Method for rooting and thickening of fructus momordicae tissue culture seedlings

A technology of strong seedlings rooting and tissue culture seedlings, applied in the field of agricultural reproduction, can solve the problems of tissue culture seedlings being slender and brittle, low flowering and fruiting rate, increased planting risk, etc., to achieve strong external environment adaptability and lignification The effect of increasing height and improving the survival rate of transplanting

Inactive Publication Date: 2014-10-15
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Compared with the traditional potato seedlings propagated by pressure vines, the tissue cultured seedlings of Luo Han Guo have the advantages of high reproduction coefficient, no disease-carrying growth potential, and wide adaptability. phenomenon, resulting in low survival rate of colonization and low flowering and fruiting rate, thus causing economic losses and increasing planting risks

Method used

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  • Method for rooting and thickening of fructus momordicae tissue culture seedlings

Examples

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Embodiment 1

[0021] A method for rooting strong seedlings of Momordica grosvenori tissue culture seedlings, comprising the following steps:

[0022] (1) Induction culture of tissue culture seedlings: Take the sterilized budded stem section of the three-year-old Luo Han Guo female plant and inoculate it on the induction medium to induce clustered buds. The formula of the induction medium is MS+BA 0.8mg / L+NAA 0.08mg / L+sucrose 2%, pH 5.8.

[0023] (2) Proliferation and subculture of tissue culture seedlings: Inoculate clustered buds in the proliferation subculture medium and culture for more than 4 times, wherein the value-proliferation subculture medium is MS+BA 0.2mg / L+NAA 0.06mg / L+sucrose 2% , the pH value is 5.7;

[0024] (3) Rooting culture of strong seedlings of Luo Han Guo tissue cultured seedlings: inoculate the single-bud stem section of the aseptically multiplied subcultured tissue cultured seedlings in the rooting medium of strong seedlings for cultivation, the culture temperatur...

Embodiment 2

[0027] A method for rooting strong seedlings of Momordica grosvenori tissue culture seedlings, comprising the following steps:

[0028] (1) Induction culture of tissue culture seedlings: Take the sterilized budded stem section of the three-year-old Luo Han Guo female plant and inoculate it on the induction medium to induce clustered buds. The formula of the induction medium is MS+BA 0.8mg / L+NAA 0.08mg / L+sucrose 2%, pH 5.8.

[0029] (2) Proliferation and subculture of tissue culture seedlings: Inoculate clustered buds in the proliferation subculture medium and culture for more than 4 times, wherein the value-proliferation subculture medium is MS+BA 0.2mg / L+NAA 0.06mg / L+sucrose 2% , the pH value is 5.7;

[0030] (3) Rooting culture of strong seedlings of Luo Han Guo tissue cultured seedlings: inoculate the single-bud stem section of the aseptically multiplied subcultured tissue cultured seedlings in the rooting medium of strong seedlings for cultivation, the culture temperatur...

Embodiment 3

[0032] A method for rooting strong seedlings of Momordica grosvenori tissue culture seedlings, comprising the following steps:

[0033] (1) Induction culture of tissue culture seedlings: Take the sterilized budded stem section of the three-year-old Luo Han Guo female plant and inoculate it on the induction medium to induce clustered buds. The formula of the induction medium is MS+BA 0.8mg / L+NAA 0.08mg / L+sucrose 2%, pH 5.8.

[0034] (2) Proliferation and subculture of tissue culture seedlings: Inoculate clustered buds in the proliferation subculture medium and culture for more than 4 times, wherein the value-proliferation subculture medium is MS+BA 0.2mg / L+NAA 0.06mg / L+sucrose 2% , the pH value is 5.7;

[0035] (3) Rooting culture of strong seedlings of Luo Han Guo tissue cultured seedlings: inoculate the single-bud stem section of the aseptically multiplied subcultured tissue cultured seedlings in the rooting medium of strong seedlings for cultivation, the culture temperatur...

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Abstract

The invention discloses a method for rooting and thickening of fructus momordicae tissue culture seedlings and relates to the field of agricultural breeding methods. The method comprises the following steps: induction culture of tissue culture seedlings, multiplication and successive transfer culture of the tissue culture seedlings, thickening and rooting culture of fructus momordicae tissue culture seedlings, hardening-seedling of the thickened and rooted tissue culture seedlings and transplanting for survival. The formula of a thickening and rooting medium adopted by the method has a better synergistic effect, can achieve the purposes of thickening and rooting, meets the transplant requirement and has strong adaptability to the outside environment, thereby improving the transplant survival rate. The tissue culture seedlings obtained by adopting the method are thick and strong, high in disease resistance and lignification degree and can reach the out-planting transplant survival rate of more than 98%.

Description

technical field [0001] The present invention relates to the field of agricultural propagation methods, in particular to the field of the propagation method of Luo Han Guo, and in particular to a method for rooting of Luo Han Guo tissue culture seedlings and strong seedlings. Background technique [0002] Luo Han Guo (Siraitia grosvenorii) is a perennial liana of Cucurbitaceae. Luo Han Guo has the effects of clearing away heat and nourishing the lungs, relieving summer heat and promoting body fluid, smoothing the intestines and laxative, clearing the throat and relieving coughs. It is also a good fruit for beverages and seasonings. Luo Han Guo is rich in pure protein, crude protein, multivitamins, fructose and more than 20 kinds of trace elements. It also has anti-aging and anti-tuberculosis effects. [0003] Luo Han Guo is dioecious, and it is difficult to distinguish between male and female at the seedling stage, so the production has always been carried out by pressing th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 袁红娟
Owner GUANGXI UNIV
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