39results about How to "Short rooting time" patented technology

The invention relates to the technical field of plant propagation, and provides a method for constructing a cutting orchard for a crowned trunk embedding method of cunninghamia lanceolata. The methodcomprises the following steps: selecting a nursery ground, carrying out soil preparation and applying a base fertilizer, planting seedlings, shaping the scion cutting mother trees, constructing a scion cutting surface and carrying out hormone treatment, and constructing a photosynthesis surface and carrying out hormone treatment. The method solves the problems that the scion quality is poor, the rejuvenation degree is low, the yield is low, and the service life of the cutting orchard is short in other types of cunninghamia lanceolata cutting orchards.

The invention discloses a method of raising ormosia seedlings by cutting. The method includes the steps of preparing cutting beds, to be specific, building the cutting beds in a greenhouse, providing each cutting bed with a plastic shed, mixing well fresh yellow soil, fine sand and nutrient soil proportionally to obtain cutting substrate, spreading the cutting substrate on the cutting beds, and sterilizing the cutting beds with potassium permanganate before cuttage; collecting cuttings, to be specific, using shoots which grow robust, are free of disease and pest damage and are already lignified, as cuttings, cutting flat the upper end of each stem, and cutting a 45-degree slope at the lower end of each stem; treating the cuttings, to be specific, soaking lower end cuts of the cuttings into composite rooting agent solution, and treating the shoots with sterilizing liquid for standby; performing cuttage, to be specific, inserting the cuttings into the cutting substrate, making sure the lower ends of the cuttings are in tight contact with the cutting substrate, and performing full irrigation; performing post-cuttage management, to be specific, keeping the interior of bow film at 20 DEG C to 28 DEG C under the humidity of 85% to 90% to make sure the cuttings and their leaves are glossy. The method has the advantages that rooting time is short, rooting rate is high, survival rate of seedlings is high and the method is suitable for popularization and application in production practice.

The invention relates to the field of polygonatum sibiricum cultivation, in particular to a transplanting method of polygonatum sibiricum tissue culture seedlings. According to the method, the survival rate of the tissue culture seedlings is increased by taking the polygonatum sibiricum tissue culture seedlings as materials and taking a series of measures such as selection and disinfection of a transplanting substrate, disinfection of a transplanting container and agentia and management of a seedling stage environment and water after transplanting. The provided polygonatum sibiricum transplanting technology is adopted for production, the method is simple and convenient, the medicinal value is increased, the survival rate of transplanting is high, the production method can be reduced, and the method is of great significance to the medicinal species protection.

The invention relates to a hollow heating seedling cultivating box and application thereof, and belongs to a method for cultivating seedlings of trees. A box of which the bottom internal diameter is 200cm*100cm and the height is 30cm is made of color-steel complex sheets, geothermal lines of which resistances are 33ohm/m are laid on the bottom of the box, and then the seedling cultivating box which is used for heating and controlling temperature is made. Inside the seedling cultivating box, three expanded polystyrene foam sheets of which the length, width and height are 199cm*3cm*10cm are erected on the two longitudinal sides and the middle part, cover plates of the seedling cultivating box in sizes of 99cm*3cm*10cm are paved on the three expanded polystyrene foam sheets in sequence, cutting slips are inserted in small semicircular grooves while the cover plates are paved, the roots of the cutting slips appropriately contact with a black mulching film, and the small grooves of the two adjacent foam sheets are butted with each other to hold the cutting slips until that the cutting slips are fully inserted in the whole seedling cultivating box. According to the method provided by the invention, the rooting rate of the seedling is more than 95%, the root quantity is large, the roots are uniformly distributed, second-grade lateral roots are easy to cultivate, and the roots are mature. Compared with conventional seedbeds, the rooting rate and the number of the plant roots are greatly improved; more importantly, 80% of matrix is saved, so that the seedling cost is greatly reduced.

The invention discloses an air-layering rooting and seedling-growing promoting method for mulberries. The air-layering rooting and seedling-growing promoting method for mulberries includes the following steps of branch selecting and girdling, mud packaging, rooting cultivating and in-vitro seedling growing; rooting is induced by adding rooting powder into packaged mud and the moisturizing measure, and the survival seedling rate is increased. The air-layering rooting and seedling-growing promoting method for mulberries is easy to operate; mud is covered with films, moisture in mud is prevented from being evaporated, moist of matrixes is kept, and rooting is further facilitated; limitation of varieties, tree shapes and branches of mulberries do not exist, and almost all mulberries can root and grow seedlings with the method; cultivated roots of mulberry seedlings are good in development, particularly, main roots are sturdy, lateral roots are developed, young seedlings are robust, the transplanting survival rate can reach 80% or above, and the environmental adaptability is high; the rooting time is short, and the rooting quality is good; the speed and the quality of vegetative propagation of mulberries are greatly increased, and a guarantee is provided for good-seed breeding and popularization. In a word, the rooting quality of mulberries can be rapidly, efficiently and greatly improved with the method, the seedling survival rate and the transplanting survival rate of vegetative propagation of mulberries are increased.

The invention belongs to the field of tissue culture, and relates to a rootless tissue culture method of lycoris radiata and a lycoris radiata cultivating method. The rootless tissue culture method oflycoris radiata, provided by the invention comprises the steps of performing induction on bulb blocks in induction culture mediums so as to obtain adventitious buds, wherein the induction culture mediums use MS as a base, also comprise 0.5-3.0mg/L of N-(phenylmethyl)-9H-purin-6-amine, 0.5-1.5mg/L of alpha-naphthaleneacetic acid, 30g/L of cane sugar and/or white sugar and 6-8g/L of agar; and culturing the adventitious buds in propagation culture mediums so as to obtain rootless tissue culture seedlings, wherein the propagation culture mediums use MS as a base and also comprise 3.0-5.0mg/L of N-(phenylmethyl)-9H-purin-6-amine, 0.5-1.5mg/L of alpha-naphthaleneacetic acid, 0-3mg/L of forchlorfenuron, 30g/L of cane sugar and/or white sugar, 0-1mg/L of thidiazuron and 7-8g/L of agar, and numberof times of propagation culture is 1-5. Through the adoption of the rootless tissue culture method of lycoris radiata provided by the invention, the survival rate is high.

The invention discloses a matrix for the cutting propagation of cyclocarya paliurus and a processing method thereof. The matrix is composed of pennisetum purpureum, senecio scandens, garden humus soil, cattle manure crumbs, and river sand. The matrix can be applied to cutting propagation of cyclocarya paliurus and has the advantages of short rooting time and high rooting rate.

The invention discloses a method for promoting rooting of alfalfa tissue culture seedlings. The method comprises the following steps: A, preparing a solid rooting culture medium (1) in a hollow cylindrical shape; B, inoculating the upper end surface of the solid rooting culture medium (1) with alfalfa seedlings obtained by tissue culturing, and enabling the roots of the alfalfa seedlings to grow downwards in a semi-hanging manner along the inner wall of the solid rooting culture medium (1) in the hollow cylindrical shape; and C, sealing by a sealing membrane after inoculating, performing rooting culture in a tissue culturing chamber, and performing seedling hardening and transplanting 1-2 weeks later when the roots of the alfalfa seedlings grow to over 5cm. According to the method for promoting rooting of alfalfa tissue culture seedlings, the alfalfa tissue culture seedlings are subjected to semi-hanging rooting culture through the solid culturing medium with a specific structure for the first time, and an efficient alfalfa rooting culture system is established by selecting appropriate rooting culture medium and rooting agent. The problems that the alfalfa tissue culture seedlings are slow in rooting, fewer in side roots, not developed in root system, low in transplanting survival rate and the like can be solved.

The invention discloses a non-tube rootage method for a camellia oleifem tissue culture seedling. The method comprises the steps of inoculating a micro-branch which has 4-5 leaves, a slight red and brighten top leaf, a green stalk and height being 2-4cm and is obtained by performing subculture proliferation on a camellia oleifem seedling onto a root primordium induction culture medium for culturing till the base part generates a white root primordium, wherein each litre of the root primordium induction culture medium contains NAA of 3.0-5.0mg, and the rest root primordium induction culture medium is 1/2HB culture medium; taking the micro-branch with the white root primordium growing on the base part, washing the culture medium of the base part without roots and shoots, dipping with GGR (green plant growth regulator) of 200-600mg/L after filtering, instantly cutting onto a cutting medium, watering enough root fixed water, ensuring sufficient water and fertilizer supply during the growth period, and growing the micro-branch to a rooted seedling, wherein the cutting medium is formed by uniformly mixing 2-6 parts of yellow subsoil and 1-2 parts of plant ash. The method adopts non-tube rootage and breaks through the traditional mode in which the root system is induced in a tissue culture bottle, rootage and domestication are organically combined, direct rootage of the camellia oleifem tissue culture seedling outside a tissue culture bottle is realized, and the method has the advantages of short rootage time, well developed root system, absorption function and high transplantation survival rate.

The invention discloses a method for inducing rapid propagation of a cluster bud through a stem fragment of dendrobium officinale. The method comprises the following steps: step one, multiplication ofa stem fragment explant: taking a strong and high-quality plant of the annual dendrobium officinale, cutting leaves by using a sharp scalpel, washing by using running water, brushing the stem fragment by using a soft hair toothbrush, slightly stripping a white skin of the stem fragment, and using the stem fragment as the explant; soaking for 30 seconds by using 70-75% of ethyl alcohol, and washing for 3-4 times by using sterile water; processing for 10 minutes by using 10 times of bleaching water, and washing for 3-4 times by using the sterile water; and soaking by using 0.1% of mercuric chloride solution and shaking for 10-15 minutes, using the excellent individual stem fragment of the dendrobium officinale as the explant, and establishing a set of more complete stem fragment induced cluster bud tissue rapid-cultivation and propagation system. A stem fragment clone technology has the characteristics of early sprouting, short rooting time, and a rapid propagation speed, is not limitedby geographical environment and weather conditions, is not limited by seasons of seed collection, and capable of realizing annual clone production.

The invention discloses a method for rapidly inducing blueberry tissue culture seedlings to generate root primordia. The method comprises the steps that adventitious buds obtained through blueberry tissue culture serve as materials, a placement mode of one layer of cloth and one layer of seedlings is adopted, the adventitious buds are placed on cloth with a root primordium inducer soaked, light-proof heat preservation and moisture preservation induction is carried out for a period of time, then the adventitious buds are pruned into stem segments, cuttage is carried out in a rooting medium, moisture preservation and heat preservation culture is carried out, the root primordia start to appear after 5 days, the inductivity of the root primordia can reach 95% after 15 days, and the rooting rate reaches 100% after 30 days. Compared with test-tube rooting, the method has the advantages that the steps of cleaning and transplanting hardened seedling are removed, the phenomenon that a large amount of roots are damaged in the cleaning and transplanting process of the test-tube rooted seedlings can be avoided, meanwhile, a rooting inducer is easily dissolved in water, NaOH or ethyl alcohol isnot needed for dissolution promotion, the pH value does not need to be adjusted, and therefore the damage of the NaOH or the ethyl alcohol to the tissue culture seedlings can be avoided, meanwhile, the time and the cost are saved; and the method is high in rooting rate and high in transplanting survival rate, and the problems of long rooting time and low transplanting survival rate of existing blueberry tissue culture seedlings are solved.

The invention relates to a method for quickly culturing Tulipa gesneriana L.. The method comprises the following steps of seedball processing, refrigeration processing, seedball disinfection, soil preparation for fertilization, sowing in a greenhouse, seedling stage management, flower forcing, flower bud harvesting, later-stage management and the like. According to the method, culturing time can be effectively shortened, a germination rate is improved, and root taking time is shortened. During plantation, a biological organic fertilizer can be adopted to effectively improve soil fertility, sandy nutrient soil and nutrient solution can be adopted to provide enough nutrients and growth environment for the growth of the Tulipa gesneriana L.. Greenhouse plantation and cultivation can be adopted to quicken Tulipa gesneriana L. to come into the market, which is slightly affected by seasons so as to be favorable for enlarging Tulipa gesneriana L. growth environment. Meanwhile, during plantation, illumination intensity can be effectively controlled and regulated, so that the chlorophyll content and the flower percentage of Tulipa gesneriana L. leaves can be increased, the color of the Tulipa gesneriana L. is vivid, the size of a corolla is proper, a flower season is prolonged, and therefore, the method has a good practical application and market promotion value.