High-valued utilization method for patinopecten yessoensis offal

A technology for shrimp scallops and leftovers, which is applied in the field of high-value utilization of shrimps and scallops, can solve the problems of low utilization rate of leftovers, waste of resources, etc., and achieves high added value of products, shortened time, and mild reaction conditions. Effect

Inactive Publication Date: 2013-06-05
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
View PDF6 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the utilization rate of leftovers from pr...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0037] ⑴Raw material processing: After washing the scallop scraps, add 2 times the weight of distilled water to homogenate to make a homogenate;

[0038] (2) Enzymolysis: Add trypsin and Bacillus subtilis neutral protease to the above homogenate, the amount of enzyme added is 0.8% of the total weight of the waste, and trypsin and Bacillus subtilis neutral protease each account for half. Enzymolysis for 3.5 hours; separate the supernatant: boil the enzymolysis solution to kill the enzyme, centrifuge at 5000r / min for 10min, separate the supernatant and precipitate;

[0039] (3) Decolorization: add 5% hydrogen peroxide to the supernatant to decolorize;

[0040] (4) Remove protein: first adjust the pH to 2.0 with HCl, and remove the precipitate by centrifugation; then adjust the pH to 7.0 with NaOH, and remove the precipitate by centrifugation;

[0041] (5) Alcohol precipitation method to extract crude glycosaminoglycans: slowly add ethanol to the supernatant under constant stirr...

example 2

[0048] ⑴Raw material processing: After washing the scallop scraps, add 3 times the weight of distilled water to homogenate to make a homogenate;

[0049] (2) Enzymolysis: Add trypsin and Bacillus subtilis neutral protease to the above homogenate, the amount of enzyme added is 1% of the total weight of the waste, and trypsin and Bacillus subtilis neutral protease account for half each. Enzymolysis for 4 hours; separation and supernatant: after boiling the enzymolysis solution to kill the enzyme, centrifuge at 5000r / min for 10min, and separate the supernatant and precipitate;

[0050] (3) Decolorization: add 7% hydrogen peroxide to the supernatant to decolorize;

[0051] (4) Remove protein: first adjust the pH to 2.0 with HCl, and remove the precipitate by centrifugation; then adjust the pH to 7.0 with NaOH, and remove the precipitate by centrifugation;

[0052] ⑸ Alcohol precipitation method to extract crude glycosaminoglycans: slowly add ethanol to the supernatant under const...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a high-valued utilization method for patinopecten yessoensis offal. The high-valued utilization method for the patinopecten yessoensis offal is technically characterized in that the patinopecten yessoensis offal is cleanly washed, weighted, added with water and made to homogenate. Enzymolysis is carried out on the homogenate by adding trypsin and bacillus subtilis protease in the homogenate. Enzymatic hydrolysate is centrifuged, and supernate and precipitate are separated. The supernate is added with hydrogen peroxide so as to decolor the supernate, the supernate is centrifuged, the potential of hydrogen (PH) is adjusted, acidic and neutral protein is eliminated through centrifugation, and alcohol precipitation is carried out on the supernate, so that a crude product of crude glycosaminoglycan is obtained, and the yield rate is 0.265%. The crude product is dissolved by being added with water, 0.4% trypsin are added to carry out enzymolysis on the crude product for a second time, then the crude product is centrifuged, alcohol precipitation is carried out on the supernate, cetyl trimethyl ammonium bromide (CTAB) is added, and the supernate is centrifuged. The precipitate is dissolved in a sodium acetate solution, dissociation liquid is obtained and dialyzed, and alcohol precipitation is carried out on the dissociation liquid, so that highly purified glycosaminoglycan is obtained. The purity of the glycosaminoglycan can reach more than 90%. The glycosaminoglycan has good antioxygenation and can be used as a raw material of functional foods. All protein precipitate is mixed and is used as a good protein source for feeding animals. The high-valued utilization method for the patinopecten yessoensis offal has advantages that the offal of processed scallops adductor of patinopecten yessoensis is comprehensively utilized in a high-valued mode, and non-pollution and zero emission are achieved in the whole technological process.

Description

technical field [0001] The invention belongs to the technical field of food, and relates to high-value utilization of leftovers, in particular to a method for high-value utilization of Ezo scallop leftovers. Background technique [0002] Ezo scallops are native to Japan, the southern waters of the Kuril Islands in Russia, Hokkaido in Japan and the northern part of Honshu. They are large cold-water shellfish. An important species of shellfish aquatic products. Ezo scallops are rich in unsaturated fatty acids EPA and DHA, which also have the functions of nourishing yin and nourishing the kidney, and have good curative effects on diseases such as weakness, loss of appetite, and malnutrition. The adductor muscle of Ezo scallop is thick, the meat is delicious and nutritious, and it is widely used to make shellfish sticks to meet the needs of the market. At present, the utilization rate of leftovers from processing shellfish is low, resulting in waste of resources. The inventio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C08B37/00A23K1/10
Inventor 袁春营崔青曼李广靖
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap