The invention belongs to the technical field of nutritional soil, and particularly relates to medium and trace element mineral nutritional soil. The medium and trace element mineral nutritional soil is mainly prepared from attapulgite, humic acid, perlite, kieselguhr, kaolin, zeolite, vermiculite, sodium metaphosphate and plant ash. The preparation method comprises the following steps that S1, theattapulgite, the perlite, the kaolin, the zeolite and the vermiculite are crushed to be 100-200 mesh separately; S2, the attapulgite is subjected to alkali treatment; S3, the attapulgite subjected toalkali treatment is put into a reaction kettle, heat treatment is conducted, and modified attapulgite is obtained; S4, the vermiculite is put into the reaction kettle, heat treatment is conducted, and modified vermiculite is obtained; S5, the crushed perlite, kaolin and zeolite and the modified attapulgite, the modified vermiculite, the humic acid, the kieselguhr, the sodium metaphosphate and theplant ash are weighed and mixed to be uniform, and the medium and trace element mineral nutritional soil is obtained. Accordingly, the medium and trace element mineral nutritional soil has the advantages of being capable of controlling dead seedlings, rot roots and root rot, solving continuous cropping obstacles, increasing the crop yield and improving the farm product quality.