Method for obtaining virus-free sweet potato seedlings through high-temperature-variable temperature processing

A technology of variable temperature treatment and virus-free seedlings, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of unsatisfactory detoxification effect, difficult to clean the medium, and difficult to peel off the stem tips, etc., to achieve detoxification Good poison effect, good passivation effect, easy to strip effect

Active Publication Date: 2019-02-15
QINGDAO AGRI UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

Choose the above methods, grow and cultivate under normal conditions, because the material for detoxification grows slowly, metabolism is not strong, the detoxification effect is poor by stripping the shoot tip, the shoot tip is not easy to strip, and the regeneration rate is low
In addition, for some old varieties with severe viral diseases, the detoxification effect of conventional detoxification methods is not ideal.
[0005] In addition, the existing sweet potato seedlings grow on solid MS medium before domestication and transplanting, but the medium is difficult to clean when transplanting, and it is easy to be infected with bacteria.
It is easy to damage the roots when transplanting, and the virus-free seedlings are slow to grow

Method used

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  • Method for obtaining virus-free sweet potato seedlings through high-temperature-variable temperature processing
  • Method for obtaining virus-free sweet potato seedlings through high-temperature-variable temperature processing
  • Method for obtaining virus-free sweet potato seedlings through high-temperature-variable temperature processing

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Embodiment 1

[0034] The method for preparing sweet potato virus-free seedlings by high temperature-variable temperature treatment of the present invention specifically comprises the following steps:

[0035] 1. Treatment of sweet potato pieces before germination

[0036] Select sweet potato varieties Beijing 553 and Yanshu 25, which have severe viral diseases in the seedling stage in the field. After harvesting in autumn, take potato pieces without insects and damage, and expose them to the sun for 2-3 days.

[0037] 2. Germination

[0038] Wash the above-mentioned potato pieces with clean water, put them into sand, cover the potato pieces with sand with a thickness of 1-2 cm, and put them into an incubator at 28°C for germination. Cultivate in the dark for 1 week before germination, and after germination, light for 13 hours a day, 1000-2000lx.

[0039] 3. Shoot tip stripping and cultivation

[0040] (1) Disinfection of materials: when the sprout grows to more than 10cm, the virus disea...

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Abstract

The invention discloses a method for obtaining virus-free sweet potato seedlings through high-temperature-variable temperature processing, and belongs to the technical field of virus-free sweet potatoseedling culturing. According to the method, high-temperature processing of 40-45 DEG C is conducted on rooted sweet potato seedlings for five hours every day, and the processing is conducted continuously for 30-40 days. The method has the advantages that the passivating effect on viruses is high, a stripped stem tip has a long length, the stripping is easy, the efficiency is high, the growth ofan adventitious bud is fast, the rooting quality of regenerating seedlings is good, the seedlings are strong, and the survival rate is high.

Description

technical field [0001] The invention belongs to the technical field of cultivating virus-free sweet potato seedlings, and in particular relates to a method for obtaining virus-free sweet potato seedlings through high-temperature-variable temperature treatment. Background technique [0002] Sweet potato is an important food, feed and industrial raw material processing crop, and occupies an important position in agriculture and national economy. Sweet potato is rich in nutrition. With the improvement of people's health awareness, it has become more and more popular in recent years. Sweet potato is a root crop, which uses vegetative body for reproduction in the production process, so it is vulnerable to virus infection, and the virus density will become higher and higher with the extension of the cultivation period of the bred varieties. More than 20 viruses have been reported to infect sweet potato. There are mainly sweet potato feather mottle virus (SPFMV), sweet potato lat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01G7/06A01H4/00
CPCA01G7/06A01G31/00A01H4/001A01H4/008
Inventor 隋炯明王晶珊李恩广朱虹王霞郑春花杨雪赵春梅乔利仙
Owner QINGDAO AGRI UNIV
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