56 results about "Patinopecten yessoensis" patented technology

The present invention relates to a breeding method of white scallop new line. Said breading method includes the following several procedures: parent scallop cultivation, promoting oviposition, spat seed breeding, shell color identification and screening and secondary shell color selection and breeding.

The invention relates to a parent patinopecten yessoensis culturing and hatching method, and belongs to the technical field of parent patinopecten yessoensis culturing and hatching methods. The parent patinopecten yessoensis culturing and hatching method is characterized by mainly comprising the following steps of: (1) selecting and conveying parent patinopecten yessoensis; (2) supplying a culturing condition of the parent patinopecten yessoensis; (3) culturing and hatching high-quality parent patinopecten yessoensis, namely (1) putting the parent patinopecten yessoensis into a pool, (2) heating, (3) feeding baits, (4) changing water and reversing the pool, (5) inflating, (6) adding beneficial bacteria, and (7) performing oviposition and hatching. According to the parent patinopecten yessoensis culturing and hatching method, the technology is scientific and reasonable; the flow operation is simple and standard; the oviposition amount of the parent patinopecten yessoensis is large; and the survival rate of young eggs is high.

The invention relates to a patinopecten yessoensis seedling culturing method, and belongs to the technical field of patinopecten yessoensis seedling culturing methods. The patinopecten yessoensis seedling culturing method is characterized by mainly comprising the following steps of: (1) performing oviposition and hatching; (2) cultivating larvas; (3) feeding substratums; and (4) cultivating young mollusk. According to the patinopecten yessoensis seedling culturing method, the technology is scientific and reasonable; the flow operation is simple and standard; the seedling survival rate is high; the seedlings are robust; and a good foundation is laid for growth of patinopecten yessoensis in the future.

The invention provides an SNP locus related to growth characteristics of patinopecten yessoensis. The locus is the 1054 site of IGFBP (Insulin Like Growth Factor Binding Proteins) genes of which the nucleotide sequence is shown as SEQ ID NO:2 and has the base of A or G. The invention also provides a probe for detecting the SNP locus and a parting primer. The transcription sequence of the IGFBP genes in the patinopecten yessoensis is subjected to sequencing and Clustal comparison, and three SNP loci are screened. The site polymorphism is detected in the patinopecten yessoensis group by using a high resolution melting curve technology, and correlation between the site genotype frequency and the growth characteristics of patinopecten yessoensis is analyzed. The locus C.1054A>G is obviously related to important growth characteristics such as the height, shell length, weight, soft weight and adductor muscle weight of the patinopecten yessoensis, the growth characteristics of the AG type individuals are obviously lower than those of AA and GG type individuals (P is less than 0.05), and the characteristic value of the GG type is the highest. Therefore, individuals of which the genotype is GG on the locus can be preferentially selected during production and are used as parents for performing high-yield patinopecten yessoensis breeding or performing large-scale breeding.

The invention relates to a high-valued utilization method for patinopecten yessoensis offal. The high-valued utilization method for the patinopecten yessoensis offal is technically characterized in that the patinopecten yessoensis offal is cleanly washed, weighted, added with water and made to homogenate. Enzymolysis is carried out on the homogenate by adding trypsin and bacillus subtilis protease in the homogenate. Enzymatic hydrolysate is centrifuged, and supernate and precipitate are separated. The supernate is added with hydrogen peroxide so as to decolor the supernate, the supernate is centrifuged, the potential of hydrogen (PH) is adjusted, acidic and neutral protein is eliminated through centrifugation, and alcohol precipitation is carried out on the supernate, so that a crude product of crude glycosaminoglycan is obtained, and the yield rate is 0.265%. The crude product is dissolved by being added with water, 0.4% trypsin are added to carry out enzymolysis on the crude product for a second time, then the crude product is centrifuged, alcohol precipitation is carried out on the supernate, cetyl trimethyl ammonium bromide (CTAB) is added, and the supernate is centrifuged. The precipitate is dissolved in a sodium acetate solution, dissociation liquid is obtained and dialyzed, and alcohol precipitation is carried out on the dissociation liquid, so that highly purified glycosaminoglycan is obtained. The purity of the glycosaminoglycan can reach more than 90%. The glycosaminoglycan has good antioxygenation and can be used as a raw material of functional foods. All protein precipitate is mixed and is used as a good protein source for feeding animals. The high-valued utilization method for the patinopecten yessoensis offal has advantages that the offal of processed scallops adductor of patinopecten yessoensis is comprehensively utilized in a high-valued mode, and non-pollution and zero emission are achieved in the whole technological process.

The invention relates to a patinopecten yessoensis ternary cross breeding method. Firstly the artificial cultivation accelerated ripening is implemented for the Russian patinopecten yessoensis. Secondly the artificial cultivation accelerated ripening is implemented for the Chinese patinopecten yessoensis. At the same time, the gonad maturation is achieved. A heating mode is utilized to the above patinopecten yessoensis for induced spawning, and the sperms and the eggs are respectively collected. The hybridization is implemented between the Chinese female scallop eggs and the Russian male scallop sperms or between the Chinese male scallop sperms and the Russian female scallop eggs, thus the first filial generation Chinese-Russian or Russian-Chinese two sorts of patinopecten yessoensis colonies which can be cultivated within two years with the conventional suspended longline farming method. The Janpanese patinopecten yessoensis and the Chinese-Russian or the Russian-Chinese patinopecten yessoensis are taken as the parents for the artificial cultivation accelerated ripening. At the same time, the gonad maturation is achieved. A heating mode is utilized to stimulate the induced spawning, and the sperms and the eggs collected are respectively selected for the implementation of hybridization. Thereby the multiple Russian-Chinese-Japanese, Japanese-Chinese-Russian or Janpanese-Russian-Chinese patinopecten yessoensis colonies are obtained, which can be cultivated with the conventional suspended longline farming method. The method is simple, practical with low cost. The patinopecten yessoensis antiviral ability is enhanced. Moreover the survival rate and the livability are high. The offspring heterozygosity is also enhanced through the group fertilization.

The invention discloses a raft type breeding suspending cage for patinopecten yessoensis. The raft type breeding suspending cage comprises a plurality of layered plates (1) and a net jacket (2) located at the periphery of the layered plates (1), wherein every two adjacent layered plates (1) and the net jacket at the periphery of the corresponding two layered plates (1) commonly form a suspending cage layer (3). The raft type breeding suspending cage is characterized in that the layered plates (1) are hard nylon netted plates, middle netted plates (4) which are vertical to the layered plates (1) and equally divide the layered plates (1) are arranged in the suspending cage layers (3), and the net jacket is formed through weaving smooth nylon mesh wires. The breeding suspending cage has the advantages that the structure is simple, the design is ingenious, the survival ratio of patinopecten yessoensis can be increased, and the cleaning and patinopecten yessoensis loading/unloading are convenient.

The invention provides a cultivation method using double-white-shell Patinopecten yessoensis Jay and ordinary purple-brown-shell Patinopecten yessoensis Jay as parent pectinid through single cross and group cross as well as the corresponding breeding methods to obtain the Patinopecten yessoensis Jay with a red-white flower pattern on the shell, the red-white-flower Patinopecten yessoensis Jay can be used for studying shellfish shell colors and is used as an ideal experimental material for research of correlated characters; and meanwhile, the red-white-flower Patinopecten yessoensis Jay is used as a base material for variety breeding of the Patinopecten yessoensis Jay. The operation method provided by the invention is simple without the introduction of foreign genes by means of complex technologies such as transgenosis and has no influence on the security of living beings and environment.

The invention aims to provide a scallop heartbeat index based rapid seed selection method. In vitro non-invasive and non-destructive heartbeat detection is carried out on a scallop and the obtained scallop heartbeat frequency and waveform serve as the bases for rapid seed selection of the scallop. The method combines the infrared sensing technology, the filter technology and the waveform amplification technology, so that the heartbeat frequency and waveform of the scallop can be rapidly and sensitively captured and detected. Compared with traditional methods, the method has the characteristics of non-invasiveness, non-destructiveness, long-term tracking, and the like, and uses scallop heartbeat as the rapid seed selection index for the first time. A research basis can be provided for breeding of patinopecten yessoensis resistant varieties and establishment of a resistance index system by adopting the method.

The invention belongs to the technical field of shellfish cultivation, and particularly relates to a sea-bottom ear-hanging cultivation method and device for patinopecten yessoensis. The method and device break through thinking limits of traditional buoyant raft and bottom sowing cultivation of patinopecten yessoensis, and an ear-hanging cultivation mode is converted into sea-bottom frame-type cultivation from sea-surface buoyant raft cultivation. According to the cultivation method and device, changes of water layers are adopted, and phenomena of more attachments and high summer death rate in surface cultivation are effectively reduced; a metal framework is adopted to serve as a cultivation support, the device is a flexible assembly and convenient to disassemble, components can be replaced after being damaged, material storage areas are saved and the cultivation cost is reduced; cultivation vessels are adopted to conduct fixed-point placement of the sea-bottom cultivation frame, recovery vessels conduct fixed-point recovery, the accurate placement is achieved, catching is accurate, the method and device avoid the problems that the utilization rate of sea area is low due to bottom sowing cultivation and submarine environment is severely damaged because of dragnet during bottom sowing all the year round, and the stability of cultivation is improved. According to the method and device, the new ear-hanging cultivation mode of patinopecten yessoensis is developed, industrialization, intensification and stereo cultivation are achieved, and the cultivation efficiency is improved.

The invention discloses a preparation method of a stable patinopecten yessoensis male gonad zymolyte -beta-carotene emulsion. The preparation method comprises the following steps of (1) performing enzymolysis with trypsin on patinopecten yessoensis male gonads to obtain a zymolyte peptide solution of which the hydrolysis degree is 1%, and performing freeze drying for standby application; (2) heating beta-carotene to enable the beta-carotene to dissolve in corn oil and preparing oil phases for standby application; (3) mixing the peptide solution of certain concentration with a sodium azide stock solution and Tween 20 and preparing aqueous phases for standby application; and (4) mixing the oil phases with the aqueous phases in a certain proportion, and performing high-speed dispersing; and (5) performing high-pressure homogenizing to obtain a stable patinopecten yessoensis male gonad zymolyte -beta-carotene emulsion. The patinopecten yessoensis male gonad zymolyte is used as an emulsifying agent, and the beta-carotene is entrapped, so that the patinopecten yessoensis male gonad zymolyte -beta-carotene emulsion being stable and high in nutrient value can be obtained.

The invention discloses a method for collecting high-quality patinopecten yessoensis seminal fluid, and belongs to the technical field of marine organisms. The method comprises the first step of selecting, temporally rearing and maturing the high-quality male patinopecten yessoensis, the second step of luring the male patinopecten yessoensis to discharge the seminal fluid and collecting the seminal fluid many times, and the third step of detecting the quality of the collected seminal fluid. The collecting method is simple and easy to operate, the parent patinopecten yessoensis cannot be hurt, the seminal fluid can be repeatedly collected, and the collected seminal fluid can be stored for a long time. It is only required to understand the genital glands of the scallops and the anatomical structures of the excretory organs and accurately recognize the nephridium and the nephridiopore when the seminal fluid is collected. The seminal liquid collected from one ordinary male patinopecten yessoensis each time can be 0.5-1.5 ml. Under the situations that the temperature is controlled and the sufficient nutrient is guaranteed, the gonad can be promoted to be mature and discharge the seminal fluid many times, and the seminal fluid of each patinopecten yessoensis can be repeatedly collected two to four times till the March or April.

The invention discloses a double white shells patinopecten yessoensis cultivation method. The patinopecten yessoensis individual, in which the upper shell whitening region area is more than 30% and the gonad development is plump, is firstly selected as a parent scallop. In the particular conditions such as density, temperature and so on, the accelerated ripening, the induced spawning, the artificial insemination, the hatching, the cultivation, the attachment seedling collection, the ex-storage, the intermediate seedling protection and growth, as well as the mariculture are implemented, so that the patinopecten yessoensis in which the whitening area is increased further is cultivated. Moreover the patinopecten yessoensis obtained is taken as a parent scallop to repeat the above steps. The steps are repeated for several times until a scallop seedling in which the upper and lower shells colours are entire pure white is obtained. The double white shells patinopecten yessoensis not only has the bright colour, at the same time the stress resistance enhancement is in favour of the inhibition of disease occurrence, and the perforation of parasites and other microorganisms can be reduced. With a nice appearance, the scallop is well received among consumers, and with the heat-resisting ability, the scallop can tolerant the high temperature up to 25 DEG C. With a low mortality rate which is less than 10%, the growth rate of the scallop is stronger than the general patinopecten yessoensis. Therefore, the economic benefits of farmers can be effectively increased.

The invention relates to the technical field of aquaculture, specifically to a mixed culture system for sea cucumber, shrimp and shellfish based on a deep-water net cage. The mixed culture system of the invention comprises a stereo bracket, wherein the upper part of the stereo bracket is provided with at least two layers of culture areas; the periphery of each layer of the culture area is surrounded with a netting; the bottom layer of the culture area is used for the culture of the sea cucumber; a plurality of sea cucumber blindages are fixedly arranged on the netting of the bottom layer of the culture area; other layers of the culture areas are used for culture of Patinopecten yessoensis; a plurality of longitudinal nettings are fixedly arranged between the nettings at two opposite side surfaces of the Patinopecten yessoensis culture areas; and a plurality of stereo string bags are arranged on each longitudinal netting. The mixed culture system provided by the invention is designed according to biological and ecological characteristics of the Patinopecten yessoensis and the sea cumber, can promote growth of the sea cucumber and improve culture yield per unit of the Patinopecten yessoensis, has the characteristics of simple operation and strong on-site operability and can improve culture yield and reduce environmental pressure by making full use of maritime space and complementarity of ecological niches of different varieties.

The invention relates to the technical field of shellfish culture, particularly relates to a net for fishing yessoensis, and discloses a patinopecten yessoensis beam trawlnet with elastic teeth. The patinopecten yessoensis beam trawlnet comprises a net rack and a net body. The net rack consists of a beam plane and net cranks, the beam plane is an irregular square consisting of a net lever and supports, the top of the beam plane is arc-shaped and is provided with a hanging bar, the main net cranks and the auxiliary net cranks are disposed below the beam plane, the elastic teeth are arranged on the supports in a scattered manner, and the length of each elastic tooth is larger than the height of a portion, which is protruded out of the beam plane, of each main net crank by 4-10cm. The net body consists of a net opening chain, an abdominal net and a back net, the net opening chain is fixed on the main net cranks and the auxiliary net cranks, the abdominal net is fixed on the net opening chain, and the back net is fixed on the beam plane of the net rack and connected with the abdominal net. Problems that patinopecten yessoensis is missed, pressed and damaged in a fishing process are greatly reduced, a single-net fishing quantity is increased by about 10% as compared with another fishing tool, and damage rate, casualty rate and sand content of the fished patinopecten yessoensis, oil consumption of the trawlnet and damage degree to a seabed ecological environment are greatly reduced.

The invention belongs to the technical field of aquaculture, relates to a cultivation technique of parent scallops of patinopecten yessoensis, and in particular relates to a water transportation method for ensuring living parent scallops of patinopecten yessoensis. The method comprises the following steps: selecting healthy patinopecten yessoensis at the age of sexual maturity and without pathogenic bacteria as parent scallops, washing for removing impurities on the surfaces of the parent scallops, emptying excrement in the parent scallops, putting the parent scallops into a water tank in an airtight heat-insulation cabin, transporting the parent scallops to a destination in a low-temperature environment at 2-5 DEG C, and putting the parent scallops in a fostering pond at the water temperature of 3-7 DEG C for buffering and temporary keeping. By virtue of the method, the living transportation time reaches 72 hours, and the survival rate of the parent scallops is up to 99% and above. According to the method, the process is simple, the transportation cost is low, the equipment can be reused, and the operation and popularization are facilitated. The method can be used for effectively solving the bottleneck problem of high death rate during long distance transportation in processes of cultivating of fry of patinopecten yessoensis, introduction of fine varieties from abroad and marketing, and thus has important significance in promoting upgrading and low-carbon and energy-saving healthy sustainable development of industry of patinopecten yessoensis.

The invention discloses a method for simultaneously extracting two subtypes of metallothionein from Patinopecten yessoensis, belonging to the technical field of metallothionein extraction. The two subtypes of metallothionein are extracted from the viscera of Patinopecten yessoensis by using the method comprising steps consisting of zinc chloride induction, multiple centrifugation extraction, primary purification via a molecular sieve prepacked column with polymethacrylate as a filling material, and secondary purification via anion exchange chromatography. A mixture of the two subtypes and individual products of the two subtypes obtained by using the method for simultaneously extracting two subtypes of metallothionein from Patinopecten yessoensis in the invention have high purity. Moreover,the extraction process of the method is simple; and the viscera of Patinopecten yessoensis are used as a raw material for the extraction of the metallothionein, and the processing leftovers of enterprises are fully utilized, and the economic value of Patinopecten yessoensis is improved.

The invention discloses a method for preparing a functional flavored base material through gonads of patinopecten yessoensis. The method comprises the following steps of pretreating the gonads of the patinopecten yessoensis, performing fat extraction, drying defatted raw materials to obtain an enzymolysis product, performing a Maillard reaction on the enzymolysis product to obtain a derivative, and enabling the fat, the enzymolysis product and the derivative to be in compatibility so as to obtain the functional flavored base material. According to the method disclosed by the invention, a defatting technique, a biological enzymolysis technique and a Maillard modifying method are adopted for preparing the functional flavored base material, so that the flavor of the product namely the functional flavored base material is guaranteed, the nutrient value is reserved, the requirements of modern people for diversified meals can be met, and the functional flavored base material has the characteristics of being natural in raw material, high in nutritive activity of the product and the like. The method disclosed by the invention is reasonable in technology and convenient to operate, and has important significance on development and utilization of patinopecten yessoensis raw materials.

The invention relates to an aquaculture method of shells, in particular to an inductive agent for inducing marine bivalve mollusk to produce eggs as well as an application method thereof. The application method comprises the following steps: taking cooked egg yolk liquid obtained through bolting-silk treatment as the inductive agent, splashing the inductive agent in a parent shellfish pool uniformly according to the concentration, being 1.0 to 1.5 g/m<3>, of the cooked egg yolk under the conditions that the water temperature is 20 to 30 DEG C, the salinity is 25 to 35 and the pH value is 7.5 to 8.3, treating for 0.5 to 1 hour, performing pool separation according to a siphonage method after the parent shellfishes lay eggs, fully filling the pool with water again, and diluting the concentration of cooked egg yolk. Compared with other stimulation methods, the method has the following advantages: the egg producing efficiency can be increased by 30 to 50 percent and the egg producing efficiency of the parent shellfishes can be effectively improved. The inductive agent can be applied to various marine bivalve mollusk such as hard-shelled clam, clam, short-necked clam, cyclina sinensis gmelin, bay scallop, chlamys farreri and patinopecten yessoensis.

The invention relates to a device for breeding patinopecten yessoensis at the bottom of a sea and a breeding method thereof and belongs to the technical field of patinopecten yessoensis aquaculture. The device for breeding patinopecten yessoensis at the bottom of the sea comprises a rectangular frame I provided with grid ropes I, a rectangular frame II provided with grid ropes II and four connecting pieces. The four connecting pieces make four corners of the rectangular frame I and four corners of the rectangular frame II connected to form a shape of four pyramid frustums. The top of a net cage is connected with the rectangular frame I and the grid ropes I, and the bottom of the net cage is connected with the rectangular frame II and the grid ropes II. The device for breeding patinopectenyessoensis at the bottom of the sea can be used at the mud bottom or sandy bottom, attachment of attachment objects such as mussels is effectively avoided, a water environment is relatively stable dueto low aquaculture water temperature, and improvement of the patinopecten yessoensis survival rate is facilitated.

The invention relates to a low-temperature preserving fluid for patinopecten yessoensis sperms, a preserving method and a using method, and belongs to the field of aquaculture. The low-temperature preserving fluid comprises the following components by mass percent: 0.02 g/L of florfenicol, 0.02 g/L of compound sulfamethoxazole, 26.72 g/L of sodium chloride, 0.72 g/L of potassium chloride, 1.15 g/L of calcium chloride and 0.20 g/L of sodium bicarbonate, and a solvent is sterile seawater. The invention further provides a method for preserving the patinopecten yessoensis sperms by using the low-temperature preserving fluid and a using method. The patinopecten yessoensis sperms can be preserved at the low temperature of 4 DEG C, centrifugation cannot be carried out in the sperm collecting process, centrifugation can cause the motility of the patinopecten yessoensis sperms to be reduced, and the final fertilization rate is affected. The sperms preserved for 5 days by using the preserving fluid disclosed by the invention are inseminated, the sperm-egg ratio is 10000: 1, and the fertilization rate is 85% or above.

The invention provides a method for identifying central centromere chromosomes of patinopecten yessoensis. By combining genome sequence information, SNP linkage map information and fosmid monoclonal decoding information of patinopecten yessoensis, three labels distributed on a SNP linkage map of the patinopecten yessoensis are selected to screen a fosmid library, screened clones containing SNP sites are utilized to prepare probes with fluorescent labels, then fluorescent in-situ hybridization and co-hybridization are carried out by using the probes, and the specific recognition of the three pairs of central centromere chromosomes of the scallop according to the distribution of the fluorescent signals of the three specific clones containing the SNP labels on the scallop chromosomes. The three labels achieve identification of the three pairs of central centromere chromosomes and have important significance differentiation and identification of scallop yessoensis chromosomes, constructionof cytogenetic maps and evolution analysis among chromosomes from different species.

The invention discloses an automatic processing device for patinopecten yessoensis aquaculture. The automatic processing device comprises a patinopecten yessoensis shell leveling tail part positioningdevice and a suction disc regulation positioning mechanism, wherein the patinopecten yessoensis shell leveling tail part positioning device comprises a leveling positioning machine frame, a rodless air cylinder for positioning, a first patinopecten yessoensis conveying mechanism, a second patinopecten yessoensis conveying mechanism, a patinopecten yessoensis shell flat pressing mechanism, a patinopecten yessoensis clamping and positioning device and a patinopecten yessoensis tail part positioning device; the suction disc regulation positioning mechanism comprises a positioning vertical frameand a rotatary suction disc assembly; the rotating suction disc assembly is used for adsorbing a patinopecten yessoensis upper shell and driving patinopecten yessoensis to rotate; the upper end of thepositioning vertical frame is provided with the rotary suction disc assembly through a suction disc ascending and descending assembly. The structural design is reasonable; the patinopecten yessoensiscan be levelled; the tail part of the patinopecten yessoensis can be precisely positioned; great convenience is provided for the subsequent patinopecten yessoensis processing.