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Cultivation method of sound seedlings of blueberry tissue culture seedlings

A cultivation method and technology for tissue culture seedlings, applied in the field of plant biology, can solve the problems of high cost of nutrient pot planting, increased production cost of seedlings, shortened field growth period, etc. The effect of increasing the degree of lignification

Inactive Publication Date: 2017-05-10
HEFEI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to improve the survival rate of transplanted tissue cultured seedlings, according to the current industry situation, it usually takes at least one year for blueberry tissue cultured seedlings to be transplanted in a single plant nutrient pot during the period from rooting outside the bottle in the cultivation room to transplanting in the field. The intermediate link of planting requires a lot of manpower and material resources, which increases the production cost of seedlings. Not only that, the growth period of the field is correspondingly shortened, thus delaying the breeding and promotion of excellent seedlings
Moreover, there are problems such as high cost of planting in nutrient pots, complicated procedures, and weak root system in the later stage of planting.

Method used

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  • Cultivation method of sound seedlings of blueberry tissue culture seedlings

Examples

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Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: This example is a method for cultivating strong seedlings of tissue culture seedlings of Nangao blueberry variety O'Neill. The steps are:

[0026] (1) Substrate preparation: soak the dried moss in water with a pH of 4.5 for 12 hours and then put it into a cleaned and disinfected nursery pot. The amount of moss filled is one-third of the volume of the nursery pot, and then evenly spray and disinfect The disinfection water contains 0.2 g of carbendazim and 0.2 g of phytophthora per liter of water for 1 to 2 times, and the rest is water with a pH of 4.5. The spraying amount of the disinfected water is 5-7 ml / time / pot.

[0027] (2) Pretreatment of bottle seedlings: Put the blueberry tissue culture bottle seedlings under the LED light source (red and blue spectrum ratio is 8:1) and irradiate them for one week. Use scissors at a 45° angle, 0.5~1cm above the medium. Cut blueberry tissue culture seedlings at the position, each with a length of 2 to 3 cm, remove the two le...

Embodiment 2

[0032] Example 2: This example is a method for cultivating strong seedlings of tissue culture seedlings of Nangao blueberry variety Mist. The steps are:

[0033] (1) Substrate preparation: soak the dried moss in water with a pH of 5.0 for 13 hours and then put it into a cleaned and disinfected nursery pot. The amount of moss is one-third of the volume of the nursery pot, and then evenly spray and disinfect The disinfection water contains 0.22 g of carbendazim and 0.22 g of Phytophthora per liter of water for 1 to 2 times, and the rest is water with a pH of 5.0. The spray amount of the disinfectant water is 5-7 ml / time / pot.

[0034] (2) Pretreatment of bottle seedlings: Put the blueberry tissue culture bottle seedlings under the LED light source (red and blue spectrum ratio is 7:1) and irradiate them for one week, then use scissors to make a 40° angle, 0.5~1cm above the medium. Cut blueberry tissue culture seedlings at the position, each with a length of 2 to 3 cm, remove the two le...

Embodiment 3

[0039] Example 3: This example is a method for cultivating strong seedlings of tissue culture seedlings of brilliant rabbit-eye blueberry varieties. The steps are:

[0040] (1) Substrate preparation: soak the dried moss in water with a pH of 5.5 for 15 hours and then put it into a cleaned and disinfected nursery pot. The amount of moss filled is one-third of the volume of the nursery pot, and then evenly spray and disinfect The disinfection water contains 0.25 g of carbendazim and 0.25 g of Phytophthora per liter of water for 1 to 2 times, and the rest is water with a pH of 5.5, and the spraying amount of the disinfection water is 5-7 ml / time / pot.

[0041] (2) Pretreatment of bottle seedlings: Put the blueberry tissue culture bottle seedlings under the LED light source (red and blue spectrum ratio is 7:1) and irradiate them for a week, and use scissors at a 50° angle, 0.5-1cm above the medium Cut blueberry tissue culture seedlings at the position, each with a length of 2 to 3 cm, r...

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Abstract

The invention discloses a cultivation method of sound seedlings of blueberry tissue culture seedlings. The cultivation method comprises the following steps: (1) blending a base material; (2) pre-treating bottle seedlings; (3) inserting the seedlings; (4) disinfecting; (5) sealing by a film and culturing; and (6) removing the film and carrying out post-period management. In the step (3), self-made rooting liquid is utilized and cuttage is realized by adopting a manner of avoiding generation of aerial roots; in the step (2) and the step (5), light illumination is realized by adopting an LED (Light Emitting Diode) light source; in the step (6), the post-period management is realized by adopting a specific step and the sound seedlings of the blueberry tissue culture seedlings are produced; and a relatively high survival rate of young blueberry tissue culture seedlings can also be guaranteed when the young blueberry tissue culture seedlings are directly planted into a large field, without the need of a nutrition pot sound seedling heeling-in period. By adopting the cultivation method disclosed by the invention, the seedling cultivation period can be shortened and a middle production link is reduced; and the transportation cost and the seedling cultivation cost are reduced.

Description

Technical field [0001] The invention belongs to the field of plant biotechnology, and specifically relates to a method for cultivating strong seedlings of blueberry tissue culture seedlings. Background technique [0002] Blueberry (Vaccinium Spp), also known as bilberry and bilberry, belongs to the vaccinium plant of the Ericaceae family (Ericaceae), and is a small berry of a perennial shrub. The blueberry fruit not only has high nutritional value, but also has important health care and medicinal value. Especially, the fruit is rich in anthocyanins, which can prevent thrombosis and arteriosclerosis, fight cancer, resist urinary system infection, prevent heart disease and delay Aging has a very good effect. It is known as "21st century functional health berry" and "Queen of fruits", and it has been listed as one of the five health foods for human beings by the International Food and Agriculture Organization. It has become the main newly cultivated fruit in the world today. One of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01G7/04A01G1/00
CPCA01G7/045A01G22/00A01G24/00A01G31/00Y02P60/14
Inventor 徐德聪王子迎沈章军付欢欢吴林生
Owner HEFEI NORMAL UNIV
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