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Method for cultivating fructus momordicae

A cultivation method and technique of Luo Han Guo, which are applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of Luo Han Guo tissue culture seedlings being slender, brittle and easy to break, requiring light intensity, and low planting survival rate, etc. Effects of medicines and electricity, improving rooting rate and quality, and improving nutritional structure

Inactive Publication Date: 2016-10-26
GUIZHOU DEJIANG YISHENG AGRI TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although Momordica grosvenori is used as a material with high nutritional value for both medicine and food, its cultivation technology has the following disadvantages: 1. Because the tissue cultured seedlings of grosvenoria grosvenori are slender, brittle and easy to break, and are prone to dehydration and wilting, the survival rate of colonization is low 1. The rate of flowering and fruiting is low, which causes economic losses and increases the risk of planting; 2. Judging from the current status of the rapid propagation technology of Luo Han Guo tissue culture seedlings for rooting, most of them use sugars in the medium as energy sources. Cultivation in a closed and sterile environment with constant light intensity, a wide variety of drugs used in the culture medium and high prices, the cultivation process requires fine equipment and light intensity, which in turn consumes a lot of electricity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Seed selection: select varieties with robust growth, timely blooming, easy fruiting, large and tidy fruit, and collection of branches on a single plant;

[0033] (2) Subculture: soak the shoots in a carbendazim solution with a mass fraction of 0.1% for 15 minutes, cut the shoot apex meristem, and then inoculate it into a subculture medium at a temperature of 23°C and light intensity. 1700Lx, subculture for 45d under the condition of 8h / d of light;

[0034] (3) Induce rooting: select the single bud stem segment of the subculture tissue culture seedling as the seedling for rooting culture, quickly dip the seedling in IBA with a concentration of 1000 mg / L, and immediately insert the lower 1 / 4 of the seedling between two pieces of filter paper. In between, clamp the filter paper with two aluminum alloy strips, and fix the aluminum alloy strips with small clips, and then hang the aluminum alloy strips on the two walls of the rooting incubator, which contains 1 / 4 of the M...

Embodiment 2

[0045] (1) Seed selection: select varieties with robust growth, timely blooming, easy fruiting, large and tidy fruit, and collection of branches on a single plant;

[0046] (2) Subculture: After soaking the branches in a carbendazim solution with a mass fraction of 0.1% for 20 minutes, the shoot apex meristem was cut, and then inoculated into the subculture medium at a temperature of 24°C and a light intensity of 24°C. 2300Lx, subculture for 46d under the condition of 5h / d light;

[0047] (3) Induce rooting: select the single bud stem segment of the subculture tissue culture seedling as the seedling for rooting culture, quickly dip the seedling in IBA with a concentration of 1000 mg / L, and immediately insert the lower 1 / 4 of the seedling between two pieces of filter paper. In between, clamp the filter paper with two aluminum alloy strips, and fix the aluminum alloy strips with small clips, and then hang the aluminum alloy strips on the two walls of the rooting incubator, which c...

Embodiment 3

[0058] (1) Seed selection: select varieties with robust growth, timely blooming, easy fruiting, large and tidy fruit, and collection of branches on a single plant;

[0059] (2) Subculture: After soaking the shoots in a carbendazim solution with a mass fraction of 0.1% for 10 minutes, cut the shoot apex meristem, and then inoculate it into the subculture medium at a temperature of 25°C and a light intensity of 25°C. 1500Lx, subculture for 48d under the condition of 7h / d of light;

[0060] (3) Induce rooting: select the single bud stem segment of the subculture tissue culture seedling as the seedling for rooting culture, quickly dip the seedling in IBA with a concentration of 1000 mg / L, and immediately insert the lower 1 / 4 of the seedling between two pieces of filter paper. During the time, clamp the filter paper with two aluminum alloy strips, and fix the aluminum alloy strips with small clips, and then hang the aluminum alloy strips on the two walls of the rooting incubator, w...

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PUM

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Abstract

The present invention relates to the technical field of Luo Han Guo cultivation, in particular to a method for cultivating Luo Han Guo. The branches are collected and sterilized, and the stem apex meristem is cut out for subculture; the seedlings are selected and quickly dipped in IBA, and the lower 1 / 4 of the seedlings are inserted into the Between two pieces of filter paper, clamp the filter paper with two aluminum alloy bedding bars, and fix the aluminum alloy beading bar with a small clip, then hang the aluminum alloy beading bar on the two walls of the rooting incubator for rooting cultivation, and the rooting incubator contains a box volume of 1 / 4 or 1 / 2 MS culture solution; before transplanting, apply quicklime to the planting site, and then apply decomposed farmyard manure, place the seedlings in the planting nest and pour enough water for rooting, after 7 days, wet the fertilizer once a week, When the Luo Han Guo seedling grows more than 3 true leaves, remove the film covered on the Mirs, and apply organic compound fertilizer, the method of the present invention obtains transplanted seedlings, the stems are strong, the rooting rate is high, the quality is good, and the survival rate of field transplantation is high. High and adaptable.

Description

technical field [0001] The invention relates to the technical field of cultivation of Luo Han Guo, in particular to a cultivation method of Luo Han Guo. Background technique [0002] Luo Han Guo is the fruit of the perennial vines of the genus Luo Han Guo in the Cucurbitaceae family. It is unique to my country and is known as a good medicine and a good fruit. The fruit is rich in glucose, fructose and various vitamins, etc. It has a wide range of uses and sells well in domestic and foreign markets, enjoying a high reputation in the international market. [0003] As a precious medicinal material, Luo Han Guo is cool in nature and sweet in taste, and has functions of clearing the lungs and moistening the intestines. Indications for whooping cough, phlegm-fire cough, blood-dry constipation, etc. It has a good curative effect on the treatment of acute bronchitis, acute tonsillitis, throat fire, and acute gastritis. Use its roots to mash and apply it to the affected area to cure...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张俊连罗小廷张俊蛟
Owner GUIZHOU DEJIANG YISHENG AGRI TECH DEV CO LTD
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