening and rooting culture of fructus momordicae tissue culture seedlings, hardening-seedling of the thickened and rooted tissue culture seedlings and transplanting for survival. The formula of a thickening and rooting medium adopted by the method has a better synergistic effect, can achieve the purposes of thickening and rooting, meets the transplant requirement and has strong adaptability to the outside environment, thereby improving the transplant survival rate. The tissue culture seedlings obtained by adopting the method are thick and strong, high in disease resistance and lignification degree and can reach the out-planting transplant survival rate of more than 98%.

The present invention relates to the technical field of Luo Han Guo cultivation, in particular to a method for cultivating Luo Han Guo. The branches are collected and sterilized, and the stem apex meristem is cut out for subculture; the seedlings are selected and quickly dipped in IBA, and the lower 1/4 of the seedlings are inserted into the Between two pieces of filter paper, clamp the filter paper with two aluminum alloy bedding bars, and fix the aluminum alloy beading bar with a small clip, then hang the aluminum alloy beading bar on the two walls of the rooting incubator for rooting cultivation, and the rooting incubator contains a box volume of 1 /4 or 1/2 MS culture solution; before transplanting, apply quicklime to the planting site, and then apply decomposed farmyard manure, place the seedlings in the planting nest and pour enough water for rooting, after 7 days, wet the fertilizer once a week, When the Luo Han Guo seedling grows more than 3 true leaves, remove the film covered on the Mirs, and apply organic compound fertilizer, the method of the present invention obtains transplanted seedlings, the stems are strong, the rooting rate is high, the quality is good, and the survival rate of field transplantation is high. High and adaptable.

The invention discloses a xanthoceras sorbifolia culture method, which relates to the xanthoceras sorbifolia rapid propagation technology and belongs to the technical field of plant tissue culture. The xanthoceras sorbifolia culture method solves the problem of phenomenon of one fruit of thousands of flowers of the xanthoceras sorbifolia. Therefore, the xanthoceras sorbifolia has high seedling differentiation rate, high propagation speed and high seedling yield. The xanthoceras sorbifolia realizes the micropropagation at the speed of several times each month, and the resource regeneration and the utilization of the micropropagation are reached through tender stem or stem tip culture. The xanthoceras sorbifolia culture method has the beneficial effects that the seedling development is healthy and strong, the planting survival rate is high, the seedling raising is early, the growth period is long, the seedling lignification degree is improved, the seedling cold resistance and overwintering capability is enhanced, the seedling raising time is short, the cost is low, and the seedling yield in the unit area is high.

The invention discloses a g rafting cultivation method suitable for apple dwarf interstock seedlings in Alpine land. A malus robusta seedling sowed in the first year is selected as a base stock, an SH-line dwarf stock seedling grafted on the base stock is selected as a dwarf interstock seedling, each seedling needs to be full in bud tip, high in lignification degree and with diameter thickness being 0.5-1.2cm, and grafting survival rate is high. A T-shaped bud grafting method is adopted for grafting, and then conventional management like stock cutting, sprout removal, weeding and soil loosening, fertilizing and pest and disease prevention and control is performed on grafted seedlings. Dwarf interstock apple seedlings after successful grafting have obvious dwarf effect. The SH-line dwarf stock is used as an interstock, is highly cold-resistant and can resist low temperature of minus 36 DEG C, thereby being suitable for high-cold high-altitude regions; the dwarf interstock apple seedlings are high in flexibility, windproof, uvioresistant, extremely high in affinity and unobvious in big-small feet phenomena of the base stock and variety seedlings after grafting; fruiting period is advanced, the seedlings can bear fruit in the third year, fruit coloring rate is increased by 25%, and commodity rate is increased by 25%; orchard establishment cost is low.

The invention relates to the technical fields of plant molecular biology and genetic engineering, and provides a pear laccase gene PbLAC1, the nucleotide sequence of which is shown in SEQ ID NO. 1. The invention also provides a plant over-expression vector, wherein the plant over-expression vector comprises pMD-18T-PbLAC1 plasmid constructed by PbLAC1. The invention also provides a genetically engineered host cell comprising a PbLAC1 gene sequence. The invention also provides the application of the pear laccase gene PbLAC1 in regulating plant lignin synthesis and cell wall development. The pear laccase gene PbLAC1 of the present invention provides new evidence for perfecting the plant lignin synthesis pathway, has important theoretical and practical significance for improving fruit qualityand other key agronomic characteristics of crops, and also provides a new way for regulating the content of pear stone cells by genetic engineering.

The invention relates to the technical field of plant growth regulators, and particularly discloses a nursery stock growth promoting and thickening agent. The nursery stock growth promoting and thickening agent is formed by mixing the following raw materials, by weight, 20 percent to 40 percent of chlormequat chloride, 0.1 percent to 0.2 percent of alpha-pimacol, 0.05 percent to 0.1 percent of indolebutyric acid potassium, five percent to 10 percent of monopotassium phosphate and the balance fillers. The nursery stock growth promoting and thickening agent can promote the growth of nursery stocks well, so that the nursery stocks are thick and strong in stem, developed in root system, high in lignification degree and reasonable in seedling height, and the wind resisting capacity and other adversity tolerating capacity of the nursery stocks are improved.

The invention relates to a tree summer implanting method in drought and half-drought regions, wherein phytohormone is utilized to make the nursery stock enter dormant state, the method comprises the steps of, choosing nursery transplant, spraying plant growth hormone medicament, after the nursery-grown plant dormancy, extracting sprouts rapidly through normal procedures and packaging the soil with plastic, irrigating with ferrous sulfate aqueous solution of 0.1% concentration, sprinkling foliage 1-3 times with 5% KH2PO3 solution.

The invention discloses a cultivation method of sound seedlings of blueberry tissue culture seedlings. The cultivation method comprises the following steps: (1) blending a base material; (2) pre-treating bottle seedlings; (3) inserting the seedlings; (4) disinfecting; (5) sealing by a film and culturing; and (6) removing the film and carrying out post-period management. In the step (3), self-made rooting liquid is utilized and cuttage is realized by adopting a manner of avoiding generation of aerial roots; in the step (2) and the step (5), light illumination is realized by adopting an LED (Light Emitting Diode) light source; in the step (6), the post-period management is realized by adopting a specific step and the sound seedlings of the blueberry tissue culture seedlings are produced; and a relatively high survival rate of young blueberry tissue culture seedlings can also be guaranteed when the young blueberry tissue culture seedlings are directly planted into a large field, without the need of a nutrition pot sound seedling heeling-in period. By adopting the cultivation method disclosed by the invention, the seedling cultivation period can be shortened and a middle production link is reduced; and the transportation cost and the seedling cultivation cost are reduced.

The invention discloses an edible mushroom culture medium and a preparation method thereof. A formula of the culture medium comprises the following ingredients: 50 to 60 parts of baijiu vinasse, 20 to 30 parts of bagasse, 5 to 15 parts of bran, 1 to 2 parts of plaster stone, 1 to 2 parts of coal, 1 to 2 parts of urea, 0.1 to 1 part of a zymophyte and 0.5 to 1 part of dried marine algae powder. The preparation method comprises the steps of material preparation, stacking for fermentation and sterilization, so that the edible mushroom culture medium is prepared. According to the edible mushroom culture medium and the preparation method thereof, the baijiu vinasse and the bagasse are changed into things of value, then the other auxiliary materials are added, so that the edible mushroom culture medium is prepared, and the medium has a good using effect.

The invention discloses a straw type ecological slope protection structure and method. The straw type ecological slope protection structure comprises a hemp fiber layer, a netlike frame formed by straw and planting grooves, wherein the hemp fiber layer is laid on a side slope; the edges, the corners and the middle of the hemp fiber layer are fixed in side slope soil; the netlike frame formed by the straw is arranged on the hemp fiber layer; the positions, at the top and bottom of the slope, of the netlike frame formed by the straw are also fixed in the side slope soil; a plurality of triangular-prism-shaped chambers are formed in the netlike frame; the planting grooves are arranged in the triangular-prism-shaped chambers at intervals; and each of the surfaces of the planting grooves and the netlike frame is also covered with a layer of surface soil.

The invention relates to the field of fruit tree management methods, and particularly discloses an apple sapling field management method. The method comprises first-year management, second-year management, third-year management and fourth-year management. According to the method, through standardized management of samplings, trunk determination, mulching film laying, film bag sleeving, fertilization and the like, the base parts of main branches are free of the phenomenon of bare legs, the germination rate exceeds 60%, and the apple yield is greatly increased.

The invention provides an out-bottle one-step plantlet developing method for rootless test-tube cunninghamia lanceolata seedlings. The out-bottle one-step plantlet developing method includes the steps that by reducing the inoculum density of cunninghamia lanceolata clumpy buds, the illumination intensity of the subculture seedlings in the culturing period is improved, the illumination time of the subculture seedlings in the culturing period is prolonged, the culturing cycle is prolonged, and the subculture seedlings high in lignification degree are obtained; the subculture seedlings are cut, after rooting processing is carried out on the subculture seedlings through rooting agent, the subculture seedlings are transplanted to a sterilized non-woven fabric container cup to be cultured, seedling management is carried out, and cunninghamia lanceolata plantlets are obtained. By means of the out-bottle one-step plantlet developing method, the culturing steps for tissue cultured seedlings are simplified, the links of test-tube seedling in-bottle rooting inducing, seedling hardening and the like are omitted, the rootless test-tube cunninghamia lanceolata seedlings are directly transplanted to nursery gardens for transplanting, the subculture seedlings obtained after subculture enrichment culturing can further develop to be the plantlets, the time and the cost for the steps of test-tube seedling in-bottle rooting, seedling hardening and the like are effectively saved, and the good economic benefits, the good social benefits and the good ecological benefits are achieved.

The invention discloses a propagation method of grating plum blossom using a trigonal scion. The method comprises the steps that (1), a scion and a stock are selected; (2), a trigonal oblique surfaceis cut at the grafting position of the scion; (3), an interface of a trigonal groove is cut at the grafting position of the stock, wherein the shape and size of the interface correspond to the trigonal oblique surface; (4), the scion faces a cutting surface of the stock, a cambium is aligned and inserted into the interface, and the cambium is fastened by using a tie rope; (5), grating is conducted10 days before the germination of the stock in spring at the temperature of 15 DEG C-22 DEG C during the day or at night. According to the method, a trigonal scion is used for grafting, a trigonal groove and a trigonal interface are used by the stock, which has the advantages that (1) the grafting position can easily obtain a long grafting surface; (2), the healing point after grafting is conducted is firmly combined, the growth after grating is more rapid, the fracturing and damaging caused by wind disaster can be effectively prevented, and high grafting survival rate and successful graftingare ensured.

The invention discloses a tissue culture rapid propagation method for illigera trifoliata. The tissue culture rapid propagation method for illigera trifoliate comprises the following steps: (1) taking a stem with a bud of the illigera trifoliate as an explant, inoculating the stem with the bud into a propagation and rejuvenation integrated culture medium, culturing in a culture room for 15 days under the indoor conditions of 22-26 DEG C, 1,400 lux and 8 h/d, and then continuing culture under the natural illumination conditions of 25-28 DEG C and 3,500-4,500 lux for 15 days to obtain cluster buds; (2) inoculating the cluster buds cultured in the step (1) in a rooting and seedling hardening-off synchronization culture medium, culturing the cluster buds for 7 days under the indoor conditions of 22-26 DEG C, 1,500 lux and 10 h/d, and then culturing for 25 days in a greenhouse under the natural light, wherein the temperature is 23-28 DEG C, the light shading degree is 20-40%, and the relative humidity is 60-80%; and (3) transplanting. By the method, the production period of seedlings can be shortened remarkably, the production cost of the seedlings is reduced, the survival rate of the seedlings is increased and the quality of the seedlings is improved.