Method for rapidly inducing root primordia of blueberry tissue culture seedlings

A technology for tissue culture seedlings and blueberries, which is applied in the field of horticultural plant breeding, can solve the problems of less rooting, long induction period, and affecting rooting efficiency, and achieve the effects of short rooting time, high rooting rate, and promotion of survival

Active Publication Date: 2021-02-19
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the blueberry rooting induction outside the bottle is to directly induce adventitious roots, which has a long induction period and a small number of roots, which seriously affects the rooting efficiency.

Method used

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  • Method for rapidly inducing root primordia of blueberry tissue culture seedlings
  • Method for rapidly inducing root primordia of blueberry tissue culture seedlings
  • Method for rapidly inducing root primordia of blueberry tissue culture seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Determination of the type of inducer

[0020] Adventitious buds were treated with 4 kinds of inducers in Table 1 for 24 hours, the concentration was 100mg / L, and the induction rate of root primordium was investigated respectively on 10d and 15d. The results found that the induction effect of sodium indolebutyrate was the best, The induction effect of naphthaleneacetic acid was the worst, and the induction effect of indolebutyric acid and potassium indolebutyrate was not significantly different, but the stems treated with potassium indolebutyrate had more root primordia and covered a larger area. Therefore, sodium indolebutyrate has the best effect on inducing root primordia, followed by potassium indolebutyrate. You can try to use the ratio of the two to enhance the induction effect.

[0021] Table 1 Effects of different kinds of inducers on the induction rate of root primordia

[0022]

[0023] Note: The lowercase letters are the difference at the level of p<0.05 (...

Embodiment 2

[0025] Determination of the ratio of inducer

[0026] Adventitious buds were treated with the 5 kinds of reagents in Table 2, the concentration was 100mg / L, and the induction rate of root primordia was investigated on 15 days, and the rooting rate was investigated on 30 days. The results showed that the combined use of sodium indole butyrate and potassium indole butyrate was effective It is better than the effect of using alone in the implementation case 1. When the ratio of the two is 3:1 or 4:1, the effect is the best, the root primordium induction rate is the highest, and the rooting rate can reach 100%. Therefore, the optimal ratio range of the two is sodium indole butyrate: potassium indole butyrate = 3:1~4:1.

[0027] Table 2 The effect of the ratio of sodium indolebutyrate: potassium indolebutyrate on the induction rate and rooting rate of root primordia

[0028] Sodium Indole Butyrate: Potassium Indole Butyrate 15d root primordium induction rate / % 30d ro...

Embodiment 3

[0030] Determination of inducer concentration

[0031] Sodium indolebutyrate: Potassium indolebutyrate=3:1 is the inducer, and 6 kinds of inducer concentrations in Table 3 are adopted. The results show that the inducer concentration is 25mg / L when the root primordia induction rate and rooting rate are the lowest, At 50-150mg / L, the root primordium induction rate and rooting rate had no significant difference and were the highest, so the optimal concentration range of the inducer was 50-150mg / L.

[0032] Table 3 Effect of inducer concentration on root primordia induction rate and rooting rate

[0033] Inducer concentration mg / L 15d root primordium induction rate% 30d rooting rate% 25 50.67d 59.33c 50 97.67a 100a 100 100a 100a 150 100a 100a 200 85.67b 87.33b 250 62.33c 65.67c

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Abstract

The invention discloses a method for rapidly inducing blueberry tissue culture seedlings to generate root primordia. The method comprises the steps that adventitious buds obtained through blueberry tissue culture serve as materials, a placement mode of one layer of cloth and one layer of seedlings is adopted, the adventitious buds are placed on cloth with a root primordium inducer soaked, light-proof heat preservation and moisture preservation induction is carried out for a period of time, then the adventitious buds are pruned into stem segments, cuttage is carried out in a rooting medium, moisture preservation and heat preservation culture is carried out, the root primordia start to appear after 5 days, the inductivity of the root primordia can reach 95% after 15 days, and the rooting rate reaches 100% after 30 days. Compared with test-tube rooting, the method has the advantages that the steps of cleaning and transplanting hardened seedling are removed, the phenomenon that a large amount of roots are damaged in the cleaning and transplanting process of the test-tube rooted seedlings can be avoided, meanwhile, a rooting inducer is easily dissolved in water, NaOH or ethyl alcohol isnot needed for dissolution promotion, the pH value does not need to be adjusted, and therefore the damage of the NaOH or the ethyl alcohol to the tissue culture seedlings can be avoided, meanwhile, the time and the cost are saved; and the method is high in rooting rate and high in transplanting survival rate, and the problems of long rooting time and low transplanting survival rate of existing blueberry tissue culture seedlings are solved.

Description

technical field [0001] The invention belongs to the field of horticultural plant breeding, and in particular relates to a method for rapidly inducing root primordia to be produced outside the bottle of blueberry tissue culture seedlings. Background technique [0002] Blueberry is known as the king of berries. It has high nutritional value, especially rich in anthocyanins. It has health effects such as eye protection, beauty and beauty, and anti-aging. Because of its edible and medicinal value, it is listed by the International Food and Agriculture Organization. Top 5 Healthy Foods. Therefore blueberry is more and more subject to liking of people, and market demand is very big. [0003] The increase in the demand for blueberry fruit has promoted the popularity of the market for blueberry seedlings. In order to meet the market demand, blueberry tissue culture industrial seedling technology came into being. Because the blueberry root system is a shallow root system, with only...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001Y02P60/21
Inventor 王小敏吴文龙李维林樊苏帆闾连飞张春红
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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