149 results about "Survival ratio" patented technology

The present invention relates to the storage agent for preservation of an animal cell or organ and preserved process. Ordinary method of cell storage is employed preserving method by freezing at extra low temperature of -196° C. and the survival ratios of cells after thawing and fusion is low, about 10 to 30%. The period of validity is a very short time of 12 to 72 hours. The storage agent can make protein stabilize to protein type storage agent, and prevent, treat and improve organ injury caused on an organ transplant operation by adding the polyphenol.

The invention discloses a method for promoting plant growth by illumination, in particular to designing a light source product with universal meaning according to the spectrum demanded by plant growth, therefore the method of supplementary illumination of plants in raising seedling of plastic tents and factories is found. The method comprises the following processes: (1) seedling preparation; (2) confirming the absorption spectrum of the plants; (3) testing the photosynthesis under different light qualities; (4) comparing and analyzing the experiments so as to confirm the light absorption of different plants; (5) designing light source equipment; (6) supplementary illumination is carried out to the plants. In the method, spectrum test equipment is used for testing the plants with different types; the light sources with different wavelengths are chosen and made according to the absorption spectrum of the plants, therefore the pertinence and efficiency of the light source can be improved efficiently. The bud ratio and the survival ratio can also be improved efficiently, the seedling state can be shortened, the biomass of seedlings can be increased, and the height of the seedlings and the number of leaves are promoted for increasing, thus shortening the growth period of the plants efficiently and improving the efficiency and benefit during raising seedling and growth.

The invention provides a sea cucumber bottom-sowing culture facility suitable for a soft seabed. The facility comprises frameworks, built-in separation nets, built-in dense nets, cuffs, a traction rope, an anchoring object and a marking floater. The frameworks are arranged in parallel and are covered with outer-wrapping netlike clothing, the frameworks are mutually connected to be of a telescopic mesh cage shape, the built-in separation nets for separating each framework into a plurality of culture units are arranged on each framework, the built-in dense net for catching and gathering sea cucumber baits is arranged in each culture unit, cuffs for taking and placing a sea cucumber is arranged on the outer-wrapping netlike clothing of each culture unit, the frameworks on two ends are respectively and sequentially connected with the traction rope and the anchoring object to be fixedly arranged in a seabed, and the marking floater is connected onto one end of the culture facility. Compared with the traditional culture facility, the culture facility provided by the invention is light and convenient, can be compressed, and is convenient for transportation, throwing and management, and the affect from a natural disaster of typhoon and the like is avoided; and by the adoption of the culture facility, the problems of easiness in entrapping by silt, poor culture effect and the like of the traditional culture facility can be solved, particle-like suspended matters in seabed water can be effectively held back as sea cucumber baits, and the survival ratio and the growth rate of the sea cucumber are improved.

The invention discloses a multifunctional transpiration inhibitor which comprises the following ingredients in percentage by weight: 35-55 percent of cellulose ether, 9-26 percent of biochemical fulvic acid powder, 1-9 percent of organic rare earth, 1-5 percent of sodium hexametaphosphate, 5-20 percent of chelating trace elements, 0-3.5 percent of polyacrylamide and 0-2 percent of Alpha-sodium acetate; wherein the organic rare earth is rare earth prepared from the chelation reaction by adopting a chelating agent; the chelating trace elements are generated by adding ionized water and the chelating agent into the combination of copper sulfate, zinc sulfate, ferrous sulfate and manganese sulfate for performing the chelation reaction; and the cellulose ether is series products of water-soluble cellulose ethers. The multifunctional transpiration inhibitor has the functions of inhibiting transpiration, resisting drought and wind, strengthening nutrition, and the like, can improve the survival ratio, the preservation ratio and the survival state of plants, further enhance the capacities of resisting stress, diseases, draught, cold and the like of the plants and achieves the aims of resisting draught and saving water, ensuring seedlings to survive as well as increasing both production and income.

The present invention relates to preparation of a municipal dewatering sludge based coating material for seal of landfill site, is mainly used for seal covering of the landfill site, and belongs to the field of environmental geotechnical engineering. The municipal dewatering sludge is employed as a basis material and modified to be used in seal covering of the landfill site. An air-out layer of the coating material for seal of landfill site has good gas guide performance; an impervious layer is not easily cracking and has low permeability; and a vegetation nutrient layer has rich nutrients and good hydrophobicity. The covering material of the present invention realizes resource utilization of municipal dewatering sludge and saves land resource; the covering material can be produced by industrialized production, has a simple construction for application to seal covering of the landfill site, and has advantages of simpleness, fastness, strong operability, high survival ratio of plants, so as to guarantee quality of seal covering of the landfill site, meet various technological indexes of seal of the landfill site, and greatly improve the landfill environment.

The invention relates to a cuttage propagation method of Virginia oak, which belongs to the technical field of plant cultivation. The method comprises the following steps: firstly, a sterilizing seedbed is built in a big-arch shelter, an arch shed is built on the seedbed, a hole pan provided with cuttage substrate is arranged in the seedbed, and the cuttage substrate is treated and sets aside; secondly, branches derived from close roots of the Virginia oaks grown for 2-4 years are selected to prune ears in February to March every year when sprouts do not and are about to burgeon, and the ear strips are pruned into branches; thirdly, the branches are treated by root stimulating agent, and the cuttage is done immediately, for the branches which then enter into the management after the cuttage; fourthly, after more than 70 percent of branches root and sprout, the hole pan is moved to forcibly exercise the plantlets outside the big-arch shelter, and the plants are transplanted into ground after being exercised for 30-35 days at the last third of the December or the last third of the February of the next year and enter the management after transplanting. The cuttage propagation adopting the method has high survival ratio and fast propagation speed. The invention is suitable for the climatic environments in most parts of Yangtze River Delta in China and has the survival ratio up to 80-90 percent, and the obtained nursery plantlets of the Virginia oaks have sound quality.

The invention discloses a nutrient solution for grafting of red kiwi and a use method of the nutrient solution. The nutrient solution comprises an I type nutrient solution and an II type nutrient solution, wherein the I type nutrient solution contains the following constituents: 1,320mg ammonium nitrate, 1,150mg nitrate, 350mg calcium chloride, 240mg magnesium sulfate, 125mg potassium dihydrogen phosphate, 32.5mg sodium ethylene diamine tetracetate, 20.3mg ferrous sulfate, 4.3mg boric acid, 0.35mg potassium iodide, 15.7mg manganese sulfate, 0.02mg copper sulfate, and 7,200mg saccharum or agar that are dissolved in every 1L water, and the II type nutrient solution contains the following constituents: 0.35mg naphthylacetic acid and 0.35mg indolebutyric acid that are dissolved in every 1L water. The nutrient solution for the grafting of the red kiwi can prolong the effective to-be-grafted time of sprouting to be grafted, increase the survival ratio and utilization ratio of the grafting and facilitate the production and popularization of kiwi planting.

The invention discloses a slope forest planting method which is mainly used for reclaimation tree-planting on the slope of a waste disposal site of an open pit in arid regions. The method mainly comprises the following steps of: selecting surface soil peeled from the open pit and nutritional soil prepared from sheep and cow dung, putting the nutritional soil in a planting basket weaved by plant wickers, planting seedlings in and putting in a seedling cultivating ditch, then irrigating, transplanting into a slope planting cave together with the planting basket after various seedlings are watered and irrigated for 3 to 18 months, filling by surface soil, well trimming the seedlings, and preserving a water hole at the periphery, wherein the seedling survival ratio after three-year watering can reach more than 88 percent. The planting basket is weaved by plant wickers, which can become fertilizer after rotting and has no pollution to environment. The root system of a tree after growing up can stabilize the soil, protect the slope and prevent soil erosion and water loss; branches and leaves at the crown can reduce the wind speed and runoff amount, prevent rain water from directly scouring the slope and protect the slope, therefore, the ecological environment of a plurality of tree and grass vegetation is formed.

The invention relates to a breeding method for grafting and cultivating other types and breeds of hibiscus economic and ornamental plants by taking an extremely salt-resistant hibiscus hamabo as a wildstock to improve the salt resistance of scion. The pot experiment proves that the growth vigor of hibiscus which is grafted and propagated by taking the hibiscus hamabo as the wildstock is exuberant,the grafting survival ratio is high (above 90 percent), the hibiscus is resistant to the soil salt above 8 thousandth, but the hibiscus propagating by cuttings is moderately damaged by 4 thousandth soil salt. The hibiscus plant after being improved can be popularized to be cultivated and applied on the wide coastal shoal, thereby improving the utilization and yield of the land resources on the coastal shoal; at the same time, the breeding method has the great significance on enriching the ecological landscape of the coastal shoal and improving the ecological environment of the coastal shoal.

The invention discloses scion root liquid of hackberry. Each liter of root liquid contains the following components in parts by weight: 1-3 parts of 6-benzyl amino adenine, 2-3 parts of ABT2 rooting powder, 15-20 parts of heteroauxin, 20-30 parts of naphthylacetic acid, 0.2-0.5 part of potassium iodide, 2-4 parts of zinc sulfate, 0.05-0.1 part of sodium molybdate, 10-20 parts of ammonium nitrate, 1-3 parts of magnesium sulfate, and 1-1.5 parts of vitamin D. A method for grafting and seeding the hackberry by the root liquid comprises the steps of: selecting scions and preparing the scions; selecting a stock and cutting the selected stock; connecting; and finally, carrying out seeding management. The root liquid disclosed by the invention is reasonable in formula, and the survival ratio of the grafted hackberry can be improved to over 90%. The grafting method disclosed by the invention is simple and convenient to operate, and easy to popularize.

The invention discloses a method for forestation by means of level trenches on separated slopes in arid-hot valleys of the Jinshajiang River, pertaining to the technical field of soil and water conservation. The method comprises the essential steps of building level trenches on separated slopes, refilling soil, setting nursery stocks and covering with grass. The method of the invention adopts measures such as utilizing the reasonable level trenches on separated slopes to accumulate nature rainfall, mixing appropriate base fertilizers in the refilling soil, etc., which greatly increases the survival ratio and preservation rate of forestation nursery stocks. The method of the invention can ensure that the survival ratio of nursery stocks of jatropha curcas and hedge acacia under the circumstances of no irrigation water is increased to more than 95 percent, which is increased by 32 percent and 18 percent respectively than the control group, and both preservation rates are increased to more than 92 percent in 1 year and are respectively 42 percent to 82 percent higher than the control group; after half a year, the tree crown growing widths of the jatropha curcas and the hedge acacia are increased by 50 cm multiplying 49 cm <2> and 32 cm multiplying 32 cm <2> respectively. The method of the invention successfully solves the problem of soil moisture during forestation in arid-hot areas of the Jinshajiang River and has practical and profound significance in revegetation and ecological reconstruction of such areas.

The invention provides a roof garden combination planting module which does not need water, electricity, artificial watering and maintenance and is energy saving and environment friendly. The module consists of five parts of a rain water collecting box, a drainage plate, a planting box, edge sealing strips and angle sealing strips. The module is particularly applied to the greening of old houses and light steel roofs, which is low in cost requirement, small in bearing and difficult in watering and maintenance. The combination planting module depends on the structural advantages and has the characteristics of plant root resistance and water storage intercommunication; on the one hand, the module can be directly arranged on roofs to be used without needing the cooperation of root resistance waterproof coiled materials of other plants, on the other hand, the module does not need a large number of water supply pipes and adaptors, thereby greatly reducing cost of a roof garden system. Depending on the advantages of the standardized function design, the combination planting module has the characteristics of high commonality of products, strong adaptability of the system, high survival ratio of plants, short construction cycle, no construction rubbish, quick and convenient arrangement, low labor intensity, low product cost and convenient large-scale flow line production, etc.

The invention relates to human sDR5-Fc recombinant fusion protein and application of the human sDR5-Fc recombinant fusion protein to preparation of medicine for preventing and treating drug induced liver injury. Through long-time study of the inventor, the protein gene sequence is selected again, and the novel human sDR5-Fc recombinant fusion protein provided by the invention is obtained. Through a great number of experiments, the human sDR5-Fc recombinant fusion protein can obviously reduce the serum transaminase level in various mouse drug induced liver injury models; the liver pathologic damage is reduced; the liver cell apoptosis rate is obviously reduced; the mouse survival ratio is improved. The human sDR5-Fc recombinant fusion protein is used as novel candidate medicine for preventing and treating the drug induced liver injury; the treatment range is wide; targets are specific; the action is special and fast; the curative effect is obvious; the safety is high; great development potential is realized.

The invention belongs to the technical field of strain cultivation and relates to a method for preparing armillaria mellea strains by using cut logs. The method comprises the steps: (A) blending materials: weighing culture materials according to a formula of a culture medium, mixing uniformly, and adding water until the water content is 55-70%, wherein the culture medium is prepared from the following ingredients in parts by weight: 8-12 parts of dry branch segments of weed-tree branches, 12-18 parts of wheat bran, 8-12 parts of maize flour, 2-4 parts of soybean meal, 1 part of white sugar, 1 part of gypsum powder and 1 part of potassium dihydrogen phosphate; (B) bagging the uniformly mixed culture materials, sterilizing and inoculating; (C) culturing: culturing the inoculated material bags at the temperature of 20-25 DEG C until the material bags are full of armillaria mellea hyphae. According to the method, the culture medium is appropriate in ingredient and ratio and balanced in nutrition; during the preparation of the armillaria mellea hyphae, the culture medium is strong in water retention and sufficient in nutrition, and the obtained armillaria mellea hyphae are thick and strong and are high in survival ratio and yield.

A kind of sensor used for monitoring, measuring stress and strain. It can solve the problems of low sensitivity, bad consistency with concrete, bad wear and monitoring precision all time of sensing component in the present building, and the problems of expensive, complex test assistant device and low survival ratio. The sensor is made up of the alertness cement basal material and many electrodes. Many electrodes with the shape of strip, net or ring are buried in the cement basal material or pasted, plated on the material surface. The sensor of the invention can not only monitor / measure the stress of cement structure but monitor / measure the strain of concrete structure, also in the water conservancy and nuclear engineer. It has the high sensitivity, good wear, consistency and high survival ratio while buried.

The invention relates to a preparation method of copper-rich yeast. In the invention, high-copper resistant yeast strains are firstly prepared: yeast strains with different biochemical characteristics are cultivated in a culture medium in a gradient copper concentration, and the ones having maximal survival ratio are used as strains to be mutagenized; the strains to be mutagenized are inoculated on the culture medium of diethyl sulfate for mutagenesis; the strains after mutagenesis are inoculated in the culture medium in a gradient copper concentration for cultivation, fungus liquid collection, filtering and drying are carried out, and the strains after mutagenesis with biomass and copper content simultaneously higher than average values are used as the high-copper resistant yeast strains. And then the high-copper resistant yeast strains are used for preparing the copper-rich yeast, and the working procedures are as follows: slant strain cultivation; first-stage liquid seed cultivation; second-stage seed cultivation; fermentation cylinder cultivation; separation drying; grinding; and products of copper-rich yeast. The additives of the copper-rich yeast prepared in the invention greatly improves the absorption utility ratio of copper in animal organisms, increases the economic benefit, and can be applied to copper addition for beasts and birds, and aquatic livestock as feed additives.

The invention discloses special feed for a juvenile alligator snapping turtle, which comprises the following components in percentage by weight: 49 percent-70 percent of fish meal, 8 percent-30 percent of bean pulp and / or meat and bone meal, 9.8 percent-23.2 percent of starch, 0.2 percent-4.7 percent of fish oil, 0 percent-6 percent of monocalcium phosphate, 0.5 percent-1 percent of vitamin, 1 percent-2 percent of mineral substance and 0.1 percent-0.3 percent of choline chloride. In the invention, the special feed for the juvenile alligator snapping turtle has the advantages of excellent raw materials and scientific nutrition preparation and can ensure that the raw materials are completely ingested by the juvenile alligator snapping turtle without generating aquaculture pollution caused by waste feed, wherein the crude protein is more than or equal to 45 percent, the crude fat is more than or equal to 6 percent, the water content is less than or equal to 10 percent, and the floating time is up to 8-10 hours; after the part of fish meal is replaced by the bean pulp and / or the meat and bone meal, the survival ratio of the alligator snapping turtle is not influenced, but various indexes, such as terminal weight, specific growth ratio, weight growth ratio and the like are obviously improved, and the production cost is greatly reduced.

The invention relates to a small-waterplane high-density semi-flowing cultivating technology of paddlefish, 5 to 6 paddlefish fries are put in per cubic meter and matched with 60 chub fries and 100 crucian fries, sweet wormwood herb is put into a fishpond at the front stage so as to cultivate plankton in the fishpond as main fry feed, expanded feed is then put into the fishpond as main feed of the paddlefish at the later period, and fishpond water is replaced on time during the cultivation period. The invention is free from the overcritical requirement on a large-waterplane fishpond for cultivating the paddlefish, the paddlefish can be cultivated by a large scale at the area that is short of large-waterplane fishpond, and the paddlefish has high survival ratio, high growth speed and docile temperament and is easy to catch.

The invention relates to a method for culturing nucleated pearls and belongs to the field of pearl culture. The method comprises the following steps: 1, taking a shell and preparing a nucleus I, treating and then manufacturing a pallium cell tablet; 2, adhering the pallium cell tablet to the surface of the nucleus I, implanting into the body of a mussel and culturing; 3, taking the nucleus I out of the mussel body, treating a nucleus II, implanting the nucleus II into the mussel body, culturing and forming a nucleated pearl in a pearl sac of the mussel body. By the method for culturing the nucleated pearls, the survival ratio of pearl culturing mussels can be greatly increased.

The invention relates to a method for breeding parent fish of paralichthys lethostigma. The method comprises desalination culture and salinization culture of standby parent fish as well as intensive culture of the parent fish. Specially made feed is fed during the intensive culture of the parent fish, wherein, the weight percentages of main materials in the feed are the following: 30-40 percent of Gobiidae or ammodytes, 15-20 percent of cuttlefish or sleeve-fish, 15-20 percent of sea cucumber and 20-30 percent of bastard halibut powder; the weight percentages of additives accounting for the main materials are the following: 1-2 percent of parent fish hardening agent, 0.4 percent of Vc and 0.4 percent of Ve; the feed particle size is 9mm-11mm. During light and temperature regulation and control, four bulbs are suspended over each parent fish culture tank with two bulbs of 60W and two bulbs of 150W suspended on the cross to obtain even light; water temperature is raised to 17 DEG C from 14.5 DEG C. Based on biological characteristics of wide optimum salinity of the paralichthys lethostigma, the invention makes use of F1 replacing fingerlings to culture standby parent fish in fresh water after desalination, and after salinization the standby parent fish is intensively cultured to obtain sexually mature egg-laying parent fish, thereby effectively solving the problem of high cost, restriction, poor parent quality and low survival ratio of introduced paralichthys lethostigma parent.

The invention relates to the plant hybridization breeding and cultivation technology and provides a method for shortening the production period of mango. The method is characterized by comprising the following steps: 1) sowing seedlings and transplanting; 2) selecting polyembryonic varieties to be grafted trees, performing heading-back cut to grafted trees half treetop to use as stock; 3) selecting robust seedlings for grafting, top-grafting the seedlings on the stock of grafted trees; and 4) dressing multi-effect triazole in the soil, digging shallow ridges on the two sides of the tree covers of the grafted trees after grafting, irrigating multi-effect triazole and earthing. The invention has the following prominent substantive features and significant progresses: 1. seedling high grafting technology is adopted in the method so as to increase the grafting survival ratio; 2. the dressing multi-effect triazole technology is used to control the spindling of branch tips of grafts, promote the branch tips for aging and create beneficial nutrient conditions for advancing blossom and fruiting and promoting fruit-bearing; 3. the invention is simple and practical, potential hybrids can bear fruits after half a year so that potential hybrids and false hybrids can be identified directly and fast and the breeding process of mango is greatly shortened; and 4. the method of the invention can be popularized to the mango production so that the economic benefit of the method is remarkable owning to early fruiting and appearing on the market.

The invention discloses a raft type breeding suspending cage for patinopecten yessoensis. The raft type breeding suspending cage comprises a plurality of layered plates (1) and a net jacket (2) located at the periphery of the layered plates (1), wherein every two adjacent layered plates (1) and the net jacket at the periphery of the corresponding two layered plates (1) commonly form a suspending cage layer (3). The raft type breeding suspending cage is characterized in that the layered plates (1) are hard nylon netted plates, middle netted plates (4) which are vertical to the layered plates (1) and equally divide the layered plates (1) are arranged in the suspending cage layers (3), and the net jacket is formed through weaving smooth nylon mesh wires. The breeding suspending cage has the advantages that the structure is simple, the design is ingenious, the survival ratio of patinopecten yessoensis can be increased, and the cleaning and patinopecten yessoensis loading / unloading are convenient.

The invention discloses a method for improving the survival rate of afforestation in mountainous areas: from late February to early March, tree holes are dug in mountainous areas according to a certain row spacing, and cake fertilizer or compound fertilizer is applied in the tree holes. Select the seedlings suitable for mountain afforestation, add polyvinyl alcohol with water and soil to make mud before planting, dip the roots of the seedlings into the mud and dip the roots, and plant them as you dip, and pour enough water to fix the roots after planting; Place 2 fertilizer bags with organic fertilizers, so that the planting hole can always keep moist, and the plants can continuously absorb the inflowing nutrients, ensuring the rapid growth of the plants under the sufficient supply of fertilizer and water. A method for improving the survival rate of mountain afforestation of the present invention not only increases the survival rate of afforestation to 81-97%, but also overcomes the disadvantages of low afforestation survival rate and slow forest formation caused by the traditional planting mode, and can advance the mountainous area by 3 ~5 years to achieve the expected disaster resistance and landscape effects.

The invention discloses a method for water-fed forestation in arid hillside fields of arid-hot river valleys, pertaining to the technical field of water-saving agriculture. The method comprises the steps of digging large ponds, backfilling prepared fertilizers, cultivating, strengthening and field planting seedlings, timely field planting against the rain in rain season, covering with grass to keep water, top dressing in rain season to promote the seedlings, wherein, the length, the width and the depth of the planting hole of an arbor tree is 120 cm multiplied by 80 cm multiplied by 100 cm while a frutescose tree is 80 cm multiplied by 50 cm multiplied by 60 cm and the planting hole is perpendicular to slope direction; when backfilling the soil, the soil in each pond is mixed with 5 kg to 10 kg of organic fertilizer and 2 kg to 4 kg of mixed fertilizer of calcium, magnesium and phosphate fertilizers; field planting is carried out in May to June with sufficient rain water; after the field planting of nursery stocks, the weeds are cut in time for covering the tree pond; approximately 20 days after the field planting of the nursery stocks, quick effective nitrogen fertilizer is applied in proper time. Adoption of the method of the invention can enhance the survival ratio of forestation in arid hillside fields of arid-hot valleys under the circumstances of no irrigation water to more than 95 percent and the preservation rate to more than 90 percent. The technology of the invention is scientific, reasonable and feasible and has high significance in revegetation and ecological reconstruction of arid-hot river valleys.

A plant nutrient is made from the following components with the weight percentages: 50%-100% of animal blood, 0-40% of ferrous sulfate, 0-10% of ferric sulfate, 0-2% of zinc sulfate, 0-2% of boracic acid, 0-2% of manganese sulfate, 0-10% of calcium superphosphate, 0-10% of potassium dihydrogen phosphate and 0-2% of copper sulfate. The application method of the plant nutrient is as follows: all the components are made into an aqua, paste or powder, and injected into plants by perforating holes on the plants. The plant nutrient of the invention has the advantages of available raw materials, simple processing, convenient and safe use as well as no environmental pollution, and the scattered animal blood is fully utilized. The plant nutrient is applicable to every growth and development period of the plants. By adding mineral substances needed by plants according to the growth and development situations of the plants, the efficiency of the plant nutrient can be enhanced, and the nutrient deficiency symptom of the plants can be prevented and treated. By adopting the plant nutrient during the transplant of big trees, the transplant survival ratio can be increased, and the recovery time of tree crowns can be greatly shortened.

The invention relates to a liquid feed which can enhance the survival rate and growing performance of newborn piglets, and is prepared by components with the following weight percentage content of 50-60 percent of fresh milk, 3-4 percent of condensed whey powder, 5-10 percent of lactose, 0.5-1 percent of swine plasma protein powder, 25-35 percent of water, 0.3-0.5 percent of lecithin, 4-5 percent of soybean oil, 0.02-0.05 percent of emulsifier, 0.02-0.05 percent of multi-vitamin, 0.5-1 percent of citric acid and 800-1000IU percent of Gentamicin. The lactose and fat in the liquid feed of the invention furthest enhance nutrition intake amount of suckling piglets. Due to the combining use of nutrition supplementary liquid and an acidifier and antibiotics, the invention not only increases the nutrition intake, but also increases stomach and intestine acidity and promotes the health of the piglets.

The invention provides a method for reproducing magnolia zenii seedlings. The method comprises the following steps that in June, a magnolia zenii branch which is half lignified and with a top leave is clipped by 8-10cm, and a half of a blade of the top leave is clipped; the lower portion of the magnolia zenii branch in the step (1) is immersed in rooting agent solution and then treated; the magnolia zenii branch in the step (2) is inserted to a cutting medium, Topsin medicinal liquid is used for spraying the cutting medium, and the cutting medium with the magnolia zenii branch is placed in a greenhouse with a sunshade net for culturing. The method is simple in process, branches with the proper lignification degree, kinds and concentration of rooting agents and proper cutting media are selected, the rooting ratio can reach more than 75%, therefore, the cutting survival ratio of the magnolia zenii seedlings is greatly improved, the magnolia zenii seedlings can be bred in a large-scale mode, the cost is low, and the efficiency is high.

The present invention provides, inter alia, methods for identifying a candidate agent that may be effective to treat or ameliorate an effect of a neurodegenerative disease in a subject. These methods include: (a) contacting a wildtype neuron and a mutant neuron with a stressor which is effective to accelerate the degeneration of the mutant neuron; (b) further contacting the wildtype neuron and the mutant neuron from step (a) with a candidate agent; and (c) determining whether the candidate agent lowers a wildtype to mutant survival ratio or increases both wildtype and mutant neuron survival.

The invention belongs to the field of the pharmaceutical chemistry and pharmacology, and concretely relates to an application of a compound of [(1E,1'E)-(4-Oxo-1-propionylpiperidine-3,5-diylidene)bis(methanylylidene)]bis(2-methoxy-4,1-phenylene)dipropionate (S1) in the preparation of anti-inflammation or / and anti-oxidation drugs. The above piperidone type curcumin analog S1 can dose-dependently inhibit the expression and release of inflammation factors comprising TNF-alpha, IL-6 and the like, and can also obviously improve the survival rate of mice death-induced by LPS. The compound has a good safety and stability, and can be exploited to inflammation or / and oxidation stress related disease treatment drugs.