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Xanthoceras sorbifolia culture method

A method for cultivating and cultivating a crown fruit, which can be applied in horticultural methods, botanical equipment and methods, and gardening, etc., can solve the problems such as the reduction of variety resources and the shrinking of the crown fruit, and achieve short seedling time, long growth period, and robust development. Effect

Inactive Publication Date: 2012-04-18
魏波
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AI Technical Summary

Problems solved by technology

However, due to the lack of investment in the protection of X. sorbifolium forests, the variety resource forests of X. sorbifolia are shrinking day by day, and the variety resources are seriously reduced.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Select the tender stems and shoot tips of Xanthos sorbifolium, wash them twice with water, wash and disinfect them twice with 70% ethanol, wash them twice with sterile water, cut them into 1cm stem segments, and carry out callus induction and differentiation;

[0019] 2. Disinfect the above-mentioned explants and inoculate them on MS+2, 4-D 1.0mg / L medium and cultivate them for 30 days. Wounded tissue; after being differentiated from the callus, it was transplanted to MS+6-BA 4mg / L+IBM 0.2mg / L+GA6mg / L+sucrose 2.2% medium, and the tufted seedlings were differentiated into tufted bud;

[0020] 3. Proliferation of adventitious buds and induction of adventitious roots: The cultured adventitious buds were individually cut and transferred to the medium for proliferation and culture, and the seedlings that formed proliferating bodies were transferred to the medium of 1 / 2MS+IBA 0.2mg / L, and cultured to grow Seedlings with a height of 4cm were cut individually and transferre...

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Abstract

The invention discloses a xanthoceras sorbifolia culture method, which relates to the xanthoceras sorbifolia rapid propagation technology and belongs to the technical field of plant tissue culture. The xanthoceras sorbifolia culture method solves the problem of phenomenon of one fruit of thousands of flowers of the xanthoceras sorbifolia. Therefore, the xanthoceras sorbifolia has high seedling differentiation rate, high propagation speed and high seedling yield. The xanthoceras sorbifolia realizes the micropropagation at the speed of several times each month, and the resource regeneration and the utilization of the micropropagation are reached through tender stem or stem tip culture. The xanthoceras sorbifolia culture method has the beneficial effects that the seedling development is healthy and strong, the planting survival rate is high, the seedling raising is early, the growth period is long, the seedling lignification degree is improved, the seedling cold resistance and overwintering capability is enhanced, the seedling raising time is short, the cost is low, and the seedling yield in the unit area is high.

Description

technical field [0001] The invention relates to rapid propagation technology of sorbifolia, belonging to the technical field of plant tissue culture, in particular to a method for cultivating radix sorbifolium. Background technique [0002] Xanthoceras sorbifolia Bunge. Also known as Papaya, Wendengge, and Sengdeng Maodao, it belongs to Sapindaceae and is a single species; it has high comprehensive utilization value, strong adaptability and stress resistance ability. It can grow everywhere except in saline-alkali land or waterlogged depressions. It is a pioneer tree species for greening barren hills; it has a well-developed root system and strong root germination, and it is an excellent tree species for water and soil conservation and sand prevention and improvement of the environment; the oil content of X. sorbifolium seeds is 300 %, the oil content of the seed kernel is as high as 60%. It is a kind of woody oil plant unique to China, known as the northern Camellia oleifer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 魏波
Owner 魏波
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