86results about How to "High degree of lignification" patented technology

The invention discloses a technique for grafting wild Chinese walnut, which comprises: cutting the fracture of a wild walnut or wingnut stock to form an inclined face (1); cutting off 3 to 5 centimeters of skin (2) of the upper end of the higher side of the inclined face (1) to form a 'V' shape (9); cutting off skin (5) in the direction of a bud on a cion branch; peeling the pulp skin layer (3) of the 'V' shape (9) of the stock to separate the pulp skin layer (3) from xylem (4); inserting the inclined plane (6) of a cion from top down while keeping the cion tightly adhering to the xylem (4) of the stock inward; sleeving the whole saw bite on the stock from the joint of the stock and the cion by using an agricultural mulching film; exposing cion branch; and fastening tightly. When the grafting technique is adopted, the operation is simple, the cost is low, and by using wild resources, the grafting survival rate is increased and fruiting is early.

The invention provides a method for planting organic rice. The method comprises the following steps of: planting milk vetch, namely sowing 3.0 to 3.5kg of milk vetch seeds on each mu of land; after the milk vetch enters the initial bloom stage, ploughing and dry-hallowing the land to ensure that the milk vetch is cut into pieces and fully mixed with soil, drying in the sun for 1 to 2 days, and then irrigating water to ret green manure, wherein the retting time is 15 to 20 days; performing land preparation, namely reclaiming ditches to make rectangular land pieces after the land is flattened and the mud is molten; leveling the surfaces of the rectangular land pieces, paving films on the surfaces of the rectangular land pieces, and punching on the surfaces of the rectangular land pieces on which the films are paved after the films are paved for 1 to 3 days; planting rice seedlings in holes of the surfaces of the rectangular land pieces; keeping the rice field in a moistened state, namely keeping water which is lower than the surfaces of the rectangular land pieces in the ditches; and draining away the water and drying the field in the sun 8 to 12 days before the harvest. The method for planting the organic rice has the advantages that: the problem of organic fertilizers can be effectively solved, no weed is grown, plant diseases and insect pests are avoided, planting labor and planting cost are not increased, the high drought-resistant effect is achieved, the management is simple and convenient, the adaptability is strong, and the method is safe and reliable.

The invention relates to a large- scale breeding method of Borneol Cinnamomum camphora. The method comprises the following steps of: breeding a plantlet obtained through unique cuttage treatment by utilizing a current-growth lignified branch of strongly disease-resistant, no pest and disease damage and grown-up seed tree for about one year, acclimatizing, subculturing and propagating a sterile material after obtaining the sterile material with the current germinal sprout as an explant, secondly carrying out strong seeding rootage cultivation, and finally cultivating seeding in a greenhouse. According to the method disclosed by the invention, induced, propagated, strengthened and rooting culture mediums are optimized and improved, the stable growth of the sterile material can be effectively ensured, the sizes of sprouts are uniform, vitrification or defoliation of a tissue culture seedling can be avoided, the strong tissue culture seedlings can be obtained for sure, the repeatability is strong, an acclimation period and a subculture period are stable, a browning situation is reduced, a propagation coefficient is improved, the survival rate of planting in a greenhouse is improved, asubculture block mass cutting method determines the growth and the propagation coefficient of later-stage materials and can meet requirements of large-scale production.

A method for culturing the saplings of pine or juniper by use of containers and plastic hot shed includes such steps as building a plastic hot shed, germinating the seeds of pipe or juniper, perforating on the bottom of earth basin, preparing matrix soil from agricultural field soil, raw clay, organic fertilizer and N,P and K fertilizers, disinfecting the matrix soil, loading in earth basins, sowing, irrigating, covering by a layer of fine sandy soil, spraying mitrofen, and controlling temp and humidity. Its advantages are short breeding time and high germination rate.

The invention discloses a method for rooting and thickening of fructus momordicae tissue culture seedlings and relates to the field of agricultural breeding methods. The method comprises the following steps: induction culture of tissue culture seedlings, multiplication and successive transfer culture of the tissue culture seedlings, thickening and rooting culture of fructus momordicae tissue culture seedlings, hardening-seedling of the thickened and rooted tissue culture seedlings and transplanting for survival. The formula of a thickening and rooting medium adopted by the method has a better synergistic effect, can achieve the purposes of thickening and rooting, meets the transplant requirement and has strong adaptability to the outside environment, thereby improving the transplant survival rate. The tissue culture seedlings obtained by adopting the method are thick and strong, high in disease resistance and lignification degree and can reach the out-planting transplant survival rate of more than 98%.

The present invention relates to the technical field of Luo Han Guo cultivation, in particular to a method for cultivating Luo Han Guo. The branches are collected and sterilized, and the stem apex meristem is cut out for subculture; the seedlings are selected and quickly dipped in IBA, and the lower 1 / 4 of the seedlings are inserted into the Between two pieces of filter paper, clamp the filter paper with two aluminum alloy bedding bars, and fix the aluminum alloy beading bar with a small clip, then hang the aluminum alloy beading bar on the two walls of the rooting incubator for rooting cultivation, and the rooting incubator contains a box volume of 1 / 4 or 1 / 2 MS culture solution; before transplanting, apply quicklime to the planting site, and then apply decomposed farmyard manure, place the seedlings in the planting nest and pour enough water for rooting, after 7 days, wet the fertilizer once a week, When the Luo Han Guo seedling grows more than 3 true leaves, remove the film covered on the Mirs, and apply organic compound fertilizer, the method of the present invention obtains transplanted seedlings, the stems are strong, the rooting rate is high, the quality is good, and the survival rate of field transplantation is high. High and adaptable.

The invention relates to the technical field of plant cultivation substrates, in particular to a high-heat-preservation substrate for recovery of broken hill vegetation in a high elevation region. 45-50% by volume of sand loam, 35-40% by volume of highland barley straw, 3-6% by volume of organic fertilizer, 0.01-0.03% by volume of pellet agent, 0.01-0.03% by volume of polyvinyl acetate, 0.01-0.03%by volume of sodium carboxymethylcellulose, 2-4% by volume of humic acid, 2-4% by volume of liquid emulsified asphalt and 0.03-0.10% by volume of plant seeds are taken, the substrate thickness is 2-6cm, the highland barley straw particle size is 2-4 cm, the organic fertilizer is faeces of Tibetan-sheep and yaks, highland barley straw and rape stalk with the particle size smaller than 4 cm, faeces and the stalk are mixed according to the volume ratio being 1:(1-3) and then subjected to composting fermentation, the composting time is from October to April the following year, turning and ventilation are conducted semimonthly, film mulching is used for covering at night, the fermentation temperature is maintained, and the seeds comprise one or multiple of pennisetum centrasiaticum, orinus thoroldii (Stapf ex Hemsl.) bor, rrtemisia wellbyi Hemsl. et Pears. ex deasy, oxytropis sericopetala, rhododendron nivale and sabina chinensis. The substrate has the advantages of being high in heat preservation, capable of prolonging plant growth time, resistant to washout, good in water-retaining property and capable of increasing the broken hill coverage rate.

The invention discloses a xanthoceras sorbifolia culture method, which relates to the xanthoceras sorbifolia rapid propagation technology and belongs to the technical field of plant tissue culture. The xanthoceras sorbifolia culture method solves the problem of phenomenon of one fruit of thousands of flowers of the xanthoceras sorbifolia. Therefore, the xanthoceras sorbifolia has high seedling differentiation rate, high propagation speed and high seedling yield. The xanthoceras sorbifolia realizes the micropropagation at the speed of several times each month, and the resource regeneration and the utilization of the micropropagation are reached through tender stem or stem tip culture. The xanthoceras sorbifolia culture method has the beneficial effects that the seedling development is healthy and strong, the planting survival rate is high, the seedling raising is early, the growth period is long, the seedling lignification degree is improved, the seedling cold resistance and overwintering capability is enhanced, the seedling raising time is short, the cost is low, and the seedling yield in the unit area is high.

The invention discloses a hard branch patch budding seedling culture method for juglans regia. By means of the technology, the grafting time can be shortened, the working efficiency can be improved, the seedling breeding cycle can be shortened, and the seedling commercialization can be enhanced. The hard branch patch budding seedling culture method for juglans regia includes the first step of scion collection, wherein twigs, with fully mature buds, on the upper half parts of annual branches on the periphery of a crown are collected; the second step of scion treatment and storage, wherein after the scions for grafting are collected, every 30-50 scions are tied with a tag as a bundle and then are stored; the third step of stock selection, wherein robust seedlings with the ground diameter reaching 0.8 cm or above and without plant diseases or insect pests are selected as stocks; the fourth step of grafting, wherein the scions treated in the second step are grafted onto the stocks in the third step according to the grafting method of hard branch patch budding; the fifth step of post-grafting management, wherein the post-grafting management includes supplementary grafting, unwanted sprout removing, stock pruning, untying, fertilizer and water management and pest and disease control.

The invention relates to the technical field of extraction methods of plant effective ingredients and discloses a method for extracting ganoderan from lucid ganoderma sporocarp. The method includes the following steps that lucid ganoderma is crushed, oxidation treatment is conducted through a sodium chlorite-glacial acetic acid system, water is added to residues for ultrasonic extraction, liquid supernatant is subjected to alcohol precipitation and centrifugation, and crude polysaccharide is obtained. According to the method, the sodium chlorite-glacial acetic acid system is utilized for eliminating lignin and cellulose in the lucid ganoderma sporocarp so that more ganoderan in the lucid ganoderma sporocarp can be released; the ganoderan is extracted in organic combination with ultrasound-assisted extraction, and the method is convenient to operate, simple in process, high in yield and suitable for scale production.

The invention discloses a method for preparing lotus tea capable of being brewed with normal-temperature drinking water. According to the method, fresh lotus flowers and fresh loquat leaves are taken as raw materials, picking, spread-drying, fixation, rolling, primary baking, juice adding, drying, spread-drying, weighing and packaging, inspection and storage are conducted on the lotus flowers, the fresh loquat leaves are decocted (extracted) into loquat leaf juice with water, a proper amount of xylitol powder is added to make loquat leaf sweet juice which serves as the juice adding raw material of the lotus tea, and then the lotus tea capable of being brewed with normal-temperature drinking water is prepared. The functional components of the fresh lotus flowers and the fresh loquat leaves are combined, and a small amount of xylitol is used for seasoning, so that the lotus tea has the inherent faint scent of lotus flowers as well as a sweet after taste. The lotus tea can be brewed with normal-temperature drinking water, the traditional idea of brewing tea with high-temperature drinking water is changed, consumption is facilitated for people, and the method accords with the modern consumption view featured in energy saving, environment friendliness and convenience.

The invention discloses a g rafting cultivation method suitable for apple dwarf interstock seedlings in Alpine land. A malus robusta seedling sowed in the first year is selected as a base stock, an SH-line dwarf stock seedling grafted on the base stock is selected as a dwarf interstock seedling, each seedling needs to be full in bud tip, high in lignification degree and with diameter thickness being 0.5-1.2cm, and grafting survival rate is high. A T-shaped bud grafting method is adopted for grafting, and then conventional management like stock cutting, sprout removal, weeding and soil loosening, fertilizing and pest and disease prevention and control is performed on grafted seedlings. Dwarf interstock apple seedlings after successful grafting have obvious dwarf effect. The SH-line dwarf stock is used as an interstock, is highly cold-resistant and can resist low temperature of minus 36 DEG C, thereby being suitable for high-cold high-altitude regions; the dwarf interstock apple seedlings are high in flexibility, windproof, uvioresistant, extremely high in affinity and unobvious in big-small feet phenomena of the base stock and variety seedlings after grafting; fruiting period is advanced, the seedlings can bear fruit in the third year, fruit coloring rate is increased by 25%, and commodity rate is increased by 25%; orchard establishment cost is low.

The invention relates to the technical fields of plant molecular biology and genetic engineering, and provides a pear laccase gene PbLAC1, the nucleotide sequence of which is shown in SEQ ID NO. 1. The invention also provides a plant over-expression vector, wherein the plant over-expression vector comprises pMD-18T-PbLAC1 plasmid constructed by PbLAC1. The invention also provides a genetically engineered host cell comprising a PbLAC1 gene sequence. The invention also provides the application of the pear laccase gene PbLAC1 in regulating plant lignin synthesis and cell wall development. The pear laccase gene PbLAC1 of the present invention provides new evidence for perfecting the plant lignin synthesis pathway, has important theoretical and practical significance for improving fruit qualityand other key agronomic characteristics of crops, and also provides a new way for regulating the content of pear stone cells by genetic engineering.

The invention relates to a method for enabling whole annual Chinese Hongye poplar to resist coldness and overwinter. The method comprises the following steps of: adding 1,000ml of water into abscisic acid and mixing uniformly; spraying the obtained preparation on leaves of the whole Chinese Hongye poplar by using a mist sprayer before the Chinese Hongye poplar hibernates at the beginning of September; spraying the obtained preparation by using the mist sprayer once again after 15 days; and spraying the whole Chinese Hongye poplar in October. By the method, the coldness resistance of the Chinese Hongye poplar can be improved, the phenomenon that all branches of the Chinese Hongye poplar on the ground are frozen to death after the winter is avoided, and the aim of enabling the whole annual Chinese Hongye poplar to overwinter safely is fulfilled.

The invention relates to the technical field of plant growth regulators, and particularly discloses a nursery stock growth promoting and thickening agent. The nursery stock growth promoting and thickening agent is formed by mixing the following raw materials, by weight, 20 percent to 40 percent of chlormequat chloride, 0.1 percent to 0.2 percent of alpha-pimacol, 0.05 percent to 0.1 percent of indolebutyric acid potassium, five percent to 10 percent of monopotassium phosphate and the balance fillers. The nursery stock growth promoting and thickening agent can promote the growth of nursery stocks well, so that the nursery stocks are thick and strong in stem, developed in root system, high in lignification degree and reasonable in seedling height, and the wind resisting capacity and other adversity tolerating capacity of the nursery stocks are improved.

The invention relates to a tree summer implanting method in drought and half-drought regions, wherein phytohormone is utilized to make the nursery stock enter dormant state, the method comprises the steps of, choosing nursery transplant, spraying plant growth hormone medicament, after the nursery-grown plant dormancy, extracting sprouts rapidly through normal procedures and packaging the soil with plastic, irrigating with ferrous sulfate aqueous solution of 0.1% concentration, sprinkling foliage 1-3 times with 5% KH2PO3 solution.

The invention discloses a cultivation method of sound seedlings of blueberry tissue culture seedlings. The cultivation method comprises the following steps: (1) blending a base material; (2) pre-treating bottle seedlings; (3) inserting the seedlings; (4) disinfecting; (5) sealing by a film and culturing; and (6) removing the film and carrying out post-period management. In the step (3), self-made rooting liquid is utilized and cuttage is realized by adopting a manner of avoiding generation of aerial roots; in the step (2) and the step (5), light illumination is realized by adopting an LED (Light Emitting Diode) light source; in the step (6), the post-period management is realized by adopting a specific step and the sound seedlings of the blueberry tissue culture seedlings are produced; and a relatively high survival rate of young blueberry tissue culture seedlings can also be guaranteed when the young blueberry tissue culture seedlings are directly planted into a large field, without the need of a nutrition pot sound seedling heeling-in period. By adopting the cultivation method disclosed by the invention, the seedling cultivation period can be shortened and a middle production link is reduced; and the transportation cost and the seedling cultivation cost are reduced.

The invention discloses a constructing method for a wide-narrow row J.sigillata Dode hard-twig cion cutting orchard. The method comprises the steps of orchard construction location selection, cutting orchard layout and land preparation, field planting of grafted seedlings, management in the same year after field planting and cutting orchard management. An area which has a year-round average temperature of 13-18 DEG C, year-round sunshine duration no smaller than 2,000 h, an annual rainfall of 800-1,200 mm, an elevation of 1,600-2,200 m and a soil pH value of 5.5-7 is selected as an orchard construction location; field planting holes are staggered in two rows, wide row spacing of the grafted seedlings is 200 cm, narrow row spacing of the grafted seedlings is 100 cm, and the soil field moisture capacity of the cutting orchard is no smaller than 60% and no larger than 70%; the field planting is performed in January, and cions can be picked in the same year. Through the method, 6,000-9,000 cions can be yielded per 666.7 square meters in the same year of field planting, 17,000-40,000 cions can be yielded per 666.7 square meters in the second or third year, the cion roughness is 1.0cm-1.5cm, a pith is smaller than 1 / 3 of the cion roughness, cion quality is good, production is rapid, and yield is high.

The invention discloses an edible mushroom culture medium and a preparation method thereof. A formula of the culture medium comprises the following ingredients: 50 to 60 parts of baijiu vinasse, 20 to 30 parts of bagasse, 5 to 15 parts of bran, 1 to 2 parts of plaster stone, 1 to 2 parts of coal, 1 to 2 parts of urea, 0.1 to 1 part of a zymophyte and 0.5 to 1 part of dried marine algae powder. The preparation method comprises the steps of material preparation, stacking for fermentation and sterilization, so that the edible mushroom culture medium is prepared. According to the edible mushroom culture medium and the preparation method thereof, the baijiu vinasse and the bagasse are changed into things of value, then the other auxiliary materials are added, so that the edible mushroom culture medium is prepared, and the medium has a good using effect.

The invention discloses a straw type ecological slope protection structure and method. The straw type ecological slope protection structure comprises a hemp fiber layer, a netlike frame formed by straw and planting grooves, wherein the hemp fiber layer is laid on a side slope; the edges, the corners and the middle of the hemp fiber layer are fixed in side slope soil; the netlike frame formed by the straw is arranged on the hemp fiber layer; the positions, at the top and bottom of the slope, of the netlike frame formed by the straw are also fixed in the side slope soil; a plurality of triangular-prism-shaped chambers are formed in the netlike frame; the planting grooves are arranged in the triangular-prism-shaped chambers at intervals; and each of the surfaces of the planting grooves and the netlike frame is also covered with a layer of surface soil.

The invention provides a method for efficiently cultivating lentinus edodes through mulberry branches. The method specifically includes the steps that mulberry branches cut in summer are selected as raw materials, smashed and aired, and a culture medium is formed by mulberry branch smashed materials, cotton seed hulls, bran and quick lime; then bagging and sterilization, inoculation, fungus cultivation, lentinus edodes producing management and timely harvesting and marketing are conducted. By means of the method, the mulberry branches serve as the raw materials for cultivating lentinus edodes and are made into fungus sticks or fungus bags for lentinus edodes cultivation, each fungus stick (bag) can earn a profit of about 2 yuan, the branches of adult mulberry fields per mu can acquire the income of more than 7,000 yuan when used for cultivating lentinus edodes, the comprehensive utilization value of mulberry field by-products is increased, the sericulture industry chain is lengthened, silkworm breeding and mulberry growing efficacy enhancing ways are expanded, the income of silkworm raisers is fully increased, the profits of the silkworm raisers are maximized, a new way for expanding the raw material source is provided for lentinus edodes cultivation, consumption of natural forests is reduced, the ecological environment is protected, and unification of social benefits, ecological benefits and economic benefits is effectively achieved.

The invention discloses a method for building an early high-yield cutting orchard by macadamia nut grafted seedlings, and belongs to the technical field of reproduction of improved varieties of foresttrees. The method includes the steps of cutting orchard building and management, and cutting tree management and regulation. Particularly, a yellow loam flat land or hilly sloppy land facilitating drainage and irrigation is selected to build the orchard, the mean annual temperature of the land is 19-23 DEG C, the extreme low temperature of the land is higher than or equal to 1.5 DEG C, the annualrainfall of the land is larger than 1100mm, the altitude of the land is smaller than 800m, and the soil layer of the land is larger than 50cm. Grafted seedlings (with the height larger than or equalto 130cm, the sprouting thickness larger than or equal to 1cm, the sprouting length larger than or equal to 70cm and the branching number larger than or equal to 6) of 3-5 local mainly-planted macadamia nut fine varieties are planted, soil, water and fertilizer management and pest control are performed, cutting can be performed in autumn and winter of the year of planting, and management is enhanced to improve production and quality of cuttings after cutting. The macadamia nut cutting orchard is built by the method, and production is early as cutting can be performed in autumn and winter of the year of planting. The cuttings reaching design capacity are improved by 3 times or more as compared with conventional cuttings, and yield is high. The thickness of most cuttings is 0.5-1.0cm, internodes are uniform in density, and the cuttings are high in degree of lignification and excellent in quality.

The invention relates to the field of fruit tree management methods, and particularly discloses an apple sapling field management method. The method comprises first-year management, second-year management, third-year management and fourth-year management. According to the method, through standardized management of samplings, trunk determination, mulching film laying, film bag sleeving, fertilization and the like, the base parts of main branches are free of the phenomenon of bare legs, the germination rate exceeds 60%, and the apple yield is greatly increased.

The invention discloses an arbor type weigela florida thickening and trunk strengthening method. The method comprises the steps of (1) carrying out trunk setting processing of weigela florida seedlings, wherein only the thickest and strongest coppice shoot of each weigela florida seedling is reserved, and pinching and trunk setting are carried out on main branches; (2) pruning the weigela florida seedlings, wherein the sprouts and branches, under 50 cm, of the base portions of main branches are all pruned away in the very year when the trunk setting is carried out on the weigela florida seedlings, pinching processing is carried out on first level branches above 50 cm when the first level branches grow to 1.0 m, cutting-back processing is carried out on the first level branches growing to 1.5 m, and mentioned operation is repeated for twice or three times; in a similar way, carrying out pinching and cutting-back processing on corresponding branches in the second year, the third year and the fourth year respectively, so that thickening and trunk strengthening operation is completed. According to the arbor type weigela florida thickening and trunk strengthening method, the lignification degree of the seedlings is improved, the height-diameter ratio is reasonable, the branch breaking rate of lateral branches can be reduced by more than 95 percent, the lodging rage can be reduced by more than 98 percent, the commodity cycle can be shortened by more than two years, economic effects are improved, and the method also has the advantages that operation is easy, convenient and feasible, low in cost, and the method is easy to apply and popularize.

The invention provides an out-bottle one-step plantlet developing method for rootless test-tube cunninghamia lanceolata seedlings. The out-bottle one-step plantlet developing method includes the steps that by reducing the inoculum density of cunninghamia lanceolata clumpy buds, the illumination intensity of the subculture seedlings in the culturing period is improved, the illumination time of the subculture seedlings in the culturing period is prolonged, the culturing cycle is prolonged, and the subculture seedlings high in lignification degree are obtained; the subculture seedlings are cut, after rooting processing is carried out on the subculture seedlings through rooting agent, the subculture seedlings are transplanted to a sterilized non-woven fabric container cup to be cultured, seedling management is carried out, and cunninghamia lanceolata plantlets are obtained. By means of the out-bottle one-step plantlet developing method, the culturing steps for tissue cultured seedlings are simplified, the links of test-tube seedling in-bottle rooting inducing, seedling hardening and the like are omitted, the rootless test-tube cunninghamia lanceolata seedlings are directly transplanted to nursery gardens for transplanting, the subculture seedlings obtained after subculture enrichment culturing can further develop to be the plantlets, the time and the cost for the steps of test-tube seedling in-bottle rooting, seedling hardening and the like are effectively saved, and the good economic benefits, the good social benefits and the good ecological benefits are achieved.

The invention discloses a propagation method of grating plum blossom using a trigonal scion. The method comprises the steps that (1), a scion and a stock are selected; (2), a trigonal oblique surfaceis cut at the grafting position of the scion; (3), an interface of a trigonal groove is cut at the grafting position of the stock, wherein the shape and size of the interface correspond to the trigonal oblique surface; (4), the scion faces a cutting surface of the stock, a cambium is aligned and inserted into the interface, and the cambium is fastened by using a tie rope; (5), grating is conducted10 days before the germination of the stock in spring at the temperature of 15 DEG C-22 DEG C during the day or at night. According to the method, a trigonal scion is used for grafting, a trigonal groove and a trigonal interface are used by the stock, which has the advantages that (1) the grafting position can easily obtain a long grafting surface; (2), the healing point after grafting is conducted is firmly combined, the growth after grating is more rapid, the fracturing and damaging caused by wind disaster can be effectively prevented, and high grafting survival rate and successful graftingare ensured.

The invention relates to a chitosan mixture and an application thereof in preventing and treating strawberry anthracnose and solves the technical problem that pesticides are used for preventing from diseases at present. The chitosan mixture contains following components in percentage by weight: 10%-30% of chitosan powder, 30%-60% of bamboo charcoal powder and the balance of oyster shell powder. The chitosan mixture can be widely applied to preventing and treating the strawberry anthracnose.

The invention discloses a tissue culture rapid propagation method for illigera trifoliata. The tissue culture rapid propagation method for illigera trifoliate comprises the following steps: (1) taking a stem with a bud of the illigera trifoliate as an explant, inoculating the stem with the bud into a propagation and rejuvenation integrated culture medium, culturing in a culture room for 15 days under the indoor conditions of 22-26 DEG C, 1,400 lux and 8 h / d, and then continuing culture under the natural illumination conditions of 25-28 DEG C and 3,500-4,500 lux for 15 days to obtain cluster buds; (2) inoculating the cluster buds cultured in the step (1) in a rooting and seedling hardening-off synchronization culture medium, culturing the cluster buds for 7 days under the indoor conditions of 22-26 DEG C, 1,500 lux and 10 h / d, and then culturing for 25 days in a greenhouse under the natural light, wherein the temperature is 23-28 DEG C, the light shading degree is 20-40%, and the relative humidity is 60-80%; and (3) transplanting. By the method, the production period of seedlings can be shortened remarkably, the production cost of the seedlings is reduced, the survival rate of the seedlings is increased and the quality of the seedlings is improved.

The invention discloses a quick tissue culture propagation method of ardisia maclurei, which comprises the following steps: A, integrated propagation and rejuvenation culture: taking a stem section with a bud of the ardisia maclurei as an explant, inoculating the explant into the integrated propagation and rejuvenation culture medium, transferring into a culture room for culture after inoculation is finished and obtaining a robust cluster bud, B, synchronous rootage and seedling exercising culture: inoculating the cluster bud obtained in step A into a synchronous rootage and seedling exercising culture medium, transferring into the culture room for culture after the inoculation is finished, then transferring into a seedling raising greenhouse for culture and obtaining a complete plant appropriate for transplanting, and C, transplanting: taking out the complete plant obtained in step B, clearing away the residual culture medium at a root, and transplanting to a seedling raising bed or the seedling raising greenhouse to continue the culture. The method has the advantages of short production period, low production cost, good seedling quality, high survival rate and the like and can provide technical support for variety protection and artificial propagation of the ardisia maclurei.