The invention relates to an injection for clearing heat, eliminating phlegm and removing toxicity. The injection comprises an active material, propylene glycol and injection water. The active material is prepared from bear gall powder or ursodesoxycholic acid, cornu gorais, scutellaria baicalensis, honeysuckle and fructus forsythia. The invention further relates to a preparing method and pharmaceutical application of the injection. The injection can be used for clearing heat, eliminating phlegm and removing toxicity, can be used for treating the wind-warm lung-heat disease and the syndrome of phlegm-heat obstructing lung, can be used for treating fever, coughing, expectoration, swollen sore throat, thirsty, reddened tongue and yellow tongue coating, and can also be used for treating the early stage of pneumonia, acute bronchitis, chronic bronchitis acute attack and upper respiratory infection with the mentioned syndromes.