Method for promoting rooting of alfalfa tissue culture seedlings

A technology for tissue cultured seedlings and alfalfa, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problem of long rooting period of alfalfa tissue cultured seedlings, affecting the efficiency of alfalfa tissue cultured seedlings, and low survival rate of transplanting and other problems, to achieve the effect of improving the speed and quality of reproduction, well-developed lateral roots, and good development

Inactive Publication Date: 2014-06-11
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Alfalfa tissue-cultured seedlings show disadvantages such as low rooting rate, slow growth, and few lateral roots in general solid rooting medium, which lead to problems such as long rooting period of alfalfa tissue-cultured seedlings, cumbersome steps, and low transplanting survival rate, which in turn affects Obtaining efficiency of alfalfa tissue culture seedlings (or Agrobacterium-mediated transgenic seedlings)

Method used

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  • Method for promoting rooting of alfalfa tissue culture seedlings
  • Method for promoting rooting of alfalfa tissue culture seedlings
  • Method for promoting rooting of alfalfa tissue culture seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A method for promoting rooting of alfalfa tissue culture seedlings, comprising the steps of:

[0036] Step (A), preparation such as figure 1 and figure 2 The solid rooting medium 1 of the hollow cylindrical shape shown;

[0037] Step (B), inoculate the rootless or a little rooted shoots obtained from the differentiation of alfalfa callus on the upper end surface of the solid rooting medium 1, and make the roots of the alfalfa seedlings grow along the hollow cylinder The inner wall of the body-shaped solid rooting medium 1 grows in a semi-suspension downward;

[0038] Step (C), seal with a sealing film after inoculation, put into the tissue culture room for rooting culture, after 1-2 weeks, when the roots of the alfalfa seedlings grow to more than 5cm, the seedlings are hardened and transplanted.

Embodiment 2

[0040] Step (A), preparation such as figure 1 and figure 2The solid rooting medium 1 of the hollow cylindrical shape shown;

[0041] Table 11 / 2 SH9 solid rooting medium formula

[0042] compound concentration MgSO 4 ·7H 2 o 0.375mM KNO 3 14mM (NH 4 )2SO 4 1.75mM CaCl 2 2H 2 o 0.55mM K H 2 PO 4 1.5mM MnSO 4 ·H 2 o 3μM

[0043] h 3 BO 3 40μM ZnSO 4 ·7H 2 o 1.75μM KI 3μM Na 2 MoO 4 2H 2 o 0.5μM CuSO 4 ·5H 2 o 0.4μM CoCl 2 ·6H 2 o 0.2μM niacin 20μM Vitamin B1 7.5μM Vitamin B6 12μM FeSO 4 0.19mM Inositol 0.275mM sucrose 1% agar 0.9%

[0044] The solid rooting medium 1 contains 1 mg / mL of indole butyric acid prepared with 75% alcohol, and the final concentration of indole butyric acid in the solid rooting medium 1 is 0.4 mg / L when it is in a high-temperature liquid state. The preparation meth...

Embodiment 3

[0050] The difference with Example 2 is: the final concentration of indolebutyric acid in solid rooting medium 1 is 0.2 mg / L when it is in a high-temperature liquid state, and the height of solid rooting medium 1 is 3 / 5 of the height of tissue culture tube 2 , on the solid rooting medium 1 in each tissue culture tube 2, inoculate 4 alfalfa seedlings to be rooted with a height of 1~2cm, and others are the same as in Example 2.

[0051] Table 2 is taking the rooting of Medicago truncatula tissue culture seedlings as an example, using the comparison of the inventive method and the conventional embedded rooting method;

[0052] Table 2 Comparison of the effects of two rooting methods after rooting culture of Medicago truncatula tissue culture seedlings for 2 weeks

[0053] rooting method Rooting rate root growth rate average root length Number of side roots Stem height Transplanting survival rate The method of the invention 95% 30mm / week 50mm 3~10...

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Abstract

The invention discloses a method for promoting rooting of alfalfa tissue culture seedlings. The method comprises the following steps: A, preparing a solid rooting culture medium (1) in a hollow cylindrical shape; B, inoculating the upper end surface of the solid rooting culture medium (1) with alfalfa seedlings obtained by tissue culturing, and enabling the roots of the alfalfa seedlings to grow downwards in a semi-hanging manner along the inner wall of the solid rooting culture medium (1) in the hollow cylindrical shape; and C, sealing by a sealing membrane after inoculating, performing rooting culture in a tissue culturing chamber, and performing seedling hardening and transplanting 1-2 weeks later when the roots of the alfalfa seedlings grow to over 5cm. According to the method for promoting rooting of alfalfa tissue culture seedlings, the alfalfa tissue culture seedlings are subjected to semi-hanging rooting culture through the solid culturing medium with a specific structure for the first time, and an efficient alfalfa rooting culture system is established by selecting appropriate rooting culture medium and rooting agent. The problems that the alfalfa tissue culture seedlings are slow in rooting, fewer in side roots, not developed in root system, low in transplanting survival rate and the like can be solved.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a method for promoting rapid rooting of alfalfa tissue culture seedlings. Background technique [0002] Alfalfa is a general term for plants of the genus Medicago in the leguminous family, which is an annual or perennial flowering plant. There are many kinds of alfalfa, the most famous of which are alfalfa (Medicago sativa) as pasture and alfalfa (Medicago truncatula) as a model plant of legume. Alfalfa is a perennial plant known as the king of forage. The alfalfa planting area in the world exceeds 10 million hectares, and the annual output of alfalfa hay exceeds 200 million tons. It is very rich in protein and is the best pasture for feeding dairy cows. Alfalfa can also be used as a vegetable for human consumption. It has high nutritional value and has the effects of clearing the spleen and stomach, benefiting the large and small intestines, and lowering bladder stones. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 龙瑞才李明娜杨青川康俊梅张铁军
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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