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Peony rooting culture method

A technology of rooting culture and rooting medium, which is applied in the field of improving the rooting rate and survival rate of peony, can solve the problems of low survival rate, low rooting rate, and inability to produce seedlings, so as to improve the rooting rate and elongation of seedlings, and improve the survival rate. The effect of short rooting time

Inactive Publication Date: 2016-05-04
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is: rooting of peony is difficult, and the low rooting rate leads to low survival rate, which cannot produce seedling emergence. The present invention provides a tissue culture method for rapid rooting and propagation of peony

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] A rooting culture method for peony: peel off several layers of bud scales of peony buds, wash them under tap water for 30 minutes, soak and rinse them with carbendazim for 8-10 minutes, then sterilize them with 70% alcohol on a super-clean workbench for 20-30 seconds, and then Sterilize with 2% sodium hypochlorite or 0.1% mercury liter for 8 minutes, rinse with sterile water 3 to 5 times, peel off the remaining bud scales, cut off the base part of the tissue, inoculate the remaining part on the solid medium, The formula of described solid culture medium is: MS+contains Ca 2+ WPM+IBA0.5mg / L+6-BA0.3mg / L+PVP1g / L+sugar 30g / L+agar 7g / L, at pH=5.8, temperature 24±1℃, light intensity 2000Lx, light time 12h / d Induce culture for 30 days to obtain rootless seedlings, and transfer the rootless seedlings to the rooting medium. The formula of the rooting medium is 1 / 2MS+IBA1.0mg L -1 + sucrose 30g·L -1 , at a pH of 5.8, a temperature of 24±1° C., a light intensity of 2000 Lx, and ...

Embodiment 2

[0012] A rooting culture method for peony: peel off several layers of bud scales of peony buds, wash them under tap water for 30 minutes, soak and rinse them with carbendazim for 8-10 minutes, then sterilize them with 70% alcohol on a super-clean workbench for 20-30 seconds, and then Sterilize with 2% sodium hypochlorite or 0.1% mercury liter for 8 minutes, rinse with sterile water 3 to 5 times, peel off the remaining bud scales, cut off the base part of the tissue, inoculate the remaining part on the solid medium, The formula of described solid culture medium is: MS+contains Ca 2+ WPM+IBA0.5mg / L+6-BA0.3mg / L+PVP1g / L+sugar 30g / L+agar 7g / L, at pH=5.8, temperature 24±1℃, light intensity 2000Lx, light time 12h / d Induce and cultivate for 30 days to obtain rootless seedlings, and transfer the rootless seedlings to the rooting medium. The formula of the rooting medium is WPM+IBA4mg L -1 +PVP1.0g·L -1 + sugar 30g·L -1 +Gellangum2g·L -1 , cultivated under the conditions of pH 5.8, ...

Embodiment 3

[0014] A rooting culture method for peony: peel off several layers of bud scales of peony buds, wash them under tap water for 30 minutes, soak and rinse them with carbendazim for 8-10 minutes, then sterilize them with 70% alcohol on a super-clean workbench for 20-30 seconds, and then Sterilize with 2% sodium hypochlorite or 0.1% mercury liter for 8 minutes, rinse with sterile water 3 to 5 times, peel off the remaining bud scales, cut off the base part of the tissue, inoculate the remaining part on the solid medium, The formula of described solid culture medium is: MS+contains Ca 2+ WPM+IBA0.5mg / L+6-BA0.3mg / L+PVP1g / L+sugar 30g / L+agar 7g / L, at pH=5.8, temperature 24±1℃, light intensity 2000Lx, light time 12h / d Induce and cultivate for 30 days to obtain rootless seedlings, and transfer the rootless seedlings to the rooting medium. The formula of the rooting medium is WPM+IAA3mg / L+caffeic acid 1mg / L+PVP1g / L+LH200mg / L+sugar 30g / L+Gellangum 2g / L, cultured for 30-35 days under the ...

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Abstract

The invention discloses a peony rooting culture method which comprises the following steps: stripping a few layers of bud scales from peony bulbil, washing with tap water for 30min and soaking and rinsing with carbendazim for 8-10min; sterilizing with 70% alcohol for 20-30s on an ultra-clean workbench; sterilizing again with 2% sodium hypochlorite or 0.1% mercury bichloride for 8min; washing with sterile water for 3-5 times; stripping the rest bud scales; cutting off some tissues of the base, and inoculating the rest part to a solid culture medium to obtain rootless seedlings; and transferring the rootless seedlings to a rooting culture medium to obtain peony seedlings. By adopting the method disclosed by the invention, the rooting rate and elongation of the seedlings can be effectively increased, so that the peony rooting time is shorter, and the survival rate is improved. The rooting time is shortened to 10 days, the maximum root length is 25.58mm, the rooting rate is over 70%, and the root number is 4.8; however, the contrasted rooting time is 20 days, the rooting rate is 10%, the maximum root length is 18.06, and the root number is 2.

Description

technical field [0001] The invention is a method for improving the rooting rate and survival rate of peony through different hormones. Background technique [0002] Peony belongs to the deciduous shrubs of Paeoniaceae Paeoniaceae, and has been cultivated as a flower for about 1600 years. The traditional propagation methods of peonies include seed propagation and vegetative propagation based on grafting, branching, and cuttings. However, the traditional propagation methods have great limitations: seed propagation can obtain a large number of seedlings in a short period of time, but the fruitful plant varieties are single-petal Mainly, the double-petal varieties are not fruitful or very few, and the seedlings vary greatly, so it is not suitable to maintain the good character of the mother plant. Peony not only occupies an important position in the domestic flower market, but also is an important export flower in my country. Industrial production is the main direction of futur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 何松林贺丹王政尚文倩刘保国解梦珺
Owner HENAN AGRICULTURAL UNIVERSITY
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