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Method for extracting ganoderan from lucid ganoderma sporocarp

A technology of Ganoderma lucidum fruiting bodies and Ganoderma polysaccharides, applied in the field of extracting Ganoderma lucidum polysaccharides from Ganoderma lucidum fruiting bodies, which can solve the problems of environmental pollution, high degree of lignification, high temperature, etc., and achieve the effects of improving extraction efficiency, simple operation, and mild conditions

Active Publication Date: 2016-02-03
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional hot water extraction method has the problems of long extraction time, high temperature, and low yield. extraction techniques and methods, but the extraction effect of these methods is not ideal
Different from other edible fungi, the fruit body of Ganoderma lucidum has a high degree of lignification and complex structure. Polysaccharides are generally combined with cellulose and lignin through physical or chemical bonds. It is difficult to extract polysaccharides by general methods.
As can be seen from the current research results, the extraction rate of water-soluble Ganoderma lucidum polysaccharides is only about 3%, and most of the remaining polysaccharides are retained in the residue. If they are directly discarded, resources will be wasted and the environment will be damaged. create pollution

Method used

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  • Method for extracting ganoderan from lucid ganoderma sporocarp
  • Method for extracting ganoderan from lucid ganoderma sporocarp
  • Method for extracting ganoderan from lucid ganoderma sporocarp

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Experimental program
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Effect test

Embodiment 1

[0028] The method for extracting Ganoderma lucidum polysaccharide from Ganoderma lucidum fruiting body, such as figure 1 shown, including the following steps,

[0029] A. Raw material pretreatment: after drying and crushing the fruiting bodies of Ganoderma lucidum, mix the crushed fruiting bodies of Ganoderma lucidum with water to form a mixed solution, and the weight ratio of fruiting bodies of Ganoderma lucidum to water is 1:20;

[0030] B. Sodium chlorite-glacial acetic acid treatment: put the mixed solution in a water bath at 60°C, add sodium chlorite accounting for 40% of the dry weight of Ganoderma lucidum, and after stirring, add 40% of the dry weight of Ganoderma lucidum dropwise every 1 hour glacial acetic acid, stir after dripping glacial acetic acid, drip glacial acetic acid three times according to the above method, and stir after each dripping glacial acetic acid;

[0031] Put it in a shaker and oscillate, then centrifuge to obtain supernatant I and precipitate I...

Embodiment 2

[0040] The method for extracting Ganoderma lucidum polysaccharide from Ganoderma lucidum fruiting body, such as figure 1 shown, including the following steps,

[0041] A. Raw material pretreatment: after drying and crushing the fruiting bodies of Ganoderma lucidum, mixing the crushed fruiting bodies of Ganoderma lucidum with water to form a mixed solution, the weight ratio of fruiting bodies of Ganoderma lucidum to water is 1:30;

[0042] B. Sodium chlorite-glacial acetic acid treatment: put the mixed solution in a water bath at 80°C, add sodium chlorite accounting for 40% of the dry weight of Ganoderma lucidum, and after stirring, add 60% of the dry weight of Ganoderma lucidum dropwise every 1 hour glacial acetic acid, stir after dripping glacial acetic acid, drip glacial acetic acid three times according to the above method, and stir after each dripping glacial acetic acid;

[0043] Put it in a shaker and oscillate, then centrifuge to obtain supernatant I and precipitate I;...

Embodiment 3

[0051] The method for extracting Ganoderma lucidum polysaccharide from Ganoderma lucidum fruiting body, such as figure 1 shown, including the following steps,

[0052] A. Raw material pretreatment: after drying and crushing the fruiting bodies of Ganoderma lucidum, mix the crushed fruiting bodies of Ganoderma lucidum with water to form a mixed solution, and the weight ratio of fruiting bodies of Ganoderma lucidum to water is 1:25;

[0053] B. Sodium chlorite-glacial acetic acid treatment: put the mixed solution in a water bath at 65°C, add sodium chlorite accounting for 40% of the dry weight of Ganoderma lucidum, and after stirring, add 45% of the dry weight of Ganoderma lucidum dropwise every 1 hour glacial acetic acid, stir after dripping glacial acetic acid, drip glacial acetic acid three times according to the above method, and stir after each dripping glacial acetic acid;

[0054] Put it in a shaker and oscillate, then centrifuge to obtain supernatant I and precipitate I...

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Abstract

The invention relates to the technical field of extraction methods of plant effective ingredients and discloses a method for extracting ganoderan from lucid ganoderma sporocarp. The method includes the following steps that lucid ganoderma is crushed, oxidation treatment is conducted through a sodium chlorite-glacial acetic acid system, water is added to residues for ultrasonic extraction, liquid supernatant is subjected to alcohol precipitation and centrifugation, and crude polysaccharide is obtained. According to the method, the sodium chlorite-glacial acetic acid system is utilized for eliminating lignin and cellulose in the lucid ganoderma sporocarp so that more ganoderan in the lucid ganoderma sporocarp can be released; the ganoderan is extracted in organic combination with ultrasound-assisted extraction, and the method is convenient to operate, simple in process, high in yield and suitable for scale production.

Description

technical field [0001] The invention relates to the technical field of plant active ingredient extraction methods, in particular to a method for extracting ganoderma polysaccharides from ganoderma lucidum fruiting bodies. Background technique [0002] Ganoderma lucidum is the general term for red lucidum and purple lucidum, also known as "Ganoderma lucidum" and "Fairy Grass". It was first recorded in "Shen Nong's Herbal Classic". If the fungus is eaten directly, it generally cannot achieve the therapeutic effect. Usually, its active ingredients can only be extracted, separated and purified to improve the efficacy and exert certain pharmacological effects. In the preparation process of active substances, extraction is the first step and the most critical step. How to release the active ingredients to the greatest extent is of great significance. Hot water extraction is one of the commonly used methods to extract Ganoderma lucidum polysaccharides. However, the traditional ho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 张作法蔡为明吕国英金群力范雷法苗立祥
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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