Outdoor transplanting method of polygonatum sibiricum tissue culture seedlings

A technology for tissue culture of seedlings and Polygonatum chinensis, applied in the directions of botanical equipment and methods, cultivation, medium, etc., can solve the problems of low reproduction coefficient, long seedling raising time, limited provenance, etc., and achieve fast growth, prolong growth period, and take root. short time effect

Active Publication Date: 2019-12-17
CHINA THREE GORGES CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Because Polygonatum seeds are difficult to collect, the seed germination rate is low, and the seedling time is long, which is not conducive to the artificial large-scale planting of Polygonatum.
At present, the reproduction of Polygonatum mainly relies on the asexual reproduction of rhizomes, but this method has a low reproduction coefficient, a large amount of seed rhizomes are used, and it is easy to deteriorate and rot, and the source of provenance is greatly limited.

Method used

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  • Outdoor transplanting method of polygonatum sibiricum tissue culture seedlings
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  • Outdoor transplanting method of polygonatum sibiricum tissue culture seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A kind of Polygonatum tissue culture seedling outdoor transplanting method, described method comprises the following steps:

[0033] S1: Transplanting substrate, transplanting test-tube seedlings, and plugs are pre-treated before transplanting:

[0034] S2: Transplanting period management:

[0035] S3: the plug seedlings are transplanted outdoors after 25-30 days;

[0036] Complete the outdoor transplanting of Polygonatum tissue culture seedlings.

[0037] Preferably, the transplanting substrate in the step S1 includes the following components in parts by weight: 1.5 parts of peat, 5 parts of river sand, 3 parts of rice bran, and 2 parts of cottonseed hulls.

[0038] Preferably, the method for treating the transplanted substrate in step S1 is: wet the substrate with 0.1% potassium permanganate aqueous solution, and cover it with a plastic film for 24 hours. Before mixing the matrix, the cottonseed hulls need to be fermented with water and high temperature for one mon...

Embodiment 2

[0050] Different substrates were changed, and the others were the same as in Example 1. The growth conditions are shown in Table 1.

[0051] Table 1 Polygonatum transplanted with different substrate growth conditions

[0052]

[0053] It can be seen from Table 1 that peat: river sand: chaff: cottonseed hull = 1.5:5:3:2, which is the best sowing substrate, the tuber growth time is 45 days, the first tuber is 1cm long, and the plant survival rate is 92% , which has a great increase in growth time and survival rate.

[0054] Table 2 embodiment 1 compares with routine processing

[0055]

[0056] As can be seen from Table 2, the optimization of the present invention and the conventional treatment shows that the rooting time is 10 days earlier than the conventional time, and the tubers of the present invention are 0.5cm larger than the conventional treatment in the same period, and the survival rate of the present invention is improved by 14% compared with the conventional t...

Embodiment 3

[0058] On the basis of Example 1, the transplanting substrate in the step S1 includes the following components by weight: 1.5 parts of peat, 5 parts of river sand, 3 parts of rice bran, 2 parts of cottonseed hulls, Maillard reaction product 1.5 servings. The production method of the Maillard reaction product is as follows: Weigh the same amount of glucosamine and xylan, dissolve them successively in 0.95%-1.2% glacial acetic acid solution, adjust the pH to 5.8-6 with 1mol / L sodium hydroxide solution, and place At 90-130°C, react for 25-35min to obtain the Maillard reaction product. See Table 3 for the growth conditions of Polygonatum Polygonatum transplanted in different substrates using the method of this embodiment.

[0059] Table 3 Rhizoma Polygonatum Transplanted Growth Situation

[0060]

[0061] As can be seen from Table 3, on the basis of the inorganic matrix of the present invention, adding a certain amount of chemical matrix, that is, the Maillard reaction produc...

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Abstract

The invention relates to the field of polygonatum sibiricum cultivation, in particular to a transplanting method of polygonatum sibiricum tissue culture seedlings. According to the method, the survival rate of the tissue culture seedlings is increased by taking the polygonatum sibiricum tissue culture seedlings as materials and taking a series of measures such as selection and disinfection of a transplanting substrate, disinfection of a transplanting container and agentia and management of a seedling stage environment and water after transplanting. The provided polygonatum sibiricum transplanting technology is adopted for production, the method is simple and convenient, the medicinal value is increased, the survival rate of transplanting is high, the production method can be reduced, and the method is of great significance to the medicinal species protection.

Description

technical field [0001] The invention relates to the field of planting technology, in particular to a method for outdoor transplanting of Polygonatum tissue cultured seedlings. Background technique [0002] Polygonatum sibiricum Delar.ex Redoute, also known as Chicken Head Polygonatum, Yellow Chicken Vegetable, etc., belongs to the perennial herb of Liliaceae (Liliaceae) Polygonatum Mill., and is a unique plant in southern China. Rhizomes cylindrical, stems climbing. The leaves are whorled, 4-6 pieces in each round, strip-lanceolate, and the apex is curled or bent into a hook. Berries black. The flowering period is from May to June, and the fruiting period is from August to September. It grows in areas with an altitude of 500 meters to 1200 meters. It is shade-loving, cold-resistant, and afraid of drought. It grows in damp mountain shrubs and forest edge grasses. Polygonatum, its rhizome, has the effects of nourishing the lungs and nourishing yin, nourishing the middle an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01G24/28A01G24/12A01G24/25C05G3/00
CPCA01G31/00A01G24/28A01G24/12A01G24/25C05D9/00C05G3/00C05F5/002C05F11/02
Inventor 望雄英张国禹黄桂云吴锦华汪磊吴笛张海波胡梅香马晓波邱利文陈会员张定军杨兰芳张俊李翩翩镇巧玲李林宝
Owner CHINA THREE GORGES CORPORATION
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