Method for inducing rapid propagation of cluster bud through stem fragment of dendrobium officinale

A technology of Dendrobium officinale and stem segments, which is applied in the field of rapid reproduction of Dendrobium officinale, can solve the problems of depletion of wild resources, low reproductive ability, lack of endosperm, etc., and achieves the effects of simplifying production procedures, maintaining the characteristics of the female parent, and increasing the reproduction speed.

Inactive Publication Date: 2019-01-01
翁源县天下泽雨农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved in the present invention is to overcome the existing defects, and provide a method for rapid propagation of stem segments of Dendrobium candidum to induce clustered buds to solve the problem of the lack of endosperm in the seeds of Dendrobium candidum under the natural conditions mentioned above. Extremely low, coupled with the long-term unrestrained mining of human beings, the wild resources are becoming increasingly depleted. The Dendrobium officinale currently in the market is basically a cultivated variety, but its output still cannot meet the current medicinal needs. In order to protect the existing wild species quality resources, to maintain a stable supply of the provenance of Dendrobium candidum, and the research on rapid propagation of Dendrobium candidum is increasing.

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  • Method for inducing rapid propagation of cluster bud through stem fragment of dendrobium officinale
  • Method for inducing rapid propagation of cluster bud through stem fragment of dendrobium officinale

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Embodiment 1

[0041] A kind of stem segment of Dendrobium officinale induces clustered bud rapid propagation method, comprises the following steps:

[0042] Step 1. Proliferation of stem explants: Take a strong and high-quality annual Dendrobium officinale plant, cut off the leaves with a sharp scalpel, rinse with running water, brush the stem with a soft-bristled toothbrush, and gently peel off the white skin of the stem. Use stem segments as explants; soak in 70-75% alcohol for 30 seconds, rinse with sterile water for 3-4 times; treat with 10 times bleach for 5 minutes, rinse with sterile water for 1-3 times; then use Soak in 0.1% mercuric chloride solution and shake for 5 to 10 minutes, rinse with sterile water for 1 to 3 times, dry the water, cut off both ends of the stem with a sterile scalpel, each stem is about 1.5cm long, with 2 Inoculate one stem section per bottle of culture medium to avoid cross-contamination, and inoculate the sterilized stem section into the starting medium: Hu...

Embodiment 2

[0055] A kind of stem segment of Dendrobium officinale induces clustered bud rapid propagation method, comprises the following steps:

[0056] Step 1. Proliferation of stem explants: Take a strong and high-quality annual Dendrobium officinale plant, cut off the leaves with a sharp scalpel, rinse with running water, brush the stem with a soft-bristled toothbrush, and gently peel off the white skin of the stem. Use stem segments as explants; soak in 70-75% alcohol for 30 seconds, rinse with sterile water for 3-4 times; treat with 10 times bleach for 10 minutes, rinse with sterile water for 3-4 times; then use Soak in 0.1% mercuric chloride solution and shake for 10-15 minutes, rinse 3-4 times with sterile water, dry the water, cut off both ends of the stem with a sterile scalpel, each stem is about 1.5cm long, with 2 Inoculate one stem section per bottle of culture medium to avoid cross-contamination, and inoculate the sterilized stem section into the starting medium: Huabao No....

Embodiment 3

[0069] A kind of stem segment of Dendrobium officinale induces clustered bud rapid propagation method, comprises the following steps:

[0070] Step 1. Proliferation of stem explants: Take a strong and high-quality annual Dendrobium officinale plant, cut off the leaves with a sharp scalpel, rinse with running water, brush the stem with a soft-bristled toothbrush, and gently peel off the white skin of the stem. Use stem segments as explants; soak in 70-75% alcohol for 30 seconds, rinse with sterile water for 3-4 times; treat with 10 times bleach for 15 minutes, rinse with sterile water for 4-5 times; then use Soak in 0.1% mercuric chloride solution and shake for 15-20 minutes, rinse 4-5 times with sterile water, dry the water, cut off both ends of the stem with a sterile scalpel, each stem is about 1.5cm long, with 2 Inoculate one stem section per bottle of culture medium to avoid cross-contamination, and inoculate the sterilized stem section into the starting medium: Huabao No....

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Abstract

The invention discloses a method for inducing rapid propagation of a cluster bud through a stem fragment of dendrobium officinale. The method comprises the following steps: step one, multiplication ofa stem fragment explant: taking a strong and high-quality plant of the annual dendrobium officinale, cutting leaves by using a sharp scalpel, washing by using running water, brushing the stem fragment by using a soft hair toothbrush, slightly stripping a white skin of the stem fragment, and using the stem fragment as the explant; soaking for 30 seconds by using 70-75% of ethyl alcohol, and washing for 3-4 times by using sterile water; processing for 10 minutes by using 10 times of bleaching water, and washing for 3-4 times by using the sterile water; and soaking by using 0.1% of mercuric chloride solution and shaking for 10-15 minutes, using the excellent individual stem fragment of the dendrobium officinale as the explant, and establishing a set of more complete stem fragment induced cluster bud tissue rapid-cultivation and propagation system. A stem fragment clone technology has the characteristics of early sprouting, short rooting time, and a rapid propagation speed, is not limitedby geographical environment and weather conditions, is not limited by seasons of seed collection, and capable of realizing annual clone production.

Description

technical field [0001] The invention belongs to the technical field of rapid propagation of dendrobium candidum, and in particular relates to a method for rapid propagation of stem segments of dendrobium candidum induced clustered buds. Background technique [0002] Dendrobium officinale, also known as Tiepilan or nigella, is a perennial epiphytic herb of the genus Dendrobium orchidaceae. It is named Dendrobium officinale because of its iron-green stem. Dendrobium officinale has long been famous for its extremely high medicinal value, which has the functions of expectorant, anti-oxidation, improving immune function, lowering blood sugar and anti-cancer. Dendrobium candidum products currently on the market - Tiepi Fengdou is a processed Dendrobium candidum, and it is a rare Chinese herbal medicine with high economic value and medicinal value. Dendrobium officinale is the best product in the Dendrobium genus. It is named for its iron-green skin. For thousands of years, it ha...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈绪盛杜正丽毛明辉
Owner 翁源县天下泽雨农业科技有限公司
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