Method for obtaining virus-free sweet potato seedlings through high-temperature-variable temperature processing
A technology of variable temperature treatment and virus-free seedlings, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of unsatisfactory detoxification effect, difficult to clean the medium, and difficult to peel off the stem tips, etc., to achieve detoxification Good poison effect, good passivation effect, easy to strip effect
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[0034] The method for preparing sweet potato virus-free seedlings by high temperature-variable temperature treatment of the present invention specifically comprises the following steps:
[0035] 1. Treatment of sweet potato pieces before germination
[0036] Select sweet potato varieties Beijing 553 and Yanshu 25, which have severe viral diseases in the seedling stage in the field. After harvesting in autumn, take potato pieces without insects and damage, and expose them to the sun for 2-3 days.
[0037] 2. Germination
[0038] Wash the above-mentioned potato pieces with clean water, put them into sand, cover the potato pieces with sand with a thickness of 1-2 cm, and put them into an incubator at 28°C for germination. Cultivate in the dark for 1 week before germination, and after germination, light for 13 hours a day, 1000-2000lx.
[0039] 3. Shoot tip stripping and cultivation
[0040] (1) Disinfection of materials: when the sprout grows to more than 10cm, the virus disea...
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