Method for cultivating fructus momordicae
A cultivation method and technique of Luo Han Guo, which are applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of Luo Han Guo tissue culture seedlings being slender, brittle and easy to break, requiring light intensity, and low planting survival rate, etc. Effects of medicines and electricity, improving rooting rate and quality, and improving nutritional structure
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Embodiment 1
[0032] (1) Seed selection: select varieties with robust growth, timely blooming, easy fruiting, large and tidy fruit, and collection of branches on a single plant;
[0033] (2) Subculture: soak the shoots in a carbendazim solution with a mass fraction of 0.1% for 15 minutes, cut the shoot apex meristem, and then inoculate it into a subculture medium at a temperature of 23°C and light intensity. 1700Lx, subculture for 45d under the condition of 8h / d of light;
[0034] (3) Induce rooting: select the single bud stem segment of the subculture tissue culture seedling as the seedling for rooting culture, quickly dip the seedling in IBA with a concentration of 1000 mg / L, and immediately insert the lower 1 / 4 of the seedling between two pieces of filter paper. In between, clamp the filter paper with two aluminum alloy strips, and fix the aluminum alloy strips with small clips, and then hang the aluminum alloy strips on the two walls of the rooting incubator, which contains 1 / 4 of the M...
Embodiment 2
[0045] (1) Seed selection: select varieties with robust growth, timely blooming, easy fruiting, large and tidy fruit, and collection of branches on a single plant;
[0046] (2) Subculture: After soaking the branches in a carbendazim solution with a mass fraction of 0.1% for 20 minutes, the shoot apex meristem was cut, and then inoculated into the subculture medium at a temperature of 24°C and a light intensity of 24°C. 2300Lx, subculture for 46d under the condition of 5h / d light;
[0047] (3) Induce rooting: select the single bud stem segment of the subculture tissue culture seedling as the seedling for rooting culture, quickly dip the seedling in IBA with a concentration of 1000 mg / L, and immediately insert the lower 1 / 4 of the seedling between two pieces of filter paper. In between, clamp the filter paper with two aluminum alloy strips, and fix the aluminum alloy strips with small clips, and then hang the aluminum alloy strips on the two walls of the rooting incubator, which c...
Embodiment 3
[0058] (1) Seed selection: select varieties with robust growth, timely blooming, easy fruiting, large and tidy fruit, and collection of branches on a single plant;
[0059] (2) Subculture: After soaking the shoots in a carbendazim solution with a mass fraction of 0.1% for 10 minutes, cut the shoot apex meristem, and then inoculate it into the subculture medium at a temperature of 25°C and a light intensity of 25°C. 1500Lx, subculture for 48d under the condition of 7h / d of light;
[0060] (3) Induce rooting: select the single bud stem segment of the subculture tissue culture seedling as the seedling for rooting culture, quickly dip the seedling in IBA with a concentration of 1000 mg / L, and immediately insert the lower 1 / 4 of the seedling between two pieces of filter paper. During the time, clamp the filter paper with two aluminum alloy strips, and fix the aluminum alloy strips with small clips, and then hang the aluminum alloy strips on the two walls of the rooting incubator, w...
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