Quick tissue culture propagation method of ardisia maclurei

Abstract

The invention discloses a quick tissue culture propagation method of ardisia maclurei, which comprises the following steps: A, integrated propagation and rejuvenation culture: taking a stem section with a bud of the ardisia maclurei as an explant, inoculating the explant into the integrated propagation and rejuvenation culture medium, transferring into a culture room for culture after inoculation is finished and obtaining a robust cluster bud, B, synchronous rootage and seedling exercising culture: inoculating the cluster bud obtained in step A into a synchronous rootage and seedling exercising culture medium, transferring into the culture room for culture after the inoculation is finished, then transferring into a seedling raising greenhouse for culture and obtaining a complete plant appropriate for transplanting, and C, transplanting: taking out the complete plant obtained in step B, clearing away the residual culture medium at a root, and transplanting to a seedling raising bed or the seedling raising greenhouse to continue the culture. The method has the advantages of short production period, low production cost, good seedling quality, high survival rate and the like and can provide technical support for variety protection and artificial propagation of the ardisia maclurei.

Description

technical field

[0001] The invention relates to the field of plant tissue culture. More specifically, the present invention relates to a tissue culture rapid propagation method of Zijinniu xinye. Background technique

[0002] Zijinniu with heart leaves, also known as Zoumafeng, Hongyuncao, and red carpet, is a plant of the genus Zijinniu in the family Zijinuidae, and it is the Zoumafeng in the "Seventy-two Winds" of Yao medicine. Zijinniu heart leaf is a commonly used medicinal material of Zhuang and Yao people in Guangxi. The whole plant is used as medicine. It is cool in nature and bitter in taste. It has the effects of clearing away heat and detoxifying, antitussive and expectorant, promoting blood circulation and stopping bleeding, regulating meridian and collaterals, reducing swelling and relieving pain. It is used to treat hematemesis , blood in the stool, hemoptysis, metrorrhagia, boils, tuberculosis, chronic bronchitis, cough and asthma, irregular menstruation, dysm...

Examples

Embodiment 1

[0027] A tissue culture rapid propagation method of the heart leaf purple golden cattle, comprising the steps of:

[0028] A. Integrated culture of reproduction and rejuvenation: after disinfection, the stem section of the heart leaf Zijinniu with axillary buds is inoculated into the propagation medium of MS+1.5mg / LBA+0.3mg / L LFS+0.5mg / L IBA to cultivate adventitious buds. Cut the adventitious buds into 1.0 cm budded stem segments, spread the budded stem segments on the surface of the integrated culture medium for reproduction and rejuvenation, and press 1 / 2 of the budded stem segments into the integrated culture for propagation and rejuvenation After the inoculation, it was transferred to the culture room, and cultivated for 15 days under the conditions of culture temperature 22°C, light intensity 1400lux, and light time 8h / d, and then under natural light conditions of culture temperature 25°C and light intensity 3500lux. Continue to cultivate for 15 days to obtain robust clu...

Embodiment 2

[0033] A tissue culture rapid propagation method of the heart leaf purple golden cattle, comprising the steps of:

[0034] A. Integrated culture of reproduction and rejuvenation: after disinfection, the stem section of the heart leaf Zijinniu with axillary buds is inoculated into the propagation medium of MS+1.5mg / LBA+0.3mg / L LFS+0.5mg / L IBA to cultivate adventitious buds. Cut the adventitious buds into 1.5cm budded stem segments, spread the budded stem segments on the surface of the integrated culture medium for reproduction and rejuvenation, and press 1 / 2 of the budded stem segments into the integrated culture for propagation and rejuvenation After the inoculation, it was transferred to the culture room, and cultivated for 15 days under the conditions of culture temperature 24°C, light intensity 1400lux, and light time 8h / d, and then under natural light conditions of culture temperature 26°C and light intensity 4000lux. Continue to cultivate for 15 days to obtain robust clus...

Embodiment 3

[0039] A tissue culture rapid propagation method of the heart leaf purple golden cattle, comprising the steps of:

[0040] A. Integrated culture of reproduction and rejuvenation: after disinfection, the stem section of the heart leaf Zijinniu with axillary buds is inoculated into the propagation medium of MS+1.5mg / LBA+0.3mg / L LFS+0.5mg / L IBA to cultivate adventitious buds. Cut the adventitious buds into 2.0 cm budded stem segments, spread the budded stem segments on the surface of the integrated culture medium for reproduction and rejuvenation, and press 1 / 2 of the budded stem segments into the integrated culture for propagation and rejuvenation After the inoculation, it was transferred to the culture room, and cultivated for 15 days under the conditions of culture temperature 26°C, light intensity 1400lux, and light time 8h / d, and then under natural light conditions of culture temperature 28°C and light intensity 4500lux. Continue to cultivate for 15 days to obtain robust clu...

technical field

[0001] The invention relates to the field of plant tissue culture. More specifically, the present invention relates to a tissue culture rapid propagation method of Zijinniu xinye. Background technique

[0002] Zijinniu with heart leaves, also known as Zoumafeng, Hongyuncao, and red carpet, is a plant of the genus Zijinniu in the family Zijinuidae, and it is the Zoumafeng in the "Seventy-two Winds" of Yao medicine. Zijinniu heart leaf is a commonly used medicinal material of Zhuang and Yao people in Guangxi. The whole plant is used as medicine. It is cool in nature and bitter in taste. It has the effects of clearing away heat and detoxifying, antitussive and expectorant, promoting blood circulation and stopping bleeding, regulating meridian and collaterals, reducing swelling and relieving pain. It is used to treat hematemesis , blood in the stool, hemoptysis, metrorrhagia, boils, tuberculosis, chronic bronchitis, cough and asthma, irregular menstruation, dysm...

Examples

Embodiment 1

[0027] A tissue culture rapid propagation method of the heart leaf purple golden cattle, comprising the steps of:

[0028] A. Integrated culture of reproduction and rejuvenation: after disinfection, the stem section of the heart leaf Zijinniu with axillary buds is inoculated into the propagation medium of MS+1.5mg / LBA+0.3mg / L LFS+0.5mg / L IBA to cultivate adventitious buds. Cut the adventitious buds into 1.0 cm budded stem segments, spread the budded stem segments on the surface of the integrated culture medium for reproduction and rejuvenation, and press 1 / 2 of the budded stem segments into the integrated culture for propagation and rejuvenation After the inoculation, it was transferred to the culture room, and cultivated for 15 days under the conditions of culture temperature 22°C, light intensity 1400lux, and light time 8h / d, and then under natural light conditions of culture temperature 25°C and light intensity 3500lux. Continue to cultivate for 15 days to obtain robust clu...

Embodiment 2

[0033] A tissue culture rapid propagation method of the heart leaf purple golden cattle, comprising the steps of:

[0034] A. Integrated culture of reproduction and rejuvenation: after disinfection, the stem section of the heart leaf Zijinniu with axillary buds is inoculated into the propagation medium of MS+1.5mg / LBA+0.3mg / L LFS+0.5mg / L IBA to cultivate adventitious buds. Cut the adventitious buds into 1.5cm budded stem segments, spread the budded stem segments on the surface of the integrated culture medium for reproduction and rejuvenation, and press 1 / 2 of the budded stem segments into the integrated culture for propagation and rejuvenation After the inoculation, it was transferred to the culture room, and cultivated for 15 days under the conditions of culture temperature 24°C, light intensity 1400lux, and light time 8h / d, and then under natural light conditions of culture temperature 26°C and light intensity 4000lux. Continue to cultivate for 15 days to obtain robust clus...

Embodiment 3

[0039] A tissue culture rapid propagation method of the heart leaf purple golden cattle, comprising the steps of:

[0040] A. Integrated culture of reproduction and rejuvenation: after disinfection, the stem section of the heart leaf Zijinniu with axillary buds is inoculated into the propagation medium of MS+1.5mg / LBA+0.3mg / L LFS+0.5mg / L IBA to cultivate adventitious buds. Cut the adventitious buds into 2.0 cm budded stem segments, spread the budded stem segments on the surface of the integrated culture medium for reproduction and rejuvenation, and press 1 / 2 of the budded stem segments into the integrated culture for propagation and rejuvenation After the inoculation, it was transferred to the culture room, and cultivated for 15 days under the conditions of culture temperature 26°C, light intensity 1400lux, and light time 8h / d, and then under natural light conditions of culture temperature 28°C and light intensity 4500lux. Continue to cultivate for 15 days to obtain robust clu...